公开(公告)号：US06875573B2

公开(公告)日：2005-04-05

申请号：US10190967

申请日：2002-07-08

申请人： Carl Fuller ,  Joseph Szasz

发明人： Carl Fuller ,  Joseph Szasz

IPC分类号： C12N9/12 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N9/1252

摘要： Thermostable DNA polymerases both in native form and having single amino acid substitutions and optionally N-terminal deletions are disclosed. These polymerases exhibit a substantial improvement of DNA sequencing performance compared to Taq DNA polymerase. The instant DNA polymerases also possess improved salt tolerance.

摘要翻译： 公开了天然形式和具有单个氨基酸取代和任选的N-末端缺失的热稳定的DNA聚合酶。 与Taq DNA聚合酶相比，这些聚合酶显示出DNA测序性能的显着改善。 即时DNA聚合酶也具有改善的耐盐性。

公开(公告)号：US06967250B1

公开(公告)日：2005-11-22

申请号：US09386576

申请日：1999-08-30

申请人： Shiv Kumar ,  Satyam Nampalli ,  Mahesh Khot

发明人： Shiv Kumar ,  Satyam Nampalli ,  Mahesh Khot

IPC分类号： C07D265/36 ,  G01N33/542

CPC分类号： G01N33/542

摘要： Energy transfer dyes, their preparation, and their use as labels in biological systems is disclosed. The dyes are preferably in the form of cassettes which enable their attachment to a variety of biological materials. The dyes and the reagents that can be made from them offer a wide variety of fluorescent labels with large Stokes' shifts enabling their use in a variety of fluorescence applications over a wide range of the visible spectrum.

摘要翻译： 公开了能量转移染料，它们的制备及其作为生物系统中的标记物的用途。 染料优选为使其能够附着于各种生物材料的盒的形式。 可以由其制成的染料和试剂提供了广泛的荧光标记，具有大的斯托克斯变化，使其能够在广泛的可见光谱范围内用于各种荧光应用。

公开(公告)号：US06906185B1

公开(公告)日：2005-06-14

申请号：US09045732

申请日：1998-03-20

申请人： Carl W. Fuller ,  Mark McDougall ,  Shiv Kumar

发明人： Carl W. Fuller ,  Mark McDougall ,  Shiv Kumar

IPC分类号： C07H19/173 ,  C07H21/04 ,  C12P19/32

CPC分类号： C07H19/173 ,  C07H21/04

摘要： Derivatives of 7-deaza-2′-deoxyguanosine are described. Also described is the use of such compounds in synthesis of nucleic acid polymers and in methods for determining a nucleotide base sequence.

摘要翻译： 描述了7-脱氮-2'-脱氧鸟苷的衍生物。 还描述了这样的化合物在核酸聚合物的合成中的用途以及用于测定核苷酸碱基序列的方法。

公开(公告)号：US07041812B2

公开(公告)日：2006-05-09

申请号：US10230576

申请日：2002-08-29

申请人： Shiv Kumar ,  Anup Sood

发明人： Shiv Kumar ,  Anup Sood

IPC分类号： C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/6851

摘要： The present invention describes new compositions of matter in the form of labeled nucleoside polyphosphates with four or more phosphates. In addition compositions of nucleoside polyphosphates with four or more phosphates that are substrates for nucleic acid polymerases with enhanced substrate properties and methods of using these nucleoside polyphosphates for nucleic acid detection, characterization and quantification are described. The compositions provided by this invention include nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogues which have colorimetric, chemiluminescent, or fluorescent moieties, mass tags or an electrochemical tags attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Removal of the polyphosphate product of phosphoryl transfer via phosphate or polyphosphate transferring enzyme leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

摘要翻译： 本发明描述了具有四种或更多种磷酸盐的标记的核苷多磷酸盐形式的物质的新组合物。 此外，还描述了具有四种或更多种磷酸盐的核苷多磷酸盐的组合物，其是具有增强的底物性质的核酸聚合酶的底物和使用这些核苷多磷酸盐用于核酸检测，表征和定量的方法。 本发明提供的组合物包括具有比色，化学发光或荧光部分的核苷多磷酸，双脱氧核苷多磷酸或脱氧核苷多磷酸酯类似物，质粒标签或连接至末端磷酸的电化学标记。 当核酸聚合酶使用该类似物作为底物时，酶活性标记将存在于磷酸转移的无机多磷酸盐副产物上。 通过磷酸盐或多磷酸盐转移酶去除磷酸转移的多磷酸盐产物导致附着在其上的标签的可检测的变化。 当在磷酸酶的存在下进行聚合酶测定时，提供了用于实时监测DNA或RNA合成和检测靶核酸的方便的方法。

