公开(公告)号：US08709830B2

公开(公告)日：2014-04-29

申请号：US13423092

申请日：2012-03-16

Applicant: Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Hye Eun Hoover

Inventor： Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Hye Eun Hoover

IPC: G01N33/532 ,  G01N33/533 ,  C07K1/13 ,  G01N33/58 ,  G01N33/68 ,  C07K17/06

CPC classification number: G01N1/30 ,  C07K1/13 ,  C07K17/06 ,  C09B11/24 ,  C09B23/06 ,  C09B23/083 ,  C09B57/001 ,  C09B57/02 ,  G01N33/53 ,  G01N33/532 ,  G01N33/533 ,  G01N33/582 ,  G01N33/6854

Abstract: The present invention provides methods, compositions, and kits useful in preparing labeled molecules, which are useful in the detection of binding partners.

Abstract translation: 本发明提供了可用于制备标记分子的方法，组合物和试剂盒，其可用于检测结合配偶体。

公开(公告)号：US08569506B2

公开(公告)日：2013-10-29

申请号：US13556643

申请日：2012-07-24

Applicant: Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

Inventor： Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

IPC: C07D215/00

CPC classification number: G01N1/30 ,  A61K41/0057 ,  C07C66/02 ,  C07D209/08 ,  C07D209/12 ,  C07D209/14 ,  C07D209/16 ,  C07D209/18 ,  C07D209/24 ,  C07D209/54 ,  C07D209/60 ,  C07D209/96 ,  C07D401/04 ,  C07D403/06 ,  C07D403/12 ,  C07D403/14 ,  C07D417/06 ,  C07D417/14 ,  C07D471/04 ,  C07D498/04 ,  C07K1/13 ,  C09B23/02 ,  C09B23/06 ,  C09B23/083 ,  C09B23/107 ,  C12Q1/6825 ,  C40B70/00 ,  G01N31/22 ,  G01N33/533 ,  G01N33/535 ,  G01N33/58 ,  G01N33/581 ,  G01N33/582 ,  G01N2001/302 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

公开(公告)号：US08470529B2

公开(公告)日：2013-06-25

申请号：US12851030

申请日：2010-08-05

Applicant: Jason Dallwig ,  David Hagen ,  Ching-Ying Cheung ,  Gerald Thomas ,  Stephen Yue

Inventor： Jason Dallwig ,  David Hagen ,  Ching-Ying Cheung ,  Gerald Thomas ,  Stephen Yue

IPC: A61K31/4709 ,  C07D215/12 ,  C12Q1/68

CPC classification number: C09B23/164 ,  C07D277/64 ,  C07D417/06 ,  C09B23/0008 ,  C09B23/04 ,  C09B23/06 ,  C09B23/107 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/16 ,  G01N33/5308 ,  Y10T436/143333

Abstract: Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.

公开(公告)号：US08436170B2

公开(公告)日：2013-05-07

申请号：US12699778

申请日：2010-02-03

Applicant: Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung

Inventor： Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung

IPC: C07D405/14 ,  C07D405/12 ,  C07D491/22 ,  C07D491/147 ,  C07D311/88

CPC classification number: G01N33/57484 ,  C07D311/88 ,  C07D405/12 ,  C07D405/14 ,  C07D491/147 ,  C07D491/22 ,  C07K1/13 ,  C09B11/24 ,  G01N33/582

Abstract: The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

公开(公告)号：US08252932B2

公开(公告)日：2012-08-28

申请号：US12906304

申请日：2010-10-18

Applicant: Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

Inventor： Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

IPC: C07D215/00

CPC classification number: G01N1/30 ,  A61K41/0057 ,  C07C66/02 ,  C07D209/08 ,  C07D209/12 ,  C07D209/14 ,  C07D209/16 ,  C07D209/18 ,  C07D209/24 ,  C07D209/54 ,  C07D209/60 ,  C07D209/96 ,  C07D401/04 ,  C07D403/06 ,  C07D403/12 ,  C07D403/14 ,  C07D417/06 ,  C07D417/14 ,  C07D471/04 ,  C07D498/04 ,  C07K1/13 ,  C09B23/02 ,  C09B23/06 ,  C09B23/083 ,  C09B23/107 ,  C12Q1/6825 ,  C40B70/00 ,  G01N31/22 ,  G01N33/533 ,  G01N33/535 ,  G01N33/58 ,  G01N33/581 ,  G01N33/582 ,  G01N2001/302 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了结合通过反应性基团或共轭物质在3位被取代的吲哚环部分的化学活性碳菁染料及其用途。 通过该位置的共轭导致光谱性质均匀地优于光谱相似的染料的缀合物，其中附着在不同的位置。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：US07943777B2

公开(公告)日：2011-05-17

申请号：US12573809

申请日：2009-10-05

Applicant: Jolene Bradford ,  Ching-Ying Cheung ,  Shih-Jung Huang ,  Patrick Pinson ,  Stephen Yue

Inventor： Jolene Bradford ,  Ching-Ying Cheung ,  Shih-Jung Huang ,  Patrick Pinson ,  Stephen Yue

IPC: C07D417/00 ,  C07D215/00 ,  C07D251/38

CPC classification number: G01N33/582 ,  C07D215/10 ,  C07D215/227 ,  C07D277/72 ,  C07D277/74 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  C12Q1/6876 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2201/06113 ,  Y10T436/143333

Abstract: Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.

