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1.发明授权Astaxanthin-producing yeast cells, methods for their preparation and their use 失效产生虾青素的酵母细胞，其制备方法及其应用
公开(公告)号：US5972642A
公开(公告)日：1999-10-26
申请号：US989860
申请日：1997-12-12
申请人： Bent Flen.o slashed. , Ib Christensen , Robert Larsen , Steffen Radich Johansen , Eric A. Johnson
发明人： Bent Flen.o slashed. , Ib Christensen , Robert Larsen , Steffen Radich Johansen , Eric A. Johnson
IPC分类号： A23C19/00 , A23D7/005 , A23J1/18 , A23K20/179 , A23K50/80 , A23L5/44 , A23L5/46 , A23L17/00 , C12N1/16 , C12P23/00 , C12R1/645
CPC分类号： C12R1/645 , A23D7/0053 , A23D7/0056 , A23J1/18 , A23K20/179 , A23K50/80 , A23L17/70 , A23L5/44 , A23L5/46 , C12P23/00
摘要： An isolated pure culture of a strain of the yeast Phaffia rhodozyma which produces large amounts of astaxanthin, in excess of about 600 micrograms per gram of Phaffia rhodozyma dry matter, as determined by HPLC analysis, is disclosed.
摘要翻译： 公开了通过HPLC分析测定的，产生大量虾青素的酵母红酵母菌的分离的纯培养物，其超过约600微克/克红发夫酵母干物质。
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2.发明授权
公开(公告)号：US5712110A
公开(公告)日：1998-01-27
申请号：US484683
申请日：1995-06-07
申请人： Bent Flen.o slashed. , Ib Christensen , Robert Larsen , Steffen Radich Johansen , Eric A. Johnson
发明人： Bent Flen.o slashed. , Ib Christensen , Robert Larsen , Steffen Radich Johansen , Eric A. Johnson
摘要： An isolated pure culture of a strain of Phaffia rhodozyma which produces astaxanthin in an amount of at least 600 .mu.g per g Phaffia rhodozyma dry matter, as determined by HPLC analysis.
摘要翻译： 通过HPLC分析测定的红精原酵母菌株的分离的纯培养物，其产生至少600μg/ g红发夫酵母干物质的虾青素。
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3.发明授权Astaxanthin-producing yeast cells, methods for their preparation and their use 失效产生虾青素的酵母细胞，其制备方法及其应用
公开(公告)号：US5709856A
公开(公告)日：1998-01-20
申请号：US478292
申请日：1995-06-07
申请人： Bent Flen.o slashed. , Ib Christensen , Robert Larsen , Steffen Radich Johansen , Eric A. Johnson
发明人： Bent Flen.o slashed. , Ib Christensen , Robert Larsen , Steffen Radich Johansen , Eric A. Johnson
IPC分类号： A23J1/18 , A23K1/16 , A23K1/18 , A23L1/275 , A23L1/325 , C12P23/00 , A61K35/72 , A23L1/28 , C12N1/16
摘要： An isolated pure culture of a strain of Phaffia rhodozyma which produces astaxanthin in an amount of at least 600 .mu.g per g Phaffia rhodozyma dry matter, as determined by HPLC analysis.
摘要翻译： 通过HPLC分析测定的红精原酵母菌株的分离的纯培养物，其产生至少600μg/ g红发夫酵母干物质的虾青素。
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4.发明授权Astaxanthin-producing yeast cells, methods for their preparation and their use 失效产生虾青素的酵母细胞，其制备方法及其应用
公开(公告)号：US5679567A
公开(公告)日：1997-10-21
申请号：US322690
申请日：1994-10-13
申请人： Bent Flen.o slashed. , Ib Christensen , Robert Larsen , Steffen Radich Johansen , Eric A. Johnson
发明人： Bent Flen.o slashed. , Ib Christensen , Robert Larsen , Steffen Radich Johansen , Eric A. Johnson
摘要： An isolated pure culture of a strain of Phaffia rhodozyma which produces astaxanthin in an mount of at least 600 .mu.g per g Phaffia rhodozyma dry matter, as determined by HPLC analysis.
