公开(公告)号：US07465855B2

公开(公告)日：2008-12-16

申请号：US11068602

申请日：2005-02-28

申请人： Charles P. West ,  Edgar L. Piper

发明人： Charles P. West ,  Edgar L. Piper

IPC分类号： A01H5/00 ,  A01H5/10 ,  A01H15/00 ,  A01N65/00 ,  C12N1/02

CPC分类号： A01N63/04 ,  Y10S435/911

摘要： A grass with improved environmental stress tolerance and low animal toxicity is disclosed where the grass is infected with an isolated endophyte selected from the groups consisting of USDA-PI no. 269850, Ijoukak, Morocco 92060, Boumalne-de-Dades, Morocco KBG 5921, USDA-PI no. 516560, Oujda, Morocco KBG 5892, and mixtures and combinations thereof. A method for stably infecting the grasses is also disclosed.

摘要翻译： 公开了具有改善的环境胁迫耐受性和低动物毒性的草，其中草被分离的内生真菌感染，所述分离的内生真菌选自USDA-PI No. 269850，Ijoukak，Morocco 92060，Boumalne-de-Dades，Morocco KBG 5921，USDA-PI no。 516560，Oujda，Morocco KBG 5892，以及它们的混合物和组合。 还公开了一种用于稳定感染草的方法。

公开(公告)号：US07465537B2

公开(公告)日：2008-12-16

申请号：US11119587

申请日：2005-05-01

申请人： Kevin D. Raney ,  Craig E. Cameron ,  Bhuvanesh Dave ,  Joshua Sakon ,  Jeff Zhiqiang Lu ,  Samuel G. Mackintosh ,  Thomas A. Jennings

发明人： Kevin D. Raney ,  Craig E. Cameron ,  Bhuvanesh Dave ,  Joshua Sakon ,  Jeff Zhiqiang Lu ,  Samuel G. Mackintosh ,  Thomas A. Jennings

IPC分类号： C12Q1/68 ,  C12N4/48

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N2710/10343 ,  C12N2770/24222 ,  C12Q1/6883 ,  G06F19/16

摘要： The inventors have discovered that an ATPase-deficient dominant-negative mutant NS3 protein of hepatitis C virus inhibits activity of the wild-type NS3 protein and inhibits replication of hepatitis C virus (HCV). The solved crystal structure of a multi-enzyme NS3 complex on a DNA substrate is also provided. The inventors have tested a peptide matching the sequence of a portion of NS3 that interacts with another NS3 molecule for inhibiting HCV replication. The peptide inhibits HCV replication. Accordingly, the invention provides a method of inhibiting HCV replication in cells infected with HCV involving transforming the cells with a vector expressing a dominant-negative mutant NS3 gene. The invention also provides a method of inhibiting HCV replication in cells infected with HCV involving administering to the cells a dominant-negative mutant NS3 protein. The invention also provides peptides and agents that inhibit HCV replication and methods of identifying agents that inhibit HCV replication.

摘要翻译： 本发明人已经发现，丙型肝炎病毒的ATP酶缺陷型显性失活突变体NS3蛋白抑制野生型NS3蛋白的活性并抑制丙型肝炎病毒（HCV）的复制。 还提供了DNA酶底物上多酶NS3复合物的溶解晶体结构。 发明人已经测试了与NS3的一部分序列匹配的肽，其与另一NS3分子相互作用以抑制HCV复制。 肽抑制HCV复制。 因此，本发明提供了抑制HCV感染细胞中HCV复制的方法，涉及用表达显性失活突变体NS3基因的载体转化细胞。 本发明还提供了一种在感染HCV的细胞中抑制HCV复制的方法，包括向细胞施用显性失活突变体NS3蛋白。 本发明还提供抑制HCV复制的肽和试剂以及鉴定抑制HCV复制的药剂的方法。

公开(公告)号：US07371736B2

公开(公告)日：2008-05-13

申请号：US10931780

申请日：2004-09-01

申请人： John D. Shaughnessy ,  Bart Barlogie ,  Fenghuang Zhan

发明人： John D. Shaughnessy ,  Bart Barlogie ,  Fenghuang Zhan

IPC分类号： A61K39/00 ,  C12Q1/68 ,  A01N43/04 ,  A01N57/00

CPC分类号： C12Q1/6886 ,  C12Q1/6837 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/158 ,  Y02A90/26

摘要： Gene expression profiling reveals four distinct subgroups of multiple myeloma that have significant correlation with various clinical characteristics. Diagnosis for multiple myeloma (and possibly monoclonal gammopathy of undetermined significance) based on differential expression of 14 genes, as well as prognosis for the four subgroups of multiple myeloma based on the expression of 24 genes are established. A 15-gene model that classifies myeloma into 7 groups is also reported. Gene expression profiling also allows placing multiple myeloma into a developmental schema parallel to that of normal plasma cell differentiation. Development of a gene expression- or developmental stage-based classification system for multiple myeloma would lead to rational design of more accurate and sensitive diagnostics, prognostics and tumor-specific therapies for multiple myeloma.

