摘要:
A system and method for efficiently evaluating and executing unresolved data variables used as input for functional processes in a previously defined acyclic dataflow graph (or sequence of instructions which may be so represented). Embodiments of the present invention also contemplate maintaining previously computed values of data elements so that re-evaluation of the entire data flow graph or portions thereof may not be necessary.
摘要:
Method and apparatus for use in a digital data processing system that evaluates functional networks. A method operates to assign a unique signature to each input constant of the network, assign a unique signature to each output of a network Function, and to store each of the constants and function outputs along with their assigned signatures. The signatures operate as an access key to the associated stored constants and function outputs, thereby eliminating a requirement to reevaluate a Function if its outputs already exist. A storage element, or Dictionary (20), stores data units, such as input data, intermediate values, and/or pointers to same, each with a unique signature. The storage element, in concert with a Dictionary Controller (18), provides for the deletion of a stored data unit that is associated with a specified signature and the retrieval of a stored data unit that is associated with a specified signature. Preferably, the signatures are generated from a combination of constant and Function attributes and values and are each uniquely encoded by a CRC generator (14).
摘要:
The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
摘要:
The present invention is directed generally to metal binding compounds which may be added to cell culture media to replace factors required for cultivation of the cells (e.g. transferrin) which are of animal or human origin. More specifically, the invention is directed to metal binding compounds or complexes thereof comprising one or more transition element cations (such as ferrous or ferric ions), which are added to cell and tissue culture medium compositions. The metal binding compounds may be added to the media alone or may be first complexed with a transition metal ion. The invention is also directed to methods of use of such compositions, including, for example, methods for the cultivation of eukaryotic cells, particularly animal cells, in vitro. The invention also relates to compositions comprising such culture media and one or more cells, and to kits comprising one or more of the above-described compositions. The compositions of the present invention obviate the need for naturally derived metal-binding proteins, such as transferrin and ceruloplasmin, which may contain blood-borne pathogens.
摘要:
A uniform mechanism for building containment hierarchies separates the code/method which actually perform the containment insertion operations on the components from the specification of the containment and then provides an automatic way of obtaining and executing that code when containment hierarchies need to be created. The mechanism consists of an abstract syntax for describing the containment relationships, processing mechanism for creating the containment mechanism, and a registry containing the mechanisms for implementing specific containment policies. Having this abstraction allows an application programmer to be able to construct hierarchies of objects without having to be concerned about the specific details of how that containment is performed. In fact, the details may be deferred until run-time (and even downloaded from elsewhere). Also, having this abstraction significantly simplifies automatic generation of object hierarchy codes, both from the point of view its implementation as well as that of the code that is generated.
摘要:
A generalized mechanism manages ad-hoc type conversions in computer software. A management framework improves type conversion tasks by enabling the modularization of this task by partitioning the work of defining an individual type convertor from the task of identifying and locating type convertors on demand. The details of how type convertors are applied on demand are also a function of the management framework.
摘要:
The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
摘要:
A method and apparatus aids consistent, high-quality input of meta-information associated with items inserted into a database by coupling a hierarchical subject taxonomy, used to definitively assign an element, with sets of attributes appropriate for each category. Each attribute in turn is itself associated with a set of legal values drawn from a universe of appropriately typed values. The method and apparatus can be used to enable a user of a database management system to input or augment a set of semantically relevant and consistent meta-information associated with content in or being placed into the database management system. The content in the database system is placed into one or more of a set of hierarchical taxonomic categories. Zero or more semantically relevant attributes are associated with each taxonomic category. Relevant sets of values for each attribute, drawn from a universe of appropriate values, are associated with each attribute at each level in the taxonomic hierarchy. The placement of an element into a category adds the hierarchical set of attributes associated with that category to those relevant to the element. The value sets associated with those attributes may then be used to select consistent and appropriate meta-information to be associated with the element.
摘要:
Certain embodiments of the disclosed technology may include systems and apparatus for extracting and delivering nutrients from biomass for plant growth. In an example implementation, an apparatus is provided for supporting a multi-level system for extracting and distributing nutrients from biomass for plant growth, the apparatus includes two or more support structures. Each support structure includes two or more vertical strength members disposed substantially vertically on opposing sides of a corrugated base cylinder and are stabilized by at least one corrugations associated with the base cylinder. The apparatus includes two or more horizontal retaining members in communication with the respective two or more vertical strength members and operable to secure the two or more vertical strength members against the base cylinder. The apparatus also includes one or more upper troughs supported by the two or more support structures and disposed in a substantially horizontal position.
摘要:
The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.