公开(公告)号：US11739290B2

公开(公告)日：2023-08-29

申请号：US17555336

申请日：2021-12-17

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/68 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C12N15/113 ,  B01D63/08 ,  B01D69/06 ,  B01D71/02 ,  C12N15/10

CPC classification number: C12M47/04 ,  C12M23/44 ,  C12M29/04 ,  C12M33/00 ,  C12M47/02 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12N15/68 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/87 ,  C12N15/907 ,  B01D63/08 ,  B01D69/06 ,  B01D71/02 ,  B01D2201/0407 ,  C12N15/102 ,  C12N15/90 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2830/001 ,  C12N2830/002

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20220267812A1

公开(公告)日：2022-08-25

申请号：US17676720

申请日：2022-02-21

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Daniel Held ,  Eric Abbate ,  Katherine Noon

IPC: C12P7/10 ,  C12N15/10 ,  C12N1/20 ,  C12N15/70 ,  C12N9/22 ,  C12N15/11 ,  C12N15/52 ,  C10L1/02

Abstract: The present disclosure provides multiplexed engineered cells, automated multi-module instruments and methods of producing biofuel producing cells for enhanced production of biofuels. This platform empowers users to design genetic variant libraries of insertions, swaps, and/or deletions, that can be intentionally or randomly positioned across the entire genome to generate engineered cell populations with improved properties for several common biofuel applications including, but not limited to, improved tolerance to biomass inhibitors, increased thermo-tolerance, increased ethanol production and/or tolerance, expanded carbon utilization abilities, and increased utilization of heterologous proteins or pathways.

公开(公告)号：US20220259543A1

公开(公告)日：2022-08-18

申请号：US17734037

申请日：2022-04-30

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky ,  Michael Graige

IPC: C12M1/42 ,  C12M3/00 ,  C12N15/11 ,  C12N15/90 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US11365383B1

公开(公告)日：2022-06-21

申请号：US17676746

申请日：2022-02-21

Applicant: Inscripta, Inc.

Inventor： Eileen Spindler ,  Amy Hiddessen ,  Andrew Garst ,  Michael Graige ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M3/00 ,  B01L3/00 ,  C12N15/00 ,  C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US11359187B1

公开(公告)日：2022-06-14

申请号：US17680279

申请日：2022-02-24

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US20220177826A1

公开(公告)日：2022-06-09

申请号：US17676746

申请日：2022-02-21

Applicant: Inscripta, Inc.

Inventor： Eileen Spindler ,  Amy Hiddessen ,  Andrew Garst ,  Michael Graige ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M3/00 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US11345903B2

公开(公告)日：2022-05-31

申请号：US16953253

申请日：2020-11-19

Applicant: Inscripta, Inc.

Inventor： Benjamin Mijts ,  Juhan Kim ,  Aamir Mir ,  Kyle Seamon ,  Andrew Garst

IPC: C12N9/22

Abstract: The present disclosure provides engineered RNA-guided enzymes for editing live cells.

公开(公告)号：US20220145277A1

公开(公告)日：2022-05-12

申请号：US17522399

申请日：2021-11-09

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Tian Tian ,  Daniel Held

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods. Specifically, the disclosure relates to methods, compositions, modules and automated multi-module cell processing instruments that increase the efficiency of nucleic acid-guided editing in a cell population using a nucleic acid nuclease (i.e., an RNA-guided nuclease or “RGN”)/single-strand binding protein (“SSB”) fusion system. The system leverages a single-strand binding protein (SSP) and single-strand DNA annealing protein (“SSAP”) interactions to drive enhanced recruitment.

公开(公告)号：US11286471B1

公开(公告)日：2022-03-29

申请号：US17526951

申请日：2021-11-15

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US20220033855A1

公开(公告)日：2022-02-03

申请号：US17384770

申请日：2021-07-25

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Christian Siltanen

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22 ,  C12N15/63

Abstract: The present disclosure relates to methods for performing arrayed nucleic acid-guided nuclease nickase fusion editing allowing for rapid genotypic/phenotypic correlation without sequencing.