摘要翻译： 基因表达分析显示与各种临床特征有显着相关性的多个骨髓瘤的四个不同亚组。 基于14个基因的差异表达的多发性骨髓瘤的诊断（可能是未确定的意义的单克隆丙种球蛋白病），以及基于24个基因的表达的多发性骨髓瘤的四个亚组的预后。 还报道了将骨髓瘤分为7组的15基因模型。 基因表达谱也允许将多发性骨髓瘤放置在与正常浆细胞分化平行的发育模式中。 开发基因表达或基于发育阶段的多发性骨髓瘤分类系统将导致对多发性骨髓瘤的更准确和敏感的诊断，预后和肿瘤特异性治疗的合理设计。

公开(公告)号：US07273904B2

公开(公告)日：2007-09-25

申请号：US10679247

申请日：2003-10-03

申请人： Xiaogang Peng ,  Haiyan Chen ,  Wenzhou Guo ,  Y. Andrew Wang

发明人： Xiaogang Peng ,  Haiyan Chen ,  Wenzhou Guo ,  Y. Andrew Wang

IPC分类号： C08K3/30 ,  H01L21/76 ,  C01B21/064

CPC分类号： B82Y30/00 ,  B22F2999/00 ,  B82Y15/00 ,  C01B9/00 ,  C01B13/145 ,  C01B19/007 ,  C01G11/02 ,  C01P2002/82 ,  C01P2002/84 ,  C01P2002/86 ,  C01P2002/89 ,  C01P2004/04 ,  C01P2004/64 ,  C01P2004/80 ,  C01P2006/37 ,  C09K11/02 ,  C09K11/565 ,  C09K11/883 ,  C30B7/00 ,  C30B7/005 ,  C30B29/605 ,  G01N33/545 ,  G01N33/588 ,  B22F1/0007 ,  B22F1/0018

摘要： Dendron ligands or other branched ligands with cross-linkable groups were coordinated to colloidal inorganic nanoparticles, including nanocrystals, and substantially globally cross-linked through different strategies, such as ring-closing metathesis (RCM), dendrimer-bridging methods, and the like. This global cross-linking reaction sealed each nanocrystal within a dendron box to yield box-nanocrystals which showed dramatically enhanced stability against chemical, photochemical and thermal treatments in comparison to the non-cross-linked dendron-nanocrystals. Empty dendron boxes possessing a very narrow size distribution were formed by the dissolution of the inorganic nanocrystals contained therein upon acid or other etching treatments.

摘要翻译： Dendron配体或具有可交联基团的其它支链配体与胶体无机纳米颗粒（包括纳米晶体）配位，并通过不同的策略（例如闭环复分解（RCM）），树枝状聚合物桥接方法等进行基本全局交联。 这种全球交联反应将每个纳米晶体密封在树枝状体内以产生盒状纳米晶体，与非交联树枝状纳米晶体相比显示出显着增强的化学，光化学和热处理稳定性。 通过在酸或其它蚀刻处理中所含的无机纳米晶体的溶解形成具有非常窄尺寸分布的空的树枝状结构。

公开(公告)号：US20070196392A1

公开(公告)日：2007-08-23

申请号：US11708620

申请日：2007-02-20

申请人： Catherine M. O'Connell

发明人： Catherine M. O'Connell

IPC分类号： A61K39/118 ,  C12N1/20

CPC分类号： A61K39/118 ,  A61K2039/522 ,  C12N1/36 ,  C12N15/74 ,  C12R1/01

摘要： The invention involves the discovery that Chlamydia sp. strains can be cured of their plasmids by treatment with novobiocin, and that plasmid-deficient strains are defective in infecting cells under standard conditions, but can infect cells if centrifuged onto the host cells. But it is found that plasmid-deficient strains with wild-type infection efficiency under standard conditions can be isolated as mutants from parent plasmid-deficient strains with low infectivity by selecting for infection under standard conditions. Both the less infective and the highly infective plasmid-deficient strains were able to infect mice with little or no pathological symptoms, and both reduced the pathology in mice later challenged with the parental wild-type disease-causing Chlamydia strain. Thus, plasmid-deficient Chlamydia are effective vaccine strains. The invention provides a process for isolating a plasmid-deficient strain of Chlamydia sp., a process for developing a plasmid-deficient strain of Chlamydia sp. for use as a vaccine, a process for developing a highly infective plasmid-deficient strain of Chlamydia sp., isolated Chlamydia sp. strains, a method of expressing a recombinant nucleic acid in Chlamydia sp., and a method of immunizing a mammal against a Chlamydia infection.

