公开(公告)号：US06787017B1

公开(公告)日：2004-09-07

申请号：US09806913

申请日：2001-04-06

申请人： Atsumu Hirabayashi ,  Yukiko Hirabayashi ,  Akihiko Okumura ,  Hideaki Koizumi

发明人： Atsumu Hirabayashi ,  Yukiko Hirabayashi ,  Akihiko Okumura ,  Hideaki Koizumi

IPC分类号： G01N27447

CPC分类号： G01N27/44791 ,  G01N27/44704

摘要： The capillary electrophoresis system includes a wafer-shaped part having passages filled with a buffer solution for introducing sample solutions; a body having a configuration suitable to removably hold and to move the wafer-shaped part. The body includes first and second electrodes for applying a voltage across both ends of passages of the wafer-shaped part to separate and remove the sample solution. The body also includes first and second buffer reservoirs conductive to passages of the wafer-shaped part at specific positions for filling buffer solution around the first and second electrodes. The system according to the present invention facilitates washing of electrophoresis passages and reduces the time and labor costs required for replacement of the fused-silica wafers and simplifies operation by allowing a plurality of sample solutions to be analyzed more quickly.

摘要翻译： 毛细管电泳系统包括具有填充有用于引入样品溶液的缓冲溶液的通道的晶片状部件; 具有适于可移除地保持和移动晶片形部分的构造的本体。 本体包括第一和第二电极，用于跨晶片形部分的通道的两端施加电压以分离和去除样品溶液。 身体还包括第一和第二缓冲储存器，其导电到晶片状部分在特定位置处的通道，用于在第一和第二电极周围填充缓冲溶液。 根据本发明的系统有助于电泳通道的洗涤，并且减少了更换熔凝石英晶片所需的时间和人力成本，并且通过允许更快速地分析多个样品溶液来简化操作。

公开(公告)号：US06774359B1

公开(公告)日：2004-08-10

申请号：US09762276

申请日：2001-02-06

申请人： Yukiko Hirabayashi ,  Atsumu Hirabayashi ,  Akihiko Okumura ,  Hideaki Koizumi

发明人： Yukiko Hirabayashi ,  Atsumu Hirabayashi ,  Akihiko Okumura ,  Hideaki Koizumi

IPC分类号： G01N2306

CPC分类号： H01J49/04 ,  B01F5/0413 ,  B01F13/0059 ,  B01L3/5027 ,  G01N35/085

摘要： In a sample-introduction tool in which an end of a passage is connected to a reservoir for storing a mobile phase solution, a sample-dropping orifice is provided at the passage and the mobile phase solution and a sample running off from the other end of the passage are introduced into a mass spectrometer by generating a high-speed gas stream around the other end of the passage. The mobile phase solution and the sample are nebulized by the high-speed gas stream, and the injected sample is ionized.

摘要翻译： 在其中通道的端部连接到用于存储流动相溶液的储存器的样品引入工具中，在通道和流动相溶液处提供了一个抽样孔，并且从另一端流出的样品 通过在通道的另一端周围产生高速气流将通道引入质谱仪。 流动相溶液和样品被高速气流雾化，注入的样品离子化。

公开(公告)号：US06478738B1

公开(公告)日：2002-11-12

申请号：US09695019

申请日：2000-10-25

申请人： Yukiko Hirabayashi ,  Atsumu Hirabayashi ,  Hideaki Koizumi

发明人： Yukiko Hirabayashi ,  Atsumu Hirabayashi ,  Hideaki Koizumi

IPC分类号： A61B500

CPC分类号： H01J49/0431 ,  A61B5/145 ,  A61B5/14528 ,  A61B5/14557 ,  A61B5/1459 ,  A61B5/4064 ,  A61B2010/0077

摘要： A living body fluid mass-analyzing method introducing a first solution whose osmotic pressure is close to that of a living body fluid into a microdialysis, extracting the living body fluid from the microdialysis, mixing the living body fluid from the microdialysis and an organic solution, and applying a gas around a capillary through which the mixed living fluid flows thereby spraying and ionizing the living body fluid by the gas. Further, the ionized ions are introduced into three dimensional mass-analyzer through an orifice, accumulating the ions in said three dimensional mass-analyzer and sending out the accumulated ions in order of mass to measure mass numbers of the ions.

