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公开(公告)号：US06964869B2
公开(公告)日：2005-11-15
申请号：US10131977
申请日：2002-04-24
CPC分类号： C12N5/0698 , A61K35/12 , C12N5/0629 , C12N2502/094 , C12N2502/1323 , C12N2502/30 , C12N2502/99 , C12N2503/02 , C12N2503/06 , C12N2510/00 , C12N2510/04 , G01N33/5014 , G01N33/5082 , G01N2500/10
摘要： A chimeric skin comprising immortalized human keratinocyte cells cocultured with donor keratinocytes is disclosed.
摘要翻译： 公开了包含与供体角质形成细胞共培养的永生化人角质形成细胞的嵌合皮肤。
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公开(公告)号：US06514711B2
公开(公告)日：2003-02-04
申请号：US09945136
申请日：2001-08-31
IPC分类号： G01N33567
CPC分类号： C12N5/0629 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2502/30 , C12N2503/02 , C12N2503/06 , C12N2510/00 , C12N2510/04 , G01N33/5014 , G01N2500/10
摘要： A method for testing the effects of various factors on human skin equivalent is disclosed. The method involves providing a human stratified squamous epithelial cell culture of an immortalized human keratinocyte cell line that forms a reconstructed epidermis, exposing the reconstructed epidermis to a factor and evaluating the effect of the factor on the reconstructed epidermis. A method for selecting preventive or therapeutic agents for skin damages caused by a factor using the same human stratified squamous epithelial cell culture system is also disclosed.
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公开(公告)号：US06214567B1
公开(公告)日：2001-04-10
申请号：US09277295
申请日：1999-03-26
IPC分类号： G01N33567
CPC分类号： C12N5/0629 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2502/30 , C12N2503/02 , C12N2503/06 , C12N2510/00 , C12N2510/04 , G01N33/5014 , G01N2500/10
摘要： A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. The method comprises the steps of obtaining a human stratified squamous epithelial cell culture, wherein the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, wherein the culture forms a reconstituted epidermis. One then treats the epidermis with a test tumor cell modulation agent and evaluates the growth of the malignant cells within the epidermis.
摘要翻译： 公开了一种自发永生化的人角质形成细胞系。 在优选的实施方案中，该细胞系是ATCC 12191.在本发明的另一个实施方案中，公开了测定肿瘤细胞调节剂的作用的方法。 该方法包括获得人分层鳞状上皮细胞培养物的步骤，其中培养物包含人恶性鳞状上皮细胞和自发永生化人角质形成细胞，其中培养物形成重构表皮。 然后用一种测试的肿瘤细胞调节剂治疗表皮，并评估表皮内恶性细胞的生长。
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公开(公告)号：US09295543B2
公开(公告)日：2016-03-29
申请号：US11375950
申请日：2006-03-15
CPC分类号： A61F2/105 , C12N5/0629 , C12N5/0698 , C12N2502/094 , C12N2502/1323
摘要： The present invention relates to in vitro cultured skin substitutes. In particular, the present invention relates to compositions and methods for the development of cultured skin substitutes using NIKS cells.
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公开(公告)号：US08790636B2
公开(公告)日：2014-07-29
申请号：US13343314
申请日：2012-01-04
CPC分类号： A61L27/60 , A61K35/12 , A61K35/36 , A61K38/1709 , A61K38/1825 , A61K2035/122 , A61L27/227 , A61L27/3813 , A61L27/3895 , A61L27/54 , A61L2300/252 , A61L2300/404 , C07H21/04 , C07K14/50 , C12N5/0629 , C12N5/0698 , C12N15/63 , C12N15/79 , C12N2501/117 , C12N2501/998 , C12N2502/094 , C12N2502/1323 , C12N2510/00
摘要： The present invention relates generally to compositions for wound closure. More specifically, the present invention provides human skin equivalents engineered to express exogenous polypeptides (e.g., antimicrobial polypeptides and keratinocyte growth factor 2) and compositions and methods for making human skin equivalents engineered to express exogenous polypeptides. In addition, the present invention provides methods for treatment of wounds with human skin equivalents engineered to express exogenous polypeptides.
摘要翻译： 本发明一般涉及用于伤口闭合的组合物。 更具体地，本发明提供了用于表达外源多肽（例如抗微生物多肽和角质形成细胞生长因子2）的人皮肤当量以及用于制造人皮肤等同物的工程化以表达外源多肽的组合物和方法。 此外，本发明提供了用于表达外源多肽的用于治疗具有人皮肤当量的伤口的伤口的方法。
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公开(公告)号：US08580314B2
公开(公告)日：2013-11-12
申请号：US12612284
申请日：2009-11-04
IPC分类号： A61K35/12
CPC分类号： A61L27/3691 , A61F2/105 , A61L27/3813 , A61L27/54 , A61L27/60 , A61L2430/40
摘要： The present invention relates generally to systems and methods for preparing, storing, shipping and using skin equivalents made by organotypic culture. In particular, the present invention relates to systems and methods for producing, transporting, storing and using skin equivalents produced by organotypic culture at reduced temperatures, preferably from 2-8 degrees Celsius to ambient temperature. The methods include sterile packaging of the grafts so that the sterility and integrity of the package is maintained until the time of use for grafting purposes.
