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公开(公告)号：US4087327A
公开(公告)日：1978-05-02
申请号：US676100
申请日：1976-04-12
申请人： Joseph Feder , Katharine Ku , Mau-Jung Kuo
发明人： Joseph Feder , Katharine Ku , Mau-Jung Kuo
摘要： A cell culture reactor system is provided for the growth of cells in vitro which employs elongate hollow or solid fibers arranged in a shallow layer configuration as a matrix for cell attachment in which the flow path of the culture media is directed substantially uniformly through the fiber layer and substantially transverse to the plane of the elongate axes of the fibers.
摘要翻译： 提供细胞培养反应器系统用于体外细胞的生长，其采用以浅层构型布置的细长的中空或固体纤维作为细胞附着的基质，其中培养基的流动路径基本均匀地通过纤维层 并且基本上横向于纤维的细长轴的平面。
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公开(公告)号：US4071618A
公开(公告)日：1978-01-31
申请号：US702398
申请日：1976-07-06
申请人： Takeo Konobe , Tetsuo Onoda , Koichi Ono
发明人： Takeo Konobe , Tetsuo Onoda , Koichi Ono
CPC分类号： C12N7/00 , C12N2740/11051 , C12N2740/14051
摘要： Process for preparing a virus disease live vaccine by employing culture of a quail embryonated egg or tissue culture of a quail embryo fibroblast for cultivation of the virus. The vaccine obtained according to the present invention is effective for immunization of animals and humans against the virus disease. The vaccine is avirulent, scarcely subject to induction of unfavorable factors accompanying the present tissue used for isolation of the virus therefrom, and economically productible.
摘要翻译： 通过使用鹌鹑胚胎培养的鹌鹑胚胎成纤维细胞的培养物或用于培养病毒的鹌鹑胚成纤维细胞的组织培养来制备病毒病活疫苗的方法。 根据本发明获得的疫苗对于针对病毒病的动物和人的免疫是有效的。 疫苗无毒，很少受到伴随用于分离病毒的本组织伴随的不利因素的诱导，并且经济地生产。
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73.发明授权Diploid porcine embryonic cell strains, cultures produced therefrom, and use of said cultures for production of vaccines 失效二倍体猪胚胎细胞株，由其产生的培养物，以及使用所述培养物生产疫苗
公开(公告)号：US4070453A
公开(公告)日：1978-01-24
申请号：US700251
申请日：1976-06-25
IPC分类号： A61K39/225 , C12N7/00 , A61K39/12 , C12B3/00 , C12K9/00
CPC分类号： C12N7/00 , A61K39/12 , A61K39/225 , A61K47/42 , A61K9/0019 , A61K2039/5254 , A61K2039/552 , C12N2770/20034 , Y10S424/815
摘要： Diploid porcine embryonic cell strains, method of growing same from porcine embryonic tissue, culturing and subculturing said diploid cell strains, and preparing a virus vaccine (exemplary of the virus vaccine thus prepared is a porcine transmissible gastroenteritis virus vaccine) from said diploid cell strain cultures. A novel nutrient growth medium composition for culturing and subculturing by serial subcultivation is also disclosed.
摘要翻译： 二倍体猪胚胎细胞株，从猪胚胎组织生长相同的方法，培养和传代培养所述二倍体细胞株，并从所述二倍体细胞株培养物制备病毒疫苗（如此制备的病毒疫苗的示例是猪传染性胃肠炎病毒疫苗） 。 还公开了通过连续亚培养培养和传代培养的新型营养生长培养基组合物。
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公开(公告)号：US4060457A
公开(公告)日：1977-11-29
申请号：US647177
申请日：1976-01-07
申请人： Masahiko Iizuka , Jiro Suzuki , Sigeyasu Kobayashi
发明人： Masahiko Iizuka , Jiro Suzuki , Sigeyasu Kobayashi
摘要： An apparatus for growing animal cells of this invention comprises tubes with a hole near bottom of each tube and a variable angle tube holder. Cells may be attached and proliferated in the tubes which contain a nutrient medium, which can be removed simply from the tubes through holes by changing the angle of the tubes. Cell sheets in many tubes can be washed simply, rapidly and simultaneously.
摘要翻译： 用于生长本发明的动物细胞的装置包括在每个管的底部附近具有孔的管和可变角管保持器。 细胞可以在含有营养培养基的管中附着和增殖，其可以通过改变管的角度从管中通过孔去除。 许多管中的细胞片可以简单，快速和同时地洗涤。
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公开(公告)号：US4059486A
公开(公告)日：1977-11-22
申请号：US738513
申请日：1976-11-03
申请人： William R. Tolbert
发明人： William R. Tolbert
IPC分类号： A61K35/12 , A61P43/00 , C07K14/005 , C07K14/195 , C07K14/435 , C07K14/515 , C07K14/52 , C12N5/18 , C12P1/00 , C12P21/00 , C12K9/00
CPC分类号： C07K14/52 , C07K14/515
摘要： SV3T3 cells are rapidly propagated in vitro to elaborate MIF and TAF by first growing on a surface and thereafter transferring an inoculum of the growing cells into an agitated liquid suspension of nutrient culture medium, incubating at 30.degree.-38.degree. C while continuing the agitation of the medium, periodically transferring the growing cells into fresh medium and continuing the incubation with agitation of the medium, whereby said cells are proliferated in aggregates and elaborate MIF and TAF in significantly detectable amounts.