公开(公告)号：US06949635B1

公开(公告)日：2005-09-27

申请号：US09699030

申请日：2000-10-26

申请人： Shiy Kumar ,  Satyam Nampalli ,  Bernard F. McArdle ,  Carl W. Fuller

发明人： Shiy Kumar ,  Satyam Nampalli ,  Bernard F. McArdle ,  Carl W. Fuller

IPC分类号： C12N15/09 ,  C07F9/6558 ,  C07H19/10 ,  C07H19/20 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/68 ,  C07H21/02

CPC分类号： C07H19/10 ,  C07H19/20 ,  C12Q1/6869 ,  C12Q2525/186

摘要： A kit for DNA sequencing comprising a first, second, third and fourth dye terminator molecules, each of the dye terminator molecules comprising a dye molecule, a linker of at least 10 atoms in length and either ddATP, ddCTP, ddGTP or ddTTP as a mono or tri-phosphate and a thermostable DNA polymerase.

摘要翻译： 用于DNA测序的试剂盒包括第一，第二，第三和第四染料终止子分子，每个染料终止子分子包含染料分子，长度至少为10个原子的接头和ddATP，ddCTP，ddGTP或ddTTP作为单 或三磷酸盐和热稳定的DNA聚合酶。

公开(公告)号：US06902657B2

公开(公告)日：2005-06-07

申请号：US09882523

申请日：2001-06-15

申请人： Marek Minarik ,  Melanie M. Mahtani

发明人： Marek Minarik ,  Melanie M. Mahtani

IPC分类号： G01N27/62 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/64 ,  G01N27/447 ,  G01N37/00 ,  B01D57/02

CPC分类号： G01N27/44747

摘要： A low molecular weight non-entangled polyvinylpyrrolidone for use as a separation media for microchannel electrophoretic separation. The separation media may be used in a system in which multiple samples of small compounds are injected into a single microchannel at time spaced intervals followed by a continuous detection interval.

摘要翻译： 用作微通道电泳分离分离介质的低分子量非缠结聚乙烯吡咯烷酮。 分离介质可以用于将多个小化合物样品以间隔间隔注入单个微通道并随后连续检测间隔的系统中。

公开(公告)号：US07052839B2

公开(公告)日：2006-05-30

申请号：US10113030

申请日：2002-04-01

申请人： John Nelson ,  Carl Fuller ,  Anup Sood ,  Shiv Kumar

发明人： John Nelson ,  Carl Fuller ,  Anup Sood ,  Shiv Kumar

IPC分类号： C12Q1/68

CPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6851

摘要： The present invention describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

公开(公告)号：US20050095640A1

公开(公告)日：2005-05-05

申请号：US11013179

申请日：2004-12-15

申请人： Hrissi Samartzidou ,  Leah Turner ,  Steven Daniel ,  Thomas Houts

发明人： Hrissi Samartzidou ,  Leah Turner ,  Steven Daniel ,  Thomas Houts

IPC分类号： C12N15/09 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6809 ,  C12Q2545/101

摘要： Method of producing controls for use in gene expression analysis systems such as macroarrays, real-time PCR, northern blots, SAGE and microarrays. The controls are generated either from near-random sequence of DNA, or from inter- or intragenic regions of a genome. Ten specific control sequences are also disclosed. Also presented are methods of using these controls, including as negative controls, positive controls, and as calibrators of a gene expression analysis system.

公开(公告)号：US07033762B2

公开(公告)日：2006-04-25

申请号：US10113025

申请日：2002-04-01

申请人： John Nelson ,  Carl Fuller ,  Anup Sood ,  Shiv Kumar

发明人： John Nelson ,  Carl Fuller ,  Anup Sood ,  Shiv Kumar

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6823 ,  C12Q2545/114 ,  C12Q2521/525 ,  C12Q2521/101

摘要： A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, a non-hydrolyzable primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and an enzyme having 3′→5′ exonuclease activity which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species characteristic of the sample; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on the detection.

公开(公告)号：US20060035360A1

公开(公告)日：2006-02-16

申请号：US11242730

申请日：2005-10-04

申请人： Joseph Farchaus

发明人： Joseph Farchaus

IPC分类号： C12N9/22 ,  C12P21/06 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N9/1252

摘要： The present invention relates to methods and compositions for providing purified thermostable enzymes, particularly thermostable DNA polymerases, that are free of exogenous detergents. The present invention also provides methods for providing such purified thermostable DNA polymerases to assays in an active form by adding one or more detergents. The present invention further provides compositions and kits comprising purified thermostable DNA polymerases for use in a variety of applications, including amplification and sequencing of nucleic acids.