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1.发明申请公开(公告)号：US20180008982A1
公开(公告)日：2018-01-11
申请号：US15544761
申请日：2016-01-21
Applicant: Agency for Science, Technology and Research
Inventor： Jackie Y. Ying , Min-Han Tan , Yoke San Daniel Lee , Igor Cima , Yeon Joon Park , Wai Min Phyo
IPC: B01L3/00 , C12Q1/68 , G01N33/574
CPC classification number: B01L3/502761 , B01L2200/027 , B01L2200/0647 , B01L2300/042 , B01L2300/0681 , C12N5/069 , C12Q1/6886 , C12Q2600/156 , G01N33/5064 , G01N33/5094 , G01N33/574 , G01N33/57419 , G01N33/57492
Abstract: A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.
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公开(公告)号：US10463646B2
公开(公告)日：2019-11-05
申请号：US16039098
申请日：2018-07-18
Applicant: Agency for Science, Technology and Research
Inventor： Motoichi Kurisawa , Yongvongsoontorn Nunnarpas , Jackie Y. Ying , Joo Eun Chung , Ki Hyun Bae , Min-Han Tan , Esther Lee
IPC: A61K31/404 , C08G65/331 , C08G65/332 , C08G65/333 , A61K31/353 , A61K47/10 , A61K9/107 , C08L1/02 , C08L3/02 , C08L5/00 , C08L5/02 , C08L5/16 , C08L89/00 , A61K47/60 , A61K31/704 , A61K47/59 , B82Y5/00
Abstract: The present invention relates to micellar nanocomplexes and a method of forming the same. The micellar nanocomplex comprises a micelle and an agent encapsulated within said micelle, where the micelle comprises a polymer-flavonoid conjugate, wherein said polymer is bonded to the B ring of said flavonoid. The micellar nanocomplex may have useful applications as a drug-delivery system.
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公开(公告)号：US11701657B2
公开(公告)日：2023-07-18
申请号：US17077828
申请日：2020-10-22
Applicant: Agency for Science, Technology and Research
Inventor： Jackie Y. Ying , Min-Han Tan , Yoke San Daniel Lee , Igor Cima , Yeon Joon Park , Wai Min Phyo
IPC: B01L3/00 , C12N5/071 , G01N33/50 , G01N33/574 , C12Q1/6886
CPC classification number: B01L3/502761 , C12N5/069 , C12Q1/6886 , G01N33/5064 , G01N33/5094 , G01N33/574 , G01N33/57419 , G01N33/57492 , B01L2200/027 , B01L2200/0647 , B01L2300/042 , B01L2300/0681 , C12Q2600/156
Abstract: A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.
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4.发明授权公开(公告)号：US11084034B2
公开(公告)日：2021-08-10
申请号：US15544761
申请日：2016-01-21
Applicant: Agency for Science, Technology and Research
Inventor： Jackie Y. Ying , Min-Han Tan , Yoke San Daniel Lee , Igor Cima , Yeon Joon Park , Wai Min Phyo
IPC: C12N5/071 , G01N33/50 , G01N33/574 , B01L3/00 , C12Q1/6886
Abstract: A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.
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公开(公告)号：US09901649B2
公开(公告)日：2018-02-27
申请号：US15145020
申请日：2016-05-03
Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION , AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Willy Chin , Yukti Choudhury , Shujun Gao , James L. Hedrick , Xiyu Ke , Min-Han Tan , Jye Yng Teo , Yi Yan Yang
CPC classification number: A61K47/60 , A61K9/0019 , A61K9/1075 , A61K31/704 , A61K47/58 , C08G63/42 , C08G63/78 , C08G64/02 , C08G64/025 , C08G64/183
Abstract: Amphiphilic block copolymers (BCPs) were prepared comprising a poly(ethylene oxide) block and a biodegradable polycarbonate block functionalized with disulfide groups and carboxylic acid groups. The BCPs form self-assembled micellar particles in aqueous solution that can be loaded with hydrophobic drugs for therapeutic drug delivery. The loaded particles have small particle sizes (
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Patent Agency Ranking
6.发明申请公开(公告)号：US20210106997A1
公开(公告)日：2021-04-15
申请号：US17077828
申请日：2020-10-22
Applicant: Agency for Science, Technology and Research
Inventor： Jackie Y. Ying , Min-Han Tan , Yoke San Daniel Lee , Igor Cima , Yeon Joon Park , Wai Min Phyo
IPC: B01L3/00 , C12N5/071 , G01N33/50 , G01N33/574 , C12Q1/6886
Abstract: A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.
