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14 条记录 (耗时 0.018 秒)

    Methods, compositions and elements for the determination of lipase
    1.
    发明授权
    Methods, compositions and elements for the determination of lipase 失效
    用于测定脂肪酶的方法，组成和元素

    公开(公告)号：US4555483A

    公开(公告)日：1985-11-26

    申请号：US505049

    申请日：1983-06-17

    申请人： Charles M. LiMuti ,  Bruce E. Babb ,  John C. Mauck

    发明人： Charles M. LiMuti ,  Bruce E. Babb ,  John C. Mauck

    IPC分类号： C12N9/20 ,  C12Q1/44 ,  C12N9/18 ,  C12R1/125

    CPC分类号： C12N9/20 ,  C12Q1/44 ,  Y10S435/81 ,  Y10S435/839

    摘要： A method for the determination of the amount of lipase in a sample comprises the steps of:(a) contacting the sample with a reagent composition comprising:(i) a lipase substrate which is a glycerol triester oil having in one of its two .alpha.-ester positions a long chain alkyl group having at least 8 carbon atoms and, in its two remaining ester positions, short chain alkyl groups such that, if the long chain alkyl group is hydrolyzed, the resulting diester is water soluble; and(ii) an esterase enzyme capable of catalyzing the hydrolysis of a water soluble glycerol diester to glycerol; and(b) detecting the rate at which glycerol is formed.The compositions of the present invention include the substrate and enzyme as defined. The element of the invention comprises a support having thereon the described composition. The invention is useful for determining lipase in samples which contain endogenous glycerol, such as blood serum and other body fluids.

    摘要翻译： 用于测定样品中脂肪酶量的方法包括以下步骤：（a）使样品与试剂组合物接触，所述试剂组合物包含：（i）脂肪酶底物，其是甘油三酯油，其具有其两个α- 酯位置具有至少8个碳原子的长链烷基，并且在其两个剩余的酯位置中具有短链烷基，使得如果长链烷基被水解，则所得二酯是水溶性的; 和（ii）能够催化水溶性甘油二酯水解成甘油的酯酶; 和（b）检测形成甘油的速率。 本发明的组合物包括如所定义的底物和酶。 本发明的元件包括其上具有所述组合物的载体。 本发明可用于测定含有内源甘油（如血清和其他体液）的样品中的脂肪酶。

    Use of dry analytical elements to determine analytes
    2.
    发明授权
    Use of dry analytical elements to determine analytes 失效
    使用干燥的分析元素来确定分析物

    公开(公告)号：US5047322A

    公开(公告)日：1991-09-10

    申请号：US251494

    申请日：1988-09-30

    申请人： Robert E. Emmons ,  Linda A. Mauck ,  John C. Mauck ,  TaiWing Wu ,  Royden N. Rand ,  Angelo P. Andrese

    发明人： Robert E. Emmons ,  Linda A. Mauck ,  John C. Mauck ,  TaiWing Wu ,  Royden N. Rand ,  Angelo P. Andrese

    IPC分类号： C12Q1/00 ,  G01N27/447 ,  G01N33/52

    CPC分类号： C12Q1/00 ,  G01N27/44726 ,  G01N33/521

    摘要： Dry transparent analytical elements can be used to determine analytes which have been separated by electrically induced migration through a solid medium, e.g. by electrophoresis, or which are intracellular enzymes. The dry transparent element is placed on a plate containing the analytes, and kept there until the analytes have reacted to produce a non-diffusible detectable species solely in the element. The element is removed and the detectable species is evaluated therein. The elements contain a water-insoluble binder material having an interactive composition dispersed therein which reacts with analyte to produce the non-diffusible species. The same electrophoretic plate can be used to successively determine the same or a plurality of analytes since it is not altered or destroyed by contact with the dry element.

    摘要翻译： 干透明分析元件可用于确定通过电致移动通过固体介质分离的分析物，例如， 通过电泳，或者是细胞内酶。 将干燥的透明元件放置在含有分析物的板上，并保持在那里，直到分析物已经反应，仅在该元素中产生不可扩散的可检测物质。 去除元素，并在其中评估可检测物质。 这些元素含有具有分散在其中的相互作用组合物的水不溶性粘合剂材料，其与分析物反应以产生不可扩散物质。 相同的电泳板可以用于连续地确定相同或多个分析物，因为它不会通过与干燥元件接触而改变或破坏。

    Dry analytical element for acetaminophen assay
    3.
    发明授权
    Dry analytical element for acetaminophen assay 失效
    对乙酰氨基酚测定用干燥分析元素

