公开(公告)号：US20080014222A1

公开(公告)日：2008-01-17

申请号：US11729339

申请日：2007-03-28

申请人： Andrew Simmons ,  Karin Jooss ,  James Allison

发明人： Andrew Simmons ,  Karin Jooss ,  James Allison

IPC分类号： A61K39/00 ,  A61P35/00

CPC分类号： A61K39/0011 ,  A61K39/0008 ,  A61K39/39 ,  A61K39/39558 ,  A61K2039/505 ,  A61K2039/5152 ,  A61K2039/55516 ,  A61K2039/55522 ,  C07K16/2818

摘要： Cellular compositions and methods for inducing an immune response to tumor cells are described. The cellular compositions include a tumor antigen and cells that have been modified to express a cytokine and one or more of a tumor antigen, anti-CTLA4 antibody and an additional cytokine. The cellular compositions find utility in methods for treating cancer.

摘要翻译： 描述了诱导对肿瘤细胞的免疫应答的细胞组合物和方法。 细胞组合物包括肿瘤抗原和已被修饰以表达细胞因子和肿瘤抗原，抗CTLA4抗体和另外的细胞因子中的一种或多种的细胞。 细胞组合物可用于治疗癌症的方法。

公开(公告)号：US07250299B1

公开(公告)日：2007-07-31

申请号：US10031639

申请日：2000-04-26

申请人： Luigi Naldini ,  Thomas Dull ,  Anatoly Bukovsky ,  Deborah Farson ,  Rochelle Witt

发明人： Luigi Naldini ,  Thomas Dull ,  Anatoly Bukovsky ,  Deborah Farson ,  Rochelle Witt

IPC分类号： C12N15/867 ,  C12N15/83 ,  C12N15/64 ,  A61K48/00

CPC分类号： C12N15/86 ,  C12N2740/16043 ,  C12N2740/16045 ,  C12N2810/6081 ,  C12N2830/003

摘要： Lentiviral vectors modified at the 5′ LTR or both the 5′ and 3′ LTR are useful in the production of recombinant lentivirus vectors (See the Figure). Such vectors can be produced in the absence of a functional tat gene. Multiple transformation of the host cell with the vector carrying the transgene enhances virus production. The vectors can contain inducible or conditional promoters.

摘要翻译： 在5'LTR或5'和3'LTR两者修饰的慢病毒载体可用于重组慢病毒载体的产生（见图）。 这种载体可以在不存在功能性tat基因的情况下产生。 携带转基因载体的宿主细胞多次转化增强病毒生产。 载体可以含有诱导型或条件启动子。

公开(公告)号：US20070141706A1

公开(公告)日：2007-06-21

申请号：US11545917

申请日：2006-10-11

申请人： Yuanhao Li ,  Deborah Farson ,  Luqun Tao ,  DeChao Yu

发明人： Yuanhao Li ,  Deborah Farson ,  Luqun Tao ,  DeChao Yu

IPC分类号： C12N15/861 ,  C12N5/10

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/48 ,  C12N2830/60 ,  C12N2830/90 ,  C12N2840/44

摘要： Adenovirus packaging cell lines for growth of an E1A/E1B deficient adenovirus that is substantially free of replication competent adenovirus (RCA) are provided. Methods for producing adenovirus substantially free of RCA are also provided, wherein the adenovirus is grown in a cell line containing coding sequences for adenovirus E1A and E1B, which are operably linked to promoters that lack polynucleotide sequences sharing substantial sequence identity with the native adenovirus E1A and E1B promoters.

摘要翻译： 提供了基本上不含复制性腺病毒（RCA）的E1A / E1B缺陷型腺病毒生长的腺病毒包装细胞系。 还提供了用于产生基本上不含RCA的腺病毒的方法，其中腺病毒在含有腺病毒E1A和E1B编码序列的细胞系中生长，所述编码序列与腺病毒E1A和E1B的编码序列可操作地连接，所述编码序列与缺乏与天然腺病毒E1A具有实质序列同一性的多核苷酸序列的启动子连接， E1B启动子。

公开(公告)号：US07083971B1

公开(公告)日：2006-08-01

申请号：US09762476

申请日：2000-06-07

申请人： Michael Mendez ,  Mitchell Finer

发明人： Michael Mendez ,  Mitchell Finer

IPC分类号： C12N1/21

CPC分类号： C12N15/86 ,  C12N15/81 ,  C12N2710/10343 ,  C12N2800/30 ,  C12N2800/70

摘要： A recombinational approach and system for the cloning, manipulation and delivery of large nucleic acids is disclosed. Vectors relying on homologous recombination to mediate the isolation, manipulation and delivery of large nucleic acid segments are disclosed.

