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公开(公告)号:US20110200596A1
公开(公告)日:2011-08-18
申请号:US13045316
申请日:2011-03-10
IPC分类号: A61K39/395 , C07K16/28 , C12P21/00 , A61P35/00
CPC分类号: C07K16/283 , A61K2039/505 , C07K16/00 , C07K16/2863 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C07K2317/52 , C07K2317/524 , C07K2317/526 , C07K2317/53 , C07K2317/55 , C07K2317/56 , C07K2317/565 , C07K2317/622 , C07K2317/73 , C07K2317/76 , C07K2317/94 , C07K2319/00
摘要: The invention provides methods and compositions comprising a novel stabilized monovalent antibody fragment.
摘要翻译: 本发明提供了包含新的稳定的单价抗体片段的方法和组合物。
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公开(公告)号:US20100028343A1
公开(公告)日:2010-02-04
申请号:US12549497
申请日:2009-08-28
申请人: Jian Chen , Ellen Filvaroff , Sherman Fong , Dorothy French , Audrey Goddard , Paul J. Godowski , J. Christopher Grimaldi , Austin L. Gurney , Kenneth J. Hillan , Sarah G. Hymowitz , Hanzhong Li , James Pan , Melissa A. Starovasnik , Daniel Tumas , Menno Van Lookeren , Richard Vandlen , Colin K. Watanabe , P. Mickey Williams , William I. Wood , Daniel G. Yansura
发明人: Jian Chen , Ellen Filvaroff , Sherman Fong , Dorothy French , Audrey Goddard , Paul J. Godowski , J. Christopher Grimaldi , Austin L. Gurney , Kenneth J. Hillan , Sarah G. Hymowitz , Hanzhong Li , James Pan , Melissa A. Starovasnik , Daniel Tumas , Menno Van Lookeren , Richard Vandlen , Colin K. Watanabe , P. Mickey Williams , William I. Wood , Daniel G. Yansura
IPC分类号: A61K39/395 , A61P19/02
CPC分类号: C07K16/244 , A61K38/00 , C07H21/04 , C07K14/54 , C07K14/7155 , C07K2317/21 , C07K2317/24 , C07K2317/35 , C07K2317/54 , C07K2317/55 , C07K2317/62 , G01N33/505 , G01N33/564 , G01N33/574 , G01N33/57407 , G01N33/57423 , G01N33/57438 , G01N33/57492 , G01N2333/54 , G01N2333/7155
摘要: The present invention is directed to novel polypeptides having sequence identity with IL-17, IL-17 receptors and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided herein are methods for treating degenerative cartilaginous disorders and other inflammatory diseases.
摘要翻译: 本发明涉及与IL-17,IL-17受体和编码那些多肽的核酸分子具有序列同一性的新型多肽。 本文还提供了包含那些核酸序列的载体和宿主细胞,包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子,与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。 本文还提供了治疗退行性软骨疾病和其它炎性疾病的方法。
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公开(公告)号:US5747662A
公开(公告)日:1998-05-05
申请号:US398617
申请日:1995-03-01
IPC分类号: C07K14/245 , C12N15/70 , C07H21/04
CPC分类号: C07K14/245 , C12N15/70
摘要: The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.
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公开(公告)号:US20120058909A1
公开(公告)日:2012-03-08
申请号:US13269984
申请日:2011-10-10
申请人: Jian Chen , Elleri Filvaroff , Sherman Fong , Dorothy French , Audrey Goddard , Paul J. Godowski , J. Christopher Grimaldi , Austin L. Gurney , Kenneth J. Hilian , Sarah G. Hymowitz , Hanzhong Li , James Pan , Melissa A. Starovasnik , Daniel Tumas , Menno Van Lookeren , Richard Vandlen , Colin K. Watanabe , P. Mickey Williams , William I. Wood , Daniel G. Yansura
发明人: Jian Chen , Elleri Filvaroff , Sherman Fong , Dorothy French , Audrey Goddard , Paul J. Godowski , J. Christopher Grimaldi , Austin L. Gurney , Kenneth J. Hilian , Sarah G. Hymowitz , Hanzhong Li , James Pan , Melissa A. Starovasnik , Daniel Tumas , Menno Van Lookeren , Richard Vandlen , Colin K. Watanabe , P. Mickey Williams , William I. Wood , Daniel G. Yansura
IPC分类号: C40B30/04 , G01N33/574 , C12Q1/68
CPC分类号: C07K16/244 , A61K38/00 , C07H21/04 , C07K14/54 , C07K14/7155 , C07K2317/21 , C07K2317/24 , C07K2317/35 , C07K2317/54 , C07K2317/55 , C07K2317/62 , G01N33/505 , G01N33/564 , G01N33/574 , G01N33/57407 , G01N33/57423 , G01N33/57438 , G01N33/57492 , G01N2333/54 , G01N2333/7155
摘要: The present invention is directed to novel polypeptides having sequence identity with IL-17, IL-17 receptors and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided herein are methods for treating degenerative cartilaginous disorders and other inflammatory diseases.
