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公开(公告)号：US20100261652A1
公开(公告)日：2010-10-14
申请号：US12749507
申请日：2010-03-29
CPC分类号： A61L24/108 , A61F9/007 , A61L2430/16
摘要： There is provided in one embodiment of the disclosure a tissue adhesive composition comprising an engineered protein having repeated blocks of an elastin domain and at least one cell-binding domain and further comprising a polymer crosslinker. When the engineered protein and the polymer crosslinker are introduced onto a tissue, the engineered protein and the polymer crosslinker initiate an in situ crosslinking reaction to form an adhesive bond that is mechanically strong, transparent, biocompatible, and stimulates regrowth of one or more tissue layers over the adhesive bond. In another embodiment of the disclosure there is provided a molded corneal onlay and method of making the same.
摘要翻译： 在本公开的一个实施方案中提供了组织粘合剂组合物，其包含具有弹性蛋白结构域和至少一个细胞结合结构域的重复嵌段的工程改造的蛋白质，并且还包含聚合物交联剂。 当将工程蛋白质和聚合物交联剂引入到组织上时，工程改造的蛋白质和聚合物交联剂引发原位交联反应以形成机械强度，透明度，生物相容性和刺激一个或多个组织层的再生长的粘合剂粘合 超过粘合剂粘合。 在本公开的另一个实施方案中，提供了一种模制的角膜嵌体及其制备方法。
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公开(公告)号：US20090221790A1
公开(公告)日：2009-09-03
申请号：US12247119
申请日：2008-10-07
申请人： David A. Tirrell , Yi Tang
发明人： David A. Tirrell , Yi Tang
摘要： The present invention provides a method for producing modified stable polypeptides introducing at least one non-natural amino acid into the hydrophobic region of the polypeptide. The thermal and chemical stability of such polypeptides is improved compared to those properties of its corresponding wild type proteins.The invention further provides purified leucine zipper and coiled-coil proteins in which the leucine residues have been replaced with 5,5,5-trifluoroleucines, and the modified proteins so produced demonstrate increased thermal and chemical stability compared to their corresponding wild-type natural proteins.
摘要翻译： 本发明提供了一种制备将至少一个非天然氨基酸引入多肽疏水区的修饰稳定多肽的方法。 与其相应的野生型蛋白质的那些性质相比，这些多肽的热和化学稳定性得到改善。 本发明还提供纯化的亮氨酸拉链和卷曲螺旋蛋白，其中亮氨酸残基已经被5,5,5-三氟色氨酸替代，并且与其相应的野生型天然蛋白相比，所产生的修饰的蛋白质表现出增加的热和化学稳定性 。
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公开(公告)号：US07449443B2
公开(公告)日：2008-11-11
申请号：US10851564
申请日：2004-05-21
申请人： David A. Tirrell , Yi Tang
发明人： David A. Tirrell , Yi Tang
摘要： The present invention provides a method for producing modified stable polypeptides introducing at least one non-natural amino acid into the hydrophobic region of the polypeptide. The thermal and chemical stability of such polypeptides is improved compared to those properties of its corresponding wild type proteins.The invention further provides purified leucine zipper and coiled-coil proteins in which the leucine residues have been replaced with 5,5,5-trifluoroleucines, and the modified proteins so produced demonstrate increased thermal and chemical stability compared to their corresponding wild-type natural proteins.
摘要翻译： 本发明提供了一种制备将至少一个非天然氨基酸引入多肽疏水区的修饰稳定多肽的方法。 与其相应的野生型蛋白质的那些性质相比，这些多肽的热和化学稳定性得到改善。 本发明还提供纯化的亮氨酸拉链和卷曲螺旋蛋白，其中亮氨酸残基已经被5,5,5-三氟色氨酸替代，并且与其相应的野生型天然蛋白相比，所产生的修饰的蛋白质表现出增加的热和化学稳定性 。
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4.发明申请OVEREXPRESSION OF AMINOACYL-tRNA SYNTHETASES FOR EFFICIENT PRODUCTION OF ENGINEERED PROTEINS CONTAINING AMINO ACID ANALOGUES 审中-公开用于有效生产含有氨基酸类似物的工程化蛋白质的氨基酸-TRNA合成酶的超表达公开(公告)号：US20080160609A1
公开(公告)日：2008-07-03
申请号：US11743538
申请日：2007-05-02
摘要： Methods for producing modified polypeptides containing amino acid analogues are disclosed. The invention further provides purified dihydrofolate reductase polypeptides, produced by the methods of the invention, in which the methionine residues have been replaced with homoallylglycine, homoproparglycine, norvaline, norleucine, cis-crotylglycine, trans-crotylglycine, 2-aminoheptanoic acid, 2-butynylglycine and allylglycine.
摘要翻译： 公开了生产含有氨基酸类似物的修饰多肽的方法。 本发明进一步提供了通过本发明方法制备的纯化的二氢叶酸还原酶多肽，其中甲硫氨酸残基已经被均一甘氨酸，高丙氨酸，正缬氨酸，正亮氨酸，顺式 - 巴豆基甘氨酸，反 - 巴豆基甘氨酸，2-氨基庚酸，2-丁炔基甘氨酸 和烯丙基甘氨酸。
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公开(公告)号：US07229634B2
公开(公告)日：2007-06-12
申请号：US11040130
申请日：2005-01-21
CPC分类号： C07K14/78 , A01K2217/05 , A01K2227/105 , A61F9/007 , A61L27/227 , A61L27/34 , A61L2430/16 , C07K2319/20 , C07K2319/21 , G02C7/02 , C08L89/00
摘要： The present invention provides engineered proteins and biomedical products made from the engineered proteins. The biomedical products include lenses useful for ophthalmic purposes.
