公开(公告)号：US5154887A

公开(公告)日：1992-10-13

申请号：US705874

申请日：1991-05-28

申请人： Bruce E. Babb ,  Fred T. Oakes

发明人： Bruce E. Babb ,  Fred T. Oakes

IPC分类号： C09B55/00 ,  G01N33/533

CPC分类号： G01N33/533 ,  C09B55/009 ,  Y10S435/805

摘要： Certain phenalenimine fluorescent compounds represented by the structure ##STR1## wherein R' and R" are independently hydrogen, alkyl, cycloalkyl, aryl or a heterocycle, or R' comprises the carbon and heteroatoms which form a fused ring with the compound nucleus, are useful in biomedical and analytical determinations. These compounds can be used for staining cells, as well as for the determination of various analytes found in human or animal biological fluids. Such determinations can be carried out in solution or by using dry analytical elements. The fluorescent compounds can be reacted with quinone nuclei to form reducible compounds which are also useful in analytical methods. In addition, the compounds can be incorporated into what are known as "loadable" latex particles to form detectable labels and biological reagents.

摘要翻译： 由结构表示的某些吩噻嗪类荧光化合物，其中R'和R“独立地是氢，烷基，环烷基，芳基或杂环，或R'包括与化合物核形成稠合环的碳和杂原子 可用于生物医学和分析测定。 这些化合物可用于染色细胞，以及用于测定在人或动物生物流体中发现的各种分析物。 这种测定可以在溶液中或通过使用干燥的分析元件进行。 荧光化合物可与醌核反应形成可还原的化合物，也可用于分析方法。 此外，化合物可以掺入所谓的“可装载”胶乳颗粒中以形成可检测的标记物和生物试剂。

公开(公告)号：US5055414A

公开(公告)日：1991-10-08

申请号：US215478

申请日：1988-07-05

申请人： Bruce E. Babb ,  Fred T. Oakes

发明人： Bruce E. Babb ,  Fred T. Oakes

IPC分类号： G01N33/533 ,  C07C251/20 ,  C07D213/74 ,  C07D221/18 ,  C09B55/00 ,  C09B57/00 ,  C09K11/07 ,  G01N33/58

CPC分类号： C07D213/74 ,  C07C251/20 ,  C07D221/18 ,  C09B55/009 ,  C09B57/00 ,  G01N33/582 ,  C07C2101/14 ,  C07C2103/28 ,  Y10S436/80 ,  Y10S436/805

摘要： Certain phenalenimine fluorescent compounds represented by the structure ##STR1## wherein R' and R" are independently hydrogen, alkyl, cycloalkyl, aryl or a heterocycle, or R' comprises the carbon and heteroatoms which forms a fused ring with the compound nucleus, are useful in biomedical and analytical determinations. These compounds can be used for staining cells, as well as for the determination of various analytes found in human or animal biological fluids. Such determination can be carried out in solution or by using dry analytical elements. The fluorescent compounds can be reacted with quinone nuclei to form reducible compounds which are also useful in analytical methods. In addition, the compounds can be incorporated into what are known as "loadable" latex particles to form detectable labels and biological reagents.

公开(公告)号：US4670385A

公开(公告)日：1987-06-02

申请号：US612509

申请日：1984-05-21

申请人： Bruce E. Babb ,  Daniel S. Daniel

发明人： Bruce E. Babb ,  Daniel S. Daniel

IPC分类号： G01N33/50 ,  C12Q1/00 ,  C12Q1/28

CPC分类号： C12Q1/28 ,  C12Q2326/40 ,  Y10S435/805 ,  Y10T436/206664

摘要： A particular class of triarylmethane leuco dyes are useful in analytical compositions, elements and methods. These leuco dyes are triarylmethane compounds having an aromatic heterocyclic moiety attached to the central methane carbon and which, upon interaction with hydrogen peroxide, provide dyes having a maximum absorption at a wavelength equal to or greater than about 600 nm. Particularly useful leuco dyes have the structure: ##STR1## wherein R is a 5- to 15-membered aromatic heterocyclic moiety; and R' and R" are independently open chain or cyclic amines. These compounds are useful for providing detectable dyes in compositions and elements useful for detection of hydrogen peroxide or other analytes which react to produce hydrogen peroxide in aqueous liquids, e.g. biological fluids.

