公开(公告)号：US20190071640A1

公开(公告)日：2019-03-07

申请号：US16057274

申请日：2018-08-07

Applicant: LIFE TECHNOLOGIES AS

Inventor： Tanja AARVAK ,  Anne-Marie RASMUSSEN ,  Gunnar KVALHEIM ,  Walter Gabriell Borelli PIEDRAS ,  Anne BRUNSVIG

IPC: C12N5/0783 ,  A61K35/17 ,  A61K31/12

Abstract: Methods are disclosed for the generation of immunosuppressive regulatory T cells. The methods can include contacting a population of CD4+CD25− T cells with a T cell receptor (TCR)/CD3 activator, a TCR co-stimulator activator, and rapamycin. Kits for the generation of immunosuppressive regulatory T cells, methods of use, and cell populations are also disclosed.

公开(公告)号：US09828453B2

公开(公告)日：2017-11-28

申请号：US15212532

申请日：2016-07-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Hofslokken Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08F230/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：USRE46207E1

公开(公告)日：2016-11-22

申请号：US29454427

申请日：2013-05-09

Applicant: LIFE TECHNOLOGIES AS

Inventor： Gaute Brede ,  Darren Ellis ,  Tine Hildisch ,  Jeremy (“Jez”) Mundy ,  Lars Nokleby ,  Erlend Ragnhildstveit ,  Richard Toften ,  Kornelija Zgonc

公开(公告)号：US20160326291A1

公开(公告)日：2016-11-10

申请号：US15212532

申请日：2016-07-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Hofslokken KJUS ,  Geir FONNUM ,  Bruce BRANCHAUD ,  Lai-Qiang YING ,  Steven M. MENCHEN ,  Dmitriy GREMYACHINSKIY ,  Hee Chol KANG

IPC: C08F230/08

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US09464316B2

公开(公告)日：2016-10-11

申请号：US14035890

申请日：2013-09-24

Applicant: LIFE TECHNOLOGIES AS

Inventor： Erling Sigurd Finne

IPC: C07H21/00 ,  C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1013

Abstract: A method of isolating nucleic acid from a sample is provided that includes contacting a sample that includes nucleic acid with a solid support, wherein the surface of the solid support includes groups that can complex with the nucleic acid, in the presence of a solution that includes an oligoethylene glycol having 10 or fewer ethylene oxide-units and a salt comprising a monovalent or divalent metal ion, whereby soluble nucleic acid in said sample binds to the surface of the support; separating the solid support with bound nucleic acid from the sample; and eluting the bound nucleic acid from the solid support, thereby isolating nucleic acid from the sample. Also provided is a method of washing nucleic acids bound to a solid support that includes providing a solid support, wherein nucleic acid molecules are bound to the surface of the solid support; and washing the solid support with a solution that includes an oligoethylene glycol having 10 or fewer ethylene oxide units.

Abstract translation: 提供了从样品中分离核酸的方法，其包括将包含核酸的样品与固体支持物接触，其中所述固体支持物的表面包括可与核酸复合的基团，所述溶液包括 具有10个或更少环氧乙烷单元的低聚乙二醇和包含一价或二价金属离子的盐，由此所述样品中的可溶性核酸与载体表面结合; 从样品中分离具有结合核酸的固体支持物; 并从固体支持物洗脱结合的核酸，从而从样品中分离核酸。 还提供了洗涤与固体支持物结合的核酸的方法，其包括提供固体支持物，其中核酸分子与固体支持物的表面结合; 并用包含具有10个或更少环氧乙烷单元的低聚乙二醇的溶液洗涤固体载体。

公开(公告)号：US09309368B2

公开(公告)日：2016-04-12

申请号：US14152793

申请日：2014-01-10

Applicant: LIFE TECHNOLOGIES AS

Inventor： Arne Jørgedal ,  Elin Marie Aksnes ,  Geir Fonnum ,  Astrid Molteberg ,  Rolf Nordal ,  Henning Pettersen ,  Tollef Tårneby ,  Solveig Staale ,  Ellen Weng ,  Finn Hansen ,  Silje Nordbø ,  Oddvar Aune ,  Arvid Berge ,  John Bjørgum ,  Turid Ellingsen ,  John Ugelstad

IPC: C08J9/16 ,  C08F257/02 ,  C08F265/04 ,  C08F265/10 ,  C08F291/00 ,  C08J9/224

CPC classification number: C08J9/16 ,  C08F2/22 ,  C08F257/02 ,  C08F265/04 ,  C08F265/10 ,  C08F291/00 ,  C08J9/22 ,  C08J9/224 ,  C08J2203/14 ,  C08J2325/06 ,  C08J2347/00

Abstract: A process for the preparation of monodisperse polymer particles which are formed by contacting monomers with aqueous dispersions comprising monodisperse swellable seed polymers/oligomers, and initiating polymerization in the presence of a steric stabilizer. The resulting swollen seed particles are characterized by the particle mode diameter.

