公开(公告)号：US6136552A

公开(公告)日：2000-10-24

申请号：US956507

申请日：1997-10-23

申请人： Daniel F. Klessig ,  He Du

发明人： Daniel F. Klessig ,  He Du

IPC分类号： C07K14/415 ,  G01N33/53

CPC分类号： C07K14/415

摘要： A high-affinity salicylic acid-binding protein (SABP2) derivable from tobacco and Arabidopsis is disclosed. The tobacco protein has a molecular weight of approximately 25 kDa and reversibly binds SA with an apparent K.sub.d of approximately 90 nM and a B.sub.max of 10 fmol/mg protein. The SABP2 of the invention may be used to identify analogues of SA. Analogues so identified may be used in plants to augment disease-resistance response pathways or other SA-sensitive processes in which SA plays a role. Possible examples include flowering and alternative respiration. The SABP2 of the invention may also be used to identify and clone a gene or cDNA that encodes it, which then may be used to generate transgenic plants having altered SABP2 levels.

摘要翻译： 公开了一种可从烟草和拟南芥衍生的高亲和力水杨酸结合蛋白（SABP2）。 烟草蛋白的分子量约为25kDa，可逆地结合SA，表观Kd为约90nM，Bmax为10fmol / mg蛋白。 本发明的SABP2可以用于鉴定SA的类似物。 如此鉴定的类似物可用于植物中以增强抗病反应途径或其中SA起作用的其他SA敏感过程。 可能的例子包括开花和替代呼吸。 本发明的SABP2还可以用于鉴定和克隆编码它的基因或cDNA，然后可以将其用于产生具有改变的SABP2水平的转基因植物。

公开(公告)号：US5646029A

公开(公告)日：1997-07-08

申请号：US276452

申请日：1994-07-18

申请人： Chao-Guang Chen ,  Shaio-Lim Mau ,  He Du ,  Alison M. Gane ,  Antony Bacic ,  Adrienne E. Clarke

发明人： Chao-Guang Chen ,  Shaio-Lim Mau ,  He Du ,  Alison M. Gane ,  Antony Bacic ,  Adrienne E. Clarke

IPC分类号： C12N15/09 ,  A23J1/00 ,  A61K38/00 ,  A61K47/48 ,  C07H21/04 ,  C07K14/415 ,  C07K16/16 ,  C12N5/10 ,  C12N15/82 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/91 ,  C12N1/21 ,  C12N5/00 ,  C12N5/06 ,  C12N5/14

CPC分类号： C07K14/415 ,  A23J1/007 ,  C12N15/8241 ,  A61K38/00

摘要： This invention provides plant arabinogalactan proteins (AGPs) and their genes. AGPs were isolated from Nicotiana alata, Nicotiana plumbaginafolia, and Pyrus communis. Amino acid sequences of isolated AGP peptide fragments are presented. Isolated AGP fragments were used to synthesize oligonucleotide probes to prepare oligonucleotide primers for PCR or prepare RNA probes to screen cDNA libraries of N. alata, N. plumbaginafolia, and P. communis. cDNA clones encoding amino acid sequences of isolated AGP fragments were isolated. The invention presents for the first time an intact AGP amino acid sequence derived from a corresponding AGP gene. The instant invention further provides methods useful in obtaining AGP genes encoding an AGP peptide comprising a specific isolated hydroxyproline-rich (OAST-rich) sequence or a specific isolated hydroxyproline-poor sequence.

摘要翻译： 本发明提供植物阿拉伯半乳聚糖蛋白（AGP）及其基因。 从Nicotiana alata，Nicotiana plumbaginafolia和Pyrus communis分离AGPs。 提出了分离的AGP肽片段的氨基酸序列。 使用分离的AGP片段合成寡核苷酸探针以制备用于PCR的寡核苷酸引物或制备RNA探针以筛选N.alata，N.plumbaginafolia和P. communis的cDNA文库。 分离编码分离的AGP片段的氨基酸序列的cDNA克隆。 本发明首次呈现源自相应AGP基因的完整AGP氨基酸序列。 本发明还提供了可用于获得编码AGP肽的AGP基因的方法，所述AGP肽包含特异性分离的富含脯氨酸（富含OAST）（OAST）序列或特异性分离的不存在羟脯氨酸的序列。

