摘要:
Disclosed is a process for producing L-arginine by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of L-arginine and a vector DNA, culturing the transformant in a nutrient medium, accumulating L-arginine in the culture medium and recovering L-arginine therefrom.
摘要:
The present invention provides a process for producing trans-L-hydroxyproline, which comprises culturing a microorganism which is capable of decomposing amino acids other than trans-L-hydroxyproline but which is substantially incapable of decomposing trans-L-hydroxyproline in a culture medium containing collagen hydrolyzate, and recovering trans-L-hydroxyproline from the resulting culture.The process enhances the content of trans-L-hydroxyproline based on the total weight of amino acids contained in collagen hydrolyzate, and enables efficient production of trans-L-hydroxyproline which is useful as a starting material for the synthesis of medicines.
摘要:
Disclosed are a process for producing 4-hydroxy-L-proline by the conversion of 4-hydroxy-2-oxoglutaric acid in an aqueous medium in the presence of an amino group donor and an enzyme source belonging to the genus Escherichia and capable of converting 4-hydroxy-2-oxoglutaric acid into 4-hydroxy-L-proline; and a microorganism which belongs to the genus Escherichia, holds a recombinant DNA incorporating a gene coding for .gamma.-glutamylkinase released from the feedback inhibition caused by L-proline, and contains the above enzyme source.
摘要:
L-arginine is produced by constructing a recombinant DNA composed of a vector DNA and a DNA fragment derived from chromosomal DNA of a microorganism belonging to the genus Corynebacterium or Brevibacterium and bearing genetic information relating to the synthesis of L-arginine-biosynthetic enzyme, introducing the recombinant DNA in a microorganism belonging to the genus Corynebacterium or Brevibacterium, culturing the microorganism in a medium, and recovering L-arginine accumulated in the culture broth.
摘要:
Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer, and use of them.
摘要:
A method of amplifying template DNA by polymerase chain reaction (PCR) in which a single oligonucleotide primer having a restriction site is contacted with the template DNA, whereby the oligonucleotide randomly anneals to a single strand of the template DNA and DNA sequences complementary to the single strand are synthesized. An initial PCR amplification yields synthetic DNA sequences having the oligonucleotide sequence incorporated therein at the 5' end, and a sequence complementary to the template DNA. A second PCR amplification under higher stringency conditions amplifies regions of the template DNA to give DNA fragments having the restriction sites of the oligonucleotide primer. Thereby the method can be used to amplify trace quantities of template DNA of unknown sequence simply and efficiently, which has applications in the construction of DNA libraries of chromosome specific regions and the development of probes for chromosome mapping.
摘要:
The present invention relates to a novel aspartokinase derived from a Coryneform bacterium; a DNA encoding the enzyme; a recombinant DNA containing the above DNA; a Coryneform bacterium having the above recombinant DNA or a Coryneform bacterium having the DNA on its chromosome; and a process for producing L-lysine by culturing the above microorganism. Construction has been successfully made of a DNA encoding an aspartokinase freed from concerted feedback inhibition by L-lysine and L-threonine derived from a Corynebacterium and has a nucleotide sequence encoding an amino acid sequence wherein the amino acid residue at position 311 is an amino acid other than Thr in the amino acid sequence shown by SEQ ID NO: 18.
摘要翻译:本发明涉及一种衍生自棒状杆菌属细菌的新型天冬氨酸激酶; 编码酶的DNA; 含有上述DNA的重组DNA; 具有上述重组DNA的棒状细菌或其染色体上具有DNA的棒状细菌; 以及通过培养上述微生物来生产L-赖氨酸的方法。 已经成功制备了编码天冬氨酸的DNA,其不含由L-赖氨酸和由棒状杆菌衍生的L-苏氨酸的协同反馈抑制,并且具有编码氨基酸序列的核苷酸序列,其中311位的氨基酸残基是氨基酸 SEQ ID NO:18所示的氨基酸序列中的Thr除外。
摘要:
A new strain Lactobacillus sp. KPB-176, which does not possess strict selectivity for specific media and which involves no reduction in the productivity of polysaccharides even during subculture, was isolated from kefir grains. When this new strain is cultured on a medium containing milk whey and casamino acid or when on a medium containing carbohydrate and yeast extract, capsular polysaccharides are produced in high yields.
摘要:
Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer, and use of them.
摘要:
The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as “G6PD”) derived from a bacterium belonging to the genus Corynebacterium, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant.According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.