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公开(公告)号：US5980746A
公开(公告)日：1999-11-09
申请号：US966261
申请日：1997-11-07
IPC分类号： B01D67/00 , B01D71/20 , B01D71/26 , B01D71/56 , B01D71/68 , C08J5/18 , C08J9/36 , G01N33/544 , G01N33/545 , G01N33/548 , B01D39/18 , B01D71/10
CPC分类号： B01D67/0088 , B01D67/0018 , B01D71/20 , B01D71/68 , G01N33/544 , G01N33/545 , G01N33/548 , B01D2323/12 , B01D2325/02 , B01D2325/36 , Y10T428/249959
摘要： The present invention provides a membrane comprising (a) polymer solids comprising 60 wt. % or more of a fully synthetic organic polymer and optionally about 40 wt. % or less of a polymer of natural origin and (b) a cellulose compound which allows for the detection of a biological molecule of interest, wherein the cellulose compound is uniformly distributed throughout the surface of the membrane. The present invention also provides methods of preparing and using such membranes.
摘要翻译： 本发明提供一种膜，其包含（a）聚合物固体，其包含60wt。 完全合成的有机聚合物的％或更多，任选地约40wt。 ％以下的天然来源的聚合物和（b）允许检测感兴趣的生物分子的纤维素化合物，其中所述纤维素化合物均匀分布在膜的整个表面。 本发明还提供了制备和使用这种膜的方法。
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公开(公告)号：US07094347B2
公开(公告)日：2006-08-22
申请号：US11035950
申请日：2005-01-18
申请人： Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
发明人： Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
CPC分类号： B01D67/0088 , B01D15/361 , B01D15/363 , B01D61/00 , B01D61/14 , B01D69/02 , B01D69/10 , B01D69/125 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01D2325/16 , B01D2325/30 , B01D2325/36 , B01J20/28033 , B01J20/28035 , B01J20/3272 , B01J20/3282 , B01J41/12 , B01J41/13 , B01J41/20 , B01J47/12 , C12N15/1017 , G01N33/54393
摘要： The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.
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公开(公告)号：US20050139537A1
公开(公告)日：2005-06-30
申请号：US11037356
申请日：2005-01-19
申请人： Chung-Jen Hou , Peter Konstantin , Yujing Yang
发明人： Chung-Jen Hou , Peter Konstantin , Yujing Yang
IPC分类号： B01D15/36 , B01D61/00 , B01D61/14 , B01D67/00 , B01D71/08 , B01D71/40 , B01J20/28 , B01J20/32 , B01J39/20 , B01J39/26 , B01J47/12 , C07K1/34 , C12M1/34 , C02F1/44
CPC分类号： B01D67/0088 , B01D15/362 , B01D61/00 , B01D61/007 , B01D61/14 , B01D67/0009 , B01D71/08 , B01D71/40 , B01D2323/12 , B01D2323/30 , B01D2325/14 , B01D2325/36 , B01J20/261 , B01J20/262 , B01J20/264 , B01J20/267 , B01J20/28035 , B01J20/2804 , B01J20/28078 , B01J20/2808 , B01J20/286 , B01J20/3208 , B01J20/3212 , B01J20/327 , B01J20/3274 , B01J20/3282 , B01J39/20 , B01J39/26 , B01J47/12 , B01J2220/44 , B01J2220/54 , C07K1/34 , Y10T436/143333
摘要： The present invention provides, in certain embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating having fixed negative charges. The crosslinked coating can be prepared, e.g., from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- and/or N-(alkoxymethyl)-acrylamide, a hydrophilic unsaturated monomer, and an initiator. The present invention further provides, in some embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating prepared from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- or N-(alkoxymethyl)-acrylamide, a polysaccharide, and an initiator. The membranes of the present invention are suitable for use in ion exchange chromatography, for example, in the separation and purification of positively charged species such as proteins.
