公开(公告)号：US10456433B2

公开(公告)日：2019-10-29

申请号：US15563993

申请日：2016-04-07

申请人： euglena Co., Ltd.

发明人： Ayaka Nakashima ,  Yuta Asayama ,  Osamu Iwata ,  Kengo Suzuki

IPC分类号： A61K36/05 ,  A61K35/68 ,  A23L33/10 ,  A61P19/02 ,  A61K45/06

摘要： Provided are an inhibitor, a prophylactic, and a therapeutic agent for rheumatoid arthritis that have no side effects and that can correct immune abnormality associated with rheumatoid arthritis. The inhibitor, the prophylactic, and the agent for rheumatoid arthritis contain a Euglena-derived material as an active ingredient. The inhibitor, the prophylactic and the agent are used as an antirheumatic drug for controlling the activity of rheumatoid arthritis by alleviating immune abnormality associated with rheumatoid arthritis. The inhibitor, the prophylactic, and the agent are also used for a living individual who has not been definitively diagnosed with rheumatoid arthritis. The inhibitor, the prophylactic, and the agent are continuously administered to the living individual from any point before definitive diagnosis of rheumatoid arthritis to any point after the definitive diagnosis. The Euglena-derived material is at least one material selected from the group including Euglena algae, paramylon derived from the Euglena, water-soluble amorphous paramylon prepared by treating the paramylon with alkali and neutralizing the treated paramylon, and emulsion paramylon prepared by ejecting a solution of the paramylon from a narrow bore nozzle at a very high pressure to cause the solution to collide with a collision surface.

公开(公告)号：US20160281077A1

公开(公告)日：2016-09-29

申请号：US15080766

申请日：2016-03-25

申请人： Euglena Co., Ltd.

发明人： Akihiro OKAMOTO ,  Kazuhito HASHIMOTO ,  Yuki TOSHIMA ,  Shuji NAKANISHI ,  Iwao UEDA ,  Nobuo AOKI ,  Kengo SUZUKI

IPC分类号： C12N13/00 ,  C12N1/12

CPC分类号： C12N13/00 ,  C12N1/12 ,  Y02E50/343

摘要： A medium for culturing Euglena includes a cell membrane-permeable electron mediator that includes a biocompatible moiety and a redox-active moiety. A method for culturing Euglena includes culturing Euglena in a medium including a cell membrane-permeable electron mediator that includes a biocompatible moiety and a redox-active moiety. The medium is placed in a fermenter system that includes a device for extracting electrons from the medium. A method for reducing photoinhibition in Euglena includes electrochemically culturing Euglena in a medium including a cell membrane-permeable electron mediator that includes a biocompatible moiety and a redox-active moiety. The medium is placed in a fermenter system that includes a device for extracting electrons from the medium.

摘要翻译： 培养Euglena的培养基包括细胞膜可渗透的电子介体，其包括生物相容性部分和氧化还原活性部分。 培养金丝雀菌的方法包括在包括生物相容性部分和氧化还原活性部分的细胞膜可渗透的电子介体的培养基中培养Euglena。 将介质置于包括用于从介质中提取电子的装置的发酵罐系统中。 减少Euglena光抑制的方法包括在包括生物相容性部分和氧化还原活性部分的细胞膜可渗透电子介体的培养基中电化学培养Euglena。 将介质置于包括用于从介质中提取电子的装置的发酵罐系统中。

公开(公告)号：US08951773B2

公开(公告)日：2015-02-10

申请号：US13699780

申请日：2011-05-25

申请人： Hiroaki Kato ,  Ko Yamashita ,  Yukio Fukushima ,  Ken Amano ,  Takashi Kaneko ,  Iwao Ueda ,  Nobuo Aoki ,  Kengo Suzuki ,  Ryo Arashida ,  Ryohei Nakano

发明人： Hiroaki Kato ,  Ko Yamashita ,  Yukio Fukushima ,  Ken Amano ,  Takashi Kaneko ,  Iwao Ueda ,  Nobuo Aoki ,  Kengo Suzuki ,  Ryo Arashida ,  Ryohei Nakano

