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21 条记录 (耗时 0.027 秒)

    Methods, devices and systems for characterizing proteins
    1.
    发明申请
    Methods, devices and systems for characterizing proteins 审中-公开
    表征蛋白质的方法，装置和系统

    公开(公告)号：US20050027111A1

    公开(公告)日：2005-02-03

    申请号：US10769296

    申请日：2004-01-30

    申请人： Andrea Chow ,  Bahram Fathollahi ,  James Mikkelsen ,  Michael Spaid ,  Adrian Winoto

    发明人： Andrea Chow ,  Bahram Fathollahi ,  James Mikkelsen ,  Michael Spaid ,  Adrian Winoto

    IPC分类号： C07K1/24 ,  G01N27/447 ,  C07K1/10

    CPC分类号： G01N27/44747 ,  C07K1/24 ,  G01N27/44791

    摘要： A method of characterizing a polypeptide, comprising providing a first capillary channel having a separation buffer disposed within, wherein the separation buffer comprises a non-crosslinked polymer solution, a buffering agent, a detergent, and a lipophilic dye. The separation buffer is provided such that, at the time of detection, the detergent concentration in the buffer is not above the critical micelle concentration. The polypeptide is introduced into one end of the capillary channel. An electric field is applied across a length of the capillary channel, which transports polypeptides of different sizes through the polymer solution at different rates. The polypeptide is then detected as it passes a point along the length of the capillary channel.

    摘要翻译： 一种表征多肽的方法，包括提供其中设置有分离缓冲液的第一毛细通道，其中所述分离缓冲液包含非交联聚合物溶液，缓冲剂，洗涤剂和亲脂性染料。 提供分离缓冲液，使得在检测时，缓冲液中的洗涤剂浓度不高于临界胶束浓度。 将多肽引入毛细管通道的一端。 在毛细通道的一段长度上施加电场，毛细通道以不同的速率输送不同尺寸的多肽通过聚合物溶液。 然后当毛细通道沿着毛细通道的长度通过点时，检测该多肽。

    METHODS OF DETECTING LOW COPY NUCLEIC ACIDS
    7.
    发明申请
    METHODS OF DETECTING LOW COPY NUCLEIC ACIDS 有权
    检测低拷贝核酸的方法

    公开(公告)号：US20080090244A1

    公开(公告)日：2008-04-17

    申请号：US11873753

    申请日：2007-10-17

    申请人： Michael Knapp ,  Jill Baker ,  Andrea Chow ,  Anne Kopf-Sill ,  Michael Spaid

    发明人： Michael Knapp ,  Jill Baker ,  Andrea Chow ,  Anne Kopf-Sill ,  Michael Spaid

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/1827 ,  B01L2400/0406 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q2531/113 ,  C12Q2565/629 ,  C12Q2527/143 ,  C12Q2547/107 ,  C12Q2537/157

    摘要： Methods are provided for detecting low copy nucleic acids of interest in a sample. In one method, a sample comprising a nucleic acid of interest is aliquotted into a plurality of reaction mixtures, at least two of which are single-copy reaction mixtures. The reaction mixtures are subjected to one or more amplification reactions while flowing through a channel of a microfluidic device. At least one of the reaction mixtures is formulated in an aqueous phase of an emulsion comprising aqueous droplets suspended in an immiscible liquid. The nucleic acid of interest is present as a single copy in at least one aqueous droplet of the aqueous phase prior to performing the amplification reaction(s). Amplification is performed on the reaction mixture when it is formulated in the emulsion. The nucleic acid is continuously flowed during a plurality of steps of the method.