摘要翻译： 本发明在某些实施方案中提供了一种带负电荷的微多孔膜，其包含多孔基底和具有固定负电荷的交联涂层。 交联涂层可以例如由含有阴离子基团的不饱和单体，N-（羟甲基） - 和/或N-（烷氧基甲基） - 丙烯酰胺，亲水性不饱和单体和引发剂的聚合组合物制备。 在一些实施方案中，本发明进一步提供了一种带负电荷的微多孔膜，其包含多孔基材和由聚合组合物制备的交联涂层，所述聚合组合物包含具有阴离子基团的不饱和单体，N-（羟甲基） - 或N-（烷氧基甲基） - 丙烯酰胺，多糖和引发剂。 本发明的膜适用于离子交换色谱法，例如分离和纯化带正电荷的物质如蛋白质。
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公开(公告)号：US07223341B2
公开(公告)日：2007-05-29
申请号：US11480977
申请日：2006-07-06
申请人： Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
发明人： Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
CPC分类号： B01D67/0088 , B01D15/361 , B01D15/363 , B01D61/00 , B01D61/14 , B01D69/02 , B01D69/10 , B01D69/125 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01D2325/16 , B01D2325/30 , B01D2325/36 , B01J20/28033 , B01J20/28035 , B01J20/3272 , B01J20/3282 , B01J41/12 , B01J41/13 , B01J41/20 , B01J47/12 , C12N15/1017 , G01N33/54393
摘要： The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.
摘要翻译： 本发明提供了具有约25mg / ml或更大的蛋白结合能力的带正电荷的微多孔膜，其包含亲水性多孔基底和向膜提供固定的正电荷的交联涂层。 本发明还提供一种带正电的微多孔膜，其包括多孔基材和包含阳离子阳离子基团的交联涂层。 本发明的膜可用于各种应用，包括离子交换层析，大分子转移，以及生物分子如蛋白质，核酸，内毒素等的检测，过滤和纯化。
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公开(公告)号：US06851561B2
公开(公告)日：2005-02-08
申请号：US10891098
申请日：2004-07-15
申请人： Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
发明人： Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
IPC分类号： B01D15/36 , B01D61/00 , B01D61/14 , B01D63/14 , B01D67/00 , B01D69/02 , B01D69/10 , B01D69/12 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01J20/28 , B01J41/12 , B01J41/20 , B01J47/12 , B01D63/06 , B01D71/56
CPC分类号： B01D67/0088 , B01D15/361 , B01D15/363 , B01D61/00 , B01D61/14 , B01D69/02 , B01D69/10 , B01D69/125 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01D2325/16 , B01D2325/30 , B01D2325/36 , B01J20/28033 , B01J20/28035 , B01J20/3272 , B01J20/3282 , B01J41/12 , B01J41/13 , B01J41/20 , B01J47/12 , C12N15/1017 , G01N33/54393
摘要： The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.
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公开(公告)号：US07396465B2
公开(公告)日：2008-07-08
申请号：US11742749
申请日：2007-05-01
申请人： Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
发明人： Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
CPC分类号： B01D67/0088 , B01D15/361 , B01D15/363 , B01D61/00 , B01D61/14 , B01D69/02 , B01D69/10 , B01D69/125 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01D2325/16 , B01D2325/30 , B01D2325/36 , B01J20/28033 , B01J20/28035 , B01J20/3272 , B01J20/3282 , B01J41/12 , B01J41/13 , B01J41/20 , B01J47/12 , C12N15/1017 , G01N33/54393
摘要： The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.
摘要翻译： 本发明提供了具有约25mg / ml或更大的蛋白结合能力的带正电荷的微多孔膜，其包含亲水性多孔基底和向膜提供固定的正电荷的交联涂层。 本发明还提供一种带正电的微多孔膜，其包括多孔基材和包含阳离子阳离子基团的交联涂层。 本发明的膜可用于各种应用，包括离子交换层析，大分子转移，以及生物分子如蛋白质，核酸，内毒素等的检测，过滤和纯化。
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公开(公告)号：US07132049B2
公开(公告)日：2006-11-07
申请号：US11037356
申请日：2005-01-19
申请人： Chung-Jen Hou , Peter Konstantin , Yujing Yang
发明人： Chung-Jen Hou , Peter Konstantin , Yujing Yang
CPC分类号： B01D67/0088 , B01D15/362 , B01D61/00 , B01D61/007 , B01D61/14 , B01D67/0009 , B01D71/08 , B01D71/40 , B01D2323/12 , B01D2323/30 , B01D2325/14 , B01D2325/36 , B01J20/261 , B01J20/262 , B01J20/264 , B01J20/267 , B01J20/28035 , B01J20/2804 , B01J20/28078 , B01J20/2808 , B01J20/286 , B01J20/3208 , B01J20/3212 , B01J20/327 , B01J20/3274 , B01J20/3282 , B01J39/20 , B01J39/26 , B01J47/12 , B01J2220/44 , B01J2220/54 , C07K1/34 , Y10T436/143333
摘要： The present invention provides, in certain embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating having fixed negative charges. The crosslinked coating can be prepared, e.g., from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- and/or N-(alkoxymethyl)-acrylamide, a hydrophilic unsaturated monomer, and an initiator. The present invention further provides, in some embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating prepared from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- or N-(alkoxymethyl)-acrylamide, a polysaccharide, and an initiator. The membranes of the present invention are suitable for use in ion exchange chromatography, for example, in the separation and purification of positively charged species such as proteins.
