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    PROTEIN
    6.
    发明申请
    PROTEIN 有权
    蛋白

    公开(公告)号：US20100086640A1

    公开(公告)日：2010-04-08

    申请号：US12552572

    申请日：2009-09-02

    申请人： Janne Brunstedt ,  Jørn Dalgaard Mikkelsen ,  Henrik Pedersen ,  Jørn Borch Søe

    发明人： Janne Brunstedt ,  Jørn Dalgaard Mikkelsen ,  Henrik Pedersen ,  Jørn Borch Søe

    IPC分类号： A21D13/06 ,  A21D8/04

    CPC分类号： A21D8/042 ,  A23C19/0328 ,  A23D7/02 ,  A23K20/189 ,  A23K50/75 ,  A23L7/107 ,  A23L7/109 ,  A23L7/111 ,  A23L27/60 ,  A23L29/06 ,  C11B3/003 ,  C12N9/18 ,  C12P7/62 ,  C12P19/44 ,  C12Y301/01

    摘要： A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

    摘要翻译： 与甘油三酯相比，极性脂质活性比例更高的真菌野生型脂肪分解酶，其中所述酶优选具有至少为4的磷脂：甘油三酯活性比。优选地，根据本发明的脂肪分解酶具有糖脂： 甘油三酯水解活性比至少为1.5。 在一个实施方案中，根据本发明的真菌脂肪分解酶包含如SEQ ID NO：1或SEQ ID No.2或SEQ ID No.4或SEQ ID No.6所示的氨基酸序列或氨基酸序列， 与其具有至少90％的同一性。 本发明还包括编码真菌脂肪分解酶的核酸，该核酸选自：（a）包含SEQ ID No.3，SEQ ID No.5或SEQ ID No.5所示核苷酸的核酸 第7号 （b）通过遗传密码的退化与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7的核苷酸序列有关的核酸; 和（c）核酸，其包含与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7所示的核苷酸序列具有至少90％同一性的核苷酸序列。

    Fungal lipolytic enzymes, nucleic acids encoding, and uses thereof
    8.
    发明授权
    Fungal lipolytic enzymes, nucleic acids encoding, and uses thereof 有权
    真菌脂肪分解酶，编码的核酸及其用途

    公开(公告)号：US07622290B2

    公开(公告)日：2009-11-24

    申请号：US11519734

    申请日：2006-09-12

    申请人： Janne Brunstedt ,  Jørn Dalgaard Mikkelsen ,  Henrik Pedersen ,  Jørn Borch Søe

    发明人： Janne Brunstedt ,  Jørn Dalgaard Mikkelsen ,  Henrik Pedersen ,  Jørn Borch Søe

    IPC分类号： C12P1/00 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： A21D8/042 ,  A23C19/0328 ,  A23D7/02 ,  A23K20/189 ,  A23K50/75 ,  A23L7/107 ,  A23L7/109 ,  A23L7/111 ,  A23L27/60 ,  A23L29/06 ,  C11B3/003 ,  C12N9/18 ,  C12P7/62 ,  C12P19/44 ,  C12Y301/01

    摘要： A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

    摘要翻译： 与甘油三酯相比，极性脂质活性比例更高的真菌野生型脂肪分解酶，其中所述酶优选具有至少为4的磷脂：甘油三酯活性比。优选地，根据本发明的脂肪分解酶具有糖脂： 甘油三酯水解活性比至少为1.5。 在一个实施方案中，根据本发明的真菌脂肪分解酶包含如SEQ ID NO：1或SEQ ID No.2或SEQ ID No.4或SEQ ID No.6所示的氨基酸序列或氨基酸序列， 与其具有至少90％的同一性。 本发明还包括编码真菌脂肪分解酶的核酸，该核酸选自：（a）包含SEQ ID No.3，SEQ ID No.5或SEQ ID No.5所示核苷酸的核酸 第7号 （b）通过遗传密码的退化与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7的核苷酸序列有关的核酸; 和（c）核酸，其包含与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7所示的核苷酸序列具有至少90％同一性的核苷酸序列。

    Method for reducing 1,2-diglyceride content of an edible oil
    9.
    发明授权
    Method for reducing 1,2-diglyceride content of an edible oil 失效
    降低食用油1,2-二甘油酯含量的方法

    公开(公告)号：US08440435B2

    公开(公告)日：2013-05-14

    申请号：US12492042

    申请日：2009-06-25

    申请人： Paul Wassell ,  Jørn Borch Søe ,  Jørn Dalgaard Mikkelsen ,  Anna Cecilie Jentoft Kristensen

    发明人： Paul Wassell ,  Jørn Borch Søe ,  Jørn Dalgaard Mikkelsen ,  Anna Cecilie Jentoft Kristensen

    IPC分类号： C12P7/64 ,  C12P21/06 ,  C12Q1/48 ,  C11N9/10 ,  C07H21/04

    CPC分类号： C11C1/08 ,  C11C3/06 ,  C12N9/1029 ,  C12P7/6436 ,  C12P7/6445 ,  C12Y203/01

    摘要： The present application provides a method of reducing and/or removing diglyceride from an edible oil, comprising admixing an edible oil with an acyl acceptor substrate and a diglyceride:glycerol acyltransferase, wherein the diglyceride:glycerol acyltransferase is characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol. The diglyceride:glycerol acyltransferase can comprise the amino acid sequence motif GDSX. The present invention also relates to the use of a diglyceride:glycerol acyltransferase in the manufacture of an edible oil, for reducing and/or removing diglyceride from said edible oil and to the use of said enzyme in the manufacture of a foodstuff comprising an edible oil for improving the crystallization properties of said foodstuff.

    摘要翻译： 本申请提供了从食用油中还原和/或除去甘油二酯的方法，包括将食用油与酰基受体底物和甘油二酯：甘油酰基转移酶混合，其中所述甘油二酯：甘油酰基转移酶被表征为可食用的酶 油能够将酰基从甘油二酯转移到甘油。 甘油二酯：甘油酰基转移酶可以包含氨基酸序列基序GDSX。 本发明还涉及甘油二酯：甘油酰基转移酶在制造食用油中的用途，用于从所述食用油中还原和/或除去甘油二酯，以及所述酶在制造包含食用油的食品中的用途 用于改善所述食品的结晶性能。