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    Methods of detecting amplified product
    3.
    发明授权
    Methods of detecting amplified product 有权
    检测扩增产物的方法

    公开(公告)号：US08067159B2

    公开(公告)日：2011-11-29

    申请号：US11837600

    申请日：2007-08-13

    申请人： James F. Brown ,  Jonathan E. Silver

    发明人： James F. Brown ,  Jonathan E. Silver

    IPC分类号： G01N33/543

    CPC分类号： C12Q1/6806 ,  B01J2219/00317 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01L3/5027 ,  B01L3/5085 ,  B01L3/50851 ,  B01L3/5088 ,  B01L2200/06 ,  B01L2200/0642 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2400/0409 ,  C07H21/00 ,  C12Q1/6844 ,  C12Q1/686 ,  C40B60/14 ,  Y10S436/805 ,  Y10S436/809 ,  Y10T436/2575

    摘要： A microfluidic device comprising a first surface portion and a first sample retaining element, which have differing affinities to a fluid, and a method comprising supplying a sample to such a device. In some embodiments, the differing affinity is a result of plasma, ion embedding, surface charging, chemical, optical, electronic and/or electromagnetic treatment. Also, a microfluidic device comprising at least one microcapillary device having a sample retaining element, at least one surface of which exhibits hydrophobicity, hydrophilicity, electromagnetic force exertion and electrostatic force exertion. Also, a microfluidic device comprising a first element having a hydrophilic pattern comprising at least a first sample retaining element. Also, a method comprising supplying a sample to a channel between a first element and a second element, and inducing in the first element at least one hydrophilic pattern by electrets or by internal or external electrodes to provide a charged surface.

    摘要翻译： 一种微流体装置，包括与流体具有不同亲和性的第一表面部分和第一样品保持元件，以及包括将样品供应到这种装置的方法。 在一些实施方案中，不同的亲合力是等离子体，离子嵌入，表面充电，化学，光学，电子和/或电磁处理的结果。 此外，微流体装置包括至少一个具有样品保持元件的微毛细管装置，其至少一个表面具有疏水性，亲水性，电磁力消耗和静电力的作用。 此外，微流体装置包括具有至少第一样品保持元件的亲水图案的第一元件。 另外，一种方法包括将样品提供给第一元件和第二元件之间的通道，并且通过驻极体或内部或外部电极在第一元件中诱发至少一种亲水图案以提供带电表面。

    Techniques for producing site-directed mutagenesis of cloned DNA
    5.
    发明授权
    Techniques for producing site-directed mutagenesis of cloned DNA 失效
    用于产生克隆DNA定点诱变的技术

    公开(公告)号：US5284760A

    公开(公告)日：1994-02-08

    申请号：US764085

    申请日：1991-09-23

    申请人： Stephen M. Feinstone ,  Czeslaw Wychowski ,  Jonathan E. Silver ,  Suzanne U. Emerson

    发明人： Stephen M. Feinstone ,  Czeslaw Wychowski ,  Jonathan E. Silver ,  Suzanne U. Emerson

    IPC分类号： C07K14/10 ,  C07K14/105 ,  C12Q1/68 ,  C12N15/10

    CPC分类号： C12Q1/6858 ,  C07K14/005 ,  C12Q1/6853 ,  C07K2319/00 ,  C12N2770/32434 ,  C12N2770/32622

    摘要： A method is described whereby new cDNA or RNA sequences can be introduced into or substituted for cDNA in any chosen position without specific sequence requirements using the polymerase chain reaction (PCR). The method entails the use of primers which are complementary to the 3' and 5' ends of the desired sequence to be inserted as well as to the 3' and 5' ends of the chosen site of insertion in the acceptor molecule. The desired sequence is amplified by PCR such that single stranded fragments are produced. The single stranded fragment of the desired sequence is then annealed to a single stranded acceptor molecule at the site of insertion and extended to produce a double stranded molecule. The double stranded molecule is then separated into two strands which are identical except that one of the strands contains the desired sequence inserted at the chosen site. A second double stranded molecule is then generated.

    摘要翻译： 描述了一种方法，其中可以使用聚合酶链式反应（PCR）将新的cDNA或RNA序列引入任何选定位置或用任何特定序列要求代替cDNA。 该方法需要使用与待插入的所需序列的3'和5'末端互补的引物以及在受体分子中所选插入位点的3'和5'末端。 通过PCR扩增所需的序列，从而产生单链片段。 然后将所需序列的单链片段在插入位点退火至单链受体分子并延伸以产生双链分子。 然后将双链分子分成两条相同的链，除了一条链含有插入所选位点的所需序列外。 然后产生第二双链分子。