    摘要翻译： 提供了用于检测样品中感兴趣的低拷贝核酸的方法。 在一种方法中，将包含感兴趣的核酸的样品等分成多个反应混合物，其中至少两个是单拷贝反应混合物。 反应混合物在流过微流体装置的通道时进行一次或多次扩增反应。 将至少一种反应混合物配制在包含悬浮在不混溶液体中的水性液滴的乳液的水相中。 感兴趣的核酸在进行扩增反应之前以水相的至少一个水滴形式存在于单拷贝中。 当将其配制在乳液中时，对反应混合物进行扩增。 在该方法的多个步骤期间，核酸连续流动。

    Sequence Determination By Use Of Opposing Forces
    8.
    发明申请
    Sequence Determination By Use Of Opposing Forces 审中-公开
    使用反对力的序列确定

    公开(公告)号：US20110059864A1

    公开(公告)日：2011-03-10

    申请号：US12877103

    申请日：2010-09-07

    申请人： Javier Farinas ,  Andrea Chow ,  John Wallace Parce

    发明人： Javier Farinas ,  Andrea Chow ,  John Wallace Parce

    IPC分类号： C40B30/10 ,  C12M1/34

    CPC分类号： C12Q1/6872 ,  C12Q1/6874

    摘要： The present teachings relate to systems, methods, and the like, for analyzing biological polymers, by use of opposing forces. Among other things, the present teachings can be used to determine sequence information, such as in genetic sequencing and genotyping applications. Various embodiments are described for efficient, high throughput sequencing of nucleic-acid molecules, such as DNA. Various embodiments are described wherein nucleic-acid sequence information is determined without the need or use of extrinsic labels. As well various embodiments of methods, systems, and the like, are described, which can provide long and accurate read lengths for low-cost nucleic acid sequencing.

    摘要翻译： 本教导涉及通过使用相反的力分析生物聚合物的系统，方法等。 除其他之外，本教导可用于确定序列信息，例如在遗传测序和基因分型应用中。 描述了用于核酸分子（例如DNA）的有效，高通量测序的各种实施方案。 描述了其中确定核酸序列信息而不需要或使用外在标记的各种实施方案。 还描述了方法，系统等的各种实施方案，其可以为低成本核酸测序提供长且准确的读取长度。

    High throughput separations based analysis systems and methods
    9.
    发明申请
    High throughput separations based analysis systems and methods 审中-公开
    基于高通量分离的分析系统和方法

    公开(公告)号：US20070119711A1

    公开(公告)日：2007-05-31

    申请号：US11698363

    申请日：2007-01-25

    申请人： Walter Ausserer ,  Luc Bousse ,  Robert Dubrow ,  Steven Sundberg ,  Andrea Chow ,  Benjiamin Wang

    发明人： Walter Ausserer ,  Luc Bousse ,  Robert Dubrow ,  Steven Sundberg ,  Andrea Chow ,  Benjiamin Wang

    IPC分类号： C07K1/26 ,  G01N27/00

    CPC分类号： C07K1/26 ,  G01N27/447

    摘要： Methods and systems for use in separating sample materials into different fractions employing pressure-based fluid flow for simultaneous loading of a sample and a reagent into a sample loading channel of a microfluidic device. The sample is loaded from an external source through an attached external sampling capillary. The reagent, which may be a molecular weight standard, a diluent, a detergent, or a labeling reagent, is loaded from a reservoir integral to the microfluidic device via a reagent introduction channel within the device. The sample and reagent form a mixture in the sample loading channel. A portion of the mixture is electrokinetically injected from the sample loading channel, via an injection channel, into a separation channel, where it is separated electrophoretically.

    摘要翻译： 用于将样品材料分离成不同级分的方法和系统，其使用基于压力的流体流将样品和试剂同时装载到微流体装置的样品加载通道中。 样品通过附带的外部取样毛细管从外部来源加载。 可以是分子量标准的试剂，稀释剂，洗涤剂或标记试剂，通过装置内的试剂引入通道从集成在微流体装置中的储存器装载。 样品和试剂在样品加载通道中形成混合物。 将混合物的一部分从样品加载通道经由注射通道电动注射到分离通道中，其中电泳分离。