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    METHOD FOR CULTURING CARDIAC PROGENITOR CELLS AND USE OF CARDIAC PROGENITOR CELLS
    64.
    发明申请
    METHOD FOR CULTURING CARDIAC PROGENITOR CELLS AND USE OF CARDIAC PROGENITOR CELLS 审中-公开
    培养心脏病原体细胞的方法和使用心脏原细胞细胞

    公开(公告)号：US20130295060A1

    公开(公告)日：2013-11-07

    申请号：US13886663

    申请日：2013-05-03

    申请人： Inje University Industry-Academic Cooperation Foundation ,  Ewha University-Industry Collaboration Foundation

    发明人： Young Il YANG ,  Seung Jin Lee ,  Hyeong In Kim ,  Jong Tae Kim ,  Soon Ho Cheong

    IPC分类号： A61K35/34

    CPC分类号： A61K35/34 ,  C12N5/0657 ,  C12N5/0692 ,  C12N2501/999 ,  C12N2533/52

    摘要： Disclosed is a method for culturing myocardium-resident cardiac progenitor cells, comprising: embedding myocardial fragments into hydrogel; culturing the myocardial fragment into hydrogel; degrading only the hydrogel to recover cardiac progenitor cells grown out of the myocardial fragment to the hydrogel; and amplifying the cardiac progenitor cells in vitro. Also, the cardiac progenitor cells, a method for differentiating the same, and the use thereof as cell therapeutic agent for heart diseases are provided. In addition to possessing the potential to differentiate into cardiomyocytes, osteoblasts, adipocytes, chondrocytes, vascular endothelial cells, smooth muscle cells, neural cells, and skeletal muscle cells, the myocardium-resident cardiac progenitor cells can spontaneously differentiate into cardiomyocytes even in the absence of a special differentiation inducing agent. Thus, the cardiac progenitor cells can be used to produce bio-active medicines such as cell therapeutics and tissue engineering therapeutics with high industrial applicability.

    摘要翻译： 公开了一种培养心肌驻留心脏祖细胞的方法，包括：将心肌片段包埋在水凝胶中; 将心肌片段培养成水凝胶; 仅降解水凝胶以将从心肌片段生长的心脏祖细胞回收至水凝胶; 并在体外扩增心脏祖细胞。 此外，提供了心脏祖细胞，其分化方法及其作为心脏病的细胞治疗剂的用途。 除了具有分化成心肌细胞，成骨细胞，脂肪细胞，软骨细胞，血管内皮细胞，平滑肌细胞，神经细胞和骨骼肌细胞的潜力之外，心肌驻留的心脏祖细胞也可以自发分化成心肌细胞，即使没有 一种特殊的分化诱导剂。 因此，心脏祖细胞可用于生产具有高工业应用性的生物活性药物，例如细胞治疗剂和组织工程治疗剂。

    PLASMA TREATMENT APPARATUS USING LEAKAGE CURRENT TRANSFORMER
    66.
    发明申请
    PLASMA TREATMENT APPARATUS USING LEAKAGE CURRENT TRANSFORMER 审中-公开
    使用泄漏电流互感器的等离子体处理装置

    公开(公告)号：US20130175927A1

    公开(公告)日：2013-07-11

    申请号：US13822745

    申请日：2011-09-16

    申请人： Je Won Lee

    发明人： Je Won Lee

    IPC分类号： H05H1/24

    CPC分类号： H05H1/24 ,  H01J37/32009

    摘要： Provided is a plasma treatment apparatus using a leakage current transformer, the apparatus, including: a chamber which provides a closed space in which plasma is formed, and receives a treated sample in an inner part thereof; an exhaust unit for forming the inner part of the chamber in a vacuum state; plasma generation electrodes fixed in the chamber, positive and negative poles of which are installed to be opposed to each other; a variable power supply which is installed in an outer part of the chamber and supplies a power source to the plasma generation electrodes; and a leakage current transformer which is installed between the variable power supply and the plasma generation electrodes and adjusts voltage and current applied to the plasma generation electrodes.

    摘要翻译： 提供一种使用漏电流互感器的等离子体处理装置，该装置包括：提供形成等离子体的封闭空间的腔室，并在其内部容纳经处理的样品; 排气单元，用于在真空状态下形成所述室的内部; 等离子体产生电极固定在腔室中，其正极和负极彼此相对安装; 可变电源，其安装在所述室的外部部分中并将电源供应到所述等离子体产生电极; 以及安装在可变电源和等离子体发生电极之间的泄漏电流互感器，并且调节施加到等离子体发生电极的电压和电流。

    PROMOTER FOR THE HIGH LEVEL EXPRESSION IN PLANT-TISSUE CULTURE AND VECTOR USING THE SAME
    68.
    发明申请
    PROMOTER FOR THE HIGH LEVEL EXPRESSION IN PLANT-TISSUE CULTURE AND VECTOR USING THE SAME 失效
    使用植物组织培养的高水平表达的推广者和使用它的矢量

    公开(公告)号：US20100100986A1

    公开(公告)日：2010-04-22

    申请号：US12596200

    申请日：2008-08-12

    申请人： Gyung Hye Huh ,  Young Hwa Kim ,  Bo Mi Kim

    发明人： Gyung Hye Huh ,  Young Hwa Kim ,  Bo Mi Kim

    IPC分类号： A01H5/00 ,  C07H21/00 ,  C12N15/82 ,  C07H21/04

    CPC分类号： C12N15/8223

    摘要： Disclosed herein are a promoter (SEQ ID NO.: 1) inducing high level expression of a target gene in plant tissue cultured cells, derived from the sweetpotato gene of Ran GTPase, small GTP binding protein, a plant transformation vector for carrying the same and a method for expressing a foreign gene in plant cell using the vector. The activity of promoter according to the present invention is higher than that of universal CaMV 35S promoter in transgenic suspension cultured cell lines, calluses and adventitious roots. Thus, the promoter is useful in the generation of transgenic cell lines including cultured roots to produce valuable materials such as medicinal or industrial proteins in a large quantities with plant tissue cultured cells.

    摘要翻译： 本文公开了诱导来自Ran GTPase的甘薯基因的植物组织培养细胞中靶基因的高水平表达的启动子（SEQ ID NO：1），小GTP结合蛋白，用于携带其的植物转化载体， 使用载体在植物细胞中表达外源基因的方法。 根据本发明的启动子的活性高于转基因悬浮培养细胞系，愈伤组织和不定根中通用CaMV 35S启动子的活性。 因此，该启动子可用于生成包括培养根的转基因细胞系，从而以植物组织培养的细胞大量生产有价值的材料，例如药用或工业蛋白质。