利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

15 条记录 (耗时 0.028 秒)

    Methods, devices and systems for characterizing proteins
    1.
    发明申请
    Methods, devices and systems for characterizing proteins 审中-公开
    表征蛋白质的方法，装置和系统

    公开(公告)号：US20050027111A1

    公开(公告)日：2005-02-03

    申请号：US10769296

    申请日：2004-01-30

    申请人： Andrea Chow ,  Bahram Fathollahi ,  James Mikkelsen ,  Michael Spaid ,  Adrian Winoto

    发明人： Andrea Chow ,  Bahram Fathollahi ,  James Mikkelsen ,  Michael Spaid ,  Adrian Winoto

    IPC分类号： C07K1/24 ,  G01N27/447 ,  C07K1/10

    CPC分类号： G01N27/44747 ,  C07K1/24 ,  G01N27/44791

    摘要： A method of characterizing a polypeptide, comprising providing a first capillary channel having a separation buffer disposed within, wherein the separation buffer comprises a non-crosslinked polymer solution, a buffering agent, a detergent, and a lipophilic dye. The separation buffer is provided such that, at the time of detection, the detergent concentration in the buffer is not above the critical micelle concentration. The polypeptide is introduced into one end of the capillary channel. An electric field is applied across a length of the capillary channel, which transports polypeptides of different sizes through the polymer solution at different rates. The polypeptide is then detected as it passes a point along the length of the capillary channel.

    摘要翻译： 一种表征多肽的方法，包括提供其中设置有分离缓冲液的第一毛细通道，其中所述分离缓冲液包含非交联聚合物溶液，缓冲剂，洗涤剂和亲脂性染料。 提供分离缓冲液，使得在检测时，缓冲液中的洗涤剂浓度不高于临界胶束浓度。 将多肽引入毛细管通道的一端。 在毛细通道的一段长度上施加电场，毛细通道以不同的速率输送不同尺寸的多肽通过聚合物溶液。 然后当毛细通道沿着毛细通道的长度通过点时，检测该多肽。