-
公开(公告)号:US20110272282A1
公开(公告)日:2011-11-10
申请号:US13128387
申请日:2009-11-10
申请人: Andrea Marziali , David John Broemeling , Joel Pel , Jason Donald Thompson , Jaryn Perkins , Thomas Willis , Herbert Heyneker , Darren Gray , Carolina Tropini
发明人: Andrea Marziali , David John Broemeling , Joel Pel , Jason Donald Thompson , Jaryn Perkins , Thomas Willis , Herbert Heyneker , Darren Gray , Carolina Tropini
CPC分类号: C07K1/24 , B01D57/02 , C12N15/101 , C12Q1/6806 , C12Q2565/125 , C12Q2525/125
摘要: Methods and apparatus for concentrating particles may be applied, for example, to concentrating DNA, RNA, proteins and the like. Proteins may be pre-treated to facilitate concentration by scodaphoresis. The pre-treatment may comprise, for example, heating or chemical treatment to denature and/or apply a net charge to the protein, binding handle particles to the protein and combinations thereof. High-conductivity samples may be subjected to a conductivity-reduction step to facilitate electrical injection of target particles into scodaphoresis media. The conductivity-reduction step may comprise a buffer exchange process or a salt extraction process, for example. Methods and apparatus can allow two or more different types of target particles to be extracted from the same sample and separately concentrated. These various aspects may be applied individually or in any combination.
摘要翻译: 用于浓缩颗粒的方法和装置可以用于例如浓缩DNA,RNA,蛋白质等。 可以预先处理蛋白质以促进通过scopaphoresis的浓缩。 预处理可以包括例如加热或化学处理以使蛋白质变性和/或施加净电荷,将手段颗粒结合到蛋白质及其组合。 可以对高电导率样品进行电导率降低步骤,以便于将目标颗粒电注射到电泳培养基中。 导电性降低步骤可以包括例如缓冲液交换过程或盐提取过程。 方法和装置可以允许从相同样品中提取两种或更多种不同类型的靶颗粒,并分别浓缩。 这些各个方面可以单独地或以任何组合应用。
-
公开(公告)号:US09211537B2
公开(公告)日:2015-12-15
申请号:US12741880
申请日:2008-11-07
CPC分类号: B01L3/5027 , B01J2219/00317 , B01J2219/00396 , B01J2219/00398 , B01J2219/00495 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/00659 , B01J2219/00722 , B01L3/5025 , B01L3/502723 , B01L3/50273 , B01L3/50851 , B01L7/52 , B01L2200/0605 , B01L2200/0621 , B01L2200/0642 , B01L2200/0689 , B01L2200/16 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/123 , B01L2300/1805 , B01L2400/0487 , G06F3/017 , G06F3/04815 , G06F3/04842
摘要: A microfluidic device comprising a plurality of reaction chambers in fluid communication with a flow channel formed in an elastomeric substrate, a vapor barrier for preventing evaporation from the plurality of reaction chambers, and a continuous phase fluid for isolation of each of the plurality of reaction chambers.
摘要翻译: 一种微流体装置,包括与形成在弹性体基底中的流动通道流体连通的多个反应室,用于防止来自多个反应室的蒸发的蒸汽屏障,以及用于隔离多个反应室中的每一个的连续相流体 。
-
公开(公告)号:US20110053151A1
公开(公告)日:2011-03-03
申请号:US12741880
申请日:2008-11-07
CPC分类号: B01L3/5027 , B01J2219/00317 , B01J2219/00396 , B01J2219/00398 , B01J2219/00495 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/00659 , B01J2219/00722 , B01L3/5025 , B01L3/502723 , B01L3/50273 , B01L3/50851 , B01L7/52 , B01L2200/0605 , B01L2200/0621 , B01L2200/0642 , B01L2200/0689 , B01L2200/16 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/123 , B01L2300/1805 , B01L2400/0487 , G06F3/017 , G06F3/04815 , G06F3/04842
摘要: A microfluidic device comprising a plurality of reaction chambers in fluid communication with a flow channel formed in an elastomeric substrate, a vapor barrier for preventing evaporation from the plurality of reaction chambers, and a continuous phase fluid for isolation of each of the plurality of reaction chambers.
摘要翻译: 一种微流体装置,包括与形成在弹性体基底中的流动通道流体连通的多个反应室,用于防止来自多个反应室的蒸发的蒸汽屏障,以及用于隔离多个反应室中的每一个的连续相流体 。
-
-