摘要翻译： 本发明涉及发现衣原体属 菌株可以通过用新生霉素处理来固化其质粒，并且质粒缺陷菌株在标准条件下感染细胞是有缺陷的，但如果离心到宿主细胞上则可感染细胞。 但是发现在标准条件下具有野生型感染效率的质粒缺陷株可以通过在标准条件下选择感染来分离具有低感染性的亲本质粒缺陷型菌株的突变体。 感染性和感染性较差的质粒缺失型菌株能够感染具有很少或没有病理学症状的小鼠，并且都减少随后用亲本野生型致病衣原体菌株攻击的小鼠的病理学。 因此，质粒缺陷型衣原体是有效的疫苗株。 本发明提供了一种用于分离衣原体属质粒缺陷菌株的方法，该方法是开发沙眼衣原体质粒缺陷型菌株的方法。 用作疫苗，开发高度感染性质粒缺失型衣原体菌株的方法，分离的衣原体属。 菌株，在衣原体中表达重组核酸的方法，以及免疫哺乳动物抵抗衣原体感染的方法。

公开(公告)号：US07011824B2

公开(公告)日：2006-03-14

申请号：US09729579

申请日：2000-12-04

申请人： Philip A. Moore, Jr.

发明人： Philip A. Moore, Jr.

IPC分类号： A61L11/00

CPC分类号： C02F11/14 ,  C02F2103/20

摘要： A method of treating animal manure solids comprises contacting the solids with a treatment composition comprising AlCl3.nH2O or Al(NO3)3.mH2O, or the residue of AlCl3.nH2O or Al(NO3)3.mH2O, to form a treated waste product, wherein n is from 0 to 10, and m is from 0 to 12. The treatment amount can be effective to reduce phosphorus solubility in the manure; reduce phosphorus runoff and/or phosphorus leaching from fields fertilized with manure; inhibit ammonia volatilization from the manure; flocculate solids in the manure; reduce pathogens in the manure; increase the nitrogen content in the manure; and/or reduce acid rain and PM-10s associated with the manure.

摘要翻译： 一种处理动物粪便固体的方法包括使固体与包含AlCl 3·2H 2 O或Al（NO 3 3）O的处理组合物接触， 或者是AlCl 3 N，O 2 O或Al（NO 3）2的残基， 3 3 2H，以形成经处理的废物，其中n为0至10，m为0至12。 处理量可以有效减少粪便中磷的溶解度; 减少用肥肥施肥的田地磷磷径流和/或磷浸出; 抑制粪便中的氨挥发; 絮凝粪便中的固体; 减少粪便中的病原体; 增加粪便中的氮含量; 和/或减少与粪便相关的酸雨和PM-10。

公开(公告)号：US20040016094A1

公开(公告)日：2004-01-29

申请号：US10340806

申请日：2003-01-13

申请人： The Board of Trustees of the University of Arkansas

发明人： Leonard W. Schaper

IPC分类号： H01G007/00 ,  H05K003/20

CPC分类号： H01G4/35 ,  H01G4/385 ,  H01L2224/48091 ,  H01L2224/48227 ,  H01L2924/01068 ,  H01L2924/01079 ,  H01L2924/01087 ,  H01L2924/09701 ,  H01L2924/15153 ,  H01L2924/1517 ,  H01L2924/15311 ,  H01L2924/1532 ,  H01L2924/16195 ,  H01L2924/19041 ,  H01L2924/30107 ,  H01L2924/3011 ,  Y10T29/43 ,  Y10T29/435 ,  Y10T29/49128 ,  Y10T29/4913 ,  H01L2924/00014

摘要： A method of forming a capacitor comprises: providing a first metallic thin-film layer; forming a dielectric thin-film layer of an anodized metal on a side of said first metallic layer; providing a second metallic thin-film layer on a side of the dielectric layer opposite the first metallic layer; and excising an area of the resulting structure to obtain at least one capacitor having a pair of electrode plate structures constituted by portions of said first and second metallic layers separated by a portion of said dielectric layer, and having connection points at adjacent electrode plate edges which are accessible from a same side of the capacitor and effective for operatively connecting the capacitor such that the capacitor does not exhibit an inductive resonance below a frequency of at least 1 GHz.