摘要翻译： 本发明在某些实施方案中提供了一种带负电荷的微多孔膜，其包含多孔基底和具有固定负电荷的交联涂层。 交联涂层可以例如由含有阴离子基团的不饱和单体，N-（羟甲基） - 和/或N-（烷氧基甲基） - 丙烯酰胺，亲水性不饱和单体和引发剂的聚合组合物制备。 在一些实施方案中，本发明进一步提供了一种带负电荷的微多孔膜，其包含多孔基材和由聚合组合物制备的交联涂层，所述聚合组合物包含具有阴离子基团的不饱和单体，N-（羟甲基） - 或N-（烷氧基甲基） - 丙烯酰胺，多糖和引发剂。 本发明的膜适用于离子交换色谱法，例如分离和纯化带正电荷的物质如蛋白质。
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公开(公告)号：US20050211621A1
公开(公告)日：2005-09-29
申请号：US11035950
申请日：2005-01-18
申请人： Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
发明人： Xiaosong Wu , Chung-Jen Hou , Jayesh Dharia , Peter Konstantin , Yujing Yang
IPC分类号： B01D15/36 , B01D61/00 , B01D61/14 , B01D63/14 , B01D67/00 , B01D69/02 , B01D69/10 , B01D69/12 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01J20/28 , B01J41/12 , B01J41/20 , B01J47/12
CPC分类号： B01D67/0088 , B01D15/361 , B01D15/363 , B01D61/00 , B01D61/14 , B01D69/02 , B01D69/10 , B01D69/125 , B01D71/40 , B01D71/44 , B01D71/60 , B01D71/82 , B01D2325/16 , B01D2325/30 , B01D2325/36 , B01J20/28033 , B01J20/28035 , B01J20/3272 , B01J20/3282 , B01J41/12 , B01J41/13 , B01J41/20 , B01J47/12 , C12N15/1017 , G01N33/54393
摘要： The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.
摘要翻译： 本发明提供了具有约25mg / ml或更大的蛋白结合能力的带正电荷的微多孔膜，其包含亲水性多孔基底和向膜提供固定的正电荷的交联涂层。 本发明还提供一种带正电的微多孔膜，其包括多孔基材和包含阳离子阳离子基团的交联涂层。 本发明的膜可用于各种应用，包括离子交换层析，大分子转移，以及生物分子如蛋白质，核酸，内毒素等的检测，过滤和纯化。
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9.发明授权Filter device and filter element comprising at least one negatively charged membrane 有权过滤装置和过滤元件包括至少一个带负电荷的膜公开(公告)号：US06860393B2
公开(公告)日：2005-03-01
申请号：US10895048
申请日：2004-07-21
申请人： Chung-Jen Hou , Peter Konstantin , Yujing Yang
发明人： Chung-Jen Hou , Peter Konstantin , Yujing Yang
IPC分类号： B01D15/36 , B01D61/00 , B01D61/14 , B01D67/00 , B01D71/08 , B01D71/40 , B01J20/28 , B01J20/32 , B01J39/20 , B01J39/26 , B01J47/12 , C07K1/34 , B01D63/14 , B01D63/10 , B01D71/00
CPC分类号： B01D67/0088 , B01D15/362 , B01D61/00 , B01D61/007 , B01D61/14 , B01D67/0009 , B01D71/08 , B01D71/40 , B01D2323/12 , B01D2323/30 , B01D2325/14 , B01D2325/36 , B01J20/261 , B01J20/262 , B01J20/264 , B01J20/267 , B01J20/28035 , B01J20/2804 , B01J20/28078 , B01J20/2808 , B01J20/286 , B01J20/3208 , B01J20/3212 , B01J20/327 , B01J20/3274 , B01J20/3282 , B01J39/20 , B01J39/26 , B01J47/12 , B01J2220/44 , B01J2220/54 , C07K1/34 , Y10T436/143333
摘要： The present invention provides, in certain embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating having fixed negative charges. The crosslinked coating can be prepared, e.g., from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- and/or N-(alkoxymethyl)-acrylamide, a hydrophilic unsaturated monomer, and an initiator. The present invention further provides, in some embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating prepared from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- or N-(alkoxymethyl)-acrylamide, a polysaccharide, and an initiator. The membranes of the present invention are suitable for use in ion exchange chromatography, for example, in the separation and purification of positively charged species such as proteins.