IPC分类号： C12N1/00 ,  C12N1/12 ,  C12P7/64 ,  C10L1/02

CPC分类号： C12P7/64 ,  C10G2300/1014 ,  C10G2300/4043 ,  C10L1/02 ,  C11B1/10 ,  C11C3/00 ,  C12P7/6463 ,  C12P7/649 ,  Y02E50/13 ,  Y02P30/20 ,  Y02P30/446

摘要： The present invention provides a production method for biofuel based on a technology to convert carbon-dioxide as a carbon source through photosynthesis by photosynthetic microorganisms to biomass and produce biofuel of the biomass. The production method for biofuel of the present invention comprises a culturing process (S1) of culturing in a culture solution photosynthetic microorganisms which store oils, fats and carbohydrates in cells of the photosynthetic microorganisms, an oil and fat conversion process (S2) of converting the carbohydrates stored in the cells of the photosynthetic microorganisms cultured in the culture apparatus to oils and fats, an extraction process (S3) of extracting the oils and fats out of the cells of the photosynthetic microorganisms, and a reforming process (S4) to reform the extracted oils and fats.

摘要翻译： 本发明提供了一种生物燃料的生产方法，其基于通过光合微生物通过光合作用将碳二
氧化碳转化为生物质并生产生物燃料的技术。 本发明的生物燃料的制造方法包括在光合微生物的细胞中储存油，脂肪和碳水化合物的培养液中培养光合微生物的培养方法（S1），将光合微生物的细胞中的油脂转化方法（S2） 储存在培养装置中培养的光合微生物的细胞中的碳水化合物用于油和脂肪，从光合微生物的细胞中提取油和脂肪的提取方法（S3）和重整方法（S4） 提取油脂。

公开(公告)号：US10047338B2

公开(公告)日：2018-08-14

申请号：US15304601

申请日：2015-04-16

申请人： euglena Co., Ltd. ,  SHIMIZU CORPORATION

发明人： Kengo Suzuki ,  Ryohei Nakano ,  Hideyuki Adachi ,  Ayaka Nakashima ,  Eriko Yoshida ,  Masaharu Tasaki ,  Yoichi Kuroiwa ,  Keisuke Kojima ,  Takeshi Hasegawa

IPC分类号： C12P13/04 ,  C12N1/12 ,  C12N1/20 ,  C12P19/44 ,  C12P13/00

摘要： An algae culture method capable of efficiently producing an osmotic pressure adjusting substance, a production method of the substance, and an algae culture method for recovering carbon dioxide from a mixed gas containing carbon dioxide and sulfurous acid gas. The methods involve preparing a plurality of enrichment cultures each containing betaine by culturing a culture of microalgae derived from an environmental specimen under a photoautotrophic condition and under a plurality of culture conditions; making a cultivation plan in which an optimum enrichment culture suitable for a main culture is selected from the plurality of enrichment cultures; producing a main culture that contains betaine under the photoautotrophic condition and a salt concentration of 10 wt. % or more; and separating betaine. In the main culture, the algae containing betaine are cultured while the mixed gas containing sulfurous acid gas and carbon dioxide is blown to a culture solution of the algae.

公开(公告)号：US20180112175A1

公开(公告)日：2018-04-26

申请号：US15571280

申请日：2016-05-06

申请人： Riken ,  eugiena Co,. Ltd.

发明人： Takashi Osanai ,  Masami Hirai ,  Hiroko IIjima ,  Yuka Nakaya ,  Kengo Suzuki ,  Osamu Iwata ,  Ayaka Nakashima

IPC分类号： C12N1/10 ,  C12P7/46

CPC分类号： C12N1/10 ,  C12P7/40 ,  C12P7/46

摘要： The present invention provides a method for producing an organic acid using algal biomass, The present invention pertains to the production of organic acids such as succinic acid using Euglena , by means of a method containing; either a nitrogen deficient culture step in which Euglena is cultured. aerobically under nitrogen-deficient conditions or a heterotrophic culture step in which Euglena is cultured aerobically using a culture medium containing a carbon source; and an anaerobic culture step in which the culture product obtained in the nitrogen-deficient culture step is incubated under anaerobic conditions.