摘要翻译： 特定类型的三芳基甲烷无色染料可用于分析组合物，元素和方法。 这些无色染料是具有连接到中心甲烷碳上的芳族杂环部分的三芳基甲烷化合物，并且在与过氧化氢相互作用时提供在等于或大于约600nm的波长处具有最大吸收的染料。 特别有用的无色染料具有以下结构：其中R是5至15元芳族杂环部分; 且R'和R“独立地为开链或环状胺。 这些化合物可用于在用于检测过氧化氢或其它分析物的组合物和元件中提供可检测的染料，所述分析物在含水液体中产生过氧化氢反应。 生物体液。

公开(公告)号：US4795712A

公开(公告)日：1989-01-03

申请号：US37303

申请日：1987-04-10

申请人： John L. Toner ,  Bruce J. Murray ,  Bruce E. Babb ,  Michael W. Sundberg

发明人： John L. Toner ,  Bruce J. Murray ,  Bruce E. Babb ,  Michael W. Sundberg

IPC分类号： C09B23/14 ,  C09B29/36 ,  C09B57/00 ,  C09B57/06 ,  C09B57/10 ,  C09B69/00 ,  G01N33/84 ,  G01N31/22

CPC分类号： C09B23/145 ,  C09B29/36 ,  C09B57/06 ,  C09B57/10 ,  C09B69/00 ,  G01N33/84 ,  G01N2800/04

摘要： Novel chromogenic derivatives of 1,2-bis-(o-aminoaryloxy)ethane-N,N,N',N'-tetraacetic acid are useful for the determination of calcium ions in both solution and dry assays. These compounds comprise a dye moiety which is directly conjugated to the acetic acid substituted-nitrogen atom and which enables the compounds to exhibit maximum absorbance at a wavelength generally greater than 400 nm before complexation. When the compounds are complexed with calcium ions, the absorbance shifts to a shorter wavelength.

公开(公告)号：US5215925A

公开(公告)日：1993-06-01

申请号：US716587

申请日：1991-06-17

申请人： Bruce E. Babb ,  David A. Hilborn

发明人： Bruce E. Babb ,  David A. Hilborn

IPC分类号： C07F3/00 ,  G01N31/22 ,  G01N33/84

CPC分类号： C07C317/00 ,  C07F3/003 ,  G01N33/84

摘要： 1,5-Bis(2-hydroxyphenyl)-3-cyanoformazans substituted in at least one of the 3-, 4- and 5-positions of either phenyl moiety are useful for the determination of magnesium ions. The cyanoformazan substituents are chosen such that their cumulative Hammett-sigma value is greater than about 0.23. These cyanoformazans are useful in analytical compositions and methods for either wet or dry assay of magnesium ions.

摘要翻译： 在苯基部分的3-，4-和5-位中的至少一个中取代的1,5-双（2-羟基苯基）-3-氰基甲硅烷可用于测定镁离子。 选择氰基甲脒取代基使得它们的累积哈米特 - 西格玛值大于约0.23。 这些氰甲酸可用于镁离子的湿法或干法测定的分析组合物和方法。

公开(公告)号：US4812409A

公开(公告)日：1989-03-14

申请号：US824756

申请日：1986-01-31

申请人： Bruce E. Babb ,  Robert T. Belly ,  Patricia M. Scensny

发明人： Bruce E. Babb ,  Robert T. Belly ,  Patricia M. Scensny

IPC分类号： C12Q1/34 ,  C07C67/00 ,  C07C69/017 ,  C07C233/09 ,  C07C233/33 ,  C07C233/61 ,  C07C233/76 ,  C07C325/00 ,  C07C327/20 ,  C07H13/06 ,  C07H15/20 ,  C07K14/00 ,  C09B3/00 ,  C09B3/78 ,  C09B57/00 ,  C09K11/06 ,  C12Q1/44 ,  G01N33/52 ,  G01N33/53

CPC分类号： G01N33/52 ,  C09B3/00 ,  C09B57/00 ,  C12Q1/44 ,  C12Q2334/00 ,  Y10S436/80

摘要： Hydrolyzable substrates comprise blocked dye moieties which, when cleaved from the substrate during hydrolysis, provide fluorescent dyes having maximum absorptions above about 530 nm and maximum emissions at least about 580 nm at physiological pH. These substrates can be used in analytical determinations of hydrolytic substances including hydrolytic enzymes or biological cells containing such enzymes.

