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1.发明授权
公开(公告)号：US5620850A
公开(公告)日：1997-04-15
申请号：US312388
申请日：1994-09-26
IPC分类号： C12Q1/68 , G01N33/543 , C07K2/00 , C07K5/00 , G01N33/53
CPC分类号： B82Y30/00 , B82Y15/00 , B82Y40/00 , C07H21/04 , C12Q1/6837 , G01N33/54353 , G01N33/54373 , G01N33/553 , G01N2610/00
摘要： An article suitable for use as a biosensor includes a species of a formula X--R--Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
摘要翻译： 适合用作生物传感器的制品包括作为自组装单层的一部分粘附到制品表面的式X-R-Ch的种类。 X是附着于表面的官能团，R是间隔基，Ch是螯合剂。 金属离子可以由螯合剂配位，并且靶向生物分子的聚氨基酸标记的生物结合配偶体与金属离子配位。 本发明的方法包括使制品与含有或怀疑含有靶生物分子的培养基接触并允许生物分子与结合配偶体生物结合。 该文章特别用作表面等离子体共振芯片。
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2.发明授权公开(公告)号：US06809196B2
公开(公告)日：2004-10-26
申请号：US09915187
申请日：2001-07-25
IPC分类号： C07H2104
CPC分类号： B82Y30/00 , B82Y15/00 , B82Y40/00 , C07H21/04 , C12Q1/6837 , G01N33/54353 , G01N33/54373 , G01N33/553 , G01N2610/00
摘要： An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
摘要翻译： 适合用作生物传感器的制品包括作为自组装单层的一部分粘附到制品表面的式X-R-Ch分子。 X是附着于表面的官能团，R是间隔基，Ch是螯合剂。 金属离子可以由螯合剂配位，并且靶向生物分子的聚氨基酸标记的生物结合配偶体与金属离子配位。 本发明的方法包括使制品与含有或怀疑含有靶生物分子的培养基接触并允许生物分子与结合配偶体生物结合。 该文章特别用作表面等离子体共振芯片。
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公开(公告)号：US06472148B1
公开(公告)日：2002-10-29
申请号：US08786187
申请日：1997-01-21
IPC分类号： C12Q168
CPC分类号： C12Q1/6837 , B82Y15/00 , B82Y30/00 , B82Y40/00 , G01N33/54373 , G01N2610/00
摘要： An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
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公开(公告)号：US06322979B1
公开(公告)日：2001-11-27
申请号：US09296078
申请日：1999-04-21
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B82Y15/00 , B82Y40/00 , C07H21/04 , C12Q1/6837 , G01N33/54353 , G01N33/54373 , G01N33/553 , G01N2610/00
摘要： An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
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5.发明授权公开(公告)号：US06197515B1
公开(公告)日：2001-03-06
申请号：US09296111
申请日：1999-04-21
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B82Y15/00 , B82Y40/00 , C07H21/04 , C12Q1/6837 , G01N33/54353 , G01N33/54373 , G01N33/553 , G01N2610/00
摘要： An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
摘要翻译： 适合用作生物传感器的制品包括作为自组装单层的一部分粘附到制品表面的式X-R-Ch分子。 X是附着于表面的官能团，R是间隔基，Ch是螯合剂。 金属离子可以由螯合剂配位，并且靶向生物分子的聚氨基酸标记的生物结合配偶体与金属离子配位。 本发明的方法包括使制品与含有或怀疑含有靶生物分子的培养基接触并允许生物分子与结合配偶体生物结合。 该文章特别用作表面等离子体共振芯片。
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公开(公告)号：US09464642B2
公开(公告)日：2016-10-11
申请号：US13885967
申请日：2011-11-21
申请人： Filip Ilievski , Xin Chen , Aaron D. Mazzeo , George M. Whitesides , Robert F. Shepherd , Ramses V. Martinez , Won Jae Choi , Sen W. Kwok , Stephen Morin , Adam Stokes , Zhihong Nie
发明人： Filip Ilievski , Xin Chen , Aaron D. Mazzeo , George M. Whitesides , Robert F. Shepherd , Ramses V. Martinez , Won Jae Choi , Sen W. Kwok , Stephen Morin , Adam Stokes , Zhihong Nie
CPC分类号： F15B15/10 , B25J9/1075 , B25J9/142 , B25J11/00 , F15B7/06
摘要： A soft robotic device includes a flexible body having a width, a length and a thickness, wherein the thickness is at least 1 mm, the flexible body having at least one channel disposed within the flexible body, the channel defined by upper, lower and side walls, wherein at least one wall is strain limiting; and a pressurizing inlet in fluid communication with the at least one channel, the at least one channel positioned and arranged such that the wall opposite the strain limiting wall preferentially expands when the soft robotic device is pressurized through the inlet.
