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14 条记录 (耗时 0.024 秒)

    Massively parallel signature sequencing by ligation of encoded adaptors
    2.
    再颁专利
    Massively parallel signature sequencing by ligation of encoded adaptors 有权
    通过编码适配器的连接进行大规模并行签名排序

    公开(公告)号：USRE43097E1

    公开(公告)日：2012-01-10

    申请号：US12782008

    申请日：2010-05-18

    申请人： Glenn Albrecht ,  Sydney Brenner ,  Robert B. DuBridge ,  David H. Lloyd ,  Michael C. Pallas

    发明人： Glenn Albrecht ,  Sydney Brenner ,  Robert B. DuBridge ,  David H. Lloyd ,  Michael C. Pallas

    IPC分类号： C12Q1/68 ,  C07H21/02

    CPC分类号： C12Q1/6869 ,  C12Q1/6837 ,  C12Q2525/191 ,  C12Q2563/149 ,  C12Q2563/179

    摘要： The invention provides a method of nucleic acid sequence analysis based on the ligation of one or more sets of encoded adaptors to the terminus of a target polynucleotide. Encoded adaptors whose protruding strands form perfectly matched duplexes with the complementary protruding strands of the target polynucleotide are ligated, and the identity of the nucleotides in the protruding strands is determined by an oligonucleotide tag carried by the encoded adaptor. Such determination, or “decoding” is carried out by specifically hybridizing a labeled tag complement to its corresponding tag on the ligated adaptor.

    摘要翻译： 本发明提供了基于将一组或多组编码衔接子连接到靶多核苷酸末端的核酸序列分析方法。 将其突出的链与靶多核苷酸的互补突出链形成完全匹配的双链体的编码适配子连接，突出的链中的核苷酸的身份由编码的适配器携带的寡核苷酸标签确定。 这样的确定或“解码”是通过将标记的标签补体与连接的适配器上的相应标签进行特异性杂交来进行的。

    Methods, software, and apparatus for focusing an optical system using computer image analysis
    3.
    发明授权
    Methods, software, and apparatus for focusing an optical system using computer image analysis 失效
    使用计算机图像分析聚焦光学系统的方法，软件和装置

    公开(公告)号：US07394943B2

    公开(公告)日：2008-07-01

    申请号：US10881868

    申请日：2004-06-30

    申请人： Patrick D. Kinney ,  Howard G. King ,  Michael C. Pallas ,  Mark Naley

    发明人： Patrick D. Kinney ,  Howard G. King ,  Michael C. Pallas ,  Mark Naley

    IPC分类号： G06K9/40

    CPC分类号： G02B21/244 ,  G02B7/38

    摘要： Methods, software, and apparatus for focusing an image in biological instrument are disclosed. Focusing elements are moved to various focus positions within a focus element travel range, and sample images are captured at the various focus positions. The sample images are resolved into subregions and an optimal focus position is determined based on the image intensity statistical dispersions within the identified subregions.

    摘要翻译： 公开了用于将图像聚焦在生物仪器中的方法，软件和装置。 将聚焦元件移动到聚焦元件行进范围内的各个焦点位置，并且在各个焦点位置捕获采样图像。 样本图像被分解成次区域，并且基于所识别的子区域内的图像强度统计分散来确定最佳焦点位置。

    System and apparatus for sequential processing of analytes
    5.
    发明授权
    System and apparatus for sequential processing of analytes 有权
    用于顺序处理分析物的系统和设备

    公开(公告)号：US06969488B2

    公开(公告)日：2005-11-29

    申请号：US09908131

    申请日：2001-07-17

    申请人： John Bridgham ,  Kevin Corcoran ,  George Golda ,  Sydney Brenner ,  Michael C. Pallas

    发明人： John Bridgham ,  Kevin Corcoran ,  George Golda ,  Sydney Brenner ,  Michael C. Pallas

    IPC分类号： B01L3/00 ,  G01N15/14 ,  G01N21/25 ,  G01N33/48

    CPC分类号： G01N21/253 ,  B01J2219/00274 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0403 ,  B01L2400/0475 ,  B01L2400/0622 ,  B01L2400/0644 ,  G01N2015/144 ,  G01N2015/1452 ,  G01N2035/00158 ,  Y10S435/81 ,  Y10T436/13 ,  Y10T436/143333

    摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.