摘要翻译： SV3T3细胞在体外迅速繁殖，通过首先在表面上生长，然后将生长细胞的接种物转移到营养培养基的搅拌液体悬浮液中，在30℃-38℃下孵育，同时继续搅拌，从而制备MIF和TAF 培养基中，将生长的细胞周期性地转移到新鲜的培养基中，并继续培养培养基，由此所述细胞以聚集体增殖，并以显着可检测的量精制MIF和TAF。
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公开(公告)号：US4046875A
公开(公告)日：1977-09-06
申请号：US751902
申请日：1976-12-17
IPC分类号： A61K39/225 , A61K39/12 , C12K9/00
CPC分类号： A61K39/225 , A61K39/12 , A61K2039/5254 , A61K2039/552 , C12N2770/20034 , Y10S424/815
摘要： A TGE virus is attenuated in organ culture of the colon of swine. After a number of passages, the resulting attenuated virus, if administered to swine will not seriously damage the villi of the small intestine, so that absorption of protein and other vital materials is not seriously hampered. The swine may suffer slight side effects, but are then immune to virulent TGE virus which is normally fatal to piglets.
摘要翻译： TGE病毒在猪结肠器官培养中减弱。 经过多次传代之后，如果施用于猪，所产生的减毒病毒不会严重损伤小肠绒毛，使蛋白质等重要物质的吸收没有受到严重的阻碍。 猪可能会受到轻微的副作用，然后免疫对通常对仔猪致命的毒性TGE病毒。
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公开(公告)号：US4033825A
公开(公告)日：1977-07-05
申请号：US547703
申请日：1975-02-06
CPC分类号： C12M41/14 , C12M23/24 , C12M23/40 , C12M23/48 , Y10S435/809
摘要： A cell culture system includes an incubator chamber, and a carriage that is removable from the incubator chamber and that carries a plurality of cell culture chambers. Also mounted on the carriage are a manifold structure having supply and return conduits, detachable coupling structure for connecting the cell culture chambers to the manifold structure and pump structure for circulating nutrient material between the manifold structure and the connected cell culture chambers.
摘要翻译： 细胞培养系统包括培养箱和可从培养箱移除并携带多个细胞培养室的托架。 还安装在托架上的是具有供应和返回管道的歧管结构，用于将细胞培养室连接到歧管结构的可拆卸联接结构和用于在歧管结构和连接的细胞培养室之间循环营养物质的泵结构。
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公开(公告)号：US4016036A
公开(公告)日：1977-04-05
申请号：US632037
申请日：1975-11-14
申请人： Howard Green , James G. Rheinwald
发明人： Howard Green , James G. Rheinwald
CPC分类号： C12N5/0629 , C12N2502/13
摘要： A method is disclosed for serially culturing keratinocytes such as human epidermal cells and teratomal keratinocytes. These can be serially grown in culture to produce colonies and eventually stratified squamous epithelium by including in the culture fibroblast cells or medium harvested from fibroblast cultures. When fibroblast cells are used, they are treated to prevent their multiplication, and they are also introduced into the cultures at a controlled density.
摘要翻译： 公开了用于连续培养角质细胞如人表皮细胞和畸齿角质形成细胞的方法。 这些可以在培养物中连续生长以产生菌落并最终分层鳞状上皮，包括培养成纤维细胞或从成纤维细胞培养物收获的培养基。 当使用成纤维细胞时，它们被处理以防止它们的增殖，并且它们也以受控的密度引入培养物中。
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公开(公告)号：US4004981A
公开(公告)日：1977-01-25
申请号：US573655
申请日：1975-05-01
摘要： Apparatus for physically removing cells in sterile manner from a disc stack on which the cells have been cultured comprises a substantially shaft-like member having at least one rod-like member mounted at substantially a right angle to the axis of the shaft.
摘要翻译： 用于以消毒方式物理去除细胞已经培养的盘堆叠的细胞的装置包括具有至少一个棒状部件的大致轴状部件，所述杆状部件以与轴的轴线大致成直角的角度安装。
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80.发明授权
公开(公告)号：US3989591A
公开(公告)日：1976-11-02
申请号：US502843
申请日：1974-09-03
申请人： Lance A. Liotta
发明人： Lance A. Liotta
CPC分类号： G01N35/00029 , C12Q1/24 , G01N2001/2833 , G01N2035/00099 , G01N2035/1025 , Y10S435/808
摘要： The specification and drawings disclose methods and apparatus for separating and/or isolating bacteria and tissue cells for testing or rapid automatic scanning. The invention uses an expansible membrane on which the tissue cell or bacteria sample is placed and thereafter expanded to cause the sample to be separated. Apparatus is disclosed for facilitating stretching of the membrane and automatically scanning it.
摘要翻译： 说明书和附图公开了用于分离和/或分离用于测试或快速自动扫描的细菌和组织细胞的方法和装置。 本发明使用其上放置有组织细胞或细菌样品的可膨胀膜，然后膨胀以使样品分离。 公开了用于促进膜的拉伸并自动扫描的装置。
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