公开(公告)号：US20160169922A1

公开(公告)日：2016-06-16

申请号：US14948938

申请日：2015-11-23

Applicant: Wake Forest University Health Sciences

Inventor： Lawrence L. Rudel ,  Chadwick D. Miller ,  Michael J. Thomas

IPC: G01N33/92 ,  G06F19/00

CPC classification number: G01N33/92 ,  G01N2405/00 ,  G01N2560/00 ,  G01N2800/323 ,  G01N2800/50 ,  G01N2800/56 ,  G06F19/00 ,  G16H50/30

Abstract: A computer-based method for determining a prediction of risk and/or an indication of extent of coronary stenosis in a human subject, comprises the steps of: (a) inputting the level of at least one cholesteryl ester measured in a blood sample collected from said subject; and then (b) inputting the age and gender of said subject; and then (c) generating in said computer from said cholesteryl ester level input, said age input and said gender input a prediction of risk and/or an indication of extent of coronary stenosis in said subject. Systems and methods are also described.

Abstract translation: 一种用于确定人类受试者的风险预测和/或冠状动脉狭窄程度的指示的基于计算机的方法包括以下步骤：（a）输入在收集的血液样本中测量的至少一种胆固醇酯的水平 主题; 然后（b）输入所述受试者的年龄和性别; 然后（c）在所述计算机中从所述胆固醇酯水平输入，所述年龄输入和所述性别输入产生所述受试者的风险预测和/或冠状动脉狭窄程度的指示。 还描述了系统和方法。

公开(公告)号：US20160166772A1

公开(公告)日：2016-06-16

申请号：US14966326

申请日：2015-12-11

Applicant: Wake Forest University Health Sciences

Inventor： Majid Mirzazadeh ,  Philip J. Brown ,  Kenneth W. Russell

IPC: A61M5/315

CPC classification number: A61M5/3157 ,  A61M5/31513 ,  A61M5/31526 ,  A61M5/31573 ,  A61M5/31595 ,  A61M2005/31508 ,  A61M2205/581 ,  A61M2205/582

Abstract: An incremental syringe useful for multiple injections of medications like botulinum toxin is provided. The syringe includes detents on the syringe plunger which provide a tactile feeling, a discrete audible sound or “click,” or preferably both, for every unit of medication aspirated or injected to or from an individual syringe. Hence, there is no need to look at the syringe, or bring it to the eye level, during use thereof. In some embodiments, a second set of detents is included, and in some embodiments a third set of detents is included. Syringe plungers useful for combining with a syringe body to produce such an incremental syringe are also described.

Abstract translation: 提供了可用于多次注射药物如肉毒杆菌毒素的增量注射器。 注射器包括注射器柱塞上的棘爪，其针对从单个注射器吸取或注射的每个单位的药物提供触觉，离散的声音或“点击”，或者优选地两者。 因此，在使用期间不需要观察注射器，或将其带到眼睛水平。 在一些实施例中，包括第二组制动器，并且在一些实施例中包括第三组制动器。 还描述了可用于与注射器主体结合以制造这种增量注射器的注射器柱塞。

公开(公告)号：US20160145619A1

公开(公告)日：2016-05-26

申请号：US15010615

申请日：2016-01-29

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： William H. Gmeiner

IPC: C12N15/115 ,  A61K47/48 ,  A61K31/713

CPC classification number: C12N15/115 ,  A61K31/713 ,  A61K47/549 ,  C12N2310/16 ,  C12N2310/3519 ,  C12N2320/30 ,  Y02A50/402

Abstract: A compound of the formula A-B-C, is provided, wherein: A is a first nucleic acid that specifically binds to an extracellular surface protein expressed by a cell of interest, B is an alkyl linker; and C is a second nucleic acid that hybridizes to a complementary nucleic acid. In some embodiments, the first nucleic acid is an aptamer. In some embodiments, the nucleic acid comprises an active compound, particularly cytotoxic nucleotides such as poly-FdUMP. Compositions and methods of using such compounds for treating and/or detecting cancer are also described.