摘要翻译： 软机器人装置包括具有宽度，长度和厚度的柔性体，其中所述厚度为至少1mm，所述柔性体具有设置在所述柔性体内的至少一个通道，所述通道由上，下和侧限定 壁，其中至少一个壁是应变限制; 以及与所述至少一个通道流体连通的加压入口，所述至少一个通道定位和布置成使得当所述软自动装置通过所述入口被加压时，与所述应变限制壁相对的壁优先地膨胀。
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7.发明授权公开(公告)号：US09409265B2
公开(公告)日：2016-08-09
申请号：US13994595
申请日：2011-12-20
申请人： George M. Whitesides , Filip Ilievski , Audrey Ellerbee , Sergey S. Shevkoplyas , Katherine A. Mirica
发明人： George M. Whitesides , Filip Ilievski , Audrey Ellerbee , Sergey S. Shevkoplyas , Katherine A. Mirica
CPC分类号： B23P19/04 , B03C1/01 , B03C1/288 , B03C2201/18 , B81C3/005 , B81C2203/054 , B81C2203/057 , B81C2203/058 , C30B30/04 , Y10S117/901 , Y10T29/49826
摘要： The ability to assemble three-dimensional structures using diamagnetic particles suspended in solutions containing paramagnetic cations is described. The major advantages of this separation device are that: (i) it is a simple apparatus that does not require electric power (a set of permanent magnets and gravity are sufficient for the diamagnetic separation and collection system to work); ii) the assembled structures can be removed from the paramagnetic solution for further processing after fixing the structure; iii) the assembly is fast; and iv) it is small, portable.
摘要翻译： 描述了使用悬浮在含有顺磁阳离子的溶液中的反磁性颗粒组装三维结构的能力。 这种分离装置的主要优点是：（i）它是一种不需要电力的简单装置（一组永磁体和重力足以使反磁性分离和收集系统工作）; ii）组装的结构可以在固定结构之后从顺磁溶液中除去以进一步处理; iii）装配快速; 和iv）它是小，便携式。
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公开(公告)号：US08985547B2
公开(公告)日：2015-03-24
申请号：US11883260
申请日：2006-01-31
申请人： Douglas B. Weibel , Andrew Lee , Scott Potenta , Adam Siegel , Maarten Kruithof , George M. Whitesides
发明人： Douglas B. Weibel , Andrew Lee , Scott Potenta , Adam Siegel , Maarten Kruithof , George M. Whitesides
CPC分类号： F15B1/04 , B01L3/502738 , B01L3/502746 , B01L2200/0636 , B01L2300/0867 , B01L2400/0487 , B01L2400/0655 , B01L2400/082 , F15B2201/21 , F15B2201/305 , F15B2201/4053 , F16K99/0001 , F16K99/0026 , F16K99/0034 , F16K2099/0074 , F16K2099/0078 , F16K2099/0084 , Y10T137/0318 , Y10T137/0396 , Y10T137/2213 , Y10T137/4824 , Y10T137/6161
摘要： A microfluidic valve assembly includes a structure defining a microfluidic fluid path and an actuator that can be moved between different positions controlling flow through the channel. In one embodiment, the actuator can be threaded into at least a portion of the structure, and can be moved rotationally between a first position, causing relatively greater constriction of a microfluidic fluid path, and a second position causing relatively lesser constriction of the fluid path. Actuating the actuator, e.g., by rotation, can deform material between the valve and the fluid path, thereby constricting at least a portion of the underlying fluid path and regulating the flow of a fluid in the fluid path. In another aspect, the invention provides a reservoir into which fluid can be placed and from which fluid can be introduced into a microfluidic system. In one embodiment, the reservoir is expandable and thereby able to store fluid under pressure for delivery to a microfluidic system.