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公开(公告)号：US20170319704A1
公开(公告)日：2017-11-09
申请号：US15145020
申请日：2016-05-03
Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION , AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Willy Chin , Yukti Choudhury , Shujun Gao , James L. Hedrick , Xiyu Ke , Min-Han Tan , Jye Yng Teo , Yi Yan Yang
CPC classification number: A61K47/60 , A61K9/0019 , A61K9/1075 , A61K31/704 , A61K47/58 , C08G63/42 , C08G63/78 , C08G64/02 , C08G64/025 , C08G64/183
Abstract: Amphiphilic block copolymers (BCPs) were prepared comprising a poly(ethylene oxide) block and a biodegradable polycarbonate block functionalized with disulfide groups and carboxylic acid groups. The BCPs form self-assembled micellar particles in aqueous solution that can be loaded with hydrophobic drugs for therapeutic drug delivery. The loaded particles have small particle sizes (
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公开(公告)号：US20160230234A1
公开(公告)日：2016-08-11
申请号：US15022917
申请日：2014-09-17
Inventor： Min-Han Tan , Yukti Choudhury , Puay Hoon Tan , Bin Tean Teh
IPC: C12Q1/68
CPC classification number: C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , C12Q2600/158 , C12Q2600/16 , C12Q2600/166
Abstract: A method of making a prognosis as to whether a patient having renal cancer is like to survive in a tumour tissue sample obtained from the patient is provided. The method comprising determining the level of expression for each marker of a panel of markers comprising at least one housekeeping gene selected from the group consisting of ACTB, RPL13A, RPL9, and RPS29 and any combinations thereof and at least one prognostic gene selected from the group consisting of CXCL5, EFNA5, EMCN, G6PC, GFPT2, HIST2H3C, IGFBP1, LAMB3, MMP9, MOCOS, PLG, PRAME, RARRES1, SDPR, SLC6A19, TK1, KDELR3 and TSPAN7 and any combinations thereof, comparing the level of expression of each marker with a predetermined reference level associated with each marker, and determining the differential expression of each marker in the tumour tissue sample based on the expression parameter for each marker to provide a prognosis for renal cancer.
Abstract translation: 提供了一种关于患有肾癌的患者是否希望在从患者获得的肿瘤组织样品中存活的预后的方法。 所述方法包括确定包含至少一种选自ACTB，RPL13A，RPL9和RPS29及其任何组合的至少一个持家基因的标志物组的每个标志物的表达水平和至少一种选自下组的预后基因 由CXCL5，EFNA5，EMCN，G6PC，GFPT2，HIST2H3C，IGFBP1，LAMB3，MMP9，MOCOS，PLG，PRAME，RARRES1，SDPR，SLC6A19，TK1，KDELR3和TSPAN7及其任何组合组成，比较每个标志物的表达水平 具有与每个标记相关联的预定参考水平，并且基于每个标记物的表达参数确定肿瘤组织样品中每个标记物的差异表达以提供肾癌的预后。
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9.发明申请公开(公告)号：US20220001385A1
公开(公告)日：2022-01-06
申请号：US17360014
申请日：2021-06-28
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Jackie Y. Ying , Min-Han Tan , Yoke San Daniel Lee , Igor Cima , Yeon Joon Park , Wai Min Phyo
IPC: B01L3/00 , C12N5/071 , G01N33/50 , G01N33/574 , C12Q1/6886
Abstract: A device and method for retrieving cells of interest, in particular rare cells, were disclosed. Tumor-derived endothelial cell clusters (TECCs) retrieved using the disclosed device and method can be used for the diagnosis and prognosis of cancer.
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公开(公告)号：US11186865B2
公开(公告)日：2021-11-30
申请号：US15541295
申请日：2016-01-21
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Min-Han Tan , Igor Cima
IPC: C12Q1/68 , C12Q1/686 , C12Q1/6806 , C12Q1/6876
Abstract: A method of simultaneously analyzing RNA and DNA in a sample, the method comprising the step (a) contacting the sample with a reverse primer from a first primer pair directed to a target RNA region to effect reverse transcription of RNA into cDNA with a reverse transcriptase; (b) subsequently contacting the sample with (i) a forward primer from the first primer pair directed to a second cDNA region, (ii) a forward and a reverse primers from a second primer pair targeted to a DNA region, and (ii) a DNA polymerase to simultaneously amplify the target cDNA and target DNA region; and (c) analyzing the amplified target cDNA region and/or amplified target DNA region. Also encompassed are uses of the method to analyze gene expression and mutations, kits comprising primers, enzymes, buffers.
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