公开(公告)号：US08148130B2

公开(公告)日：2012-04-03

申请号：US12039803

申请日：2008-02-29

申请人： Carl R. Alving ,  Venigalla Rao

发明人： Carl R. Alving ,  Venigalla Rao

IPC分类号： C12N7/01 ,  A61K15/21 ,  A61K9/127 ,  C12N15/73

CPC分类号： A61K39/39 ,  A61K39/12 ,  A61K39/21 ,  A61K39/385 ,  A61K2039/55555 ,  A61K2039/55572 ,  A61K2039/6075 ,  C07K14/005 ,  C07K2319/735 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2795/10122

摘要： T4 bacteriophages are bound to substrates such as liposomes using a binder.

摘要翻译： 使用粘合剂将T4噬菌体与底物例如脂质体结合。

公开(公告)号：USRE42130E1

公开(公告)日：2011-02-08

申请号：US11986963

申请日：2007-11-26

申请人： Katherine S. Bowdish ,  Shana Frederickson ,  Martha Wild

发明人： Katherine S. Bowdish ,  Shana Frederickson ,  Martha Wild

IPC分类号： C12N15/63 ,  C12N15/70 ,  C12N15/73 ,  C12N15/64 ,  C12N15/65 ,  C07H21/04

CPC分类号： C40B40/02 ,  C12N15/1037 ,  C12N15/70

摘要： Phagemid vectors containing a sequence of features between a Col E1 origin and an f1 origin are useful for display of polypeptides or proteins, including antibody libraries.

摘要翻译： 含有Col E1起点和f1起点之间特征序列的噬菌粒载体可用于显示多肽或蛋白质，包括抗体文库。

公开(公告)号：US06964845B1

公开(公告)日：2005-11-15

申请号：US09147693

申请日：1997-08-21

申请人： Werner Lubitz ,  Wolfgang Jechlinger ,  Michael Szostak ,  Angela Witte

发明人： Werner Lubitz ,  Wolfgang Jechlinger ,  Michael Szostak ,  Angela Witte

IPC分类号： C12N15/09 ,  A61K39/02 ,  A61K48/00 ,  A61P31/04 ,  C12N1/21 ,  C12N15/73 ,  C12N15/00 ,  C07H21/04 ,  C12N1/20 ,  C12Q1/68

CPC分类号： C12N15/73

摘要： The present invention concerns a method for selecting New PR or PL operator sequences from lambdoid phages which have different thermostability compared to the wild-type sequence with regard to binding a repressor. In addition new mutated PR or PL operator sequences and their use for the temperature-regulated expression of genes and for production of improved vaccines is disclosed.

摘要翻译： 本发明涉及从与野生型序列相比具有不同热稳定性的关于结合阻遏物的羊羔噬菌体中选择新的P或P L L操纵子序列的方法 。 此外，公开了新的突变的PR或P L L operator operator operator operator operator operator operator and of of of。。。。。。。。。。。。。。。。。。。。。。。

公开(公告)号：US20050090010A1

公开(公告)日：2005-04-28

申请号：US10469508

申请日：2002-02-25

申请人： Yoshihide Hayashizaki ,  Piero Carninci

发明人： Yoshihide Hayashizaki ,  Piero Carninci

IPC分类号： C12N15/09 ,  C12N7/00 ,  C12N15/65 ,  C12N15/73 ,  C12P19/34 ,  C12N15/74

CPC分类号： C12N15/73 ,  C12N15/65

摘要： The invention discloses a family of cloning vectors capable of cloning nucleic acid inserts of interest of long sizes, with low or reduced background and high efficiency of excision and method for preparing these vectors and library thereof. As example, it is disclosed a cloning vector comprising a construction vector segment (CS) and a replaceable segment (RS), wherein the size of CS is: 36.5 kb≦CS

摘要翻译： 本发明公开了一种克隆载体，其能够克隆具有低或低背景和高效切割的长尺寸感兴趣的核酸插入物及其制备方法及其文库。 例如，公开了包含构建载体片段（CS）和可替换片段（RS）的克隆载体，其中CS的大小为：36.5kb <= CS <38​​kb，优选CS为37.5kb，包含lox重组 用于网关样重组的Cre重组和/或att重组位点的位点，优选还包括选自以下的背景降低系统：ccdB基因，lox序列，lacZ基因和由限制性内切核酸酶识别的不对称位点序列 。

