公开(公告)号：US20040157285A1

公开(公告)日：2004-08-12

申请号：US10478785

申请日：2003-11-24

发明人： Kaori Ishimaru ,  Satoshi Yonehara

IPC分类号： G01N001/30 ,  G01N033/48 ,  C07D257/02

CPC分类号： C07D257/04 ,  C07B63/04

摘要： A method for storing a tetrazolium compound stably is provided. The tetrazolium compound is stored in the presence of sodium azide. The tetrazolium compound (A) and the sodium azide (B) are present at a ratio (A:B) in the range from 1:0.02 to 1:6.2. Furthermore, when the tetrazolium compound is stored as a solution, the concentration of the sodium azide is in the range from 0.08 to 3.2 mmol/L and the concentration of the tetrazolium compound is in the range from 0.5 to 8 mmol/L. As the tetrazolium compound, it is preferable to use 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt.

摘要翻译： 提供稳定地存储四唑化合物的方法。 四唑化合物在叠氮化钠存在下储存。 四氮唑化合物（A）和叠氮化钠（B）的比例（A:B）为1:0.02至1:6.2。 此外，当四唑鎓化合物作为溶液储存时，叠氮化钠的浓度在0.08-3.2mmol / L的范围内，四唑鎓化合物的浓度在0.5-8mmol / L的范围内。 作为四唑鎓化合物，优选使用2-（4-碘苯基）-3-（2,4-二硝基苯基）-5-（2,4-二磺基苯基）-2H-四唑鎓盐。

公开(公告)号：US20040132128A1

公开(公告)日：2004-07-08

申请号：US10686674

申请日：2003-10-17

申请人： The Provost, Fellows and Scholars of the College of the Holy ,  Undivided Trinity of Queen Elizabeth near Dublin

发明人： Igor Shvets ,  Dmitriy Kashanin ,  Dermot Kelleher ,  Vivienne Williams ,  Yuri Volkov

IPC分类号： G01N001/30

CPC分类号： G01N33/5005 ,  B01L3/5027 ,  B01L3/502761 ,  B01L3/5635 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/163 ,  B01L2400/0487

摘要： Biological assays using various constructions of biochips are disclosed to mirror in vivo situations. The biochip 50 comprises a microchannel 51 having a liquid outlet port 1, bubble release port 2 and a liquid outlet port 3 with an associated bubble release port 4. A multiplicity of tests can be performed often by coating the bore of the microchannel 50 which various adhesion mediating proteins or the use of chemoattractants. The assay assembly 60 comprises a syringe pump feeding the biochip 50. An inverted microscope 65, digital camera 66 and recorder 67 are provided. A sample liquid containing cells in suspension is injected slowly through the biochip and the effect of the assay recorded over a long period.

摘要翻译： 公开了使用生物芯片的各种构造的生物测定来体现体内情况。 生物芯片50包括具有液体出口1，气泡释放口2和具有相关联的气泡释放口4的液体出口3的微通道51.可以经常通过涂覆微通道50的孔来进行多种测试， 粘附介导蛋白或使用化学引诱物。 测定组件60包括供给生物芯片50的注射泵。提供倒置显微镜65，数字照相机66和记录器67。 将含有悬浮液中的细胞的样品液体缓慢注入生物芯片，并且测定长时间的记录效果。

公开(公告)号：US20040132068A1

公开(公告)日：2004-07-08

申请号：US10686865

申请日：2003-10-16

发明人： Walter Schubert

IPC分类号： C12Q001/68 ,  G01N001/30 ,  G01N033/48 ,  C12N001/08

CPC分类号： B01L7/00 ,  B01L2400/049 ,  G01N1/30 ,  G01N1/312 ,  G01N2035/00366

摘要： The invention relates to a method for preparing biological samples for analysis, comprising the following steps: (a) the biological sample is placed on a two-dimensional support; (b) application of a protein-precipitating or denaturing first solution L1 to the biological sample at a first temperature T1 for a predetermined first time period Z1; (c) the protein-precipitating or denaturing first solution L1 is then left, or more solution is applied to the biological sample or a protein-precipitating or denaturing second solution L2 is applied to the biological sample at a temperature T2, for a predetermined second time period Z2, whereby T2 is lower than T1 and Z2 is longer, equal to or shorter than Z1; and (d) drying of the sample. The invention also relates to a device for carrying out a method for the preparation of biological samples for analysis, in accordance with one of the aforementioned claims, said device (10) comprising at least one chamber (12) for receiving the biological sample or samples that has/have been applied to at least one support and comprising at least one temperature controller (14) for controlling and adjusting the temperature inside the chamber (12).

