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公开(公告)号：US20210394186A1
公开(公告)日：2021-12-23
申请号：US17289636
申请日：2019-10-29
申请人： BRANDEIS UNIVERSITY
发明人： S. Ali Aghvami , Seth Fraden
IPC分类号： B01L3/00
摘要： Systems and methods are provided for producing isolated microfluidic droplets. In one aspect, a microfluidic system comprises a droplet isolation device and an injection system. The droplet isolation device includes at least one isolation unit and at least one capillary valve. The isolation unit has at least one chamber configured to receive at least two different aqueous solutions without mixing prior to entering the at least one chamber based at least in part on pressure levels of the at least two different aqueous solutions. The injection system includes an aqueous inlet, a non-aqueous inlet, a bypass outlet, a working fluid outlet, and a loading chamber. The injection system is configured to allow for a predetermined amount of each of the at least two different aqueous solutions to be delivered to the droplet isolation device sequentially.
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公开(公告)号：US20210164870A1
公开(公告)日：2021-06-03
申请号：US17170022
申请日：2021-02-08
申请人： BRANDEIS UNIVERSITY
发明人： Seth Fraden , Michael Heymann , Markus Ludwig
IPC分类号： G01N1/28 , B01L3/00 , B01D9/00 , G01N23/20025 , C12M3/00
摘要： Microfluidic devices and methods for investigating crystallization and/or for controlling a reaction or a phase transition are disclosed. In one embodiment, the microfluidic device includes a reservoir layer; a membrane disposed on the reservoir layer; a wetting control layer disposed on the membrane; and a storage layer disposed on the wetting control layer, wherein the wetting control layer and the storage layer define a microfluidic channel comprising an upstream portion, a downstream portion, a first fluid path in communication with the upstream and the downstream portions, and a storage well positioned within the first fluid path, wherein the wetting control layer includes a fluid passageway in communication with the storage well and the membrane, and wherein the wetting control layer wets a first fluid introduced into the microfluidic channel, the first fluid comprising a hydrophilic, lipophilic, fluorophilic or gas phase as the continuous phase in the microfluidic channel.
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公开(公告)号：US09017623B2
公开(公告)日：2015-04-28
申请号：US14294737
申请日：2014-06-03
申请人： Brandeis University
CPC分类号： B01L3/502784 , B01L3/06 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L7/54 , B01L2200/0621 , B01L2200/0642 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/1822 , B01L2400/0406 , B01L2400/0655 , C30B7/00 , C30B29/58 , G01N1/38 , G01N2001/386 , Y10T137/0318 , Y10T436/2575
摘要： Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions.
摘要翻译： 提供微流体结构和操纵流体和反应的方法。 这样的结构和方法可以涉及在微流体网络中的预定区域中将例如液滴形式的流体样品定位在载体流体（例如，可能与流体样品不混溶的油）中。 在一些实施例中，液滴的定位可以按照它们被引入微流体网络（例如，顺序地）而没有液滴之间的显着物理接触的顺序进行。 由于液滴之间很少或没有接触，所以液滴之间可能很少或没有聚结。 因此，在一些这样的实施方案中，在流体样品或载体流体中不需要表面活性剂以防止液滴的聚结。 本文所述的结构和方法还使得能够从预定区域顺序地去除液滴。
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公开(公告)号：US20240042445A1
公开(公告)日：2024-02-08
申请号：US18489364
申请日：2023-10-18
申请人： Brandeis University
CPC分类号： B01L3/502784 , C30B7/00 , C30B29/58 , G01N1/38 , B01L3/502746 , B01L3/502715 , B01L3/50273 , Y10T436/2575 , Y10T137/0318 , B01L3/06
摘要： Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions.
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公开(公告)号：US10960397B2
公开(公告)日：2021-03-30
申请号：US16916532
申请日：2020-06-30
发明人： Seth Fraden , Hakim Boukellal , Yanwei Jia , Seila Selimovic , Amy Rowat , Jeremy Agresti , David A. Weitz
摘要： Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.
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公开(公告)号：US10942095B2
公开(公告)日：2021-03-09
申请号：US16447369
申请日：2019-06-20
申请人： BRANDEIS UNIVERSITY
发明人： Seth Fraden , Michael Heymann , Markus Ludwig
摘要： Microfluidic devices and methods for investigating crystallization and/or for controlling a reaction or a phase transition are disclosed. In one embodiment, the microfluidic device includes a reservoir layer; a membrane disposed on the reservoir layer; a wetting control layer disposed on the membrane; and a storage layer disposed on the wetting control layer, wherein the wetting control layer and the storage layer define a microfluidic channel comprising an upstream portion, a downstream portion, a first fluid path in communication with the upstream and the downstream portions, and a storage well positioned within the first fluid path, wherein the wetting control layer includes a fluid passageway in communication with the storage well and the membrane, and wherein the wetting control layer wets a first fluid introduced into the microfluidic channel, the first fluid comprising a hydrophilic, lipophilic, fluorophilic or gas phase as the continuous phase in the microfluidic channel.
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公开(公告)号：US20200269248A1
公开(公告)日：2020-08-27
申请号：US16834505
申请日：2020-03-30
申请人： Brandeis University
摘要： Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions.
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公开(公告)号：US10675626B2
公开(公告)日：2020-06-09
申请号：US16573466
申请日：2019-09-17
发明人： Seth Fraden , Hakim Boukellal , Yanwei Jia , Seila Selimovic , Amy Rowat , Jeremy Agresti , David A. Weitz
摘要： Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.
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公开(公告)号：US10357772B2
公开(公告)日：2019-07-23
申请号：US16105283
申请日：2018-08-20
发明人： Seth Fraden , Hakim Boukellal , Yanwei Jia , Seila Selimovic , Amy Rowat , Jeremy Agresti , David A. Weitz
摘要： Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.
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公开(公告)号：US11819849B2
公开(公告)日：2023-11-21
申请号：US16834505
申请日：2020-03-30
申请人： Brandeis University
CPC分类号： B01L3/502784 , B01L3/50273 , B01L3/502715 , B01L3/502746 , C30B7/00 , C30B29/58 , G01N1/38 , B01L3/06 , B01L3/5027 , B01L7/54 , B01L2200/0621 , B01L2200/0642 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/1822 , B01L2400/0406 , B01L2400/0655 , G01N2001/386 , Y10T137/0318 , Y10T436/2575
摘要： Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions.
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