公开(公告)号：US6156300A

公开(公告)日：2000-12-05

申请号：US426571

申请日：1995-04-21

申请人： Martha B. Ladner ,  Janelle A. Noble ,  George A. Martin ,  Ernest S. Kawasaki ,  Mazie Yee Coyne ,  Robert F. Halenbeck ,  Kirston E. Koths

发明人： Martha B. Ladner ,  Janelle A. Noble ,  George A. Martin ,  Ernest S. Kawasaki ,  Mazie Yee Coyne ,  Robert F. Halenbeck ,  Kirston E. Koths

IPC分类号： A61K38/00 ,  C07K14/53 ,  C12N15/73 ,  A61K38/19

CPC分类号： C12N15/73 ,  C07K14/53 ,  A61K38/00

摘要： A colony stimulating factor, CSF-1, is a lymphokine useful in regulating the immune system. is a lymphokine useful in overcoming the immunosuppression induced by chemotherapy or resulting from other causes. CSF-1 is obtained in usable amounts by recombinant methods, including cloning and expression of the murine and human DNA sequences encoding this protein. Both "long" and "short" forms of this protein and muteins corresponding to the cDNA-encoded forms are disclosed.

摘要翻译： 集落刺激因子CSF-1是可用于调节免疫系统的淋巴因子。 是用于克服化疗诱导的免疫抑制或由其它原因引起的淋巴因子。 通过重组方法获得可用量的CSF-1，包括克隆和表达编码该蛋白质的鼠和人DNA序列。 公开了这种蛋白质的“长”和“短”形式和对应于cDNA编码形式的突变蛋白。

公开(公告)号：US6146851A

公开(公告)日：2000-11-14

申请号：US426243

申请日：1995-04-21

申请人： Martha B. Ladner ,  Janelle N. Van Arsdell ,  George A. Martin ,  Ernest S. Kawasaki ,  Mazie Yee Coyne ,  Robert F. Halenbeck ,  Kirston E. Koths

发明人： Martha B. Ladner ,  Janelle N. Van Arsdell ,  George A. Martin ,  Ernest S. Kawasaki ,  Mazie Yee Coyne ,  Robert F. Halenbeck ,  Kirston E. Koths

IPC分类号： A61K38/00 ,  C07K14/53 ,  C12N1/21 ,  C12N15/27 ,  C12N15/73 ,  C12N5/00 ,  C12N15/63

CPC分类号： C07K14/53 ,  C12N15/73 ,  A61K38/00 ,  Y10S930/14

摘要： A colony stimulating factor, CSF-1, is a lymphokine useful in regulating the immune system is a lymphokine useful in overcoming the immunosuppression induced by chemotherapy or resulting from other causes. CSF-1 is obtained in usable amounts by recombinant methods, including cloning and expression of the murine and human DNA sequences encoding this protein. Both "long" and "short" forms of this protein and muteins corresponding to the cDNA-encoded forms are disclosed.

摘要翻译： 集落刺激因子CSF-1是可用于调节免疫系统的淋巴因子，是可用于克服化疗诱导的免疫抑制或由其他原因引起的淋巴因子。 通过重组方法获得可用量的CSF-1，包括克隆和表达编码该蛋白质的鼠和人DNA序列。 公开了这种蛋白质的“长”和“短”形式和对应于cDNA编码形式的突变蛋白。

公开(公告)号：US6068993A

公开(公告)日：2000-05-30

申请号：US886967

申请日：1997-07-02

申请人： Spartaco Astolfi Filho ,  Beatriz Dolabela de Lima ,  Josef Ernst Thiemann ,  Heloisa Ribeiro Tunes de Sousa ,  Luciano Vilela

发明人： Spartaco Astolfi Filho ,  Beatriz Dolabela de Lima ,  Josef Ernst Thiemann ,  Heloisa Ribeiro Tunes de Sousa ,  Luciano Vilela