公开(公告)号：US5638030A

公开(公告)日：1997-06-10

申请号：US655984

申请日：1996-05-31

申请人： He Du

发明人： He Du

IPC分类号： H03K3/012 ,  H03K3/0231 ,  H03K3/03 ,  H03B5/24 ,  H03L7/099

CPC分类号： H03K3/03 ,  H03K3/012 ,  H03K3/0231 ,  H03K3/0322

摘要： A VCO includes a ring oscillator formed by connecting a plurality of voltage controlled inverting delay cells together, a biasing circuit for providing a bias voltage to each of the voltage controlled inverting delay cells, and a source-follower transistor for providing a control voltage to the biasing circuit and voltage controlled inverting delay cells. Each of the voltage controlled inverting delay cells includes a first and a second plurality of transistors which define two outputs of the voltage controlled inverting delay cell, and a clipper transistor connected between the two outputs to short them together whenever a difference between a bias voltage provided to a gate of the clipper transistor by the biasing circuit and a voltage on either one of the two outputs exceeds a threshold voltage of the clipper transistor. The biasing circuit includes a third plurality of transistors which are matched with corresponding ones of the first and second pluralities of transistors and the clipper transistor such that the bias voltage generated by the biasing circuit automatically changes so as to be substantially equal to voltages corresponding to a HIGH logic state on the two outputs of each voltage controlled inverting delay cell as the control voltage provided to the biasing circuit and each of the voltage controlled inverting delay cells changes.

摘要翻译： VCO包括通过将多个电压控制的反相延迟单元连接在一起形成的环形振荡器，用于向每个电压控制的反相延迟单元提供偏置电压的偏置电路，以及用于向控制电压提供控制电压的源极跟随器晶体管 偏置电路和电压控制反相延迟单元。 每个电压控制的反相延迟单元包括限定电压控制的反相延迟单元的两个输出的第一和第二多个晶体管，以及连接在两个输出之间的限幅晶体管，以便每当所提供的偏置电压之间的差 通过偏置电路到限幅晶体管的栅极，并且两个输出中的任一个上的电压超过限幅晶体管的阈值电压。 偏置电路包括与第一和第二多个晶体管中的相应晶体管和限幅晶体管相匹配的第三多个晶体管，使得由偏置电路产生的偏置电压自动改变，以便基本上等于对应于 每个电压控制的反相延迟单元的两个输出上的高逻辑状态作为提供给偏置电路的控制电压和每个电压控制反相延迟单元发生变化。

公开(公告)号：US5830747A

公开(公告)日：1998-11-03

申请号：US798744

申请日：1997-02-13

申请人： Chao-Guang Chen ,  Shaio-Lim Mau ,  He Du ,  Alison M. Gane ,  Antony Bacic ,  Adrienne E. Clarke

发明人： Chao-Guang Chen ,  Shaio-Lim Mau ,  He Du ,  Alison M. Gane ,  Antony Bacic ,  Adrienne E. Clarke

IPC分类号： C12N15/09 ,  A23J1/00 ,  A61K38/00 ,  A61K47/48 ,  C07H21/04 ,  C07K14/415 ,  C07K16/16 ,  C12N5/10 ,  C12N15/82 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/91 ,  C12N1/20 ,  A01H1/04 ,  C12N15/00

CPC分类号： C07K14/415 ,  A23J1/007 ,  C12N15/8241 ,  A61K38/00

摘要： This invention provides plant arabinogalactan proteins (AGPs) and their genes. AGPs were isolated from Nicotiana alata, Nicotiana plumbaginafolia, and Pyrus communis. Amino acid sequences of isolated AGP peptide fragments are presented. Isolated AGP fragments were used to synthesize oligonucleotide probes to prepare oligonucleotide primers for PCR or prepare RNA probes to screen cDNA libraries of N. alata, N. plumbaginafolia, and P. communis. cDNA clones encoding amino acid sequences of isolated AGP fragments were isolated. The invention presents for the first time an intact AGP amino acid sequence derived from a corresponding AGP gene. The instant invention further provides methods useful in obtaining AGP genes encoding an AGP peptide comprising a specific isolated hydroxyproline-rich (OAST-rich) sequence or a specific isolated hydroxyproline-poor sequence.