摘要翻译： 公开了用于大型核酸的克隆，操作和递送的重组方法和系统。 公开了依赖于同源重组以介导大核酸片段的分离，操作和递送的载体。

公开(公告)号：US07026164B2

公开(公告)日：2006-04-11

申请号：US10613106

申请日：2003-07-03

申请人： Yuanhao Li ,  Deborah Farson ,  Luqun Tao ,  DeChao Yu

发明人： Yuanhao Li ,  Deborah Farson ,  Luqun Tao ,  DeChao Yu

IPC分类号： C12N15/867 ,  C12N5/10 ,  C12N15/63

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/48 ,  C12N2830/60 ,  C12N2830/90 ,  C12N2840/44

摘要： Adenovirus packaging cell lines for growth of an E1A/E1B deficient adenovirus that is substantially free of replication competent adenovirus (RCA) are provided. Methods for producing adenovirus substantially free of RCA are also provided, wherein the adenovirus is grown in a cell line containing coding sequences for adenovirus E1A and E1B, which are operably linked to promoters that lack polynucleotide sequences sharing substantial sequence identity with the native adenovirus E1A and E1B promoters.

公开(公告)号：US20050124068A1

公开(公告)日：2005-06-09

申请号：US10947812

申请日：2004-09-22

申请人： Trini Arroyo ,  Yuanhao Li

发明人： Trini Arroyo ,  Yuanhao Li

IPC分类号： A61K35/76 ,  A61K35/761 ,  A61K48/00 ,  C07H21/04 ,  C12N5/08 ,  C12N7/00 ,  C12N15/861

CPC分类号： C12N15/86 ,  A61K35/761 ,  A61K48/0058 ,  C07H21/04 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2840/203

摘要： Liver cancer, particularly hepatocellular carcinoma specific promoter sequences and adenovirus vehicles are provided. By providing for transcriptional initiating regulation dependent upon transcription factors that are only active in specific, limited cell types, virus replication will be restricted to the target cells. The modified adenovirus may be used as a vehicle for introducing new genetic capability, particularly associated with cytotoxicity, for treating neoplasia.

摘要翻译： 提供肝癌，特别是肝细胞癌特异性启动子序列和腺病毒载体。 通过提供依赖于仅在特定的有限细胞类型中有活性的转录因子的转录起始调节，病毒复制将限于靶细胞。 修饰的腺病毒可以用作引入新的遗传能力的载体，特别是与细胞毒性有关的遗传能力用于治疗瘤形成。

公开(公告)号：US06900049B2

公开(公告)日：2005-05-31

申请号：US09392822

申请日：1999-09-09

申请人： De Chao Yu ,  Daniel R. Henderson

发明人： De Chao Yu ,  Daniel R. Henderson

IPC分类号： C12N15/09 ,  A61K35/76 ,  A61K48/00 ,  A61P35/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N7/02 ,  C12N15/86 ,  C12N15/861 ,  A01N63/00 ,  A61K39/00 ,  C12N5/00 ,  C12N15/00 ,  C12P21/06

CPC分类号： A61K48/0058 ,  A61K35/13 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2830/002 ,  C12N2830/007 ,  C12N2830/008

摘要： The present invention provides adenoviral vectors comprising cell status-specific transcriptional regulatory elements which confer cell status-specific transcriptional regulation on an adenoviral gene. A “cell status” is generally a reversible physiological and/or environmental state. The invention further provides compositions and host cells comprising the vectors, as well as methods of using the vectors.

摘要翻译： 本发明提供了包含赋予腺病毒基因的细胞状态特异性转录调节的细胞状态特异性转录调节元件的腺病毒载体。 “细胞状态”通常是可逆的生理和/或环境状态。 本发明还提供了包含载体的组合物和宿主细胞，以及使用载体的方法。

公开(公告)号：US20030207445A1

公开(公告)日：2003-11-06

申请号：US10425323

申请日：2003-04-29

申请人： CELL GENESYS, INC.