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公开(公告)号:US07029876B2
公开(公告)日:2006-04-18
申请号:US10080866
申请日:2002-02-22
CPC分类号: A61K39/0258 , C07K14/245 , C12N15/70 , C12N15/71 , C12P21/02 , Y02A50/474
摘要: Vectors for producing polypeptides heterologous to prokaryotes are described comprising, along with the polypeptide-encoding nucleic acid, anti-termination nucleic acid that inhibits intragenic transcription termination with a non-lambda promoter therefor and/or nucleic acid encoding a GreA or GreB protein and a promoter therefor. Also described are processes for producing a heterologous polypeptide in prokaryotic host cells utilizing such elements to improve the quality and/or quantity of heterologous polypeptide produced.
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公开(公告)号:US5464756A
公开(公告)日:1995-11-07
申请号:US908766
申请日:1992-07-01
IPC分类号: A61K38/00 , A61P13/02 , A61P15/00 , C07H21/04 , C07K1/113 , C07K1/12 , C07K14/00 , C07K14/575 , C07K14/64 , C12N1/21 , C12N15/09 , C12N15/12 , C12N15/16 , C12P21/00 , C12P21/02 , C12P21/06 , C12R1/19 , C12N15/70
CPC分类号: C07K14/64 , Y10S435/849 , Y10S930/31
摘要: A process is provided for cleaving a polypeptide into at least two polypeptide components comprising treating a reduced, free-cysteine form of the polypeptide with a cleaving agent under conditions for cleaving the polypeptide at a desired junction between the polypeptide cleavage products. More preferably, the process for cleaving comprises culturing cells containing DNA encoding said polypeptide, wherein at least one Asp codon is present in said DNA at a desired junction between the components to be cleaved from each other, said culturing resulting in expression of the DNA to produce the polypeptide in the host cell culture; and treating a reduced, free-cysteine form of the polypeptide with dilute acid under conditions for cleaving the polypeptide at the Asp junction. In particular embodiments, a DNA sequence is provided that encodes a relaxin precursor and includes codons encoding aspartic acid-containing linkers at novel positions within the precursor, allowing the ready cleavage of relaxin A peptides by treatment with dilute acid.
摘要翻译: 提供了一种用于将多肽切割成至少两种多肽组分的方法,包括在多肽切割产物之间的所需连接处切割多肽的条件下用切割剂处理还原的游离半胱氨酸形式的多肽。 更优选地,所述切割方法包括培养含有编码所述多肽的DNA的细胞,其中至少一个Asp密码子存在于所述DNA中在待切割的组分之间的所需连接处,所述培养导致DNA的表达 在宿主细胞培养物中产生多肽; 以及在Asp连接处切割多肽的条件下用稀酸处理还原的游离半胱氨酸形式的多肽。 在具体实施方案中,提供了编码松弛素前体的DNA序列,并且包括在前体内的新位置编码含有天冬氨酸的接头的密码子,从而允许通过用稀酸处理来快速切割松弛素A肽。
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公开(公告)号:US5411873A
公开(公告)日:1995-05-02
申请号:US928697
申请日:1992-08-11
摘要: Processes for producing various heterologous polypeptides which when expressed are either incorrectly processed and hence asssociated with the surface of the host cell or are not processed to mature form. More specifically, processes for the production of heterologous non-human carbonyl hydrolases expressed either in host cells incapable of producing enzymatically active endoprotease or host cells deficient in enzymatically active extracellular endoprotease are disclosed. Such non-human carbonyl hydrolases generally are incapable of autoproteolytic maturation and become associated with the surface of expression hosts which are deficient in enzymatically active extracellular endoprotease. Processes for preparing non-human carbonyl hydrolase and heterologous polypeptides which are expressed as part of a fusion polypeptide are also disclosed, as well as non-human carbonyl hydrolases which are substantially free of the host cell membrane with which they are normally associated.