IPC分类号： C07K14/62 ,  C12N15/00 ,  C12N15/73 ,  C12P21/02 ,  C42N15/09

CPC分类号： C12N15/73 ,  C07K14/62 ,  C12N15/10 ,  C12N15/64 ,  C12P21/02

摘要： The present invention relates to a vector for expression of a heterologous protein by a Gram negative bacteria, wherein the vector includes a nucleic acid such as DNA encoding the following: an origin of replication region; optionally and preferably a selection marker; a promoter; an initiation region such as translation initiation region and/or a ribosome binding site, at least one restriction site for insertion of heterologous nucleic acid, e.g. DNA, encoding the heterologous protein, and a transcription terminator. The inventive vector may contain DNA encoding the heterologous protein, e.g., pro-insulin such as pro-insulin with a His tag. Additionally, the invention provides a method for extracting a recombinant protein from within a recombinant Gram negative bacteria having a cell membrane, without lysing the bacteria, as well as a method for purifying an isolated recombinant human insulin, wherein the isolated recombinant human pro-insulin is subjected to sulfitolysis, Ni-chelation chromatography, renaturation, limited proteolysis and chromatography separation to provide purified, isolated, recombinant human insulin.

公开(公告)号：US6054291A

公开(公告)日：2000-04-25

申请号：US871302

申请日：1997-06-09

申请人： Marian Gorecki ,  Avigdor Levanon ,  Amos Oppenheim ,  Tikva Vogel

发明人： Marian Gorecki ,  Avigdor Levanon ,  Amos Oppenheim ,  Tikva Vogel

IPC分类号： A61K38/00 ,  C07K14/61 ,  C12N9/02 ,  C12N15/73 ,  C12P21/02

CPC分类号： C12N9/0089 ,  C07K14/61 ,  C12N15/73 ,  A61K38/00

摘要： An improved vector upon introduction into a suitable bacterial host containing the thermolabile repressor C.sub.I renders the host cell capable, upon increasing the temperature of the host cell to a temperature at which the repressor is destroyed, of effecting expression of a desired gene inserted into the vector and production of polypeptide encoded by the gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: a DNA sequence which contains the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site for binding antiterminator N protein produced by the host cell; a DNA sequence which contains a ribosomal binding site for rendering the mRNA of the desired gene capable of binding to ribosomes within the host cell; an ATG initiation codon or a DNA sequence which is converted into an ATG initiation codon upon insertion of the desired gene into the vector; a restriction enzyme site for inserting the desired gene into the vector in phase with the ATG initiation codon; and additionally a DNA sequence which contains an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell and a DNA sequence which contains a gene associated with a selectable or identifiable trait which is manifested when the vector is present in the host cell.

摘要翻译： 在引入含有不耐热阻遏物CI的合适的细菌宿主中时，改进的载体使宿主细胞在将宿主细胞的温度升高至阻遏物被破坏的温度时能够实现插入到载体中的所需基因的表达 并产生由该基因编码的多肽。 载体是双链DNA分子，其以5'至3'的顺序包括以下：含有来自λ噬菌体的启动子和操纵子PLOL的DNA序列; 用于结合由宿主细胞产生的抗终止子N蛋白的N利用位点; 一个DNA序列，其含有一个核糖体结合位点，用于使所需基因的mRNA能够结合宿主细胞内的核糖体; ATG起始密码子或将所需基因插入载体后转化为ATG起始密码子的DNA序列; 用于将所需基因插入与ATG起始密码子同相载体的限制酶位点; 另外还包含含有能够在宿主细胞中自主复制的细菌质粒的复制起点的DNA序列和含有与可选择或可识别的性状相关的基因的DNA序列，所述基因在载体存在于宿主细胞中时显示 。

公开(公告)号：US5871907A

公开(公告)日：1999-02-16

申请号：US150002

申请日：1994-03-31

申请人： Gregory Paul Winter ,  Kevin Stuart Johnson ,  Andrew David Griffiths ,  Andrew John Hammond Smith

发明人： Gregory Paul Winter ,  Kevin Stuart Johnson ,  Andrew David Griffiths ,  Andrew John Hammond Smith

