公开(公告)号：US11731129B2

公开(公告)日：2023-08-22

申请号：US17357372

申请日：2021-06-24

申请人： BERKELEY LIGHTS, INC.

发明人： Paul M. Lundquist ,  Paul M. Lebel ,  Phillip Ronald Thomas Jess

IPC分类号： B01L3/00 ,  B01L7/00 ,  G01J3/02 ,  G01J3/10 ,  G01J3/44 ,  G01N21/31 ,  G01N21/64 ,  G02B21/06 ,  G02B21/16 ,  G02B21/36 ,  G01N1/40

CPC分类号： B01L3/502715 ,  B01L7/00 ,  G01J3/0208 ,  G01J3/0229 ,  G01J3/10 ,  G01J3/4406 ,  G01N1/40 ,  G01N21/31 ,  G01N21/6458 ,  G02B21/06 ,  G02B21/16 ,  G02B21/361 ,  B01L2200/147 ,  B01L2300/046 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2400/0424

摘要： The present disclosure relates to an optical apparatus for imaging and/or manipulating micro-objects in a microfluidic device, such as a light-actuated microfluidic (LAMF) device, and related systems and methods. The optical apparatus can comprise a structured light modulator, a first and a second tube lens, an objective lens, a dichroic beam splitter, and an image sensor. The structured light modulator can be configured to receive unstructured light beams and transmit structured light beams for illuminating micro-objects located within an enclosure of the microfluidic device and/or selectively activating one or more of a plurality of dielectrophoresis (DEP) electrodes of the microfluidic device. The image light beams received by the image sensor can be used to form an image of at least a portion of the microfluidic device.

公开(公告)号：US10829728B2

公开(公告)日：2020-11-10

申请号：US16010176

申请日：2018-06-15

申请人： Berkeley Lights, Inc.

发明人： Volker L. S. Kurz ,  Troy A. Lionberger ,  Eric K. Sackmann ,  Kai W. Szeto ,  Paul M. Lebel ,  Brandon R. Bruhn ,  Keith J. Breinlinger ,  Eric D. Hobbs ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC分类号： B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/26

摘要： Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

公开(公告)号：US11789016B2

公开(公告)日：2023-10-17

申请号：US16849811

申请日：2020-04-15

申请人： Berkeley Lights, Inc.

发明人： Troy A. Lionberger ,  Phillip J. M. Elms ,  Anupam Singhal ,  Randall D. Lowe, Jr. ,  Volker L. S. Kurz ,  Paul M. Lebel

IPC分类号： B01L3/00 ,  G01N33/543 ,  G01N33/68

CPC分类号： G01N33/54366 ,  B01L3/502761 ,  G01N33/6854 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/165 ,  B01L2300/1822 ,  B01L2400/0424 ,  B01L2400/0457 ,  B01L2400/0472 ,  B01L2400/086

摘要： Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

公开(公告)号：US20210270817A1

公开(公告)日：2021-09-02

申请号：US17198833

申请日：2021-03-11

申请人： Berkeley Lights, Inc.

发明人： Paul M. Lebel ,  Troy A. Lionberger ,  Kevin T. Chapman

IPC分类号： G01N33/53 ,  B01L3/00

摘要： Disclosed herein is a method for assaying binding affinity between a first molecule and a second molecule in a micro-fluidic device.

公开(公告)号：US11802264B2

公开(公告)日：2023-10-31

申请号：US17067971

申请日：2020-10-12

申请人： Berkeley Lights, Inc.

发明人： Volker L. S. Kurz ,  Troy A. Lionberger ,  Eric K. Sackmann ,  Kai W. Szeto ,  Paul M. Lebel ,  Brandon R. Bruhn ,  Keith J. Breinlinger ,  Eric D. Hobbs ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC分类号： B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/26 ,  G01N15/14

CPC分类号： C12M23/16 ,  B01L3/502761 ,  C12M23/20 ,  C12M33/12 ,  B01L2200/0668 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0442 ,  B01L2400/0454 ,  B01L2400/086

摘要： Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

公开(公告)号：US11077438B2

公开(公告)日：2021-08-03

申请号：US16415894

申请日：2019-05-17

申请人： Berkeley Lights, Inc.

发明人： Paul M. Lundquist ,  Paul M. Lebel ,  Phillip Ronald Thomas Jess

IPC分类号： B01L3/00 ,  B01L7/00 ,  G01J3/02 ,  G01J3/10 ,  G01J3/44 ,  G01N1/40 ,  G01N21/31 ,  G01N21/61 ,  G02B21/06 ,  G02B21/16 ,  G02B21/36 ,  G01N21/64

摘要： The present disclosure relates to an optical apparatus for imaging and/or manipulating micro-objects in a microfluidic device, such as a light-actuated microfluidic (LAMF) device, and related systems and methods. The optical apparatus can comprise a structured light modulator, a first and a second tube lens, an objective lens, a dichroic beam splitter, and an image sensor. The structured light modulator can be configured to receive unstructured light beams and transmit structured light beams for illuminating micro-objects located within an enclosure of the microfluidic device and/or selectively activating one or more of a plurality of dielectrophoresis (DEP) electrodes of the microfluidic device. The first tube lens can be configured to capture the structured light beams transmitted by the structured light modulator. The second tube lens can be configured to transmit image light beams from the dichroic beam splitter to the image sensor. The image sensor can be configured to receive image light beams from the second tube lens. The image light beams received by the image sensor can be used to form an image of at least a portion of the microfluidic device.

公开(公告)号：US20210102150A1

公开(公告)日：2021-04-08

申请号：US17067971

申请日：2020-10-12

申请人： Berkeley Lights, Inc.

发明人： Volker L.S. Kurz ,  Troy A. Lionberger ,  Eric K. Sackmann ,  Kai W. Szeto ,  Paul M. Lebel ,  Brandon R. Bruhn ,  Keith J. Breinlinger ,  Eric D. Hobbs ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC分类号： C12M3/06 ,  B01L3/00 ,  C12M1/00 ,  C12M1/26

摘要： Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

公开(公告)号：US20180298318A1

公开(公告)日：2018-10-18

申请号：US16010176

申请日：2018-06-15

申请人： Berkeley Lights, Inc.

发明人： Volker L.S. Kurz ,  Troy A. Lionberger ,  Erik K. Sackmann ,  Kai W. Szeto ,  Paul M. Lebel ,  Brandon R. Bruhn ,  Keith J. Breinlinger ,  Eric D. Hobbs ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC分类号： C12M3/06 ,  B01L3/00 ,  C12M1/00 ,  C12M1/26

CPC分类号： C12M23/16 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0442 ,  B01L2400/0454 ,  B01L2400/086 ,  C12M23/20 ,  C12M33/12

摘要： Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.