公开(公告)号：US20170020939A1

公开(公告)日：2017-01-26

申请号：US15302300

申请日：2015-04-08

申请人： euglena Co., Ltd.

发明人： Ayaka NAKASHIMA ,  Yuta ASAYAMA ,  Eriko YOSHIDA ,  Osamu IWATA ,  Kengo SUZUKI

IPC分类号： A61K36/02 ,  A23L33/10 ,  A61K31/715

CPC分类号： A61K36/02 ,  A23L33/10 ,  A23L33/105 ,  A23V2002/00 ,  A61K31/715 ,  A61K31/716 ,  C12N1/10

摘要： The present invention provides a novel immune balance regulator that regulates an immune balance in a living body. The immune balance regulator contains a Euglena-derived material and regulates an immune balance between Th1, Th2, and Th17, which is a balance between immune responses individually induced by Th1, Th2, and Th17 in a living body. The immune balance regulator regulates the immune balance between Th1, Th2, and Th17 so that immune responses induced by Th1 become relatively dominant over immune responses induced by Th2 or Th17 to improve a physical constitution that has an immune imbalance of Th1, Th2, and Th17 shifted towards Th2 and to prevent or treat a disease associated with the immune imbalance of Th1, Th2, and Th17 shifted towards a Th2 dominance. The immune balance regulator is administered prior to expected onset of a disease associated with the immune imbalance of Th1, Th2, and Th17 shifted towards the Th2 dominance.

摘要翻译： 本发明提供了调节活体免疫平衡的新型免疫平衡调节剂。 免疫平衡调节物含有来自Euglena的材料并调节Th1，Th2和Th17之间的免疫平衡，Th1，Th2和Th17是活体中Th1，Th2和Th17单独诱导的免疫反应之间的平衡。 免疫平衡调节因子调节Th1，Th2和Th17之间的免疫平衡，使Th1诱导的免疫反应相对于由Th2或Th17诱导的免疫应答相对优势，以改善具有Th1，Th2和Th17免疫失衡的物理结构 转移到Th2，并预防或治疗与Th1，Th2和Th17的免疫不平衡相关的疾病向Th2优势转移。 免疫平衡调节剂在预期发生与Th1，Th2和Th17的免疫失衡相关的疾病之前施用，向Th2优势移动。

公开(公告)号：US20180133271A1

公开(公告)日：2018-05-17

申请号：US15563993

申请日：2016-04-07

申请人： euglena Co., Ltd.

发明人： Ayaka NAKASHIMA ,  Yuta ASAYAMA ,  Osamu IWATA ,  Kengo SUZUKI

IPC分类号： A61K36/05 ,  A23L33/10 ,  A61P19/02

摘要： Provided are an inhibitor, a prophylactic, and a therapeutic agent for rheumatoid arthritis that have no side effects and that can correct immune abnormality associated with rheumatoid arthritis. The inhibitor, the prophylactic, and the agent for rheumatoid arthritis contain a Euglena-derived material as an active ingredient. The inhibitor, the prophylactic and the agent are used as an antirheumatic drug for controlling the activity of rheumatoid arthritis by alleviating immune abnormality associated with rheumatoid arthritis. The inhibitor, the prophylactic, and the agent are also used for a living individual who has not been definitively diagnosed with rheumatoid arthritis. The inhibitor, the prophylactic, and the agent are continuously administered to the living individual from any point before definitive diagnosis of rheumatoid arthritis to any point after the definitive diagnosis. The Euglena-derived material is at least one material selected from the group including Euglena algae, paramylon derived from the Euglena, water-soluble amorphous paramylon prepared by treating the paramylon with alkali and neutralizing the treated paramylon, and emulsion paramylon prepared by ejecting a solution of the paramylon from a narrow bore nozzle at a very high pressure to cause the solution to collide with a collision surface.

公开(公告)号：US20170319632A1

公开(公告)日：2017-11-09

申请号：US15524522

申请日：2015-11-06

申请人： euglena Co., Ltd.