摘要翻译： 本发明在某些实施方案中提供了一种带负电荷的微多孔膜，其包含多孔基底和具有固定负电荷的交联涂层。 交联涂层可以例如由含有阴离子基团的不饱和单体，N-（羟甲基） - 和/或N-（烷氧基甲基） - 丙烯酰胺，亲水性不饱和单体和引发剂的聚合组合物制备。 在一些实施方案中，本发明进一步提供了一种带负电荷的微多孔膜，其包含多孔基材和由聚合组合物制备的交联涂层，所述聚合组合物包含具有阴离子基团的不饱和单体，N-（羟甲基） - 或N-（烷氧基甲基） - 丙烯酰胺，多糖和引发剂。 本发明的膜适用于离子交换色谱法，例如分离和纯化带正电荷的物质如蛋白质。
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公开(公告)号：US06783937B1
公开(公告)日：2004-08-31
申请号：US09914165
申请日：2001-12-12
申请人： Chung-Jen Hou , Peter Konstantin , Yujing Yang
发明人： Chung-Jen Hou , Peter Konstantin , Yujing Yang
IPC分类号： C12Q168
CPC分类号： B01D67/0088 , B01D15/362 , B01D61/00 , B01D61/007 , B01D61/14 , B01D67/0009 , B01D71/08 , B01D71/40 , B01D2323/12 , B01D2323/30 , B01D2325/14 , B01D2325/36 , B01J20/261 , B01J20/262 , B01J20/264 , B01J20/267 , B01J20/28035 , B01J20/2804 , B01J20/28078 , B01J20/2808 , B01J20/286 , B01J20/3208 , B01J20/3212 , B01J20/327 , B01J20/3274 , B01J20/3282 , B01J39/20 , B01J39/26 , B01J47/12 , B01J2220/44 , B01J2220/54 , C07K1/34 , Y10T436/143333
摘要： The present invention provides, in certain embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating having fixed negative charges. The crosslinked coating can be prepared, e.g., from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- and/or N-(alkoxymethyl)-acrylamide, a hydrophilic unsaturated monomer, and an initiator. The present invention further provides, in some embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating prepared from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- or N-(alkoxymethyl)-acrylamide, a polysaccharide, and an initiator. The membranes of the present invention are suitable for use in ion exchange chromatography, for example, in the separation and purification of positively charged species such as proteins.
摘要翻译： 本发明在某些实施方案中提供了一种带负电荷的微多孔膜，其包含多孔基底和具有固定负电荷的交联涂层。 交联涂层可以例如由含有阴离子基团的不饱和单体，N-（羟甲基） - 和/或N-（烷氧基甲基） - 丙烯酰胺，亲水性不饱和单体和引发剂的聚合组合物制备。 在一些实施方案中，本发明进一步提供了一种带负电荷的微多孔膜，其包含多孔基材和由聚合组合物制备的交联涂层，所述聚合组合物包含具有阴离子基团的不饱和单体，N-（羟甲基） - 或N-（烷氧基甲基） - 丙烯酰胺，多糖和引发剂。 本发明的膜适用于离子交换色谱法，例如分离和纯化带正电荷的物质如蛋白质。
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