    摘要翻译： 提供了一种用于同时分析锚定到微粒的多个分析物的装置和系统。 每个具有单一类型分析物的均匀群体的微粒被布置为在流动室内部基本上固定的平面阵列，其中分析过程的步骤通过将一系列处理试剂通过流体系统递送到微粒进行， 微处理器控制。 响应于这样的处理步骤，在每个微粒的表面产生光信号，其特征在于微粒携带的分析物与递送的处理试剂之间的相互作用。 通过收集和记录由平面阵列中的所有微粒产生的光学信号的图像来同时分析多个分析物。 本发明的一个关键特征是在分析过程中由平面阵列中的每个微粒产生的光信号序列的相关性。

    Flat-Field Imaging System and Methods of Use
    6.
    发明申请
    Flat-Field Imaging System and Methods of Use 审中-公开
    平场成像系统和使用方法

    公开(公告)号：US20140203169A1

    公开(公告)日：2014-07-24

    申请号：US14127152

    申请日：2012-06-13

    申请人： Mark Andersen ,  Michael C. Pallas ,  Haopeng Wang

    发明人： Mark Andersen ,  Michael C. Pallas ,  Haopeng Wang

    IPC分类号： G01N15/04

    CPC分类号： G01N15/04 ,  C12Q1/6816 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/607 ,  C12Q2565/629

    摘要： A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.

    摘要翻译： 提供了一种对齐多个目标的方法。 该方法包括生成多个目标。 第三阶段包括多个目标。 该方法还包括组合体积中的第一阶段，第二阶段和第三阶段。 第一相，第二相和第三相基本上是不混溶的，第三相与第一相和第二相流体连通，第一相，第二相和第三相可操作为 在体积中的第一相和第二相之间的第三相的配置。

    System and apparatus for sequential processing of analytes
    10.
    发明授权
    System and apparatus for sequential processing of analytes 有权
    用于顺序处理分析物的系统和设备

    公开(公告)号：US07282370B2

    公开(公告)日：2007-10-16

    申请号：US11207443

    申请日：2005-08-18

    申请人： John Bridgham ,  Kevin Corcoran ,  George Golda ,  Michael C. Pallas ,  Sydney Brenner

    发明人： John Bridgham ,  Kevin Corcoran ,  George Golda ,  Michael C. Pallas ,  Sydney Brenner

    IPC分类号： G01N21/76 ,  G01N21/00 ,  G01N37/00

    CPC分类号： G01N21/253 ,  B01J2219/00274 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0403 ,  B01L2400/0475 ,  B01L2400/0622 ,  B01L2400/0644 ,  G01N2015/144 ,  G01N2015/1452 ,  G01N2035/00158 ,  Y10S435/81 ,  Y10T436/13 ,  Y10T436/143333

    摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.

    摘要翻译： 提供了一种用于同时分析锚定到微粒的多个分析物的装置和系统。 每个具有单一类型分析物的均匀群体的微粒被布置为在流动室内部基本上固定的平面阵列，其中分析过程的步骤通过将一系列处理试剂通过流体系统递送到微粒进行， 微处理器控制。 响应于这样的处理步骤，在每个微粒的表面产生光信号，其特征在于微粒携带的分析物与递送的处理试剂之间的相互作用。 通过收集和记录由平面阵列中的所有微粒产生的光学信号的图像来同时分析多个分析物。 本发明的一个关键特征是在分析过程中由平面阵列中的每个微粒产生的光信号序列的相关性。