Abstract translation: 通过使单体与包含单分散可溶胀种子聚合物/低聚物的水性分散体接触而形成的单分散聚合物颗粒的制备方法，以及在空间稳定剂存在下引发聚合。 所得的溶胀种子颗粒的特征在于颗粒模式直径。

公开(公告)号：US09139666B2

公开(公告)日：2015-09-22

申请号：US14082368

申请日：2013-11-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete I. Modahl ,  Nini H. Kjus ,  Astrid E. Molteberg ,  Diem Tran ,  Jo Aaserud ,  Talha M. Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C12Q1/68 ,  C08F8/34 ,  C07F7/18 ,  C08F6/24 ,  C08F130/08 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F230/08 ,  C08F222/38 ,  C08F8/32

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

Abstract translation: 形成颗粒的方法包括在水性悬浮液中的分散相中聚合具有疏水保护基团的亲水性单体的多个聚合单元，从而形成包含多个疏水保护基团的聚合物颗粒。 该方法还包括将聚合物颗粒转化为亲水性颗粒。

公开(公告)号：US20140291576A1

公开(公告)日：2014-10-02

申请号：US14183999

申请日：2014-02-19

Applicant: Life Technologies AS

Inventor： Arne DEGGERDAL ,  Frank Larsen

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample.

Abstract translation: 本发明提供了从样品中分离核酸的方法，所述方法包括使所述样品与洗涤剂和固体支持物接触，由此使所述样品中的可溶性核酸与载体结合，并将所述载体与结合的核酸从 例子。 当本发明的方法用于分离DNA时，可以方便地与进一步的步骤相结合以从同一样品中分离RNA。

公开(公告)号：US20140256836A1

公开(公告)日：2014-09-11

申请号：US14152793

申请日：2014-01-10

Applicant: LIFE TECHNOLOGIES AS

Inventor： Arne JØRGEDAL ,  Elin Marie AKSNES ,  Geir FONNUM ,  Astrid MOLTEBERG ,  Rolf NORDAL ,  Henning PETTERSEN ,  Tollef TÂRNEBY ,  Solveig STAALE ,  Ellen WENG ,  Finn HANSEN ,  Silje NORDBØ ,  Oddvar AUNE ,  Arvid BERGE ,  John BJØRGUM ,  Turid ELLINGSEN ,  John UGELSTAD

IPC: C08J9/16 ,  C08J9/224

CPC classification number: C08J9/16 ,  C08F2/22 ,  C08F257/02 ,  C08F265/04 ,  C08F265/10 ,  C08F291/00 ,  C08J9/22 ,  C08J9/224 ,  C08J2203/14 ,  C08J2325/06 ,  C08J2347/00

Abstract: A process for the preparation of monodisperse polymer particles which are formed by contacting monomers with aqueous dispersions comprising monodisperse swellable seed polymers/oligomers, and initiating polymerization in the presence of a steric stabilizer. The resulting swollen seed particles are characterized by the particle mode diameter.

Abstract translation: 通过使单体与包含单分散可溶胀种子聚合物/低聚物的水性分散体接触而形成的单分散聚合物颗粒的制备方法，以及在空间稳定剂存在下引发聚合。 所得的溶胀种子颗粒的特征在于颗粒模式直径。

公开(公告)号：US20140073756A1

公开(公告)日：2014-03-13

申请号：US14081224

申请日：2013-11-15

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Nini H. KJUS ,  Geir FONNUM ,  Bruce BRANCHAUD ,  Lai-Qiang Ying ,  Steven M. MENCHEN ,  Dmitriy GREMYACHINSKIY ,  Hee Chol KANG

IPC: C07F7/18 ,  C08F236/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

Abstract translation: 描述了甲硅烷基保护的二丙烯酰胺化合物。 形成这种化合物的方法包括在第一反应条件下将甲硅烷基化试剂与羟基化二胺化合物混合以在第一溶液中形成产物，将产物与第一溶液分离，并在第二反应条件下将产物与丙烯酰氯混合 形成甲硅烷基保护的二丙烯酰胺化合物的第二溶液。