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公开(公告)号:US20110139274A1
公开(公告)日:2011-06-16
申请号:US13030379
申请日:2011-02-18
CPC分类号: G01N27/44743 , B01L3/5027 , B01L3/502715 , B01L3/502753 , B01L9/527 , B01L2200/027 , B01L2200/0636 , B01L2300/0816 , B01L2300/0887 , B01L2300/1894 , B01L2400/0421 , B01L2400/0481 , G01N27/44717 , G01N27/44791 , G01N30/84 , G01N33/5438 , Y10T29/49826 , Y10T137/8158 , Y10T137/8376
摘要: The invention provides fluidic devices having incorporated electrodes, systems for using such devices, and methods for manufacturing such devices. The invention also provides fluidic devices configured to absorb joule heating within the device.
摘要翻译: 本发明提供了具有并入电极的流体装置,用于使用这种装置的系统以及用于制造这种装置的方法。 本发明还提供了被配置为吸收装置内的焦耳加热的流体装置。
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公开(公告)号:US08367021B2
公开(公告)日:2013-02-05
申请号:US13030379
申请日:2011-02-18
CPC分类号: G01N27/44743 , B01L3/5027 , B01L3/502715 , B01L3/502753 , B01L9/527 , B01L2200/027 , B01L2200/0636 , B01L2300/0816 , B01L2300/0887 , B01L2300/1894 , B01L2400/0421 , B01L2400/0481 , G01N27/44717 , G01N27/44791 , G01N30/84 , G01N33/5438 , Y10T29/49826 , Y10T137/8158 , Y10T137/8376
摘要: The invention provides fluidic devices having incorporated electrodes. One device comprises a card and a caddy. The card includes a channel disposed within the card, first and second vias in fluid communication with the channel through an upper surface of the card, and first and second electrodes disposed on the upper surface of the card. The first via and first electrode are positioned adjacent to a first end of the channel, and the second via and second electrode are positioned adjacent to a second end of the channel. The caddy comprises first and second reservoirs. The caddy is attached to the card such that the first reservoir is positioned over the first via and a portion of the first electrode, and the second reservoir is positioned over the second via and a portion of the second electrode. A portion of each of the electrodes is accessible for dry electrical contact.
摘要翻译: 本发明提供具有并入电极的流体装置。 一个设备包括一个卡和一个球童。 卡片包括布置在卡内的通道,通过卡的上表面与通道流体连通的第一和第二通孔以及设置在卡的上表面上的第一和第二电极。 第一通孔和第一电极定位成与通道的第一端相邻,并且第二通孔和第二电极定位成与通道的第二端相邻。 球童包括第一和第二储层。 所述卡盘连接到所述卡片上,使得所述第一储存器位于所述第一通孔上方,并且所述第一电极的一部分位于所述第二通孔和所述第二电极的一部分上。 每个电极的一部分可用于干电接触。
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公开(公告)号:US08545771B2
公开(公告)日:2013-10-01
申请号:US13290843
申请日:2011-11-07
IPC分类号: G01N27/447 , G01N27/453
CPC分类号: G01N27/44791 , B01L3/5027 , B01L2200/0636 , B01L2200/0689 , B01L2300/021 , B01L2300/0645 , B01L2300/0861 , B01L2400/0421 , G01N27/44717
摘要: The invention provides fluidic devices having incorporated electrodes. One device comprises a card and first and second caddy segments. The first caddy segment comprises first and second electrodes. The second caddy segment comprises first and second reservoirs disposed on a first surface of the second segment, a channel disposed on a second surface of the second segment, and first and second vias extending between the first and second surfaces. The first caddy segment is attached to the first surface of the second caddy segment. The card is attached to the second surface of the second caddy segment such that the card provides a closed surface for the device.