摘要翻译： 通过HPLC分析测定的红精原红酵母菌的分离纯培养物，其产生至少600μg/ g红发夫酵母干物质的虾青素。
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5.发明授权Astaxanthin-producing yeast cells, methods for their preparation and their use 失效产生虾青素的酵母细胞，其制备方法及其应用
公开(公告)号：US5599711A
公开(公告)日：1997-02-04
申请号：US322950
申请日：1994-10-13
摘要： An isolated pure culture of a strain of Phaffia rhodozyma which produces astaxanthin in an amount of at least 600 .mu.g per g Phaffia rhodozyma dry matter, as determined by HPLC analysis.
摘要翻译： 通过HPLC分析测定的红精原酵母菌株的分离的纯培养物，其产生至少600μg/ g红发夫酵母干物质的虾青素。
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6.发明授权Astaxanthin-producing yeast cells, methods for their preparation and their use 失效产生虾青素的酵母细胞，其制备方法及其应用
公开(公告)号：US5356810A
公开(公告)日：1994-10-18
申请号：US919986
申请日：1992-07-27
摘要： An isolated pure culture of a strain of Phaffia rhodozyma which produces astaxanthin in an amount of at least 600 .mu.g per g Phaffla rhodozyma dry matter, as determined by HPLC analysis.
摘要翻译： 通过HPLC分析测定的红精原酵母菌的分离纯培养物，其产生至少600μg/ g Phaffla rhodozyma干物质的虾青素。
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公开(公告)号：US20140255376A1
公开(公告)日：2014-09-11
申请号：US14181241
申请日：2014-02-14
申请人： Eric A. Johnson , Sabine Pellett , William H. Tepp , Tony L. Yaksh , Marc Marino , Qinghao Xu
发明人： Eric A. Johnson , Sabine Pellett , William H. Tepp , Tony L. Yaksh , Marc Marino , Qinghao Xu
CPC分类号： A61K47/62 , A61K9/0085 , A61K38/164 , A61K38/4893 , C07K7/22 , C07K14/33 , C07K14/57545 , C07K14/70 , C07K14/72 , C07K2319/01 , C07K2319/33 , C07K2319/55 , C12N9/96 , C12Y304/24069
摘要： A chimeric toxin is disclosed comprising a peptide ligand specifically targeting neurons involved in pain processing; and a clostridial neurotoxin light chain, wherein the ligand is linked to the light chain. The methods of preparing such chimeric toxin and the method of using the chimeric toxin to regulate pain transmission are also disclosed.
摘要翻译： 公开了一种嵌合毒素，其包含特异性靶向涉及疼痛加工的神经元的肽配体; 和梭菌神经毒素轻链，其中所述配体与轻链连接。 还公开了制备这种嵌合毒素的方法和使用嵌合毒素调节疼痛传递的方法。
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8.发明授权Plasmid-encoded neurotoxin genes in Clostridium botulinum serotype A subtypes 有权肉毒毒素肉毒杆菌血清型A亚型中的质粒编码的神经毒素基因公开(公告)号：US08435759B2
公开(公告)日：2013-05-07
申请号：US12664800
申请日：2008-06-27
CPC分类号： C12N9/52 , C07K14/33 , C12N15/74 , C12Y304/24069
摘要： The present invention provides a novel isolated plasmid, wherein the plasmid is a native plasmid found in unique C. botulinum type A strains and encode either BoNT/A3 or BoNT/A4 and BoNT/B. The present invention also provides a method of obtaining a plasmid-encoded botulinum neurotoxin and botulinum neurotoxin complex comprising the step of isolating a plasmid encoding the cntA/A or cntA/B neurotoxin gene and genes encoding protein components of the toxin complex from a C. botulinum type A strain. The inventors performed comparative analyzes of representative BoNT/A subtype strains by pulsed-field gel electrophoresis (PFGE) and Southern hybridizations with probes specific for the BoNT/A and B genes, cntA/A and cntA/B. Unexpectedly, the inventors determined that the genes encoding BoNT/A3 in the A3 strain, and BoNT/A4 and BoNT/B in the A4 strain, are on plasmids.
