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    Method of producing a fibrin monomer using a biotinylated enzyme and immobilized avidin
    4.
    发明授权
    Method of producing a fibrin monomer using a biotinylated enzyme and immobilized avidin 失效
    使用生物素化酶和固定化抗生物素蛋白生产纤维蛋白单体的方法

    公开(公告)号：US6046024A

    公开(公告)日：2000-04-04

    申请号：US904369

    申请日：1997-08-01

    申请人： Steven James Burton ,  James C. Pearson ,  Peter A. D. Edwardson

    发明人： Steven James Burton ,  James C. Pearson ,  Peter A. D. Edwardson

    IPC分类号： C12N11/10 ,  A61L2/00 ,  A61L24/10 ,  C07K1/22 ,  C07K14/36 ,  C07K14/465 ,  C07K14/75 ,  C07K17/10 ,  C12N11/16 ,  C12P21/06 ,  A61K5/00 ,  C07K17/00 ,  C12N11/02 ,  G01N33/53

    CPC分类号： C07K14/36 ,  A61L2/0035 ,  A61L24/106 ,  C07K1/22 ,  C07K14/465 ,  C07K14/75 ,  C07K17/10 ,  C12P21/06 ,  Y10S435/966 ,  Y10S435/967 ,  Y10S435/972 ,  Y10S530/81 ,  Y10S530/812 ,  Y10S530/813

    摘要： Compositions and methods for avidin immobilized on an inert support material, e.g. agarose, are disclosed. The compositions have high activity levels of avidin and may further include a bulking agent, e.g., maltose, and a protectant to maintain the stability and integrity of the avidin agarose during lyophilization and terminal sterilization processes. These compositions have applicability in any instance where avidin agarose and/or the avidin/biotin technology are useful. In particular, the present compositions are useful in an enzyme capture system to prepare fibrin monomer useful for fibrin sealants. The fibrin is prepared by subjecting a fibrinogen-containing composition to a biotinylated enzyme to convert the fibrinogen in the composition to a corresponding fibrin monomer. A fibrin monomer/biotinylated enzyme mixture is formed. A material comprising an avidin/inert support composition having 1000 biotin binding units of activity per gram of the composition, a bulking agent and a protectant, is then introduced into the enzyme mixture to form a complex of the material and the biotinylated enzyme. The complex and enzyme are then removed therefrom to recover a fibrin monomer. Bulking agents selected from nonionic water soluble compounds are used.

    摘要翻译： 固定在惰性载体材料上的抗生物素蛋白的组合物和方法，例如 琼脂糖，被披露。 所述组合物具有高活性水平的抗生物素蛋白，并且还可以包括填充剂，例如麦芽糖，以及在冻干和末端灭菌过程中保持抗生物素蛋白琼脂糖的稳定性和完整性的保护剂。 这些组合物在抗生物素蛋白琼脂糖和/或抗生物素蛋白/生物素技术是有用的任何情况下都具有适用性。 特别地，本发明的组合物可用于酶捕获系统中以制备用于纤维蛋白密封剂的纤维蛋白单体。 通过使含纤维蛋白原的组合物经生物素化的酶将组合物中的纤维蛋白原转化成相应的纤维蛋白单体来制备纤维蛋白。 形成纤维蛋白单体/生物素化的酶混合物。 然后将包含每克组合物具有1000个生物素结合活性单元，一种填充剂和一种保护剂的抗生物素蛋白/惰性支持组合物的材料引入该酶混合物中以形成该材料和生物素化酶的复合物。 然后从中除去复合物和酶以回收纤维蛋白单体。 使用选自非离子水溶性化合物的填充剂。

    Stable biotinylated biomolecule composition
    7.
    发明授权
    Stable biotinylated biomolecule composition 失效
    稳定的生物素化生物分子组成

    公开(公告)号：US06551794B1

    公开(公告)日：2003-04-22

    申请号：US08555602

    申请日：1995-11-09

    申请人： Steven James Burton ,  James C. Pearson ,  Peter A. D. Edwardson ,  Alan Menzies

    发明人： Steven James Burton ,  James C. Pearson ,  Peter A. D. Edwardson ,  Alan Menzies

