公开(公告)号：US20150175963A1

公开(公告)日：2015-06-25

申请号：US14598955

申请日：2015-01-16

Applicant: Ahmet Hoke ,  Weiran Chen

Inventor： Ahmet Hoke ,  Weiran Chen

IPC: C12N5/0793 ,  C12N9/12 ,  G01N33/50

CPC classification number: C12N5/0619 ,  C12N9/1276 ,  C12N2510/04 ,  C12N2799/027 ,  G01N33/5058 ,  G01N2500/10

Abstract: The instant invention provides methods for immortalizing cells. The invention further provides immortalized cell lines, e.g., neuronal cell lines, and methods of using these cell lines in screening assays.

Abstract translation: 本发明提供了使细胞永生化的方法。 本发明还提供永生化细胞系，例如神经元细胞系，以及在筛选测定中使用这些细胞系的方法。

公开(公告)号：US08945595B2

公开(公告)日：2015-02-03

申请号：US12398164

申请日：2009-03-04

Applicant: Anandasankar Ray ,  Stephanie Lynn Turner

Inventor： Anandasankar Ray ,  Stephanie Lynn Turner

IPC: A01N37/02 ,  A01N31/02 ,  A01N35/02

CPC classification number: G01N33/5085 ,  A01N31/02 ,  A01N35/02 ,  A01N37/02 ,  A61K49/0008 ,  G01N33/5058 ,  G01N2333/43591 ,  G01N2500/00 ,  G01N2500/10 ,  A01N2300/00

Abstract: The disclosure provides compounds useful as insect repellents and compositions comprising such repellents. The disclosure further provides insect traps.

Abstract translation: 本公开提供了可用作驱虫剂的化合物和包含该驱虫剂的组合物。 本公开进一步提供昆虫捕获器。

公开(公告)号：US20140315822A1

公开(公告)日：2014-10-23

申请号：US14356543

申请日：2012-11-07

Applicant: INSTITUT PASTEUR ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  UNVERSITE PIERRE ET MARIE CURIE

Inventor： Christophe Prehaud ,  Monique Lafon ,  Nicolas Wolff ,  Zakir Khan ,  Elouan Terrien ,  Sandrine Vitry

IPC: C07K14/435 ,  G01N33/50

CPC classification number: C12Q1/6883 ,  C07K14/005 ,  C07K14/435 ,  C07K2319/02 ,  C07K2319/05 ,  C07K2319/70 ,  C12N7/00 ,  C12N2799/027 ,  C12Q1/70 ,  C12Q2600/136 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/5023 ,  G01N33/5038 ,  G01N33/5058 ,  G01N2333/705

Abstract: The invention relates to polypeptides containing a cytoplasmic domain ending with a MAST-2 binding domain, from 11 to 13 residues, the first two residues of which are S and W, and the last four residues of which are Q, T, R and L, said polypeptides presenting a high affinity for the PDZ domain of the human MAST2 protein. The invention also relates to polynucleotides, vectors, lentiviral particles, cells as well as compositions comprising the same. The invention is also directed to the use of said polypeptides, polynucleotides, vectors, lentiviral particles, cells and compositions in the treatment and/or prevention of a disease, disorder or condition, which alters the Central Nervous System (CNS) and/or the Peripheral Nervous System (PNS). The invention also concerns molecular signatures of cellular genes to determine the neurosurvival and/or neuroprotection activity of a molecule.

Abstract translation: 本发明涉及含有以MAST-2结合域结束的胞质结构域的多肽，11至13个残基，其前两个残基为S和W，最后4个残基为Q，T，R和L 所述多肽对人MAST2蛋白的PDZ结构域具有高亲和力。 本发明还涉及多核苷酸，载体，慢病毒颗粒，细胞以及包含其的组合物。 本发明还涉及使用所述多肽，多核苷酸，载体，慢病毒颗粒，细胞和组合物来治疗和/或预防疾病，病症或病症，其改变中枢神经系统（CNS）和/或 外周神经系统（PNS）。 本发明还涉及细胞基因的分子特征，以确定分子的神经营养和/或神经保护活性。

公开(公告)号：US20140295442A1

公开(公告)日：2014-10-02

申请号：US14242402

申请日：2014-04-01

Applicant: Jason A. Hamilton ,  Anthony E. Ting ,  Robert W. Mays

Inventor： Jason A. Hamilton ,  Anthony E. Ting ,  Robert W. Mays

IPC: G01N33/53 ,  C12Q1/68

CPC classification number: G01N33/5058 ,  A61K35/28 ,  C12Q1/68 ,  G01N33/5005 ,  G01N33/5023 ,  G01N33/53 ,  G01N2500/04 ,  G01N2500/10

Abstract: The invention provides methods for treating pathological conditions associated with an undesirable inflammatory component. The invention is generally directed to reducing inflammation by administering cells that modulate microglia activation. The invention is also directed to drug discovery methods to screen for agents that modulate the ability of the cells to modulate microglia activation. The invention is also directed to cell banks that can be used to provide cells for administration to a subject, the banks comprising cells having desired levels of potency to modulate microglia activation.