摘要翻译： 本发明提供植物阿拉伯半乳聚糖蛋白（AGP）及其基因。 从Nicotiana alata，Nicotiana plumbaginafolia和Pyrus communis分离AGPs。 提出了分离的AGP肽片段的氨基酸序列。 使用分离的AGP片段合成寡核苷酸探针以制备用于PCR的寡核苷酸引物或制备RNA探针以筛选N.alata，N.plumbaginafolia和P. communis的cDNA文库。 分离编码分离的AGP片段的氨基酸序列的cDNA克隆。 本发明首次呈现源自相应AGP基因的完整AGP氨基酸序列。 本发明还提供了可用于获得编码AGP肽的AGP基因的方法，所述AGP肽包含特异性分离的富含脯氨酸（富含OAST）（OAST）序列或特异性分离的不存在羟脯氨酸的序列。

公开(公告)号：US5568099A

公开(公告)日：1996-10-22

申请号：US534538

申请日：1995-09-27

申请人： He Du

发明人： He Du

IPC分类号： H03K3/012 ,  H03K3/0231 ,  H03K3/03 ,  H03B5/24 ,  H03L7/099

CPC分类号： H03K3/03 ,  H03K3/012 ,  H03K3/0231 ,  H03K3/0322

摘要： A VCO includes a ring oscillator formed by connecting a plurality of voltage controlled inverting delay cells together, a biasing circuit for providing a bias voltage to each of the voltage controlled inverting delay cells, and a source-follower transistor for providing a control voltage to the biasing circuit and voltage controlled inverting delay cells. Each of the voltage controlled inverting delay cells includes a first and a second plurality of transistors which define two outputs of the voltage controlled inverting delay cell, and a clipper transistor connected between the two outputs to short them together whenever a difference between a bias voltage provided to a gate of the clipper transistor by the biasing circuit and a voltage on either one of the two outputs exceeds a threshold voltage of the clipper transistor. The biasing circuit includes a third plurality of transistors which are matched with corresponding ones of the first and second pluralities of transistors and the clipper transistor such that the bias voltage generated by the biasing circuit automatically changes so as to be substantially equal to voltages corresponding to a HIGH logic state on the two outputs of each voltage controlled inverting delay cell as the control voltage provided to the biasing circuit and each of the voltage controlled inverting delay cells changes.

摘要翻译： VCO包括通过将多个电压控制的反相延迟单元连接在一起形成的环形振荡器，用于向每个电压控制的反相延迟单元提供偏置电压的偏置电路，以及用于向控制电压提供控制电压的源极跟随器晶体管 偏置电路和电压控制反相延迟单元。 每个电压控制的反相延迟单元包括限定电压控制的反相延迟单元的两个输出的第一和第二多个晶体管，以及连接在两个输出之间的限幅晶体管，以便每当所提供的偏置电压之间的差 通过偏置电路到限幅晶体管的栅极，并且两个输出中的任一个上的电压超过限幅晶体管的阈值电压。 偏置电路包括与第一和第二多个晶体管中的相应晶体管和限幅晶体管相匹配的第三多个晶体管，使得由偏置电路产生的偏置电压自动改变，以便基本上等于对应于 每个电压控制的反相延迟单元的两个输出上的高逻辑状态作为提供给偏置电路的控制电压和每个电压控制反相延迟单元发生变化。