摘要翻译： 本发明涉及一种制备用于分析的生物样品的方法，包括以下步骤：（a）将生物样品置于二维载体上; （b）将蛋白质沉淀或变性的第一溶液L1以第一温度T1施加到生物样品预定的第一时间段Z1; （c）然后留下蛋白沉淀或变性的第一溶液L1，或者将更多的溶液施加到生物样品上，或者将蛋白质沉淀或变性的第二溶液L2在温度T2下施加到生物样品上预定的秒 时间段Z2，由此T2低于T1，Z2长于等于或小于Z1; 和（d）样品的干燥。 本发明还涉及一种用于执行用于制备用于分析的生物样品以用于分析的方法的装置，所述装置（10）包括用于接收生物样品或样品的至少一个室（12） 其已被施加到至少一个支撑件并且包括用于控制和调节室（12）内的温度的至少一个温度控制器（14）。

公开(公告)号：US20040077073A1

公开(公告)日：2004-04-22

申请号：US10273462

申请日：2002-10-18

发明人： Melvin Schindler ,  John F. Holland ,  Max R. Olinger

IPC分类号： A61N001/30 ,  G01N001/30 ,  G01N033/48 ,  C12M001/34

CPC分类号： C12M47/04 ,  B01J2219/00441 ,  B01J2219/00495 ,  B01J2219/00527 ,  B01J2219/00934 ,  B01L3/508 ,  C40B60/14 ,  G01N35/00029 ,  G01N2001/284

摘要： An apparatus for micromanipulating biological materials, comprising: (a) a laser emitting light at a wavelengthnull600 nm; (b) a matrix supporting the biologic material, comprising a light-absorbing material; and (c) a system for focusing light from the source onto specific regions of the matrix. The light absorbing material absorbs the light and coverts it to heat so as to disrupt the matrix and the biological material at the point where the light contacts the matrix. Preferably, the matrix is supported by a carrier to form a bi-layer matrix composite. In another embodiment, the matrix is supported on a support plate having an aperture which is covered, at least in part, by the matrix. In another embodiment, the a matrix is supported by a carrier, wherein at least one of the matrix and the support plate comprises a cell growth modifier.

摘要翻译： 一种用于微生物生物材料的装置，包括：（a）发射波长> = 600nm的光的激光; （b）支撑生物材料的基质，包含光吸收材料; 和（c）用于将来自源的光聚焦到矩阵的特定区域的系统。 光吸收材料吸收光并将其覆盖以加热，以便在光接触基质的点处破坏基质和生物材料。 优选地，基质由载体负载以形成双层基质复合材料。 在另一个实施例中，矩阵被支撑在具有至少部分被矩阵覆盖的孔的支撑板上。 在另一个实施方案中，所述基质由载体支持，其中所述基质和所述支持板中的至少一个包含细胞生长调节剂。

公开(公告)号：US20040053338A1

公开(公告)日：2004-03-18

申请号：US10350558

申请日：2003-01-24

发明人： Yogendra Sharma ,  Bheemreddy Rajini Devi

IPC分类号： G01N033/53 ,  G01N033/567 ,  G01N001/30 ,  G01N033/48

CPC分类号： G01N33/6893 ,  G01N2800/166

摘要： The present invention relates to a method of identifying calcium binding sites in gamma-crystallin useful in calcium-based homeostasis for the management of Cataract, said method comprising steps of isolating gamma-crystallin from eye lens, studying binding of calcium to the isolated protein by both direct and indirect methods, identifying Greek key Motif as calcium-binding sites of the protein gamma crystallin with the said protein binding 4-mol eq of calcium having dissociation constant of 90micromole.