Abstract translation: 公开了对RNA和单链DNA具有对双链DNA的高选择性的化合物。 化学化合物是在照射时变成荧光并与双链DNA相互作用的污渍，但在不存在双链DNA的情况下表现出减少的或没有荧光。 该化合物可用于各种生物学应用中，以定性或定量测定DNA，即使在RNA的存在下。

公开(公告)号：US20110097735A1

公开(公告)日：2011-04-28

申请号：US12607915

申请日：2009-10-28

Applicant: Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung

Inventor： Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung

IPC: G01N33/53 ,  C07K5/10 ,  C07K16/18 ,  C07K1/107 ,  C07C245/24 ,  C07H21/00 ,  C08B37/00 ,  C07D401/12 ,  C07D207/46

CPC classification number: C07C309/47 ,  C07C2603/50 ,  C07D207/46 ,  C07D401/12 ,  C07K1/13 ,  C08H1/00 ,  G01N33/533 ,  G01N33/57484 ,  G01N2333/475

Abstract: The present invention relates to fluorescent pyrene dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

Abstract translation: 本发明一般涉及荧光芘染料。 本发明提供了广泛的荧光染料和含有该荧光染料的试剂盒，其可用于标记各种生物分子，细胞和微生物。 本发明还提供使用荧光染料进行研究，开发鉴定，环境研究，诊断，预后和/或治疗疾病的各种方法。

公开(公告)号：US07927830B2

公开(公告)日：2011-04-19

申请号：US12129671

申请日：2008-05-29

Applicant: Ching-Ying Cheung ,  Stephen Yue ,  Wai-Yee Leung

Inventor： Ching-Ying Cheung ,  Stephen Yue ,  Wai-Yee Leung

IPC: G01N1/30 ,  G01N33/68 ,  G01N33/53

CPC classification number: G01N1/30 ,  A61K41/0057 ,  C07C66/02 ,  C07D209/08 ,  C07D209/12 ,  C07D209/14 ,  C07D209/16 ,  C07D209/18 ,  C07D209/24 ,  C07D209/54 ,  C07D209/60 ,  C07D209/96 ,  C07D401/04 ,  C07D403/06 ,  C07D403/12 ,  C07D403/14 ,  C07D417/06 ,  C07D417/14 ,  C07D471/04 ,  C07D498/04 ,  C07K1/13 ,  C09B23/02 ,  C09B23/06 ,  C09B23/083 ,  C09B23/107 ,  C12Q1/6825 ,  C40B70/00 ,  G01N31/22 ,  G01N33/533 ,  G01N33/535 ,  G01N33/58 ,  G01N33/581 ,  G01N33/582 ,  G01N2001/302 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了结合通过反应性基团或共轭物质在3位被取代的吲哚环部分的化学活性碳菁染料及其用途。 通过该位置的共轭导致光谱性质均匀地优于光谱相似的染料的缀合物，其中附着在不同的位置。 本发明包括具有一个或多个苯并氮的衍生物化合物。

公开(公告)号：US20110082947A1

公开(公告)日：2011-04-07

申请号：US12723615

申请日：2010-03-12

Applicant: Ronald W. Szeto ,  David Chun Ying Cheung ,  Rajkumar Jalan

Inventor： Ronald W. Szeto ,  David Chun Ying Cheung ,  Rajkumar Jalan

IPC: G06F15/16

CPC classification number: H04L67/1036 ,  H04L63/0218 ,  H04L67/1002 ,  H04L67/32

Abstract: Each service in a computer network may have a connection rate limit. The number of new connections per time period may be limited by using a series of rules. In a specific embodiment of the present invention, a counter is increased each time a server is selected to handle a connection request. For each service, connections coming in are tracked. Therefore, the source of connection-request packets need not be examined. Only the destination service is important. This saves significant time in the examination of the incoming requests. Each service may have its own set of rules to best handle the new traffic for its particular situation.

Abstract translation: 计算机网络中的每个服务可能具有连接速率限制。 每个时间段的新连接数可以通过使用一系列规则来限制。 在本发明的具体实施例中，每当选择服务器来处理连接请求时，增加计数器。 对于每个服务，跟踪进入的连接。 因此，不需要检查连接请求数据包的来源。 只有目的地服务很重要。 这样可以节省大量时间来检查传入的请求。 每个服务可能有自己的一套规则，以便为特定情况最好地处理新的流量。

公开(公告)号：US07888136B2

公开(公告)日：2011-02-15

申请号：US12099020

申请日：2008-04-07

Applicant: Stephen Yue ,  Ching-Ying Cheung

Inventor： Stephen Yue ,  Ching-Ying Cheung

IPC: G01N33/53 ,  B01L3/00 ,  C12Q1/68 ,  C07H19/04

CPC classification number: C12Q1/6816 ,  Y10S436/80 ,  C12Q2563/173

Abstract: Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties. The present nucleic acid reporter molecules find utility in forming a nucleic acid-reporter molecule complex and detecting the nucleic acid. In particular, present nucleic acid reporter molecules with a rigid linker and monomer moieties with a monomethine bridge find utility in detecting RNA in the presence of DNA.

Abstract translation: 本发明的实施方案提供了用于检测样品中核酸的方法和核酸报告分子。 核酸报告分子包含两个不对称的花青单体部分，其可以相同或不同，其通过包含至少一个芳族，杂芳族，环状或杂环部分的连接体共价连接，该部分包含3-20个非氢原子，所述非氢原子选自 由O，N，S，P和C组成的组。接头可以是刚性的，相对柔性的或其某种程度的。 不对称花青单体部分包含取代或未取代的苯并唑鎓部分和通过单甲基，三甲基或五甲氧基的次甲基桥连接的取代或未取代的吡啶鎓或喹啉鎓部分。 连接体通过连接到单体部分的吡啶鎓或喹啉鎓部分而形成花青二聚体化合物。 本发明的核酸报告分子可用于形成核酸 - 报道分子复合物并检测核酸。 特别地，具有刚性连接体和具有单甲基桥的单体部分的本发明的核酸报告分子在DNA存在下可用于检测RNA。