    IPC分类号： A61K500

    CPC分类号： G01N33/54306 ,  A61L2/0035 ,  C07K14/75

    摘要： Stable compositions containing biotinylated molecules, such as enzymes, are provided. The compositions include a biotinylated biomolecule, a biomolecule protectant, a buffer, a bulking agent selected from one or more water soluble, nonionic polymers and preferably a terminal sterilization protectant. The compositions can be utilized either as aqueous solutions or preferably in dried form, e.g., as a lyophilized, powder cake. They have applicability in any case where avidin/biotin technology is used and are particularly important as compositions containing a thrombin-like enzyme, e.g., for preparation of a fibrin monomer and fibrin monomer-based fibrin sealants.

    摘要翻译： 提供含有生物素化分子如酶的稳定组合物。 组合物包括生物素化的生物分子，生物分子保护剂，缓冲剂，选自一种或多种水溶性非离子聚合物的填充剂，优选末端灭菌保护剂。 组合物可以作为水溶液或优选以干燥形式使用，例如冻干的粉饼。 它们在使用抗生物素蛋白/生物素技术的任何情况下都具有适用性，并且对于含有凝血酶样酶的组合物是特别重要的，例如用于制备纤维蛋白单体和基于纤维蛋白单体的纤维蛋白密封剂。

    Gel, slurry or suspension containing immobilized avidin having high biotin binding activity
    9.
    发明授权
    Gel, slurry or suspension containing immobilized avidin having high biotin binding activity 失效
    含有具有高生物素结合活性的固定化抗生物素蛋白的凝胶，浆液或悬浮液

    公开(公告)号：US5942406A

    公开(公告)日：1999-08-24

    申请号：US904367

    申请日：1997-08-01

    申请人： Steven James Burton ,  James C. Pearson ,  Peter A. D. Edwardson

    发明人： Steven James Burton ,  James C. Pearson ,  Peter A. D. Edwardson

    IPC分类号： C12N11/10 ,  A61L2/00 ,  A61L24/10 ,  C07K1/22 ,  C07K14/36 ,  C07K14/465 ,  C07K14/75 ,  C07K17/10 ,  C12N11/16 ,  C12P21/06 ,  C12N11/02 ,  G01N33/53

    CPC分类号： C07K14/36 ,  A61L2/0035 ,  A61L24/106 ,  C07K1/22 ,  C07K14/465 ,  C07K14/75 ,  C07K17/10 ,  C12P21/06 ,  Y10S435/966 ,  Y10S435/967 ,  Y10S435/972 ,  Y10S530/81 ,  Y10S530/812 ,  Y10S530/813

    摘要： Compositions and methods for avidin immobilized on an inert support material, e.g., agarose, are disclosed. The compositions have high activity levels of avidin and may further include a bulking agent, e.g., maltose, and a protectant to maintain the stability and integrity of the avidin agarose during lyophilization and terminal sterilization processes. A dry composition, and wet compositions such as a gel, slurry or suspension having avidin immobilized on an inert support material are disclosed. The dry composition has at least 1000 biotin binding units of activity, and the wet compositions have at least 50 units of binding activity. These compositions have applicability in any instance where avidin agarose and/or the avidin/biotin technology are useful. In particular, the present compositions are useful in an enzyme capture system to prepare fibrin monomer useful for fibrin sealants.

    摘要翻译： 公开了固定在惰性载体材料例如琼脂糖上的抗生物素蛋白的组合物和方法。 所述组合物具有高活性水平的抗生物素蛋白，并且还可以包括填充剂，例如麦芽糖，以及在冻干和末端灭菌过程中保持抗生物素蛋白琼脂糖的稳定性和完整性的保护剂。 公开了一种干组合物和湿固组合物，例如固定在惰性载体材料上的具有亲和素的凝胶，浆液或悬浮液。 干组合物具有至少1000个生物素结合单元的活性，湿组合物具有至少50单位的结合活性。 这些组合物在抗生物素蛋白琼脂糖和/或抗生物素蛋白/生物素技术是有用的任何情况下都具有适用性。 特别地，本发明的组合物可用于酶捕获系统中以制备用于纤维蛋白密封剂的纤维蛋白单体。