摘要翻译: 用于产生各种异源多肽的方法,当被表达时,它们被错误地加工并因此与宿主细胞的表面相结合或者不被加工成成熟形式。 更具体地,公开了在不能产生酶促活性内切蛋白酶的宿主细胞中表达的异源非人羰基水解酶的生产方法或在酶活性细胞外内蛋白酶中缺乏的宿主细胞。 这种非人羰基水解酶通常不能自身蛋白水解成熟,并且与酶活性细胞外内蛋白酶缺陷的表达宿主的表面相关联。 还公开了制备非人羰基水解酶和作为融合多肽的一部分表达的异源多肽的方法,以及基本上不含与它们通常结合的宿主细胞膜的非人羰基水解酶。
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公开(公告)号:US4803164A
公开(公告)日:1989-02-07
申请号:US42604
申请日:1987-04-22
CPC分类号: C07K14/005 , A61K39/12 , A61K39/292 , C12N15/81 , C12N2730/10122 , C12N2730/10134
摘要: Hepatitis surface antigen is synthesized in recombinant yeast hosts transformed with vectors encoding hepatitis surface antigen, preferably under the control of the yeast PGK promoter and preferably in the absence of DNA encoding the surface antigen precursor. Hepatitis surface antigen is assembled by yeast into antigenic 22 nm particles even though hepatitis surface antigen bacterial transformants were not known to be capable of assembling the surface antigen into 22 nm particles.
摘要翻译: 在用编码肝炎表面抗原的载体转化的重组酵母宿主中合成肝炎表面抗原,优选在酵母PGK启动子的控制下,优选在不存在编码表面抗原前体的DNA的情况下。 尽管肝炎表面抗原细菌转化体不能将表面抗原组装成22纳米的颗粒,但肝炎表面抗原由酵母组装成抗原性22nm的颗粒。
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公开(公告)号:US20130064827A1
公开(公告)日:2013-03-14
申请号:US13593362
申请日:2012-08-23
申请人: Jian Chen , Ellen Filvaroff , Sherman Fong , Dorothy French , Audrey Goddard , Paul J. Godowski , J. Christopher Grimaldi , Austin L. Gumey , Kenneth J. Hillan , Sarah G. Hymowitz , Hanzhong Li , James Pan , Melissa A. Starovasnik , Daniel Tumas , Menno Van Lookeren , Richard Vandlen , Colin K. Watanabe , P. Mickey Williams , William I. Wood , Daniel G. Yansura
发明人: Jian Chen , Ellen Filvaroff , Sherman Fong , Dorothy French , Audrey Goddard , Paul J. Godowski , J. Christopher Grimaldi , Austin L. Gumey , Kenneth J. Hillan , Sarah G. Hymowitz , Hanzhong Li , James Pan , Melissa A. Starovasnik , Daniel Tumas , Menno Van Lookeren , Richard Vandlen , Colin K. Watanabe , P. Mickey Williams , William I. Wood , Daniel G. Yansura
IPC分类号: A61K39/395 , C07K16/24 , C07H21/02 , A61P19/04 , A61P29/00 , C07H21/04 , A61K31/7088
CPC分类号: C07K16/244 , A61K38/00 , C07H21/04 , C07K14/54 , C07K14/7155 , C07K2317/21 , C07K2317/24 , C07K2317/35 , C07K2317/54 , C07K2317/55 , C07K2317/62 , G01N33/505 , G01N33/564 , G01N33/574 , G01N33/57407 , G01N33/57423 , G01N33/57438 , G01N33/57492 , G01N2333/54 , G01N2333/7155
摘要: The present invention is directed to novel polypeptides having sequence identity with IL-17, IL-17 receptors and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided herein are methods for treating degenerative cartilaginous disorders and other inflammatory diseases.
摘要翻译: 本发明涉及与IL-17,IL-17受体和编码那些多肽的核酸分子具有序列同一性的新型多肽。 本文还提供了包含那些核酸序列的载体和宿主细胞,包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子,与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。 本文还提供了治疗退行性软骨疾病和其它炎性疾病的方法。
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10.
公开(公告)号:US06242177B1
公开(公告)日:2001-06-05
申请号:US08397303
申请日:1995-03-01
IPC分类号: C12Q168
CPC分类号: C12N15/625 , C07K2319/02 , C07K2319/036 , C07K2319/61 , C07K2319/75 , C12N15/70
摘要: The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.
摘要翻译: 本发明公开了意想不到的结果,与野生型信号序列相比,具有降低的翻译强度的突变信号序列提供了基本上完整的处理和高水平的感兴趣的多肽的表达,并且许多哺乳动物多肽需要较窄的翻译水平范围 达到最大分泌。 一组信号序列载体提供一系列翻译强度,以优化感兴趣的多肽的表达。
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