Process for producing trans-L-hydroxyproline
    2.
    发明授权
    Process for producing trans-L-hydroxyproline 失效
    生产反式-L-羟脯氨酸的方法

    公开(公告)号:US5364775A

    公开(公告)日:1994-11-15

    申请号:US972824

    申请日:1992-11-06

    摘要: The present invention provides a process for producing trans-L-hydroxyproline, which comprises culturing a microorganism which is capable of decomposing amino acids other than trans-L-hydroxyproline but which is substantially incapable of decomposing trans-L-hydroxyproline in a culture medium containing collagen hydrolyzate, and recovering trans-L-hydroxyproline from the resulting culture.The process enhances the content of trans-L-hydroxyproline based on the total weight of amino acids contained in collagen hydrolyzate, and enables efficient production of trans-L-hydroxyproline which is useful as a starting material for the synthesis of medicines.

    摘要翻译: 本发明提供了生产反式-L-羟基脯氨酸的方法,该方法包括培养能够分解反式-L-羟基脯氨酸以外的氨基酸但基本上不能分解反式-L-羟脯氨酸的微生物,该培养基含有 胶原水解产物,并从所得培养物中回收反式-L-羟脯氨酸。 该方法基于胶原蛋白水解产物中所含的氨基酸的总重量,提高了反式-L-羟脯氨酸的含量,能有效地制备可用作药物合成起始原料的反式-L-羟脯氨酸。

    Process for producing 4-hydroxy-L-proline
    3.
    发明授权
    Process for producing 4-hydroxy-L-proline 失效
    生产4-羟基-L-脯氨酸的方法

    公开(公告)号:US5374542A

    公开(公告)日:1994-12-20

    申请号:US39482

    申请日:1993-04-26

    CPC分类号: C12P13/24 C12N9/12

    摘要: Disclosed are a process for producing 4-hydroxy-L-proline by the conversion of 4-hydroxy-2-oxoglutaric acid in an aqueous medium in the presence of an amino group donor and an enzyme source belonging to the genus Escherichia and capable of converting 4-hydroxy-2-oxoglutaric acid into 4-hydroxy-L-proline; and a microorganism which belongs to the genus Escherichia, holds a recombinant DNA incorporating a gene coding for .gamma.-glutamylkinase released from the feedback inhibition caused by L-proline, and contains the above enzyme source.

    摘要翻译: PCT No.PCT / JP91 / 01127 Sec。 371日期:1993年4月26日 102(e)日期1993年4月26日PCT 1991年8月26日PCT公布。 公开号WO93 / 04181 日期:1993年3月4日。未公开的是通过在氨基供体和属于下列物质的酶源的情况下在水性介质中转化4-羟基-2-氧代戊二酸来生产4-羟基-L-脯氨酸的方法 能够将4-羟基-2-氧代戊二酸转化为4-羟基-L-脯氨酸; 属于埃希氏杆菌属的微生物,含有由L-脯氨酸引起的反馈抑制释放的编码γ-谷氨酰基激酶的基因的重组DNA,并含有上述酶源。

    Process for producing L-arginine
    4.
    发明授权
    Process for producing L-arginine 失效
    L-精氨酸生产方法

    公开(公告)号:US5017482A

    公开(公告)日:1991-05-21

    申请号:US99798

    申请日:1987-09-22

    摘要: L-arginine is produced by constructing a recombinant DNA composed of a vector DNA and a DNA fragment derived from chromosomal DNA of a microorganism belonging to the genus Corynebacterium or Brevibacterium and bearing genetic information relating to the synthesis of L-arginine-biosynthetic enzyme, introducing the recombinant DNA in a microorganism belonging to the genus Corynebacterium or Brevibacterium, culturing the microorganism in a medium, and recovering L-arginine accumulated in the culture broth.