    公开(公告)号：US06783731B1

    公开(公告)日：2004-08-31

    申请号：US08493442

    申请日：1995-06-22

    申请人： Thomas C. Arter ,  John C. Mauck ,  James R. Schaeffer ,  Robert F. Winterkorn

    发明人： Thomas C. Arter ,  John C. Mauck ,  James R. Schaeffer ,  Robert F. Winterkorn

    IPC分类号： G01N2100

    CPC分类号： C12Q1/34 ,  G01N2333/98 ,  Y10S435/966 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/815

    摘要： A spectrophotometric assay for the detection of acetaminophen in aqueous fluids is carried out with a dry analytical element. The element comprises a support having thereon one or more reagent layers containing a first enzyme, aryl acylamidase, to cleave the amide bond of acetaminophen to produce p-aminophenol; and a mild oxidizing agent to oxidize the p-aminophenol so that it couples to a water-soluble coupling agent to form a dye that is read at 670 nm. The assay is precise, accurate on serum and plasma samples, and relatively free from significant interferences. The element also allows measurement over a broad dynamic range.

    摘要翻译： 用水分液体检测对乙酰氨基酚的分光光度法用干燥分析元件进行。 元件包括其上具有一个或多个试剂层的载体，其含有第一种酶，芳基酰胺酶，以切割对乙酰氨基酚的酰胺键以产生对氨基苯酚; 和一种温和的氧化剂，以氧化对氨基苯酚，使其与水溶性偶联剂偶联形成在670nm读取的染料。 该测定法在血清和血浆样品中精确，准确，并且相对没有显着的干扰。 该元件还允许在宽动态范围内进行测量。

    Analytical element and method for the determination of creatinine or creatine
    7.
    发明授权
    Analytical element and method for the determination of creatinine or creatine 失效
    用于测定肌酸酐或肌酸的分析元素和方法

    公开(公告)号：US4812399A

    公开(公告)日：1989-03-14

    申请号：US854460

    申请日：1986-04-21

    申请人： John C. Mauck ,  Linda A. Mauck ,  Gary E. Norton

    发明人： John C. Mauck ,  Linda A. Mauck ,  Gary E. Norton

    IPC分类号： C12Q1/26 ,  C12Q1/28 ,  C12Q1/34 ,  G01N33/70

    CPC分类号： C12Q1/34 ,  G01N33/70 ,  Y10S435/81

    摘要： An analytical element can be used for the determination of either creatinine or creatine or both. The element contains creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase, and a leuco dye which is capable of providing a detectable dye in the presence of hydrogen peroxide and a peroxidative substance. The creatine amidinohydrolase is present in a manner such that it is substantially inert to the leuco dye. The creatinine amidohydrolase is present in a rate limiting amount. By measuring the amount of dye formation at particular times during the assay, either or both of the analytes can be determined with the same element.

    摘要翻译： 分析元素可用于测定肌酐或肌酸或两者。 该元件含有肌酸酐酰胺水解酶，肌酸脒基水解酶和肌氨酸氧化酶，以及能够在过氧化氢和过氧化物质存在下提供可检测染料的无色染料。 肌酸脒基水解酶以使其对无色染料基本上是惰性的方式存在。 肌酐酰胺水解酶以限速量存在。 通过测量在测定期间特定时间的染料形成量，分析物中的一种或两种可以用相同的元素来测定。

    Analytical method and element for protein assay
    8.
    发明授权
    Analytical method and element for protein assay 失效
    蛋白质分析方法和元素

    公开(公告)号：US4786605A

    公开(公告)日：1988-11-22

    申请号：US64640

    申请日：1987-06-22

    申请人： John C. Mauck ,  Harold C. Warren, III

    发明人： John C. Mauck ,  Harold C. Warren, III

    IPC分类号： G01N33/52 ,  G01N33/68 ,  G01N33/00 ,  G01N21/00

    CPC分类号： G01N33/6833 ,  G01N33/526

    摘要： There is disclosed a method for quantitatively determining protein, comprising the steps of:(a) providing a sample of the protein in an aqueous medium;(b) providing an aqueous medium having a pH in excess of 12 and comprising(i) a cupric salt and a pyridyl-azo dye; or(ii) a preformed cupric-pyridylazo dye complex;(c) combining the aqueous mediums of a) and b) thereby providing a color having an intensity which is inversely proportional to the amount of unreacted dye present in the combined mediums; and(d) determining the quantity of protein present in the sample colorimetrically.The method can be used with a multilayer dry analytical element.