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公开(公告)号：US4506013A
公开(公告)日：1985-03-19
申请号：US275088
申请日：1981-06-18
IPC分类号： C12N1/21 , C07H21/04 , C12N1/00 , C12N1/06 , C12N15/00 , C12N15/09 , C12N15/63 , C12P19/34 , C12R1/01 , C12R1/125 , C12R1/19 , C12R1/38 , C12R1/425 , C12R1/44 , C12R1/46 , C12R1/465 , C12R1/645 , C12R1/66 , C12R1/745 , C12R1/80 , C12R1/91 , C12N1/20 , C12P21/00 , C12P21/02
CPC分类号： C12N15/635 , C12N1/06 , Y10S435/849
摘要： A method for stabilizing and selecting host cells containing recombinant DNA which expresses a functional polypeptide and the novel organisms and cloning vectors for the practice thereof. The invention further provides a simple, convenient, and inexpensive method to lyse host cells for purification of intracellular products.
摘要翻译： 用于稳定和选择含有表达功能性多肽的重组DNA的宿主细胞的方法，以及用于其实践的新生物和克隆载体。 本发明还提供了一种简单，方便和便宜的方法来裂解宿主细胞以纯化细胞内产物。
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2.发明授权Method for stabilizing and selecting recombinant DNA containing host cells 失效用于稳定和选择含有宿主细胞的重组DNA的方法
公开(公告)号：US4436815A
公开(公告)日：1984-03-13
申请号：US325511
申请日：1981-11-27
IPC分类号： C12N15/09 , C07H21/04 , C07K14/575 , C07K14/62 , C12N1/00 , C12N1/20 , C12N15/00 , C12N15/63 , C12N15/70 , C12P21/02 , C12R1/19 , C12P21/00
CPC分类号： C12N15/635 , C07K14/57581 , C07K14/62 , C12N15/70 , Y10S930/18
摘要： An improved method for stabilizing and selecting host cells containing recombinant DNA which expresses a functional polypeptide and the novel organisms and cloning vectors for the practice thereof.
摘要翻译： 一种用于稳定和选择含有表达功能性多肽的重组DNA的宿主细胞的改进方法，以及用于其实践的新生物和克隆载体。
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3.发明授权
公开(公告)号：US4650761A
公开(公告)日：1987-03-17
申请号：US550167
申请日：1983-11-09
IPC分类号： C07K14/575 , C07K14/62 , C12N15/63 , C12N15/70 , C12N15/00 , C07H21/04 , C12N1/00 , C12N1/20 , C12P19/34 , C12P21/00 , C12P21/02 , C12P21/04 , C12R1/19
CPC分类号： C12N15/635 , C07K14/57581 , C07K14/62 , C12N15/70 , Y10S435/849 , Y10S930/18
摘要： The present invention is an improvement in the method for stabilizing and selecting host cells containing recombinant DNA. The method involves transforming a host cell with a recombinant DNA cloning vector which contains the .about.2.5 kb BglII cI repressor gene-containing restriction fragment of bacteriophage .lambda. and then lysogenizing the transformed host cell with a lysogenic organism which contains a marker which is lethal or conditionally lethal in the host cell but which is repressed in the transformed host cell by the repressor gene contained in the recombinant DNA cloning vector. The vector additionally contains a gene which codes for the expression of human proinsulin. The invention further comprises related recombinant DNA cloning vectors, transformed host cells and lysogenized transformed host cells.
摘要翻译： 本发明是用于稳定和选择含有重组DNA的宿主细胞的方法的改进。 该方法包括用含有含噬菌体λ的DIFFERENCE 2.5kb BglII cI阻遏物基因限制性片段的重组DNA克隆载体转化宿主细胞，然后用含有致死或有条件的标记物的溶原性生物溶解转化的宿主细胞 在宿主细胞中致死，但是通过重组DNA克隆载体中包含的阻遏物基因在转化的宿主细胞中被抑制。 该载体还含有编码人胰岛素原表达的基因。 本发明还包括相关的重组DNA克隆载体，转化的宿主细胞和溶解的转化的宿主细胞。
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公开(公告)号：US4530904A
公开(公告)日：1985-07-23
申请号：US414290
申请日：1982-09-03
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N15/00 , C12P19/34 , C12P21/02 , C12R1/01 , C12R1/04 , C12R1/07 , C12R1/19 , C12R1/38 , C12R1/425 , C12R1/44 , C12R1/46 , C12R1/465 , C12N1/00 , C12P21/00 , C12P21/04
CPC分类号： C12P21/02 , C12N15/00 , Y10S435/849 , Y10S930/18
摘要： A novel method for protecting a bacterium from a naturally occurring bacteriophage and the cloning vectors and transformants for carrying out the aforementioned method are disclosed.