    摘要翻译: 通过构建由载体DNA构成的重组DNA和衍生自属于棒状杆菌属或短杆菌属的微生物的染色体DNA的DNA片段,并具有与L-精氨酸 - 生物合成酶的合成有关的遗传信息,引入L-精氨酸,引入L-精氨酸 属于棒杆菌属或短杆菌属的微生物的重组DNA,在培养基中培养微生物,并回收在培养液中积累的L-精氨酸。

    Polymerase chain reaction amplification method using a single primer
which randomly anneals
    6.
    发明授权
    Polymerase chain reaction amplification method using a single primer which randomly anneals 失效
    聚合酶链反应扩增法使用单一引物随机退火

    公开(公告)号:US5665572A

    公开(公告)日:1997-09-09

    申请号:US294606

    申请日:1994-08-23

    CPC分类号: C12Q1/6832 C12Q1/686

    摘要: A method of amplifying template DNA by polymerase chain reaction (PCR) in which a single oligonucleotide primer having a restriction site is contacted with the template DNA, whereby the oligonucleotide randomly anneals to a single strand of the template DNA and DNA sequences complementary to the single strand are synthesized. An initial PCR amplification yields synthetic DNA sequences having the oligonucleotide sequence incorporated therein at the 5' end, and a sequence complementary to the template DNA. A second PCR amplification under higher stringency conditions amplifies regions of the template DNA to give DNA fragments having the restriction sites of the oligonucleotide primer. Thereby the method can be used to amplify trace quantities of template DNA of unknown sequence simply and efficiently, which has applications in the construction of DNA libraries of chromosome specific regions and the development of probes for chromosome mapping.

    摘要翻译: 通过聚合酶链式反应(PCR)扩增模板DNA的方法,其中将具有限制性位点的单个寡核苷酸引物与模板DNA接触,由此寡核苷酸随机退火到模板DNA的单链和与该单链互补的DNA序列 链合成。 初始PCR扩增产生在5'末端具有掺入寡核苷酸序列的合成DNA序列和与模板DNA互补的序列。 在较高严格条件下的第二次PCR扩增扩增模板DNA的区域,得到具有寡核苷酸引物限制性位点的DNA片段。 因此该方法可用于简单高效地扩增未知序列的痕量模板DNA,可用于构建染色体特异性区DNA文库,并开发用于染色体定位的探针。

    Desensitized aspartokinase
    7.
    发明授权
    Desensitized aspartokinase 有权
    脱敏天冬氨酸激酶

    公开(公告)号:US06893848B1

    公开(公告)日:2005-05-17

    申请号:US09958606

    申请日:2000-04-14

    CPC分类号: C12N9/1217 C12P13/08

    摘要: The present invention relates to a novel aspartokinase derived from a Coryneform bacterium; a DNA encoding the enzyme; a recombinant DNA containing the above DNA; a Coryneform bacterium having the above recombinant DNA or a Coryneform bacterium having the DNA on its chromosome; and a process for producing L-lysine by culturing the above microorganism. Construction has been successfully made of a DNA encoding an aspartokinase freed from concerted feedback inhibition by L-lysine and L-threonine derived from a Corynebacterium and has a nucleotide sequence encoding an amino acid sequence wherein the amino acid residue at position 311 is an amino acid other than Thr in the amino acid sequence shown by SEQ ID NO: 18.

    摘要翻译: 本发明涉及一种衍生自棒状杆菌属细菌的新型天冬氨酸激酶; 编码酶的DNA; 含有上述DNA的重组DNA; 具有上述重组DNA的棒状细菌或其染色体上具有DNA的棒状细菌; 以及通过培养上述微生物来生产L-赖氨酸的方法。 已经成功制备了编码天冬氨酸的DNA,其不含由L-赖氨酸和由棒状杆菌衍生的L-苏氨酸的协同反馈抑制,并且具有编码氨基酸序列的核苷酸序列,其中311位的氨基酸残基是氨基酸 SEQ ID NO:18所示的氨基酸序列中的Thr除外。

    Glucose-6-phosphate dehydrogenase
    10.
    发明授权
    Glucose-6-phosphate dehydrogenase 有权
    葡萄糖-6-磷酸脱氢酶

    公开(公告)号:US07078204B2

    公开(公告)日:2006-07-18

    申请号:US10312007

    申请日:2001-06-15

    摘要: The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as “G6PD”) derived from a bacterium belonging to the genus Corynebacterium, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant.According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.

    摘要翻译: 本发明涉及一种衍生自属于棒状杆菌属的细菌的新型葡萄糖-6-磷酸脱氢酶(以下称为“G6PD”),编码该酶的DNA,包含该DNA的重组DNA,包含重组体 DNA,包含其染色体上的DNA的转化体,以及生产L-氨基酸或G6PD的方法,其包括培养转化体。 根据本发明,获得了修饰的G6PD和编码G6PD的D​​NA,通过使用改良的G6PD可以提高微生物的L-氨基酸的生产率。