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9 条记录 (耗时 0.04 秒)

    Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis
    6.
    发明授权
    Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis 失效
    克隆和表达重组粘蛋白Mefp-1的蓝色贻贝，Mytilus edulis

    公开(公告)号：US06987170B1

    公开(公告)日：2006-01-17

    申请号：US10915161

    申请日：2004-08-09

    申请人： Heather G. Silverman ,  Francisco F. Roberto

    发明人： Heather G. Silverman ,  Francisco F. Roberto

    IPC分类号： C07K17/00 ,  C12P21/06

    CPC分类号： C07K14/43504 ,  C07K5/1013

    摘要： The present invention comprises a Mytilus edulis cDNA sequenc having a nucleotide sequence that encodes for the Mytilus edulis foot protein-1 (Mefp-1), an example of a mollusk foot protein. Mefp-1 is an integral component of the blue mussels' adhesive protein complex, which allows the mussel to attach to objects underwater. The isolation, purification and sequencing of the Mefp-1 gene will allow researchers to produce Mefp-1 protein using genetic engineering techniques. The discovery of Mefp-1 gene sequence will also allow scientists to better understand how the blue mussel creates its waterproof adhesive protein complex.

    摘要翻译： 本发明包括具有编码Mytilus edulis足蛋白-1（Mefp-1）的核苷酸序列的麦鞭草cDNA序列，其为软体动物足部蛋白质的实例。 Mefp-1是蓝贻贝粘合蛋白复合物的组成部分，可使贻贝附着于水下物体。 Mefp-1基因的分离，纯化和测序将使研究人员能够使用遗传工程技术生产Mefp-1蛋白。 Mefp-1基因序列的发现也将使科学家更好地了解蓝贻贝如何产生其防水粘合蛋白复合物。

    Microbial production of epoxides
    7.
    发明授权
    Microbial production of epoxides 失效

    公开(公告)号：US06576449B2

    公开(公告)日：2003-06-10

    申请号：US09805795

    申请日：2001-03-12

    申请人： Thomas R. Clark ,  Francisco F. Roberto

    发明人： Thomas R. Clark ,  Francisco F. Roberto

    IPC分类号： C12P700

    CPC分类号： C12P17/02 ,  C12N1/005 ,  Y10S435/822

    摘要： A method for microbial production of epoxides and other oxygenated products is disclosed. The method uses a biocatalyst of methanotrophic bacteria cultured in a biphasic medium containing a major amount of a non-aqueous polar solvent. Regeneration of reducing equivalents is carried out by using endogenous hydrogenase activity together with supplied hydrogen gas. This method is especially effective with gaseous substrates and cofactors that result in liquid products.

    Cloning and expression of recombinant adhesive protein MEFP-2 of the blue mussel, Mytilus edulis
    8.
    发明授权
    Cloning and expression of recombinant adhesive protein MEFP-2 of the blue mussel, Mytilus edulis 失效
    克隆和表达重组粘蛋白MEFP-2的蓝色贻贝，Mytilus edulis

    公开(公告)号：US06995012B1

    公开(公告)日：2006-02-07

    申请号：US10915160

    申请日：2004-08-09

    申请人： Heather G. Silverman ,  Francisco F. Roberto

    发明人： Heather G. Silverman ,  Francisco F. Roberto

    IPC分类号： C12N15/00

    CPC分类号： C07H21/04 ,  C07K14/43504

    摘要： The present invention includes a Mytilus edulis cDNA having a nucleotide sequence that encodes for the Mytilus edulis foot protein-2 (Mefp-2), an example of a mollusk foot protein. Mefp-2 is an integral component of the blue mussels' adhesive protein complex, which allows the mussel to attach to objects underwater. The isolation, purification and sequencing of the Mefp-2 gene will allow researchers to produce Mefp-2 protein using genetic engineering techniques. The discovery of Mefp-2 gene sequences will also allow scientists to better understand how the blue mussel creates its waterproof adhesive protein complex.

    摘要翻译： 本发明包括具有编码Mytilus edulis foot protein-2（Mefp-2）的核苷酸序列的麦鞭草cDNA，其是软体动物足部蛋白质的实例。 Mefp-2是蓝色贻贝粘合蛋白复合物的组成部分，可以使贻贝附着在水下的物体上。 Mefp-2基因的分离，纯化和测序将使研究人员能够使用遗传工程技术生产Mefp-2蛋白。 Mefp-2基因序列的发现也将使科学家更好地了解蓝贻贝如何产生其防水粘合蛋白复合物。

    Method for the decontamination of soil containing solid organic explosives therein
    9.
    发明授权
    Method for the decontamination of soil containing solid organic explosives therein 失效
    其中含有固体有机炸药的土壤去污的方法

    公开(公告)号：US6051420A

    公开(公告)日：2000-04-18

    申请号：US82421

    申请日：1998-05-20

    申请人： Corey W. Radtke ,  Francisco F. Roberto

    发明人： Corey W. Radtke ,  Francisco F. Roberto

    IPC分类号： B09C1/10 ,  C12N1/20 ,  C12N1/26 ,  C12N1/32 ,  C12S9/00 ,  C12S13/00 ,  B09B3/00

    CPC分类号： C12N1/20 ,  B09C1/10 ,  C12N1/26 ,  C12N1/32 ,  Y02W30/43 ,  Y02W30/47

    摘要： An efficient method for decontaminating soil containing organic explosives ("TNT" and others) in the form of solid portions or chunks which are not ordinarily subject to effective bacterial degradation. The contaminated soil is treated by delivering an organic solvent to the soil which is capable of dissolving the explosives. This process makes the explosives more bioavailable to natural bacteria in the soil which can decompose the explosives. An organic nutrient composition is also preferably added to facilitate decomposition and yield a compost product. After dissolution, the explosives are allowed to remain in the soil until they are decomposed by the bacteria. Decomposition occurs directly in the soil which avoids the need to remove both the explosives and the solvents (which either evaporate or are decomposed by the bacteria). Decomposition is directly facilitated by the solvent pre-treatment process described above which enables rapid bacterial remediation of the soil.

    摘要翻译： 一种有效的方法，用于净化含有有机爆炸物（“TNT”等）的土壤，其形式为固体部分或块，通常不会有效地进行细菌降解。 污染的土壤通过将有机溶剂输送到能够溶解爆炸物的土壤来处理。 这个过程使得爆炸物对土壤中能分解爆炸物的天然细菌更具生物利用度。 还优选加入有机营养组合物以促进分解并产生堆肥产物。 溶解后，允许爆炸物留在土壤中直至细菌分解。 分解直接发生在土壤中，避免需要去除爆炸物和溶剂（其被蒸发或被细菌分解）。 通过上述溶剂预处理方法直接促进分解，其能够快速对土壤进行细菌修复。