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    NUCLEIC ACIDS AND PROTEINS AND METHODS FOR MAKING AND USING THEM
    1.
    发明申请
    NUCLEIC ACIDS AND PROTEINS AND METHODS FOR MAKING AND USING THEM 审中-公开
    核酸和蛋白质及其制备和使用方法

    公开(公告)号：US20110296543A1

    公开(公告)日：2011-12-01

    申请号：US12324436

    申请日：2008-11-26

    申请人： Hwai Chang ,  Eric J. Mathur ,  Michelle Cayouette ,  Dan E. Robertson ,  Philip Hugenholtz ,  Falk Warnecke ,  Jared R. Leadbetter ,  Natalia Ivanova ,  Peter Luginbuhl ,  Don Hutchison

    发明人： Hwai Chang ,  Eric J. Mathur ,  Michelle Cayouette ,  Dan E. Robertson ,  Philip Hugenholtz ,  Falk Warnecke ,  Jared R. Leadbetter ,  Natalia Ivanova ,  Peter Luginbuhl ,  Don Hutchison

    IPC分类号： A01K67/00

    CPC分类号： C12Y302/01015 ,  C12N9/2437 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01039 ,  C12Y302/01067 ,  C12Y302/01082

    摘要： The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs. Polypeptides of the invention can be used in food processing, brewing, bath additives, alcohol production, peptide synthesis, enantioselectivity, hide preparation in the leather industry, waste management and animal degradation, silver recovery in the photographic industry, medical treatment, silk degumming, biofilm degradation, biomass conversion to a biofuel (e.g., a bioethanol, biomethanol, biopropanol or biobutanol, a biodiesel, etc.), biodefense, antimicrobial agents and disinfectants, personal care and cosmetics, biotech reagents, in corn wet milling and pharmaceuticals such as digestive aids and anti-inflammatory (anti-phlogistic) agents.

    摘要翻译： 本发明提供了多肽，包括酶，结构蛋白和结合蛋白，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 本发明的多肽，包括酶和抗体以及核酸可用于工业，实验，食品和饲料加工，营养和药物应用中，例如用于食品和饲料添加剂，着色剂，中性药物，化妆品和药物需求。 本发明的多肽可用于食品加工，酿造，浴添加剂，酒精生产，肽合成，对映选择性，皮革行业中的皮革制备，废物管理和动物降解，摄影工业中的银回收，医疗，丝绸脱胶， 生物膜降解，生物燃料转化为生物燃料（例如生物乙醇，生物甲醇，生物丙醇或生物丁醇，生物柴油等），生物防治剂，抗微生物剂和消毒剂，个人护理和化妆品，生物技术试剂，玉米湿法研磨和药物如 消化助剂和抗炎（抗炎）药剂。

    Production and use of normalized DNA libraries
    7.
    发明授权
    Production and use of normalized DNA libraries 有权
    生产和使用标准化DNA文库

    公开(公告)号：US06444426B1

    公开(公告)日：2002-09-03

    申请号：US09437905

    申请日：1999-11-09

    申请人： Jay M. Short ,  Eric J. Mathur

    发明人： Jay M. Short ,  Eric J. Mathur

    IPC分类号： C12Q170

    CPC分类号： C12N15/1093 ,  C12N15/1096 ,  C12Q1/6811

    摘要： Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (c) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample. Also disclosed is a normalized genomic DNA library formed from an environmental sample by the process.

    摘要翻译： 公开了一种通过（a）从环境样品中分离基因组DNA群体从环境样品形成归一化基因组DNA文库的方法; （b）至少一个（i）扩增如此分离的DNA群体的拷贝数，和（ii）回收具有所需特征的分离的基因组DNA的一部分; 和（c）使基因组DNA群体内的各种DNA的表达正常化，从而形成来自环境样品的基因组DNA的归一化文库。 还公开了通过该方法由环境样品形成的归一化的基因组DNA文库。

    Purified thermostable pyrococcus furiosus DNA ligase
    8.
    发明授权
    Purified thermostable pyrococcus furiosus DNA ligase 失效
    纯化热稳定热激杆菌激酶2 DNA连接酶

    公开(公告)号：US06280998B1

    公开(公告)日：2001-08-28

    申请号：US08916232

    申请日：1997-08-22

    申请人： Eric J. Mathur ,  Edward J. Marsh ,  Warren E. Schoettlin

    发明人： Eric J. Mathur ,  Edward J. Marsh ,  Warren E. Schoettlin

    IPC分类号： C12N1552

    CPC分类号： C12N9/93 ,  Y10S435/822

    摘要： Purified thermostable DNA ligase is described that catalyzes template-dependent ligation at temperatures of about 30° C. to about 80° C., and which substantially retains its catalytic ability when subjected to temperatures of from about 85° C. to about 100° C. The thermostable DNA ligase has an estimated molecular weight of 50,000 to 70,000 daltons. A preferred thermostable DNA ligase is described that was isolated from the archaebacteria Pyrococcus furiosus. Also described are plasmid vectors for producing recombinant thermostable DNA ligase.

    摘要翻译： 描述了纯化的热稳定DNA连接酶，其在约30℃至约80℃的温度下催化模板依赖性连接，并且当经受约85℃至约100℃的温度时，其基本上保持其催化能力 热稳定DNA连接酶的估计分子量为50,000至70,000道尔顿。 描述了从古细菌火球菌（Pyrococcus furiosus）分离的优选的热稳定DNA连接酶。 还描述了用于产生重组热稳定DNA连接酶的质粒载体。