摘要翻译: 本发明提供具有并入电极的流体装置。 一个设备包括卡和第一和第二球童段。 第一个扇区段包括第一和第二电极。 第二球笼段包括设置在第二段的第一表面上的第一和第二储存器,设置在第二段的第二表面上的通道以及在第一和第二表面之间延伸的第一和第二通孔。 第一个球童段连接到第二个球童段的第一个表面。 该卡被附接到第二球童段的第二表面,使得该卡为该装置提供一个封闭的表面。
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公开(公告)号:US20120080315A1
公开(公告)日:2012-04-05
申请号:US13290843
申请日:2011-11-07
IPC分类号: G01N27/447 , G01N27/453
CPC分类号: G01N27/44791 , B01L3/5027 , B01L2200/0636 , B01L2200/0689 , B01L2300/021 , B01L2300/0645 , B01L2300/0861 , B01L2400/0421 , G01N27/44717
摘要: The invention provides fluidic devices having incorporated electrodes, systems for using such devices, and methods for manufacturing such devices. The invention also provides fluidic devices configured to absorb joule heating within the device.
摘要翻译: 本发明提供了具有并入电极的流体装置,用于使用这种装置的系统以及用于制造这种装置的方法。 本发明还提供了被配置为吸收装置内的焦耳加热的流体装置。
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公开(公告)号:US08871524B2
公开(公告)日:2014-10-28
申请号:US13287052
申请日:2011-11-01
申请人: Dan Camporese , Advit Bhatt , Josh Molho , Hui Xu , Ken Summers
发明人: Dan Camporese , Advit Bhatt , Josh Molho , Hui Xu , Ken Summers
IPC分类号: G01N15/06 , G01N33/00 , G01N33/48 , B01L3/00 , G01N27/447
CPC分类号: G01N27/44717 , B01L3/502738 , B01L3/502753 , B01L2200/0636 , B01L2300/021 , B01L2400/0421 , B01L2400/0655 , G01N27/44778 , G01N27/44791
摘要: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.
摘要翻译: 本发明提供了进行尺寸分析的方法。 在这些方法中,用于执行尺寸分析的尺寸梯度被校正。 在一种方法中,对筛分凝胶中的批次间变化校正施胶梯。 在另一种方法中,针对与归档梯级浓度不同的样品浓度校正施胶梯。 还提供了一种方法,其中使用样品中的标准标记和/或使用实时标准施胶梯来校正施胶梯。 所述方法可以单独使用或组合使用。
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公开(公告)号:US09034196B2
公开(公告)日:2015-05-19
申请号:US13238342
申请日:2011-09-21
申请人: Stephane Mouradian , Josh Molho , Ming Wu , Hayley Wu , Javier A. Farinas
发明人: Stephane Mouradian , Josh Molho , Ming Wu , Hayley Wu , Javier A. Farinas
CPC分类号: B01L3/502761 , B01L3/502715 , B01L2200/027 , B01L2300/0681 , B01L2300/0816 , B01L2300/0819
摘要: A microfluidic device with a filter includes a substrate; a flowpath including a well formed in the substrate in fluid communication with a channel formed in the substrate; and a filter disposed across the flowpath and associated with the channel.
摘要翻译: 具有过滤器的微流体装置包括:基板; 流路,其包括在所述基板中形成的与形成在所述基板中的通道流体连通的阱; 以及跨过流路设置并与通道相关联的过滤器。
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公开(公告)号:US08277744B2
公开(公告)日:2012-10-02
申请号:US13471300
申请日:2012-05-14
申请人: Josh Molho , Hui Xu , Jorge J. Zaninovich
发明人: Josh Molho , Hui Xu , Jorge J. Zaninovich
CPC分类号: B01L3/502776 , B01L3/502738 , B01L3/502753 , B01L2200/0636 , B01L2400/0421 , G01N27/44717 , G01N27/44791 , G01N30/84 , Y10T137/0318 , Y10T137/877 , Y10T137/87788
摘要: The invention provides a device, system, and method for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. The device includes first and second pinching channels, a separation channel extending between the first and second pinching channels, a collection leg that includes a collection well between first and second ends of the collection leg, and a waste leg, all of which are in fluid communication with a switching region. In the method, a sample material is separated into a plurality of separated components in the device and one or more of the separated components are isolated in the collection well. The separated components are constrained and elongated in the switching region by first and second buffer streams.