摘要翻译： 可水解底物包括封闭的染料部分，其在水解期间从底物切割时，提供在生理pH下具有高于约530nm的最大吸收和最大发射至少约580nm的荧光染料。 这些底物可用于水解物质的分析测定，包括水解酶或含有这些酶的生物细胞。

公开(公告)号：US4803161A

公开(公告)日：1989-02-07

申请号：US824757

申请日：1986-01-31

申请人： Bruce E. Babb ,  Robert T. Belly ,  Albert J. Mura

发明人： Bruce E. Babb ,  Robert T. Belly ,  Albert J. Mura

IPC分类号： C12Q1/26 ,  C07C45/00 ,  C07C49/727 ,  C07C49/747 ,  C07C67/00 ,  C07C213/00 ,  C07C225/22 ,  C07C271/50 ,  C07C271/54 ,  C07C313/00 ,  C07C323/22 ,  C09B3/78 ,  C09B57/00 ,  C09K11/07 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/32 ,  G01N21/76 ,  G01N31/22 ,  G01N33/04 ,  G01N33/52 ,  G01N33/72 ,  C12Q1/02

CPC分类号： C07C271/50 ,  C07C271/54 ,  C12Q1/04 ,  G01N33/52 ,  C12Q2304/20 ,  C12Q2304/26 ,  G01N33/04 ,  Y10S436/80 ,  Y10S436/805

摘要： Certain phenalenone and benzphenalenone fluorescent compounds are useful in biomedical studies and analytical determinations. These compounds are particularly useful in assays for living organisms, e.g. microorganisms, carried out at a pH of 9 or less. For these determinations, the compounds can be attached to reducible compounds which will subsequently release the fluorescent moiety upon reduction. In the presence of an electron transfer agent, the reducible compounds are easily reduced by a microorganism.

摘要翻译： 某些phenalenone和benzphenalenone荧光化合物可用于生物医学研究和分析测定。 这些化合物特别可用于生物体的测定，例如 微生物在pH为9以下进行。 对于这些测定，化合物可以连接到可还原的化合物上，随后在还原时将其释放荧光部分。 在电子转移剂的存在下，可还原的化合物容易被微生物还原。

公开(公告)号：US4751178A

公开(公告)日：1988-06-14

申请号：US780508

申请日：1985-09-26

申请人： Bruce E. Babb ,  Roy E. Snoke

发明人： Bruce E. Babb ,  Roy E. Snoke

IPC分类号： C07D215/12 ,  C07D217/14 ,  C07D217/18 ,  C09B23/14 ,  C12Q1/48 ,  G01N33/52

CPC分类号： C09B23/145 ,  C12Q1/48 ,  Y10S435/805

摘要： New substrates for .gamma.-glutamyltransferase (.gamma.-GT) are disclosed. These subtrates are useful in the colorimetric determination of .gamma.-GT since the cleavage of the .gamma.-glutamyl group produces a detectable species--an aminostyrylquinolinium dye. The substrates have the formula: ##STR1## wherein: R and R.sub.1 are independently selected from hydrogen, alkyl and alkoxy andQ is ##STR2## wherein: R.sub.2 is alkyl having from 1 to 4 carbon atoms;R.sub.3 and R.sub.4 are independently selected from hydrogen, alkyl, alkoxy, nitro, cyano and halogen; andX.sup..crclbar. is an anion.

