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    Methods and Compositions for PCR Using Blocked and Universal Primers
    61.
    发明申请
    Methods and Compositions for PCR Using Blocked and Universal Primers 有权
    使用封闭和通用引物进行PCR的方法和组合

    公开(公告)号：US20140329245A1

    公开(公告)日：2014-11-06

    申请号：US14154117

    申请日：2014-01-13

    Applicant: UniTaq Bio

    Inventor： Eugene Spier ,  Karl Guegler

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q2525/15 ,  C12Q2537/143 ,  C12Q2537/163 ,  C12Q2521/301 ,  C12Q2521/327 ,  C12Q2525/161 ,  C12Q2525/186 ,  C12Q2547/101

    Abstract: Provided herein are methods and compositions for performing PCR with primers with blocked 3′-ends that are unblocked when these primers anneal to the template. The multiplexed PCR can be used as real-time qPCR, for end-point detection or as enrichment method for next generation sequencing (NGS). Also described herein are methods and compositions to improve sensitivity of mutation-specific PCR when targeting closely-spaced mutations.

    Abstract translation: 本文提供了当这些引物与模板退火时，用具有封闭的3'末端的引物进行PCR的方法和组合物，其被解封。 复用PCR可用作实时qPCR，用于终点检测或用于下一代测序（NGS）的浓缩方法。 本文还描述了当靶向紧密间隔的突变时提高突变特异性PCR的灵敏度的方法和组合物。

    EFFICIENT BASE DETERMINATION IN SEQUENCING REACTIONS
    62.
    发明申请
    EFFICIENT BASE DETERMINATION IN SEQUENCING REACTIONS 审中-公开
    顺序反应中有效的碱基测定

    公开(公告)号：US20140213461A1

    公开(公告)日：2014-07-31

    申请号：US13965166

    申请日：2013-08-12

    Applicant: Complete Genomics, Inc.

    Inventor： Radoje Drmanac ,  Matthew J. Callow

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6855 ,  C12Q2531/125 ,  C12Q2521/313 ,  C12Q2521/125 ,  C12Q2537/163 ,  C12Q2525/191 ,  C12Q2521/531 ,  C12Q2521/501 ,  C12Q2525/307 ,  C12Q2525/151

    Abstract: The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.

    Abstract translation: 本发明涉及用于核酸鉴定和检测的组合物和方法。 本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。 本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。

    Detection of methicillin-resistant Staphylococcus aureus
    66.
    发明授权
    Detection of methicillin-resistant Staphylococcus aureus 有权
    检测耐甲氧西林金黄色葡萄球菌

    公开(公告)号：US08206921B2

    公开(公告)日：2012-06-26

    申请号：US12620187

    申请日：2009-11-17

    Applicant: Henrik Stender ,  Anne Karin Ildor Rasmussen ,  Mark J. Fiandaca

    Inventor： Henrik Stender ,  Anne Karin Ildor Rasmussen ,  Mark J. Fiandaca

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/689 ,  C12Q1/6837 ,  C12Q2537/125 ,  C12Q2537/163 ,  C12Q2525/113

    Abstract: This invention relates to the use of genetic probes for detection of the presence of the SCCmec cassette in Staphylococcus aureus. In one aspect, the invention allows specific detection and identification of methicillin-resistant S. aureus (MRSA) in a clinical sample without interference from the presence of other non-S. aureus methicillin-resistant staphylococci. In another aspect, the invention allows specific detection and identification of methicillin-resistant coagulase negative staphylococci (MRCNS) originating from a clinical sample without interference from the presence of methicillin-resistant S. aureus.

    Abstract translation: 本发明涉及使用遗传探针来检测金黄色葡萄球菌中SCCmec盒的存在。 一方面，本发明允许在临床样品中特异性检测和鉴定耐甲氧西林金黄色葡萄球菌（MRSA），而不存在来自其他非S的存在的干扰。 金黄色葡萄球菌耐药葡萄球菌。 在另一方面，本发明允许特异性检测和鉴定来自临床样品的耐甲氧西林凝固酶阴性葡萄球菌（MRCNS），而不受来自耐甲氧西林金黄色葡萄球菌存在的干扰。

    PROBE SET FOR IDENTIFICATION OF NUCLEOTIDE MUTATION, AND METHOD FOR IDENTIFICATION OF NUCLEOTIDE MUTATION
    68.
    发明申请
    PROBE SET FOR IDENTIFICATION OF NUCLEOTIDE MUTATION, AND METHOD FOR IDENTIFICATION OF NUCLEOTIDE MUTATION 审中-公开
    用于鉴定核苷酸突变的探针组和用于鉴定核苷酸突变的方法

    公开(公告)号：US20120095116A1

    公开(公告)日：2012-04-19

    申请号：US13278288

    申请日：2011-10-21

    Applicant: Yasuhiro KISHI ,  Naohiro Kamiya

    Inventor： Yasuhiro KISHI ,  Naohiro Kamiya

    IPC: C12Q1/70 ,  A61K35/00 ,  A61P31/14 ,  C12Q1/68

    CPC classification number: C12Q1/6825 ,  C12N9/506 ,  C12Q1/6858 ,  C12Q1/707 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Y304/21098 ,  C12Q2561/113 ,  C12Q2525/113 ,  C12Q2535/131 ,  C12Q2537/163 ,  C12Q2561/101