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公开(公告)号：US07888496B2
公开(公告)日：2011-02-15
申请号：US12329151
申请日：2008-12-05
IPC分类号： C07H21/04
CPC分类号： C12Q1/6888 , C12Q2600/16 , Y10S435/81
摘要： The present invention relates to methods and compositions for the identification of species-specific material in pharmaceutical products. In particular, the present invention relates to methods for the identification of species-specific DNA in a population of a different species or following contact (e.g., growth) with cells of a different species.
摘要翻译： 本发明涉及用于鉴定药物产品中物种特异性材料的方法和组合物。 特别地，本发明涉及用于鉴定不同物种群体或与不同物种的细胞接触（例如生长）之后的物种特异性DNA的方法。
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公开(公告)号：US20100330046A1
公开(公告)日：2010-12-30
申请号：US12784699
申请日：2010-05-21
CPC分类号： A61K38/208 , A61K35/12 , A61K48/00 , C07K14/5434 , C12N5/0629 , C12N2501/23 , C12N2510/00
摘要： The present invention relates generally to compositions for treating patients that have skin cancer or have recently had skin cancers removed. More specifically, the present invention provides human skin substitutes engineered to express exogenous IL-12 and compositions and methods for making human skin substitutes engineered to express exogenous IL-12. In addition, the present invention provides methods for treatment of sites on a patient where skin cancers have been removed with human skin substitutes engineered to express exogenous IL-12.
摘要翻译： 本发明一般涉及用于治疗患有皮肤癌或最近除去皮肤癌的患者的组合物。 更具体地，本发明提供了工程化以表达外源IL-12的人皮肤代用品，以及用于制备人工皮肤代用品以表达外源性IL-12的组合物和方法。 此外，本发明提供了治疗患者皮肤癌已被去除的部位的方法，人类皮肤替代物被工程化以表达外源IL-12。
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公开(公告)号：US06884595B2
公开(公告)日：2005-04-26
申请号：US10299938
申请日：2002-11-19
IPC分类号： A61P35/00 , A61P43/00 , C12N1/00 , C12N5/071 , C12N5/10 , C12N15/09 , C12Q1/02 , G01N33/15 , G01N33/50 , G01N33/567 , C12N5/08
CPC分类号： C12N5/0629 , C12N5/0698 , C12N2502/094 , C12N2502/1323 , C12N2502/30 , C12N2503/02 , C12N2503/06 , C12N2510/00 , C12N2510/04 , G01N33/5014 , G01N2500/10
摘要： A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. The method comprises the steps of obtaining a human stratified squamous epithelial cell culture, wherein the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, wherein the culture forms a reconstituted epidermis. One then treats the epidermis with a test tumor cell modulation agent and evaluates the growth of the malignant cells within the epidermis.
摘要翻译： 公开了一种自发永生化的人角质形成细胞系。 在优选的实施方案中，该细胞系是ATCC 12191.在本发明的另一个实施方案中，公开了测定肿瘤细胞调节剂的作用的方法。 该方法包括获得人分层鳞状上皮细胞培养物的步骤，其中培养物包含人恶性鳞状上皮细胞和自发永生化人角质形成细胞，其中培养物形成重构表皮。 然后用一种测试的肿瘤细胞调节剂治疗表皮，并评估表皮内恶性细胞的生长。
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公开(公告)号：US09163076B2
公开(公告)日：2015-10-20
申请号：US11365357
申请日：2006-03-01
CPC分类号： C07K14/8146 , A01K2267/035 , A61K35/12 , A61K48/00 , C12N5/0629 , C12N2501/70 , C12N2510/00
摘要： The present invention relates generally to compositions for wound healing. More specifically, the present invention provides human skin equivalents engineered to express exogenous proteinase inhibitor polypeptides (e.g., TIMP-1 polypeptides) and compositions and methods for making human skin equivalents engineered to express exogenous polypeptides. In addition, the present invention provides methods for treatment of wounds with human skin equivalents engineered to express exogenous polypeptides.
摘要翻译： 本发明一般涉及用于伤口愈合的组合物。 更具体地，本发明提供了工程改造以表达外源蛋白酶抑制剂多肽（例如TIMP-1多肽）的人皮肤当量，以及用于制备人皮肤当量的工程化以表达外源多肽的组合物和方法。 此外，本发明提供了用于表达外源多肽的用于治疗具有人皮肤当量的伤口的伤口的方法。
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