摘要翻译： 公开了γ-谷氨酰转移酶（γ-GT）的新底物。 由于γ-谷氨酰基的裂解产生可检测的物质 - 氨基苯乙烯基喹啉染料，这些底物可用于γ-GT的比色测定。 底物具有下式：其中：R和R 1独立地选自氢，烷基和烷氧基，Q是其中：R 2是具有1至4个碳原子的烷基; R 3和R 4独立地选自氢，烷基，烷氧基，硝基，氰基和卤素; 而X（ - ）是阴离子。

公开(公告)号：US4555483A

公开(公告)日：1985-11-26

申请号：US505049

申请日：1983-06-17

申请人： Charles M. LiMuti ,  Bruce E. Babb ,  John C. Mauck

发明人： Charles M. LiMuti ,  Bruce E. Babb ,  John C. Mauck

IPC分类号： C12N9/20 ,  C12Q1/44 ,  C12N9/18 ,  C12R1/125

CPC分类号： C12N9/20 ,  C12Q1/44 ,  Y10S435/81 ,  Y10S435/839

摘要： A method for the determination of the amount of lipase in a sample comprises the steps of:(a) contacting the sample with a reagent composition comprising:(i) a lipase substrate which is a glycerol triester oil having in one of its two .alpha.-ester positions a long chain alkyl group having at least 8 carbon atoms and, in its two remaining ester positions, short chain alkyl groups such that, if the long chain alkyl group is hydrolyzed, the resulting diester is water soluble; and(ii) an esterase enzyme capable of catalyzing the hydrolysis of a water soluble glycerol diester to glycerol; and(b) detecting the rate at which glycerol is formed.The compositions of the present invention include the substrate and enzyme as defined. The element of the invention comprises a support having thereon the described composition. The invention is useful for determining lipase in samples which contain endogenous glycerol, such as blood serum and other body fluids.

摘要翻译： 用于测定样品中脂肪酶量的方法包括以下步骤：（a）使样品与试剂组合物接触，所述试剂组合物包含：（i）脂肪酶底物，其是甘油三酯油，其具有其两个α- 酯位置具有至少8个碳原子的长链烷基，并且在其两个剩余的酯位置中具有短链烷基，使得如果长链烷基被水解，则所得二酯是水溶性的; 和（ii）能够催化水溶性甘油二酯水解成甘油的酯酶; 和（b）检测形成甘油的速率。 本发明的组合物包括如所定义的底物和酶。 本发明的元件包括其上具有所述组合物的载体。 本发明可用于测定含有内源甘油（如血清和其他体液）的样品中的脂肪酶。

公开(公告)号：US4468467A

公开(公告)日：1984-08-28

申请号：US344433

申请日：1982-02-01

申请人： Bruce E. Babb ,  Glen M. Dappen

发明人： Bruce E. Babb ,  Glen M. Dappen

IPC分类号： C07C67/00 ,  C07C241/00 ,  C07C245/20 ,  C07C301/00 ,  C07C311/37 ,  G01N33/72 ,  C07C113/00

CPC分类号： G01N33/728 ,  Y10S436/903 ,  Y10T436/146666

摘要： Diazonium salts which are useful in an assay for bilirubin are disclosed. The novel salts have the structural formula: ##STR1## wherein X.sup.- is a stabilizing anion, Y is CO-- or SO.sub.2 --; and R.sup.1 and R.sup.2 are independently selected from hydrogen, alkyl, aryl, carboxyalkyl and hydroxyalkyl providing R.sup.1 and R.sup.2 are not both hydrogen; R.sup.3 and R.sup.4 are independently selected from groups such that the sum of the Hammett sigma values for R.sup.3 and R.sup.4 does not exceed +0.4 or R.sup.3 and R.sup.4 represent the carbon atoms necessary to complete a fused carbocyclic ring structure. Reagent compositions, analytical elements and methods which use the described diazonium salt are also disclosed.

摘要翻译： 公开了可用于胆红素测定的重氮盐。 新型盐具有以下结构式：其中X是稳定阴离子，Y是CO-或SO2-; 并且R 1和R 2独立地选自氢，烷基，芳基，羧基烷基和羟烷基，提供R1和R2不同时为氢; R3和R4独立地选自以下的基团，使得R 3和R 4的Hammettσ值的总和不超过+0.4或R3和R4表示完成稠合的碳环结构所必需的碳原子。 还公开了使用所述重氮盐的试剂组合物，分析元件和方法。