摘要翻译： 本发明提供由工程蛋白质制成的工程化蛋白质和生物医学产品。 生物医学产品包括可用于眼科用途的镜片。
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6.发明授权Rapid, quantitative method for the mass spectrometric analysis of nucleic acids for gene expression and genotyping 失效用于基因表达和基因分型的核酸质谱分析的快速，定量方法公开(公告)号：US06811977B2
公开(公告)日：2004-11-02
申请号：US09918687
申请日：2001-07-27
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C12Q1/6816 , C12Q1/6823 , C12Q1/6827 , C12Q1/6872 , C40B40/00 , Y10T436/143333 , Y10T436/24 , C12Q2565/627 , C12Q2563/167 , C12Q2561/125 , C12Q2531/113 , C12Q2523/319 , C12Q2563/107 , C12Q2523/107
摘要： The invention provides methods of identifying one or more nucleic acids in a sample. The nucleic acids, for example, expressed genes in a cell, can be identified by contacting the nucleic acids with oligonucleotides having detector tags, and selector tags to form tagged oligonucleotides. Each nucleic acid can be uniquely identified by mass-spectrophotometric analysis of the detector tag.
摘要翻译： 本发明提供鉴定样品中一种或多种核酸的方法。 可以通过使核酸与具有检测标签的寡核苷酸和选择标签接触形成标记的寡核苷酸来鉴定核酸，例如细胞中表达的基因。 每个核酸可以通过检测器标签的质谱分析来唯一地鉴定。
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7.发明申请METHODS AND SYSTEMS ASSOCIATED WITH DETECTION OF FATTY ACID ELONGATION IN A CELL 审中-公开与细胞中脂肪酸延伸相关的方法和系统公开(公告)号：US20120231470A1
公开(公告)日：2012-09-13
申请号：US13414670
申请日：2012-03-07
申请人： Janek SZYCHOWSKI , David A. TIRRELL , Sungjin Park
发明人： Janek SZYCHOWSKI , David A. TIRRELL , Sungjin Park
CPC分类号： G01N33/502 , G01N2800/7085
摘要： Methods and systems to identify compounds capable of altering a fatty acid elongation pathway and for identifying conditions under which fatty acids elongation can occur in a cell are described. The methods and systems comprise labeled fatty acid precursors and cells capable of elongating fatty acids. Methods for providing suitable components of an assay for identifying compounds capable of altering a fatty acid elongation pathway are described.
摘要翻译： 描述了鉴定化合物的方法和系统，所述化合物能够改变脂肪酸延长途径并鉴定可在细胞中发生脂肪酸伸长的条件。 方法和系统包括标记的脂肪酸前体和能够延长脂肪酸的细胞。 描述了提供用于鉴定能够改变脂肪酸延长途径的化合物的测定的合适组分的方法。
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公开(公告)号：US08236344B2
公开(公告)日：2012-08-07
申请号：US11757990
申请日：2007-06-04
CPC分类号： C07K14/78 , A01K2217/05 , A01K2227/105 , A61F9/007 , A61L27/227 , A61L27/34 , A61L2430/16 , C07K2319/20 , C07K2319/21 , G02C7/02 , C08L89/00
摘要： The present invention provides engineered proteins and biomedical products made from the engineered proteins. The biomedical products include lenses useful for ophthalmic purposes.
摘要翻译： 本发明提供由工程蛋白质制成的工程化蛋白质和生物医学产品。 生物医学产品包括可用于眼科用途的镜片。
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公开(公告)号：US20090221448A1
公开(公告)日：2009-09-03
申请号：US12210873
申请日：2008-09-15
申请人： David A. Tirrell
发明人： David A. Tirrell
IPC分类号： C40B40/10
CPC分类号： C12N15/62
摘要： The present invention provides a microarray having one or more fusion proteins non-covalently attached to a solid support. Non-covalent attachment is achieved by designing a fusion protein having a polyanionic domain attached to a subject protein, and attaching the fusion protein to a solid support having a polycationic coating.
摘要翻译： 本发明提供了具有一种或多种非共价连接于固体支持物的融合蛋白的微阵列。 通过设计具有连接到受试蛋白的聚阴离子结构域的融合蛋白，并将融合蛋白附着到具有聚阳离子涂层的固体支持物上来实现非共价连接。
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公开(公告)号：US07462463B1
公开(公告)日：2008-12-09
申请号：US10015956
申请日：2001-12-10
申请人： David A. Tirrell
发明人： David A. Tirrell
IPC分类号： G01N33/35
CPC分类号： C12N15/62
摘要： The present invention provides a microarray having one or more fusion proteins non-covalently attached to a solid support. Non-covalent attachment is achieved by designing a fusion protein having a polyanionic domain attached to a subject protein, and attaching the fusion protein to a solid support having a polycationic coating.
摘要翻译： 本发明提供了具有一种或多种非共价连接于固体支持物的融合蛋白的微阵列。 通过设计具有连接到受试蛋白的聚阴离子结构域的融合蛋白，并将融合蛋白附着到具有聚阳离子涂层的固体支持物上来实现非共价连接。
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