摘要翻译： 一种生物体液分析方法，其将渗透压接近生物体液的第一溶液引入微透析，从微透析提取生物体液，混合来自微透析的活体液和有机溶液， 并且在毛细管周围施加气体，混合的生物流体通过该毛细管喷射并通过气体电离生物体液。 此外，通过孔口将离子化离子引入三维质量分析器中，将离子积聚在所述三维质量分析器中，并以质量的顺序排出积聚的离子以测量离子的质量数。

公开(公告)号：US6114693A

公开(公告)日：2000-09-05

申请号：US299638

申请日：1999-04-27

申请人： Atsumu Hirabayashi ,  Yukiko Hirabayashi ,  Minoru Sakairi ,  Yasuaki Takada ,  Takayuki Nabeshima ,  Hideaki Koizumi

发明人： Atsumu Hirabayashi ,  Yukiko Hirabayashi ,  Minoru Sakairi ,  Yasuaki Takada ,  Takayuki Nabeshima ,  Hideaki Koizumi

IPC分类号： G01N27/62 ,  G01N27/447 ,  G01N30/72 ,  H01J49/04 ,  H01J49/10

CPC分类号： G01N30/7266 ,  H01J49/0468 ,  H01J49/167

摘要： A mass spectrometer comprising a passage through which a sample solution flows, the passage including a capillary having an open tip, the sample solution flowing through the capillary out of the open tip of the capillary, a gas passage which forms a gas flow around the open tip of the capillary, the gas flow spraying the sample solution flowing out of the open tip of the capillary, a first electrode which contacts the sample solution flowing in the passage and applies an electric potential to the sample solution flowing in the passage, a second electrode disposed around the capillary near the open tip of the capillary, the second electrode being downstream from the first electrode with respect to a direction in which the sample solution flows in the passage, there being no gap extending downstream from the open tip of the capillary to the second electrode in a direction in which the sample solution flows out of the open tip of the capillary, the second electrode being electrically isolated from the sample solution flowing in the capillary and applying an electric field to the sample solution flowing in the capillary, and an analyzer which analyzes a mass of gaseous ions formed from the sample solution sprayed by the gas flow.

摘要翻译： 一种质谱仪，包括样品溶液流过的通道，所述通道包括具有开口尖端的毛细管，所述样品溶液从毛细管的开口尖端流过毛细管，气体通道围绕开口形成气流 毛细管的尖端，气流喷射从毛细管的开口尖端流出的样品溶液，第一电极，其接触在通道中流动的样品溶液，并向在通道中流动的样品溶液施加电位，第二电极 设置在毛细管附近的毛细管周围的电极，第二电极相对于样品溶液在通道中流动的方向从第一电极下游，在毛细管的开口尖端的下游没有间隙 在样品溶液从毛细管的开口尖端流出的方向到第二电极，第二电极是电隔离的 从在毛细管中流动的样品溶液流出并向在毛细管中流动的样品溶液施加电场，以及分析器，其分析由气流喷射的样品溶液形成的气态离子的质量。

公开(公告)号：US06190316B1

公开(公告)日：2001-02-20

申请号：US09273554

申请日：1999-03-22

申请人： Yukiko Hirabayashi ,  Atsumu Hirabayashi ,  Hideaki Koizumi

发明人： Yukiko Hirabayashi ,  Atsumu Hirabayashi ,  Hideaki Koizumi

IPC分类号： A61B500

CPC分类号： H01J49/0431 ,  A61B5/145 ,  A61B5/14528 ,  A61B5/14557 ,  A61B5/1459 ,  A61B5/4064 ,  A61B2010/0077

摘要： A living body fluid analyzing system includes a microdialysis for sending a first solution having an osmotic pressure which is substantially similar to a osmotic pressure of a body fluid into a living body and extracting a second solution from the living body. A first flow passage is provided in which the second solution from the microdialysis flows and a second flow passage is provided which mixes the second solution with an organic solution. Furthermore, there is provided a gas source and a gas flow controller which controls a flow quantity of the gas from the gas source and a third flow passage in which a gas introduced from the gas source flows. An ion source is provided having an orifice for spraying and ionizing the second solution from the second flow passage at an end of the third flow passage, and a mass spectrometer is provided for mass-analyzing the ions sprayed from the orifice.