Abstract translation: 提供式A-B-C的化合物，其中：A是特异性结合由感兴趣细胞表达的细胞外表面蛋白质的第一核酸，B是烷基接头; C是与互补核酸杂交的第二个核酸。 在一些实施方案中，第一核酸是适体。 在一些实施方案中，核酸包含活性化合物，特别是细胞毒性核苷酸，例如poly-FdUMP。 还描述了使用这些化合物治疗和/或检测癌症的组合物和方法。

公开(公告)号：US20160089139A1

公开(公告)日：2016-03-31

申请号：US14869303

申请日：2015-09-29

Applicant: Wake Forest University Health Sciences

Inventor： L. Andrew Koman ,  Thomas L. Smith

IPC: A61B17/06

CPC classification number: A61B17/0493 ,  A61B17/06133 ,  A61B2017/06142 ,  A61B2017/06147 ,  A61B2017/2837 ,  A61B2090/0801

Abstract: Provided herein are protective devices that can fully capture and enclose a needle for a medical procedure, e.g., a swedged suture needle, such that the needle may safely remain attached to a wound or surgical site via suture material but also prevent accidental needle-stick injuries to medical personnel and patients. Such devices may be employed for temporary or longer periods of time while other phases related to the medical procedure transpire.

Abstract translation: 本文提供了能够完全捕获和封闭用于医疗过程的针的保护装置，例如，楔形缝合针，使得针可以通过缝合材料安全地保持附接到伤口或手术部位，但也可以防止意外的针刺损伤 给医务人员和病人。 这样的装置可以用于临时或更长的时间段，而与医疗程序相关的其他阶段也会发生。

公开(公告)号：US20160019692A1

公开(公告)日：2016-01-21

申请号：US14775271

申请日：2014-03-05

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Youngkyoo Jung

IPC: G06T7/00 ,  A61B5/026 ,  A61B5/00 ,  G06T7/40 ,  G06T11/00

CPC classification number: G06T7/0012 ,  A61B5/0042 ,  A61B5/0263 ,  A61B5/743 ,  A61B5/7445 ,  G06T7/155 ,  G06T7/90 ,  G06T11/008 ,  G06T2207/10081 ,  G06T2207/10088 ,  G06T2207/30016 ,  G06T2207/30101

Abstract: Methods, systems, computer programs, circuits and workstations are configured to generate at least one two-dimensional weighted CBF territory map of color-coded source artery locations using an automated vascular segmentation process to identify source locations using mutual connectivity in both image and label space.

Abstract translation: 方法，系统，计算机程序，电路和工作站被配置为使用自动血管分割过程来生成颜色编码的源动脉位置的至少一个二维加权CBF区域图，以使用图像和标签空间中的相互连接来识别源位置 。

公开(公告)号：US20150247194A1

公开(公告)日：2015-09-03

申请号：US14712395

申请日：2015-05-14

Applicant: Wake Forest University Health Sciences

Inventor： Jason Hipp ,  Shay Soker

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The present invention concerns methods of screening cells for differentiation or de-differentiation, and/or for status as a pluripotent or multipotent (e.g., “stem”) cell, by detecting the differential expression (e.g., upregulation, downregulation) of genes.

Abstract translation: 本发明涉及通过检测基因的差异表达（例如上调，下调）筛选用于分化或去分化的细胞和/或作为多潜能或多能（例如“干”）细胞的状态的方法。

公开(公告)号：US20150238656A1

公开(公告)日：2015-08-27

申请号：US14610902

申请日：2015-01-30

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Giuseppe Orlando ,  Shay Soker

IPC: A61L27/36 ,  A61L27/54 ,  A61L27/38

CPC classification number: A61L27/3633 ,  A61L27/3604 ,  A61L27/3687 ,  A61L27/3808 ,  A61L27/3834 ,  A61L27/54 ,  A61L2300/256 ,  A61L2300/42 ,  A61L2430/26 ,  A61L2430/28 ,  A61L2430/40

Abstract: Methods for decellularizing organs and tissues in vitro and in vivo are provided, as are methods of maintaining organ and tissue frameworks and methods of recellularizing organs and tissues, thereby providing an approach to needed organs or tissues.