摘要翻译： 本发明提供了一种新的分离质粒，其中质粒是在独特的A型肉毒杆菌中发现的天然质粒，并且编码BoNT / A3或BoNT / A4和BoNT / B。 本发明还提供了获得质粒编码的肉毒杆菌神经毒素和肉毒杆菌神经毒素复合物的方法，包括分离编码cntA / A或cntA / B神经毒素基因的质粒和编码来自C的毒素复合物的蛋白质组分的基因的步骤。 A型肉毒杆菌。 本发明人通过脉冲场凝胶电泳（PFGE）和针对BoNT / A和B基因cntA / A和cntA / B特异性探针的Southern杂交进行了代表性的BoNT / A亚型菌株的比较分析。 出人意料的是，本发明人确定了A3菌株中编码BoNT / A3的基因以及A4菌株中的BoNT / A4和BoNT / B在质粒上。
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9.发明授权Passive alignment of VCSELs to waveguides in opto-electronic cards and printed circuit boards 失效将VCSELs无源对准到光电卡和印刷电路板中的波导公开(公告)号：US07499614B2
公开(公告)日：2009-03-03
申请号：US10692921
申请日：2003-10-24
申请人： Eric A. Johnson , David V. Caletka
发明人： Eric A. Johnson , David V. Caletka
IPC分类号： G02B6/26
CPC分类号： G02B6/1221 , G02B6/12004 , G02B6/42 , G02B6/4232 , G02B2006/12104 , G02B2006/12176
摘要： A method of producing opto-electronic cards and printed circuit boards which are adapted to provide for passive alignment of VCSELs to waveguides. Also provided are opto-electronic cards and printed circuit boards which incorporate structure providing for the passive alignment of VCSELs to waveguides.
摘要翻译： 一种制造光电卡和印刷电路板的方法，其适于提供VCSEL与波导的无源对准。 还提供了光电卡和印刷电路板，其结合提供VCSEL到波导的被动对准的结构。
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公开(公告)号：US20090017495A1
公开(公告)日：2009-01-15
申请号：US12040542
申请日：2008-02-29
申请人： Michael Baldwin , Eric A. Johnson , Joseph T. Barbieri , Marite Bradshaw , William H. Tepp , Christina L. Pier
发明人： Michael Baldwin , Eric A. Johnson , Joseph T. Barbieri , Marite Bradshaw , William H. Tepp , Christina L. Pier
CPC分类号： C12N9/52 , A61K39/0258 , A61K39/08 , A61K2039/523 , C12Y304/24069 , Y02A50/469 , Y02A50/474
摘要： A method of producing botulinum toxin C-terminal receptor binding domain (HCR) is disclosed. The one embodiment, the method comprises the steps of (a) preparing E. coli transformed with an expression vector comprising DNA encoding HCR protein, (b) inducing expression of the HCR protein at a reduced temperature in a culture media, and (c) purifying the HCR protein via extraction, wherein the extraction comprises a clarification by centrifugation and a filtration, wherein the purified HCR protein is at least 10 mg/L of culture medium.
摘要翻译： 公开了产生肉毒杆菌毒素C末端受体结合结构域（HCR）的方法。 该方法包括以下步骤：（a）用包含编码HCR蛋白的DNA的表达载体转化大肠杆菌，（b）在培养基中降低温度诱导HCR蛋白的表达，和（c） 通过提取纯化HCR蛋白质，其中提取物通过离心和过滤进行澄清，其中纯化的HCR蛋白质为至少10mg / L的培养基。
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