摘要翻译： 微流体阀组件包括限定微流体流体路径的结构和可以在控制流过通道的不同位置之间移动的致动器。 在一个实施例中，致动器可以被螺纹连接到结构的至少一部分中，并且可以在第一位置和第二位置之间旋转地移动，第一位置引起微流体流体路径的相对较大的缩小，并且引起相对较小的流体路径收缩的第二位置 。 例如通过旋转来致动致动器可以在阀和流体路径之间变形材料，从而收缩下面的流体路径的至少一部分并且调节流体路径中的流体的流动。 在另一方面，本发明提供了一种储存器，流体可以放置在该储存器中，并且可以从该储存器将流体引入到微流体系统中。 在一个实施例中，储存器是可膨胀的并且因此能够在压力下存储流体以递送到微流体系统。
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公开(公告)号：US20140206559A1
公开(公告)日：2014-07-24
申请号：US14119479
申请日：2012-05-23
IPC分类号： G01N33/483
CPC分类号： G01N33/4833 , G01N33/5091 , G01N33/574
摘要： The present invention relates to methods, compositions and kits related to a novel in vitro assay for a high-capacity and high-throughput method for measuring the ability of cancer cells to migrate in a three-dimensional cellular assay. The three-dimensional cellular invasion assay provides a method for determining and quantitating the metastatic potential and invasive capacity of a cancer cell. Other aspects of the invention further relate to the use of the in vitro assay to screen for agents and compounds capable of inhibiting intravasation, and thereby modulating the metastatic potential of cancer cells. The methods, compositions and three-dimensional assay provide a highly sensitive assay system capable of mimicking the in vivo cellular and molecular interactions required for successful completion of intravasation.
摘要翻译： 本发明涉及与用于测量癌细胞在三维细胞测定中迁移的能力的高容量和高通量方法的新体外测定相关的方法，组合物和试剂盒。 三维细胞侵袭测定提供了确定和定量癌细胞转移潜能和侵袭能力的方法。 本发明的其它方面进一步涉及使用体外测定来筛选能够抑制渗透的药剂和化合物，从而调节癌细胞的转移潜力。 方法，组合物和三维测定提供了能够模拟成功完成渗透所需的体内细胞和分子相互作用的高度灵敏的测定系统。
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公开(公告)号：US08635749B2
公开(公告)日：2014-01-28
申请号：US12844314
申请日：2010-07-27
申请人： Brian Mayers , Sandip Agarwal , Jeffrey Carbeck , David Ledoux , Kevin Randall Stewart , George M. Whitesides , Adam Winkleman
发明人： Brian Mayers , Sandip Agarwal , Jeffrey Carbeck , David Ledoux , Kevin Randall Stewart , George M. Whitesides , Adam Winkleman
IPC分类号： A44B18/00
CPC分类号： C09J7/00 , C09J2201/626
摘要： Disclosed are adhesive systems and methods of making and using such systems. Exemplary adhesive systems include protrusions and/or grooves that can interleave to form a reversible adhesive interaction.
摘要翻译： 公开了制造和使用这种系统的粘合剂系统和方法。 示例性的粘合剂系统包括可以交织以形成可逆粘合剂相互作用的突起和/或凹槽。
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