摘要翻译： 本发明涉及一种鉴定用于治疗白内障的基于钙的体内平衡的γ-晶状蛋白中的钙结合位点的方法，所述方法包括从眼晶状体中分离γ-晶状蛋白的步骤，通过以下步骤研究钙与分离的蛋白质的结合： 直接和间接方法，鉴定希腊关键基序作为蛋白质γ-晶状蛋白的钙结合位点，所述蛋白质结合4摩尔当量的解离常数为90微摩尔的钙。

公开(公告)号：US20040048330A1

公开(公告)日：2004-03-11

申请号：US10332303

申请日：2003-06-26

发明人： Christoph Bittner

IPC分类号： G01N001/30 ,  G01N033/48 ,  G06K009/00

CPC分类号： C12M41/36 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/1493

摘要： The invention relates to a method for the examination of cells (20, 38) in a culture medium, in particular for in-situ microscopy in a bio-reactor, whereby cells in a sample volume, the depth (d) of which is defined by windows (14, 16) in the direction of the optical axis of the microscope (18), are microscopically imaged and are automatically recorded and processed by means of an image processing system (30). Said method is characterized in that the depth (d) of the sample volume (12) is adjusted to the size of the cells (20, 38) by successively reducing the separation (d) of the windows while the image size (G) of the cells is simultaneously verified by the image processing system (30) such that a separation value (D) is determined at which the image size (G) of the cells begins to grow, thus corresponding to flattening caused by the contact pressure of the windows (14, 16), and that the separation (d) of the windows (14, 16) is set to said separation value (D) for the examination.

摘要翻译： 本发明涉及一种用于检查培养基中细胞（20,38）的方法，特别是用于在生物反应器中进行原位显微镜检查的方法，其中样品体积中的细胞（其深度（d）被限定） 通过窗口（14,16）在显微镜（18）的光轴方向上进行微观成像，并通过图像处理系统（30）自动记录和处理。 所述方法的特征在于，通过连续地减小窗口的分离（d），将样本体积（12）的深度（d）调整到单元格（20,38）的大小，同时图像尺寸（G） 通过图像处理系统（30）同时验证单元，使得确定单元的图像尺寸（G）开始增长的分离值（D），从而对应于由窗口的接触压力引起的平坦化 （14,16），并且窗口（14,16）的分离（d）被设置为用于检查的所述分离值（D）。

公开(公告)号：US20040023320A1

公开(公告)日：2004-02-05

申请号：US10415179

申请日：2003-04-24

发明人： Georg E. Steiner ,  Rupert Ch. Ecker

IPC分类号： G06F019/00 ,  G01N033/48 ,  G01N033/50 ,  G01N001/30 ,  G06K009/00

CPC分类号： G01N15/1475 ,  G01N2015/1006 ,  G01N2015/1472 ,  G01N2015/1486 ,  G06K9/0014

摘要： The invention relates to a method for analyzing cells that are present as closed clusters. According to said method, a planar tissue preparation is subjected to an identification staining of the cell nuclei and a target structure staining of cell objects that is different from the identification staining. Digital images are recorded of the stained tissue preparation by means of an electronic image recording device and at least one image of a subsection of the tissue cut is displayed in at least one coloration. According to the inventive method, at least one parameter of the cell nuclei and at least one parameter of the cell objects labeled by target structure staining is restricted to a predetermined range of values. Cell nuclei and cell objects whose parameters correspond to the respective parameter range(s) are detected and optionally displayed using image processing algorithms in the image of said subsection. The image content of at least one image detected for the cell nuclei is correlated with the image content of at least one image detected for the target-structure stained cell objects to detect the individual cells. On the basis of the cell nuclei identified a cell growth or a cell enlargement is induced using a predetermined arithmetic algorithm to reconstruct the individual cells. In doing so it is made sure that neighboring cells do not fuse. The number of reconstructed individual cells is determined and/or the individual cells are divided into populations according to certain parameters.