公开(公告)号：US5559476A

公开(公告)日：1996-09-24

申请号：US455311

申请日：1995-05-31

申请人： Zhongxuan Zhang ,  He Du

发明人： Zhongxuan Zhang ,  He Du

IPC分类号： H03K3/354 ,  H03B5/20 ,  H03K3/013 ,  H03K3/0231 ,  H03K3/03 ,  H03K5/13 ,  H03L7/089 ,  H03L7/099 ,  H03B5/02

CPC分类号： H03K3/013 ,  H03K3/0231 ,  H03K3/03 ,  H03K3/0322 ,  H03K5/131 ,  H03K5/133 ,  H03L7/0891 ,  H03L7/0995

摘要： A VCO includes a ring oscillator formed by connecting a plurality of voltage controlled inverting delay cells together, and a plurality of transistors for providing control voltages to the plurality of voltage controlled inverting delay cells. Preferably, each transistor has a drain connected to a reference voltage, and a source connected to a voltage controlled inverting delay cell paired to that transistor. Consequently, each transistor acts as a source-follower so that it provides a control voltage to its corresponding voltage controlled inverting delay cell which follows a control voltage driving its gate, thereby isolating the control voltage provided to its corresponding voltage controlled inverting delay cell from power supply noise.

摘要翻译： VCO包括通过将多个电压控制的反相延迟单元连接在一起形成的环形振荡器和用于向多个电压控制的反相延迟单元提供控制电压的多个晶体管。 优选地，每个晶体管具有连接到参考电压的漏极，以及连接到与该晶体管成对的电压控制反相延迟单元的源极。 因此，每个晶体管起到源极跟随器的作用，从而为其对应的受电压控制的反相延迟单元提供控制电压，该反相延迟单元遵循驱动其栅极的控制电压，从而将提供给其相应的电压控制反相延迟单元的控制电压与功率隔离 供应噪音。

公开(公告)号：US5502680A

公开(公告)日：1996-03-26

申请号：US389293

申请日：1995-02-16

申请人： He Du ,  Yun-Ti Wang

发明人： He Du ,  Yun-Ti Wang

IPC分类号： G11C11/419 ,  G11C7/06 ,  H03K19/096 ,  G11C11/40

CPC分类号： G11C7/065 ,  G11C7/062

摘要： A sense amplifier circuit includes a differential input circuit which receives first and second data inputs, din1and din2, and generates, in response to a first control signal .PHI..sub.1 being active LOW, a differential voltage across first and second nodes, which is indicative of a voltage difference between the first and second data inputs, din1 and din2; a pull-up circuit which connects, in response to a second control signal .PHI..sub.2 being active LOW, a high voltage reference Vdd to both the first and second nodes; a latching circuit which generates and latches, in response to voltages provided on the first and second nodes by the differential input and pull-up circuits, first and second latched data outputs; and an equalization circuit which equalizes, in response to a third control signal .PHI..sub.0 being active LOW, voltages on data lines respectively connected to the first and second data outputs. Timing of the first and second control signals, .PHI..sub.1 and .PHI..sub.2, is such that the second control signal .PHI..sub.2 is activated LOW after a finite period following the initial activation of the first control signal .PHI..sub.1 . The third control signal .PHI..sub.0 is preferably activated LOW when the first and second control signals, .PHI..sub.1 and .PHI..sub.2, are inactive HIGH.

摘要翻译： 读出放大器电路包括差分输入电路，其接收第一和第二数据输入din1和din2，并且响应于第一控制信号PHI1为低电平而产生跨越第一和第二节点的差分电压，其表示 第一和第二数据输入之间的电压差din1和din2; 上拉电路，响应于第二控制信号PHI 2为有效LOW，将高电压参考值Vdd连接到第一和第二节点; 锁存电路，响应于由差分输入和上拉电路在第一和第二节点上提供的电压而产生和锁存第一和第二锁存数据输出; 以及均衡电路，其响应于第三控制信号PHI 0为低电平而均衡，分别连接到第一和第二数据输出的数据线上的电压。 第一和第二控制信号PHI 1和PHI 2的定时使得第二控制信号PHI 2在第一控制信号PHI 1的初始激活之后的有限周期之后被激活为低电平。 当第一和第二控制信号PHI 1和PHI 2处于非活动状态时，第三控制信号PHI 0优选地被激活为低电平。