Abstract translation: 本发明提供了治疗与不良炎症成分相关的病理状况的方法。 本发明一般涉及通过施用调节小胶质细胞活化的细胞来减少炎症。 本发明还涉及用于筛选调节细胞调节小胶质细胞活化能力的试剂的药物发现方法。 本发明还涉及可用于提供用于给予受试者的细胞的细胞库，所述细胞库包含具有期望水平的效力以调节小神经胶质细胞活化的细胞。

公开(公告)号：US20140294726A1

公开(公告)日：2014-10-02

申请号：US14233606

申请日：2012-07-18

Applicant: Wilfred Jefferies ,  Kaan E. Biron ,  Dara L. Dickstein

Inventor： Wilfred Jefferies ,  Kaan E. Biron ,  Dara L. Dickstein

IPC: A61K38/21 ,  A61K31/506 ,  A61K38/17 ,  G01N33/50 ,  A61K38/20 ,  A61K31/436 ,  C07K16/18 ,  C07K16/22 ,  A61K31/05

CPC classification number: A61K39/0007 ,  A61K31/05 ,  A61K31/192 ,  A61K31/404 ,  A61K31/42 ,  A61K31/436 ,  A61K31/44 ,  A61K31/506 ,  A61K31/551 ,  A61K38/1709 ,  A61K38/195 ,  A61K38/208 ,  A61K38/21 ,  A61K38/212 ,  A61K38/39 ,  A61K38/484 ,  A61K2039/55566 ,  A61K2039/575 ,  C07K16/18 ,  C07K16/22 ,  G01N33/5058

Abstract: Methods for diagnosing and treating Alzheimer's disease are provided. In particular, methods of restoring blood-brain barrier integrity and/or promoting vascular reversion in a person suffering from Alzheimer's disease by administering an anti-angiogenic agent or an agent that is capable of restoring tight junction integrity. Methods of preventing or delaying the onset of Alzheimer's disease in a subject are also provided.

Abstract translation: 提供了诊断和治疗阿尔茨海默氏病的方法。 特别地，通过施用抗血管生成剂或能够恢复紧密连接完整性的药剂，恢复患有阿尔茨海默氏病的人的血脑屏障完整性和/或促进血管逆转的方法。 还提供了预防或延迟受试者中阿尔茨海默病发作的方法。

公开(公告)号：US08809052B2

公开(公告)日：2014-08-19

申请号：US12310495

申请日：2007-08-15

Applicant: Michel Revel ,  Judith Chebath ,  Michal Izrael ,  Rosalia Kaufman

Inventor： Michel Revel ,  Judith Chebath ,  Michal Izrael ,  Rosalia Kaufman

IPC: C12N5/071 ,  C12N5/00

CPC classification number: C12N5/0622 ,  A61K35/30 ,  C12N5/0619 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2506/02 ,  C12N2533/52 ,  C12N2533/90 ,  G01N33/5058

Abstract: A method of generating neural and glial cells is provided. The method comprising growing human stem cells under conditions which induce differentiation of said human stem cells into the neural and glial cells, said conditions comprising the presence of retinoic acid and an agent capable of down-regulating Bone Morphogenic Protein activity.

Abstract translation: 提供了一种产生神经和神经胶质细胞的方法。 该方法包括在诱导所述人类干细胞分化为神经和神经胶质细胞的条件下培养人类干细胞，所述条件包括视黄酸和能够下调骨形态发生蛋白活性的试剂的存在。

公开(公告)号：US20140178305A1

公开(公告)日：2014-06-26

申请号：US14135102

申请日：2013-12-19

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： David J. Anderson ,  Sophia Vrontou

IPC: A61K49/00 ,  G01N33/50

CPC classification number: G01N33/5058 ,  A61K49/0008

Abstract: The present disclosure provides methods and compositions that can be used to identify anxiolytic compounds, and to treat anxiety in a subject. Methods and compositions for identifying activating stimuli for sensory neurons, for example MrgprB4+ neurons, are also disclosed herein.