    Abstract: A probe set comprising a detection probe and a counter probe, wherein the detection probe comprises an oligonucleotide which comprises a nucleotide sequence containing the mutation site on the target nucleic acid and also containing a nucleotide of interest in the mutation site or a nucleotide sequence complementary to the aforementioned nucleotide sequence, has a fluorescent substance added to the 5′-terminal and a quenching substance added to the 3′-terminal, and has, introduced therein, such a modification that the melting temperature of the probe becomes 3° C. or more higher than that of the counter probe, and wherein the counter probe comprises an oligonucleotide which comprises a nucleotide sequence containing a mutation site and also containing a nucleotide that is different from the nucleotide of interest in the mutation site or a nucleotide sequence complementary to the aforementioned nucleotide sequence.

    Abstract translation: 一种包含检测探针和对位探针的探针组，其中所述检测探针包含寡核苷酸，其包含在靶核酸上含有突变位点并且还含有所述突变位点的目标核苷酸的核苷酸序列或与 上述核苷酸序列具有添加到5'末端的荧光物质和添加到3'-末端的淬灭物质，并且在其中引入了使探针的熔融温度变为3℃的修饰，或 比对照探针更高，并且其中所述反探针包含寡核苷酸，其包含含有突变位点的核苷酸序列，并且还含有与所述突变位点中感兴趣的核苷酸不同的核苷酸或与所述突变位点互补的核苷酸序列 上述核苷酸序列。

    COMPOSITIONS FOR DETECTING HUMAN INTERFERON-ALPHA SUBTYPES AND METHODS OF USE
    69.
    发明申请
    COMPOSITIONS FOR DETECTING HUMAN INTERFERON-ALPHA SUBTYPES AND METHODS OF USE 有权
    用于检测人类干扰素ALPHA亚型的组合物及其使用方法

    公开(公告)号：US20110311973A1

    公开(公告)日：2011-12-22

    申请号：US13130346

    申请日：2009-11-20

    Applicant: Ronald Rabin ,  Viraj Pramod Mane

    Inventor： Ronald Rabin ,  Viraj Pramod Mane

    IPC: C12Q1/68 ,  C07H1/00

    CPC classification number: C12Q1/6883 ,  C12Q1/6858 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2537/143 ,  C12Q2535/125 ,  C12Q2537/163 ,  C12Q2535/131

    Abstract: The invention provides highly sensitive, specific and efficient quantitative real-time PCR compositions, methods and assay kits to detect at least one IFN subtype and/or IFN subtype allotypic variants. Primer/probe sets complementary to the coding sequence of an IFN subtype of interest avoid spurious detection of degraded mRNA and enhances the correlation between the IFN subtype that is measured by the assays of the invention and the protein that is actually expressed. The invention also provides methods for designing primers and methods of using the compositions and assay kits. The compositions, kits, and methods of the invention may be used, for example, to monitor vaccine efficacy, autoimmune disease, chronic infections, or tumor therapy.

    Abstract translation: 本发明提供高灵敏度，特异性和有效的定量实时PCR组合物，方法和测定试剂盒，以检测至少一种IFN亚型和/或IFN亚型异型变体。 与感兴趣的IFN亚型的编码序列互补的引物/探针组避免了降解mRNA的杂散检测，并增强了通过本发明的测定法和实际表达的蛋白质测量的IFN亚型之间的相关性。 本发明还提供了设计引物的方法和使用组合物和测定试剂盒的方法。 本发明的组合物，试剂盒和方法可用于例如监测疫苗功效，自身免疫疾病，慢性感染或肿瘤治疗。

    Methods for molecular detection
    70.
    发明授权
    Methods for molecular detection 有权
    分子检测方法

    公开(公告)号：US08034569B2

    公开(公告)日：2011-10-11

    申请号：US12479806

    申请日：2009-06-06

    Applicant: George Jackson ,  Christopher Quan

    Inventor： George Jackson ,  Christopher Quan

    IPC: C12Q1/68

    CPC classification number: C12Q1/6804 ,  C12Q1/6844 ,  C12Q2525/205 ,  C12Q2537/161 ,  G01N33/5308 ,  C12Q2537/163 ,  C12Q2525/207

    Abstract: This invention relates to methods for molecular detection, particularly to methods utilizing target-specific molecular probes. In exemplary embodiments, target-specific molecular probes include single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) aptamers. In general, the molecular probe may bind with relatively high specificity to a given target. In one aspect, a method for molecular detection comprises a molecular probe paired to a reporter molecule wherein the molecular probe impairs the amplification of the reporter molecule in the absence of the target molecule.

    Abstract translation: 本发明涉及分子检测方法，特别涉及利用靶特异性分子探针的方法。 在示例性实施方案中，靶特异性分子探针包括单链脱氧核糖核酸（DNA）或核糖核酸（RNA）适体。 通常，分子探针可以以相对高的特异性结合给定的靶。 在一个方面，用于分子检测的方法包括与报告分子配对的分子探针，其中分子探针在不存在靶分子的情况下损害了报告分子的扩增。

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