发明人： Cherylene A. Schauber ,  Christopher D. Pacheco

IPC分类号： C12N015/00 ,  C12N015/63 ,  C12N015/74 ,  C07H021/04 ,  C12N015/09 ,  C12N015/70 ,  C12N015/86

CPC分类号： C12N15/86 ,  C12N2740/16043 ,  C12N2740/16045 ,  C12N2740/16052 ,  C12N2810/60 ,  C12N2810/6081

摘要： The present invention provides a retroviral gene delivery system that resists complement inactivation through the incorporation of a complement regulatory protein into retroviral particles. In particular, the present invention provides a lentiviral packaging system comprising at least two vectors: a first vector which comprises a nucleotide sequence comprising a gag, a pol, or gag and pol genes; and a second vector which comprises a nucleotide sequence comprising a gene that encodes a complement regulatory protein (CRP) and, optionally, a gene that encodes a heterologous or functionally modified envelope protein. The genes encoding a heterologous or functionally modified envelope protein and a CRP are provided either together in a second nucleotide sequence, or separately in second and third nucleotide sequences. Producer cells comprising the packaging constructs of the present invention and a transgene can be used to produce recombinant retroviral particles for transgene delivery.

摘要翻译： 本发明提供了通过将补体调节蛋白并入逆转录病毒颗粒中抵抗补体失活的逆转录病毒基因递送系统。 特别地，本发明提供了包含至少两个载体的慢病毒包装系统：第一载体，其包含包含gag，pol或gag和pol基因的核苷酸序列; 和包含编码补体调节蛋白（CRP）的基因和任选地编码异源或功能修饰的包膜蛋白的基因的核苷酸序列的第二载体。 编码异源或功能修饰的包膜蛋白和CRP的基因一起提供在第二核苷酸序列中，或分别在第二和第三核苷酸序列中提供。 包含本发明的包装构建体和转基因的生产细胞可用于产生用于转基因递送的重组逆转录病毒颗粒。

公开(公告)号：US6051427A

公开(公告)日：2000-04-18

申请号：US517488

申请日：1995-08-21

申请人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas L. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

发明人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas L. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

IPC分类号： C12N15/09 ,  A61K48/00 ,  C07K14/15 ,  C07K14/72 ,  C07K14/725 ,  C07K14/73 ,  C07K16/10 ,  C12N5/00 ,  C12N5/10 ,  C12N7/00 ,  C12N7/04 ,  C12N15/867 ,  C12N15/87 ,  C12R1/91 ,  C12N15/86

CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/721 ,  C07K16/10 ,  C12N15/87 ,  C12N7/00 ,  A61K48/00 ,  C07K2319/00 ,  C12N2740/13022 ,  C12N2740/13023 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/13062

摘要： The invention provides a novel retroviral packaging system, in which retroviral packaging plasmids and packagable vector transcripts are produced from high expression plasmids after stable or transient transfection in mammalian cells. High titers of recombinant retrovirus are produced in these transfected mammalian cells and can then transduce a mammalian target cell by cocultivation or supernatant infection. The methods of the invention include the use of the novel retroviral packaging plasmids and vectors to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation or supernatant infection at high efficiencies. The invention is useful for the rapid production of high titer viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.

公开(公告)号：US5834256A

公开(公告)日：1998-11-10

申请号：US76299

申请日：1993-06-11

申请人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas J. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

发明人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas J. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

IPC分类号： C12N15/09 ,  A61K31/70 ,  A61K35/76 ,  A61K48/00 ,  C07H21/04 ,  C07K14/15 ,  C07K14/72 ,  C07K14/725 ,  C07K14/73 ,  C07K16/10 ,  C12N5/10 ,  C12N7/00 ,  C12N7/04 ,  C12N15/867 ,  C12N15/87 ,  C12R1/92 ,  C12P19/34 ,  C12N15/63 ,  C12N15/86

CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/721 ,  C07K16/10 ,  C12N15/86 ,  C12N15/87 ,  A61K48/00 ,  C07K2319/00 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/13062

摘要： The invention provides a novel retroviral packaging system, in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells. High titers of recombinant retrovirus are produced in infected cells. The methods of the invention include the use of the novel retroviral constructs to transduce primary human cells, including T cells and bone marrow stem cells, with foreign genes by cocultivation at high efficiencies. The invention is useful for the rapid production of high viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.

摘要翻译： 本发明提供了一种新的逆转录病毒包装系统，其中通过在人细胞中转染从高表达质粒产生逆转录病毒包装构建体和可包装载体转录物。 在感染的细胞中产生高滴度的重组逆转录病毒。 本发明的方法包括使用新的逆转录病毒构建体通过以高效率的共培养将外源基因转导到包括T细胞和骨髓干细胞的原代人细胞。 本发明可用于快速生产高病毒上清液，并且可以通过常规方法转导难以转导的高效细胞。