Abstract translation: 本公开提供可用于鉴定抗焦虑化合物并治疗受试者焦虑症的方法和组合物。 用于识别感觉神经元的激活刺激的方法和组合物，例如MrgprB4 +神经元，也在本文中公开。

公开(公告)号：US20140171380A1

公开(公告)日：2014-06-19

申请号：US14020776

申请日：2013-09-06

Applicant: The Trustees of Columbia University in the City of New York

Inventor： Tae-Wan Kim ,  Laura Beth Johnson McIntire ,  Natalie Landman ,  Gina Finan

IPC: G01N33/569 ,  A61K31/01 ,  A61K31/145 ,  A61K31/5375 ,  A61K31/15 ,  A61K31/7048 ,  A61K31/473 ,  A61K31/137 ,  A61K31/41

CPC classification number: G01N33/56966 ,  A61K31/01 ,  A61K31/03 ,  A61K31/085 ,  A61K31/137 ,  A61K31/145 ,  A61K31/15 ,  A61K31/41 ,  A61K31/473 ,  A61K31/5375 ,  A61K31/7048 ,  C12N5/0619 ,  C12N2501/385 ,  C12N2506/02 ,  C12N2533/50 ,  C12N2533/52 ,  G01N33/5058 ,  G01N2800/2821

Abstract: Successful CNS drug discovery requires a scalable, highly physiological neuronal model. Using directed differentiation of mouse embryonic stem (mES) cells, including mES cells isolated from a mouse model of Alzheimer's disease (AD), a highly homogeneous primary neuronal model amenable to phenotypic assays for production and synaptotoxicity of amyloid β-peptide was developed. This model furnishes a highly physiological and AD-relevant platform suitable for high throughput small molecule and functional genetic screens, providing specific small molecule compounds identified by such screens.

Abstract translation: 成功的CNS药物发现需要可扩展的，高度生理的神经元模型。 使用小鼠胚胎干（mES）细胞的定向分化，包括从阿尔茨海默氏病（AD）的小鼠模型分离的mES细胞，开发了适合淀粉样蛋白和生物肽的生产表型测定和突触毒性的高度均一的原代神经元模型。 该模型提供高生理和AD相关平台，适用于高通量小分子和功能遗传筛选，提供通过这种筛选鉴定的特异性小分子化合物。

公开(公告)号：US20140161777A1

公开(公告)日：2014-06-12

申请号：US14180129

申请日：2014-02-13

Applicant: University of Iowa Research Foundation

Inventor： Beverly L. Davidson ,  Yong Hong Chen

IPC: C07K7/06 ,  C07K5/10 ,  C07K5/08

CPC classification number: A61K38/162 ,  A61K48/00 ,  C07K5/08 ,  C07K5/10 ,  C07K7/06 ,  C07K14/005 ,  C07K2319/33 ,  C12N15/86 ,  C12N2750/14133 ,  C12N2750/14143 ,  C12N2750/14145 ,  C12N2810/40 ,  G01N33/5058

Abstract: The present disclosure provides targeting peptides and vectors containing a sequence that encodes targeting peptides that deliver agents to the brain.

Abstract translation: 本公开提供了靶向肽和载体，其含有编码将药物递送至脑的靶向肽的序列。

公开(公告)号：US20140105880A1

公开(公告)日：2014-04-17

申请号：US13969280

申请日：2013-08-16

Applicant: biOasis Advanced Technologies Inc.

Inventor： Christopher M. Starr ,  Todd Zankel

IPC: A61K47/48 ,  A61K38/47 ,  A61K38/46

CPC classification number: A61K47/64 ,  A61K38/465 ,  A61K38/47 ,  C12Y302/01076 ,  G01N33/5008 ,  G01N33/5035 ,  G01N33/5058 ,  G01N33/566 ,  A61K2300/00

Abstract: The present invention provides for compositions and methods for treating, ameliorating or preventing a lysosomal storage disease by administering to a patient suffering from a lysosomal storage disease a P97 conjugated with an enzyme which is capable of transportation into the lysosomes of cells on either sides of the blood brain harrier.

Abstract translation: 本发明提供了通过向患有溶酶体储存疾病的患者施用与能够转运到细胞的溶酶体中的P97缀合的P97来治疗，改善或预防溶酶体贮积病的组合物和方法 血脑梗