发明人： Eriko YOSHIDA ,  Yuta ASAYAMA ,  Osamu IWATA ,  Ayaka NAKASHIMA ,  Kengo SUZUKI ,  Misa OHGUSHI ,  Naoki HARADA ,  Yoshihisa NAKANO

IPC分类号： A61K35/68 ,  A61K31/716

CPC分类号： A61K35/68 ,  A61K31/716

摘要： Provided are a prophylactic or therapeutic agent for a peptic ulcer, a food additive for prevention and treatment of a peptic ulcer, an iNOS expression inhibitor, and a COX-2 expression inhibitor, all of which have little side effects. The prophylactic or therapeutic agent contains a Euglena-derived material as an active ingredient. The Euglena-derived material may be Euglena, paramylon, or an acid-treated paramylon. The prophylactic or therapeutic agent is useful for the prevention or treatment of a peptic ulcer induced by psychological stress. The peptic ulcer may be a gastric ulcer. The food additive contains a Euglena-derived material as an active ingredient.

公开(公告)号：US09644193B2

公开(公告)日：2017-05-09

申请号：US14909211

申请日：2014-08-04

申请人： Euglena Co., Ltd.

发明人： Kengo Suzuki ,  Ryo Arashida ,  Yuka Marukawa ,  Eriko Yoshida ,  Takumi Takeda ,  Yuki Nakano ,  Naotake Konno ,  Machiko Takahashi

IPC分类号： C12N9/26 ,  C12N15/00 ,  C12P19/14 ,  C12P21/06 ,  C12P21/04 ,  C07H21/04 ,  C12N9/42 ,  C12N9/24 ,  A23K20/189 ,  C12P19/12

CPC分类号： C12N9/244 ,  A23K20/189 ,  C12N9/2405 ,  C12P19/12 ,  C12P19/14 ,  C12Y302/01006 ,  C12Y302/01039

摘要： A β-1,3-glucanase that exhibits decomposition activity with respect to paramylon derived from the genus Euglena. The β-1,3-glucanase is derived from the genus Euglena and exhibits the properties indicated below: (1) effect: hydrolyzing the β-1,3-bond of β-1,3-glucan; and (2) substrate specificity: decomposing at least paramylon. The β-1,3-glucanase additionally exhibits the properties indicated below: (3) decomposition activity: the ratio of paramylon decomposition activity with respect to laminarin decomposition activity is 20% or higher; (4) optimum pH: 3.7-7.0; and (5) optimum temperature: 30-70° C.

公开(公告)号：US20160010070A1

公开(公告)日：2016-01-14

申请号：US14770715

申请日：2014-02-28

申请人： EUGLENA CO., LTD. ,  KINKI UNIVERSITY

发明人： Shigeru SHIGEOKA ,  Masahiro TAMOI ,  Kengo SUZUKI ,  Eriko YOSHIDA

IPC分类号： C12N9/16 ,  C12N15/79

CPC分类号： C12N9/16 ,  C12N15/79 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8245 ,  C12N15/8246 ,  C12N15/825 ,  C12N15/8261 ,  C12N15/8269 ,  C12N15/895 ,  C12Y301/03011 ,  C12Y301/03037 ,  Y02A40/146

摘要： The present invention provides a method for introducing a gene into Euglena, which can stably maintain a foreign gene, and a transformant therefrom. In this method of introducing a gene into Euglena, a DNA fragment containing an amino acid sequence for encoding a protein is introduced into a Euglena cell. The method includes a step of producing a binary vector containing a DNA fragment, a step of obtaining a linear gene fragment that includes a T-DNA region including the DNA fragment in the binary vector, and a direct gene introduction step of directly introducing the linear gene fragment into the Euglena cell.

摘要翻译： 本发明提供了将基因引入到可以稳定地维持外源基因的金丝雀中的方法及其转化体。 在将基因引入欧氏细胞膜的方法中，将含有用于编码蛋白质的氨基酸序列的DNA片段引入欧格琳娜细胞。 该方法包括制备含有DNA片段的二元载体的步骤，获得包含二元载体中包含DNA片段的T-DNA区的线性基因片段的步骤和直接引入线性的直接基因导入步骤 基因片段进入Euglena细胞。