摘要翻译: 本发明提供一种用于在将样品材料分离成多个样品组分之后分离样品材料的一个或多个样品组分的装置,系统和方法。 该装置包括第一和第二夹持通道,在第一和第二夹持通道之间延伸的分离通道,收集腿,其包括在收集腿的第一和第二端之间的收集井,以及废物腿,所有这些都处于流体 与切换区域的通信。 在该方法中,将样品材料分离成装置中的多个分离的组分,并将一个或多个分离的组分隔离在收集阱中。 分离的组件在开关区域中受到第一和第二缓冲流的限制和拉长。
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公开(公告)号:US20080277356A1
公开(公告)日:2008-11-13
申请号:US11745411
申请日:2007-05-07
申请人: Stephane Mouradian , Josh Molho , Ming Wu , Hayley Wu , Javier A. Farinas
发明人: Stephane Mouradian , Josh Molho , Ming Wu , Hayley Wu , Javier A. Farinas
CPC分类号: B01L3/502761 , B01L3/502715 , B01L2200/027 , B01L2300/0681 , B01L2300/0816 , B01L2300/0819
摘要: A microfluidic device with a filter includes a substrate; a flowpath including a well formed in the substrate in fluid communication with a channel formed in the substrate; and a filter disposed across the flowpath.
摘要翻译: 具有过滤器的微流体装置包括基板; 流路,其包括在所述基板中形成的与形成在所述基板中的通道流体连通的阱; 以及设置在流路上的过滤器。
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公开(公告)号:US09539574B2
公开(公告)日:2017-01-10
申请号:US12902006
申请日:2010-10-11
申请人: Charles Park , Persefoni Kechagia , Michael Spaid , Morten Jensen , Irina G. Kazakova , Josh Molho
发明人: Charles Park , Persefoni Kechagia , Michael Spaid , Morten Jensen , Irina G. Kazakova , Josh Molho
IPC分类号: G01N27/447 , B01L3/00
CPC分类号: B01L3/502753 , B01L3/502715 , B01L3/502746 , B01L2200/0673 , B01L2200/16 , B01L2300/0816 , B01L2400/0415 , B01L2400/084 , G01N27/44743 , G01N27/44773 , G01N27/44791
摘要: This invention provides methods and systems for injection of analytes into a separation channel for resolution and detection. Samples can be preconditioned and concentrated by isotachophoresis (ITP) before the injection is triggered by a detected voltage event. Separation of analytes from other sample constituents can be enhanced using skewing channel ITP.
摘要翻译: 本发明提供了用于将分析物注入到用于分辨和检测的分离通道中的方法和系统。 样品可以通过等速电泳(ITP)进行预处理和浓缩,然后通过检测到的电压事件触发注射。 使用偏斜通道ITP可以提高分析物与其他样品成分的分离。
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公开(公告)号:US08834807B2
公开(公告)日:2014-09-16
申请号:US13287071
申请日:2011-11-01
申请人: Josh Molho , Hui Xu
发明人: Josh Molho , Hui Xu
CPC分类号: G01N27/44791 , B01L3/502738 , B01L3/502753 , B01L2200/0636 , B01L2300/0816 , B01L2400/0421 , B01L2400/0655 , G01N27/44717 , Y10T137/0391 , Y10T137/877
摘要: The invention provides devices and methods for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. A device includes a separation channel having a sample loading well. A low-conductivity buffer is disposed in the loading well, the buffer having a conductivity
摘要翻译: 本发明提供了用于在将样品材料分离成多个样品组分之后隔离样品材料的一个或多个样品组分的装置和方法。 一种装置包括具有样品加载井的分离通道。 低电导率缓冲液设置在负载阱中,缓冲器具有<0.2mS / cm的电导率。 在一种方法中,将缓冲器加载到与装置的分离通道流体连通的装载井中。 然后将具有高于缓冲器的导电率的样品材料加载到装载井中,使得样品材料设置在缓冲液下方,缓冲液设置在样品材料上并覆盖其上。 样品材料在分离通道中分离成多个分离的组分,并且从设置在装置的收集腿中的收集井收集分离的组分。
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