摘要翻译： 活体液分析系统包括微透析，用于将具有基本上类似于体液渗透压的渗透压的第一溶液送入生物体并从生物体提取第二溶液。 提供第一流动通道，其中来自微量透析的第二溶液流动，并且提供将第二溶液与有机溶液混合的第二流动通道。 此外，提供了气体源和气体流量控制器，其控制来自气体源的气体的流量和从气体源引入的气体流过的第三流路。 提供离子源，其具有用于在第三流动通道的端部处从第二流动通道喷射和离子化第二溶液的孔，并且提供质量分析器以对从孔喷射的离子进行质量分析。

公开(公告)号：US5898175A

公开(公告)日：1999-04-27

申请号：US708415

申请日：1996-09-05

申请人： Atsumu Hirabayashi ,  Yukiko Hirabayashi ,  Minoru Sakairi ,  Yasuaki Takada ,  Takayuki Nabeshima ,  Hideaki Koizumi

发明人： Atsumu Hirabayashi ,  Yukiko Hirabayashi ,  Minoru Sakairi ,  Yasuaki Takada ,  Takayuki Nabeshima ,  Hideaki Koizumi

IPC分类号： G01N27/62 ,  G01N27/447 ,  G01N30/72 ,  H01J49/04 ,  H01J49/10

CPC分类号： G01N30/7266 ,  H01J49/0468 ,  H01J49/167

摘要： A mass spectrometer employing an ion source includes a capillary for spraying therethrough liquid into the atmospheres and an orifice through which a portion in the vicinity of a tip of the capillary is inserted, the orifice being designed in such a way that gases are caused to flow up to the tip of the capillary along a peripheral wall face of the capillary. The flow rate of gas in the vicinity of the tip of the capillary is about the sonic velocity, and a voltage is applied across the liquid flowing through the capillary and the orifice or an electrode arranged in the vicinity of the tip of the capillary.

摘要翻译： 使用离子源的质谱仪包括用于将液体喷射到气氛中的毛细管和插入毛细管的尖端附近的部分的孔，孔被设计成使气体流动 直到毛细管的尖端沿着毛细管的周壁面。 毛细管尖端附近的气体的流速大约是声速，并且在流过毛细管的液体和布置在毛细管尖端附近的孔或电极之间施加电压。

公开(公告)号：US06437327B2

公开(公告)日：2002-08-20

申请号：US09859508

申请日：2001-05-18

申请人： Yasuaki Takada ,  Minoru Sakairi ,  Atsumu Hirabayashi ,  Takayuki Nabeshima ,  Hideaki Koizumi

发明人： Yasuaki Takada ,  Minoru Sakairi ,  Atsumu Hirabayashi ,  Takayuki Nabeshima ,  Hideaki Koizumi

IPC分类号： H01J4910

CPC分类号： H01J49/165 ,  G01N30/7266 ,  H01J49/168

摘要： A mass spectrometer including a sprayer for spraying a sample solution, a needle electrode for ionizing the sample contained in the sample solution, an aperture for introducing the ions of the sample into a lower pressure region having a pressure lower than the pressure of a region in which the needle electrode is disposed, and a mass spectrometric apparatus for analyzing the ions introduced from the aperture. A direction of a spray caused by the sprayer is almost perpendicular to or at an angle with respect to a center axis of the aperture, and a tip of the needle is disposed facing to an electrode having the aperture.

摘要翻译： 一种质谱仪，包括用于喷射样品溶液的喷雾器，用于使包含在样品溶液中的样品电离的针状电极，用于将样品的离子引入低于区域压力的低压区域的孔 设置针状电极的质谱仪和用于分析从孔径引入的离子的质谱仪。 由喷雾器引起的喷雾方向几乎与孔的中心轴垂直或成一定角度，并且针的尖端面向具有孔的电极。

公开(公告)号：US06188065B1

公开(公告)日：2001-02-13

申请号：US09260552

申请日：1999-03-02

申请人： Yasuaki Takada ,  Minoru Sakairi ,  Atsumu Hirabayashi ,  Hideaki Koizumi

发明人： Yasuaki Takada ,  Minoru Sakairi ,  Atsumu Hirabayashi ,  Hideaki Koizumi

IPC分类号： G01D5944

CPC分类号： H01J49/145 ,  H01J49/0431 ,  H01J49/049 ,  H01J49/165 ,  H01J49/168

摘要： A mass spectrometer includes a sample supplier which supplies a sample solution, the sample solution including a solvent, ions, and a solute, the solute being a sample to be analyzed, an ion converter, disposed after the sample supplier, which converts the ions in the sample solution into gaseous ions, an ion source, disposed after the ion converter, which ionizes the sample in the sample solution, thereby producing sample ions, a mass analyzer which analyzes masses of the sample ions produced by the ion source, and an ion blocking electrode which prevents the gaseous ions produced by the ion converter from reaching the ion source, thereby preventing the mass analyzer from analyzing masses of the gaseous ions produced by the ion converter.