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公开(公告)号：US5576190A
公开(公告)日：1996-11-19
申请号：US156325
申请日：1993-11-23
申请人： Rama M. Belagaje , Charles L. Hershberger , Hansen M. Hsiung , Paul R. Rosteck, Jr. , Jane L. Sterner
发明人： Rama M. Belagaje , Charles L. Hershberger , Hansen M. Hsiung , Paul R. Rosteck, Jr. , Jane L. Sterner
IPC分类号： C12N1/21 , C07K14/61 , C12N15/00 , C12N15/09 , C12N15/11 , C12N15/73 , C12P21/02 , C12R1/19 , C12R1/92 , C12N15/67
摘要： The present invention concerns novel DNA compounds which function as transcriptional activating sequences. Recombinant DNA expression vectors which contain the transcriptional activating sequences and host cells transformed with these expression vectors are also provided. When properly positioned in an expression vector, the novel transcriptional activating sequences enable regulatable expression of an operably linked gene and enhance the structural stability of the expression vector.
摘要翻译： 本发明涉及用作转录激活序列的新型DNA化合物。 还提供了含有转录激活序列和用这些表达载体转化的宿主细胞的重组DNA表达载体。 当正确定位在表达载体中时，新的转录激活序列使得可操纵连接的基因的可调节表达增强表达载体的结构稳定性。
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公开(公告)号：US4732859A
公开(公告)日：1988-03-22
申请号：US647338
申请日：1984-09-05
CPC分类号： C12P21/02 , C12N15/00 , Y10S930/18
摘要： A novel method for protecting a bacterium from a naturally occurring bacteriophage and the cloning vectors and transformants for carrying out the aforementioned method are disclosed.
摘要翻译： 公开了一种用于保护细菌免受天然存在的噬菌体的新方法和用于实施上述方法的克隆载体和转化体。
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公开(公告)号：US5876991A
公开(公告)日：1999-03-02
申请号：US804227
申请日：1997-02-21
IPC分类号： C12N15/09 , C12N9/00 , C12N15/00 , C12N15/52 , C12P17/08 , C12P19/62 , C12R1/465 , C12R1/54 , C12N15/62 , C12N15/76
摘要： A DNA molecule isolated from Streptomyces fradiae encodes the multi-functional proteins which direct the synthesis of the polyketide tylactone.
摘要翻译： 从Streptomyces fradiae分离的DNA分子编码引导聚酮化物内酯的合成的多功能蛋白质。
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公开(公告)号：USH2085H1
公开(公告)日：2003-10-07
申请号：US09014897
申请日：1998-01-28
申请人： Jo Ann Hoskins , S. Richard Jaskunas, Jr. , Genshi Zhao , Pamela K. Rockey , Paul R. Rosteck, Jr. , Franklin H. Norris
发明人： Jo Ann Hoskins , S. Richard Jaskunas, Jr. , Genshi Zhao , Pamela K. Rockey , Paul R. Rosteck, Jr. , Franklin H. Norris
IPC分类号： C12N1509
摘要： The invention provides isolated nucleic acid compounds encoding a novel high molecular weight PBP of Streptococcus pneumoniae. Also provided are vectors and transformed heterologous host cells for expressing the PBP and a method for identifying compounds that bind and/or inhibit the enzymatic activity of the PBP.
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9.发明授权DNA encoding a novel penicillin binding protein from streptococcus pneumoniae 失效编码肺炎链球菌新型青霉素结合蛋白的DNA
公开(公告)号：US5789202A
公开(公告)日：1998-08-04
申请号：US731716
申请日：1996-10-17
申请人： Jo Ann Hoskins , S. Richard Jaskunas, Jr. , Pamela K. Rockey , Paul R. Rosteck, Jr. , Franklin H. Norris
发明人： Jo Ann Hoskins , S. Richard Jaskunas, Jr. , Pamela K. Rockey , Paul R. Rosteck, Jr. , Franklin H. Norris
IPC分类号： G01N33/50 , A61K38/00 , C07K14/315 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/31 , C12P21/02 , C12R1/46 , G01N33/15 , G01N33/566 , C07H21/04 , C12N15/00
CPC分类号： C07K14/3156 , A61K38/00
摘要： The invention provides isolated nucleic acid compounds encoding a novel high molecular weight PBP of Streptococcus pneumoniae. Also provided are vectors and transformed heterologous host cells for expressing the PBP and a method for identifying compounds that bind and/or inhibit the enzymatic activity of the PBP.
摘要翻译： 本发明提供编码肺炎链球菌新型高分子量PBP的分离的核酸化合物。 还提供了用于表达PBP的载体和转化的异源宿主细胞，以及用于鉴定结合和/或抑制PBP的酶活性的化合物的方法。
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公开(公告)号：US5945320A
公开(公告)日：1999-08-31
申请号：US804198
申请日：1997-02-21
申请人： Stanley G. Burgett , Stuart A. Kuhstoss , Ramachandra N. Rao , Mark A. Richardson , Paul R. Rosteck, Jr.
发明人： Stanley G. Burgett , Stuart A. Kuhstoss , Ramachandra N. Rao , Mark A. Richardson , Paul R. Rosteck, Jr.
IPC分类号： C12N15/09 , C07K14/195 , C07K14/41 , C12N9/00 , C12N15/52 , C12P17/08 , C12P19/62 , C12P21/02 , C12N15/63 , C12N15/76
摘要： A DNA molecule isolated from Streptomyces ambofaciens encodes the multi-functional proteins which direct the synthesis of the polyketide platenolide.
摘要翻译： 从链霉菌（Streptomyces ambofaciens）分离的DNA分子编码引导聚酮化合物底胶的合成的多功能蛋白质。
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