IPC分类号： C07K16/00 ,  C07K16/18 ,  C07K16/22 ,  C07K16/24 ,  C07K16/28 ,  C07K16/30 ,  C07K16/34 ,  C07K16/40 ,  C07K16/42 ,  C07K16/44 ,  C07K16/46 ,  C07K19/00 ,  C12N15/00 ,  C12N15/10 ,  C12N15/13 ,  C12N15/62 ,  C12N15/73 ,  C12P21/08 ,  C40B40/02 ,  G01N33/53 ,  G01N33/531 ,  G01N33/68 ,  C12Q1/68 ,  C12N15/70 ,  C12P21/02

CPC分类号： C40B40/02 ,  C07K16/00 ,  C07K16/18 ,  C07K16/22 ,  C07K16/241 ,  C07K16/28 ,  C07K16/2812 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/3007 ,  C07K16/34 ,  C07K16/40 ,  C07K16/4208 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  G01N33/53 ,  G01N33/6857 ,  B01J2219/00718 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735

摘要： The invention provides methods and kits for producing specific binding pairs (sbp) members. Populations of polypeptide chain components of sbp members are combined to form libraries of sbps displayed by secreted replicable genetic display packages (rgdp). At least one of the polypeptide chains is expressed as a fusion with a component of an rgdp which thereby displays that polypeptide chain at the surface of rgdp. At least one population of polypeptide chains is expressed from nucleic acid which is capable of being packaged using a component of an rgdp, whereby the genetic material of rgdps produced encodes a polypeptide chain. The methods enable production of libraries of multimeric sbp members from a very large number of possible combinations. In one embodiment of the invention a method employs "chain shuffling" in the production of sbp members of desired specificity for a counterpart sbp member. Selection procedures are also described.

摘要翻译： PCT No.PCT / GB92 / 00883 Sec。 371日期1994年3月31日 102（e）1994年3月31日PCT提交1992年5月15日PCT公布。 WO92 / 20791 PCT出版物 日期1992年11月26日本发明提供了用于产生特异性结合对（sbp）成员的方法和试剂盒。 将sbp成员的多肽链组分的群体组合以形成由分泌的可复制的遗传显示包（rgdp）显示的sbps的文库。 至少一个多肽链表达为与rgdp的组分的融合物，从而在rgdp的表面显示该多肽链。 至少一个多肽链群由能够使用rgdp的组分包装的核酸表达，由此产生的rgdps的遗传物质编码多肽链。 该方法能够从非常大量的可能组合生产多聚体sbp成员的文库。 在本发明的一个实施方案中，一种方法在生产对于对应物质成员的所需特异性的sbp成员中使用“链洗牌”。 还描述了选择程序。

公开(公告)号：US5759852A

公开(公告)日：1998-06-02

申请号：US266908

申请日：1994-06-27

申请人： Richard J. Kirschner ,  John Edward Mott ,  Frances M. Eckenrode ,  David Paul Brunner

发明人： Richard J. Kirschner ,  John Edward Mott ,  Frances M. Eckenrode ,  David Paul Brunner

IPC分类号： C12N1/21 ,  A61K38/00 ,  C07K14/73 ,  C12N15/09 ,  C12N15/67 ,  C12P21/02 ,  C12R1/19 ,  C07H21/04 ,  C12N15/73

CPC分类号： C07K14/70514 ,  C12N15/67 ,  A61K38/00 ,  C07K2319/00

摘要： Disclosed are expression vectors useful as vectors in recombinant methods to facilitate expression of exogenous genes in E. coli. Specifically, the disclosed expression vector has the following elements in operable linkage: the PL6m promoter, the TAT32 ribosome binding site and a gene encoding a heterologous polypeptide, Also disclosed are E. coli host cells transformed with this expression vector.

摘要翻译： 公开了用作重组方法中的载体的表达载体，以促进外源基因在大肠杆菌中的表达。 具体地，所公开的表达载体在可操作的连接中具有以下元件：PL6m启动子，TAT32核糖体结合位点和编码异源多肽的基因。还公开了用该表达载体转化的大肠杆菌宿主细胞。