摘要翻译： 本发明涉及一种用于分析作为封闭簇存在的细胞的方法。 根据所述方法，对平面组织制剂进行细胞核的鉴定染色和不同于鉴定染色的细胞对象的靶结构染色。 通过电子图像记录装置记录染色的组织制剂的数字图像，并且以至少一种着色显示组织切割的子部分的至少一个图像。 根据本发明的方法，将细胞核的至少一个参数和由目标结构染色标记的细胞对象的至少一个参数限制在预定的值范围内。 其参数对应于相应的参数范围的单元核和单元对象被检测并且可选地使用所述子部分的图像中的图像处理算法来显示。 对于细胞核检测到的至少一个图像的图像内容与针对目标结构染色的细胞对象检测到的至少一个图像的图像内容相关，以检测各个细胞。 在细胞核的基础上，使用预定的算术算法诱导细胞生长或细胞增殖以重建各个细胞。 在这样做时，确保相邻的电池不熔断。 根据某些参数确定重建的个体细胞的数量和/或个体细胞被分成群体。

公开(公告)号：US20040018247A1

公开(公告)日：2004-01-29

申请号：US10208516

申请日：2002-07-29

发明人： Walter Luis Wolmeister

IPC分类号： G01N001/30 ,  G01N033/48 ,  A61K033/24

CPC分类号： C14C3/06

摘要： FORMULATION AND THE RESPECTIVE PRESERVATION PROCESS OF ANIMAL AND VEGETABLE TISSUES, presented in liquid form and that has the particularity of having low basicity and a masking and complexing balanced system, where such characteristics grant to the product a high power of diffusion and distribution in the most internal regions of the hide structure, guaranteeing a fast transformation, and producing leathers with special properties, being able to be used in the processing of any type of hide, and being particularly indicated for bovine leathers of greater thickness (integral), where with the commonly used formulations a rather longer processing time is needed.

摘要翻译： 动物和蔬菜组织的配方和相关保存程序以液体形式呈现，具有低碱性和掩蔽和络合平衡系统的特殊性，其中这种特性给予产品最大的扩散和分布功能 皮革结构的内部区域，保证快速转型，并生产具有特殊性能的皮革，能够用于任何类型的皮革的加工，特别适用于厚度较大（整体）的牛皮，其中 常用的配方需要相当长的处理时间。

公开(公告)号：US20040009545A1

公开(公告)日：2004-01-15

申请号：US10349396

申请日：2003-01-21

申请人： Caliper Technologies Corp.

发明人： Javier Anibal Farinas ,  H. Garrett Wada

IPC分类号： C12Q001/02 ,  G01N001/30 ,  G01N033/48 ,  C12M001/34

CPC分类号： G01N31/22 ,  G01N1/30 ,  G01N33/5005 ,  G01N2001/302 ,  G01N2458/00 ,  Y10S435/968

摘要： Transmembrane potential measurement methods using cationic dyes, and anionic dyes are provided. Compositions of the cationic and anionic dyes and microfluidic systems which include the dyes and membranes are provided in conjunction with processing elements for transmembrane potential measurements.

公开(公告)号：US20030186248A1

公开(公告)日：2003-10-02

申请号：US10109953

申请日：2002-03-29

发明人： Mark G. Erlander ,  Dennis C. Sgroi ,  Xiao-Jun Ma ,  Thomas M. Baer

IPC分类号： C12Q001/68 ,  G01N001/30 ,  G01N033/48

CPC分类号： C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/158 ,  G01N33/5091

摘要： The present invention relates to the use of molecular histological signatures to interpret and correlate cytological specimens with the presence or absence of disease and the progression thereof. The invention provides molecular signatures for use in the study and/or diagnosis of diseased cells and tissues of a cytological specimen relative to a solid (e.g. histological) sample.

摘要翻译： 本发明涉及分子组织学特征用于解释和关联细胞学标本与存在或不存在疾病及其进展的用途。 本发明提供用于研究和/或诊断相对于固体（例如组织学）样品的细胞学标本的病变细胞和组织的分子特征。