摘要翻译： 质谱仪包括供样品溶液的样品供应商，包含溶剂的样品溶液，离子和溶质，作为待分析样品的溶质，设置在样品供应者之后的离子转化器，其将离子转化为 将样品溶液变成气态离子，离子源，设置在离子转换器之后，将样品溶液中的样品离子化，从而产生样品离子;分析质量分析器，其分析由离子源产生的样品离子的质量;以及离子 阻止由离子转换器产生的气体离子到达离子源的阻挡电极，从而防止质量分析器分析由离子转换器产生的气态离子的质量。

公开(公告)号：US6121608A

公开(公告)日：2000-09-19

申请号：US968898

申请日：1997-11-06

申请人： Yasuaki Takada ,  Minoru Sakairi ,  Atsumu Hirabayashi ,  Takayuki Nabeshima ,  Hideaki Koizumi

发明人： Yasuaki Takada ,  Minoru Sakairi ,  Atsumu Hirabayashi ,  Takayuki Nabeshima ,  Hideaki Koizumi

IPC分类号： G01N27/62 ,  B01D59/44 ,  G01N30/72 ,  H01J49/00 ,  H01J49/04 ,  H01J49/10

CPC分类号： H01J49/165 ,  G01N30/7266 ,  H01J49/168

摘要： A mass spectrometric apparatus includes a device for supplying a sample solution to an outlet, the sample solution including a solvent and a solute, a first ionization device for receiving the sample solution from the outlet and electrospraying the received sample solution, thereby ionizing at least a portion of the received sample solution, a second ionization device for receiving at least a portion of the electrosprayed sample solution produced by the first ionization device and ionizing at least a portion of the received electrospayed sample solution, thereby producing ions, and a mass spectrometric device for receiving at least some of the ions produced by the second ionization device and analyzing masses of the received ions.

摘要翻译： 质谱装置包括用于将样品溶液供应到出口的装置，所述样品溶液包括溶剂和溶质，用于从所述出口接收样品溶液并对所接收的样品溶液进行电喷雾的第一离子化装置，从而将至少一种 接收样品溶液的一部分，第二电离装置，用于接收由第一离子化装置产生的电喷雾样品溶液的至少一部分，并且电离至少一部分接收的电喷雾样品溶液，从而产生离子，以及质谱仪 用于接收由第二电离装置产生的至少一些离子并分析所接收的离子的质量。

公开(公告)号：US5877495A

公开(公告)日：1999-03-02

申请号：US511804

申请日：1995-08-07

申请人： Yasuaki Takada ,  Minoru Sakairi ,  Atsumu Hirabayashi ,  Hideaki Koizumi

发明人： Yasuaki Takada ,  Minoru Sakairi ,  Atsumu Hirabayashi ,  Hideaki Koizumi

IPC分类号： G01N27/62 ,  G01N27/447 ,  H01J49/04 ,  B01D59/44 ,  H01J49/00

CPC分类号： H01J49/145 ,  H01J49/0431 ,  H01J49/049 ,  H01J49/165 ,  H01J49/168

摘要： A mass spectrometer comprising a capillary electrophoresis region for separating a solution containing molecules of sample by capillary electrophoresis in a capillary, a nebulization region for nebulizing the solution containing molecules of the sample under an atmospheric pressure from the end of the capillary and forming liquid droplets of the solution containing the molecules of the sample, a vaporization region for vaporizing the liquid droplets under an atmospheric pressure to form gaseous molecules of the sample, a chemical ionization region for forming ions relevant to the molecules of the sample under the atmospheric pressure or the reduced pressure by a chemical reaction between the ions attributable to the gaseous molecules present in the atmosphere and the gaseous molecules of the sample, and a vacuum region having a sample aperture for introducing the ions formed by the chemical ionization means and incorporating a mass analysis region for mass analysis of ions introduced from the sample aperture. The mass spectrometer is combined with the capillary electrophoresis apparatus and is particularly suitable to formation and mass analysis of ions relevant to the neutral molecules of the sample.

摘要翻译： 一种质谱仪，其包括用于通过毛细管中的毛细管电泳分离含有样品分子的溶液的毛细管电泳区域，用于在大气压下从毛细管端部喷雾含有样品分子的溶液的雾化区域，并形成液滴 含有样品分子的溶液，用于在大气压下蒸发液滴的汽化区域以形成样品的气态分子;用于在大气压下形成与样品分子相关的离子的化学电离区域，或减少 存在于大气中的气体分子的离子和样品的气体分子之间的化学反应产生的压力，以及具有用于引入由化学电离装置形成的离子的样品孔的真空区域，并且包含用于 离子质量分析介绍f 取样孔。 质谱仪与毛细管电泳装置结合，特别适用于与样品中性分子相关的离子的形成和质量分析。