摘要翻译： 一种形成电容器的方法包括：提供第一金属薄膜层; 在所述第一金属层的一侧上形成阳极氧化金属的电介质薄膜层; 在与所述第一金属层相对的所述电介质层的一侧上设置第二金属薄膜层; 并且切除所得结构的区域以获得至少一个电容器，该电容器具有由所述第一和第二金属层的部分由所述电介质层的一部分隔开的部分构成的电极板结构，并且在邻近的电极板边缘处具有连接点 可以从电容器的同一侧进入，并有效地可操作地连接电容器，使得电容器在低至少1GHz的频率下不会出现电感谐振。

公开(公告)号：US6125685A

公开(公告)日：2000-10-03

申请号：US163945

申请日：1998-09-30

申请人： Rebecca E. Collier ,  Mark L. Kuss

发明人： Rebecca E. Collier ,  Mark L. Kuss

IPC分类号： G01M99/00 ,  G01N3/56 ,  G01N33/42 ,  E01C23/01 ,  G01L5/00 ,  G01M19/00

CPC分类号： G01N3/56 ,  G01N2203/0071 ,  G01N33/42

摘要： The present invention provides and apparatus and method for analyzing the rutting and stripping characteristics of hot mix asphalt concrete samples. The apparatus of the invention measures the vertical penetration of a wheel rolling in a reciprocating motion across the surface of a compacted asphalt mix specimen. The apparatus incorporates precise environmental control and includes features for testing two or more samples simultaneously under wet or dry conditions or a combination of wet and dry conditions.

摘要翻译： 本发明提供了用于分析热混沥青混凝土样品的车辙和剥离特性的装置和方法。 本发明的装置测量了经过压实的沥青混合物样品的往复运动的滚轮的垂直穿透。 该设备结合精确的环境控制，并且包括在湿或干燥条件下或湿和干燥条件的组合下同时测试两个或更多个样品的特征。

公开(公告)号：US6023408A

公开(公告)日：2000-02-08

申请号：US630030

申请日：1996-04-09

申请人： Leonard W. Schaper

发明人： Leonard W. Schaper

IPC分类号： H01G4/33 ,  H01G4/00 ,  H01G4/005 ,  H01G4/008 ,  H01G4/012 ,  H01G4/06 ,  H01G4/20 ,  H01G4/228 ,  H01G4/35 ,  H01G4/38 ,  H01G7/00 ,  H05K3/20

CPC分类号： H01G4/35 ,  H01G4/385 ,  H01L2224/48091 ,  H01L2224/48227 ,  H01L2924/01068 ,  H01L2924/01079 ,  H01L2924/01087 ,  H01L2924/09701 ,  H01L2924/15153 ,  H01L2924/1517 ,  H01L2924/15311 ,  H01L2924/1532 ,  H01L2924/16195 ,  H01L2924/19041 ,  H01L2924/30107 ,  H01L2924/3011 ,  Y10T29/43 ,  Y10T29/435 ,  Y10T29/49128 ,  Y10T29/4913

摘要： A capacitor having a floating plate-shaped electrode, at least two patterned plate electrodes overlying the floating plate-shaped electrode, and a dielectric layer therebetween. The resulting structure exhibits high two-port insertion loss even at frequencies as high as 10 GHz. Notably, the capacitor exhibits an insertion loss of more than -40 dB over a range from 1 GHz to 10 GHz.

摘要翻译： 具有浮置板状电极的电容器，覆盖浮置板状电极的至少两个图案化的板状电极以及它们之间的介电层。 所得到的结构即使在高达10GHz的频率也表现出高双端口插入损耗。 值得注意的是，电容器在1 GHz至10 GHz的范围内表现出大于-40 dB的插入损耗。

公开(公告)号：US5976818A

公开(公告)日：1999-11-02

申请号：US626675

申请日：1996-04-02

申请人： Timothy J. O'Brien

发明人： Timothy J. O'Brien

IPC分类号： C07K16/30 ,  G01N33/574 ,  G01N33/48 ,  G01N33/53 ,  G01N33/542

CPC分类号： C07K16/3069 ,  G01N33/57449

摘要： Hybridomally produced monoclonal antibodies specifically immunoreactive with the glycoprotein carrying the CA 125 epitope. Monoclonal antibodies recognize both high and low molecular weight subunits of the antigen, and identify the antigen in the cytoplasm and the extracellular matrix of CA 125 producing cells. An immunoassay for the detection of CA 125 utilizing the monoclonal antibodies is described.

摘要翻译： 与携带CA125表位的糖蛋白特异性免疫反应的杂交产生的单克隆抗体。 单克隆抗体识别抗原的高分子量和低分子量亚基，并鉴定CA 125产生细胞的细胞质和细胞外基质中的抗原。 描述了利用单克隆抗体检测CA125的免疫测定。