Abstract translation: 提供体外和体内脱细胞化器官和组织的方法，以及维持器官和组织框架的方法以及使器官和组织接收的方法，从而提供所需器官或组织的方法。

公开(公告)号：US09039782B2

公开(公告)日：2015-05-26

申请号：US14256643

申请日：2014-04-18

Applicant: Wake Forest University Health Sciences

Inventor： Anthony Atala ,  James J. Yoo ,  Grace Lim ,  Sang Jin Lee

IPC: A61F2/28 ,  A61K38/18 ,  A61L27/36 ,  A61L27/38 ,  A61L27/48 ,  A61L27/56 ,  A61L27/58 ,  A61K35/32 ,  A61K35/34

CPC classification number: A61F2/28 ,  A61K35/32 ,  A61K35/34 ,  A61K38/18 ,  A61L27/3604 ,  A61L27/3817 ,  A61L27/3821 ,  A61L27/3826 ,  A61L27/383 ,  A61L27/3891 ,  A61L27/48 ,  A61L27/56 ,  A61L27/58 ,  A61L2430/02

Abstract: The invention pertains to methods of producing artificial composite tissue constructs that permit coordinated motion. Biocompatable structural matrices having sufficient rigidity to provide structural support for cartilage-forming cells and bone-forming cells are used. Biocompatable flexible matrices seeded with muscle cells are joined to the structural matrices to produce artificial composite tissue constructs that are capable of coordinated motion.

Abstract translation: 本发明涉及生产允许协调运动的人造复合组织结构的方法。 使用具有足够刚性以提供软骨形成细胞和骨形成细胞的结构支持的生物相容性结构基质。 用肌肉细胞接种的生物相容的柔性基质连接到结构基质上以产生能够协同运动的人造复合组织构建体。

公开(公告)号：US09005972B2

公开(公告)日：2015-04-14

申请号：US14185090

申请日：2014-02-20

Applicant: Wake Forest University Health Sciences

Inventor： Tao Xu ,  James J. Yoo ,  Anthony Atala ,  Dennis Dice

IPC: A61K9/00 ,  B41M5/52 ,  B41M99/00 ,  C12N11/04 ,  A61K35/36 ,  A61L27/38 ,  A61L27/50 ,  A61L27/58 ,  B41J3/407 ,  C12N11/08 ,  D01D5/00 ,  D01F6/62 ,  A61K35/32 ,  A61K35/34 ,  A61F2/06

CPC classification number: A61K9/5089 ,  A61F2/062 ,  A61K35/32 ,  A61K35/34 ,  A61K35/36 ,  A61L27/38 ,  A61L27/50 ,  A61L27/58 ,  B33Y80/00 ,  B41J3/407 ,  C12N5/0655 ,  C12N11/04 ,  C12N11/08 ,  C12N2533/54 ,  C12N2533/56 ,  D01D5/0007 ,  D01F6/625

Abstract: Provided herein is an apparatus for printing cells which includes an electrospinning device and an inkjet printing device operatively associated therewith. Methods of making a biodegradable scaffold having cells seeded therein are also provided. Methods of forming microparticles containing one or more cells encapsulated by a substrate are also provided, as are methods of forming an array of said microparticles.

Abstract translation: 本文提供了一种用于印刷电池的装置，其包括静电纺丝装置和与其可操作地相关联的喷墨打印装置。 还提供了制备具有种子细胞的可生物降解的支架的方法。 还提供了形成包含一个或多个由基底包封的细胞的微粒的方法，以及形成所述微粒阵列的方法。

公开(公告)号：US20150079682A1

公开(公告)日：2015-03-19

申请号：US14382816

申请日：2013-02-27

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Baisong Lu ,  Qingguo Zhao ,  James Yoo ,  Anthony Atala

IPC: C12N15/85

CPC classification number: C12N15/85 ,  C12N2800/40 ,  C12N2840/007

Abstract: The invention includes compositions and methods for the selective expression of a target gene in a subset of cells. In certain embodiments, the present invention includes a construct comprising a first nucleic acid sequence comprising an episomal maintenance element and a second nucleic acid sequence comprising a target gene wherein the expression of the episomal maintenance element is regulated by a constitutive promoter and the expression of the target gene is regulated by a non-constitutive promoter. The construct is able to maintain episomal state, no matter whether the target gene is expressed in the cell.

Abstract translation: 本发明包括用于在细胞子集中选择性表达靶基因的组合物和方法。 在某些实施方案中，本发明包括构建体，其包含第一核酸序列，其包含附加型维持元件和包含靶基因的第二核酸序列，其中所述附加型维持元件的表达由组成型启动子调节， 靶基因由非组成型启动子调控。 无论目标基因是否在细胞中表达，该构建体能够保持附加型状态。