-
公开(公告)号：US06446083B1
公开(公告)日：2002-09-03
申请号：US09570569
申请日：2000-05-12
IPC分类号： G06F1730
CPC分类号： G06F17/30011 , Y10S707/99933 , Y10S707/99935 , Y10S707/99943 , Y10S707/99945 , Y10S707/99948
摘要： A method and apparatus aids consistent, high-quality input of meta-information associated with items inserted into a database by coupling a hierarchical subject taxonomy, used to definitively assign an element, with sets of attributes appropriate for each category. Each attribute in turn is itself associated with a set of legal values drawn from a universe of appropriately typed values. The method and apparatus can be used to enable a user of a database management system to input or augment a set of semantically relevant and consistent meta-information associated with content in or being placed into the database management system. The content in the database system is placed into one or more of a set of hierarchical taxonomic categories. Zero or more semantically relevant attributes are associated with each taxonomic category. Relevant sets of values for each attribute, drawn from a universe of appropriate values, are associated with each attribute at each level in the taxonomic hierarchy. The placement of an element into a category adds the hierarchical set of attributes associated with that category to those relevant to the element. The value sets associated with those attributes may then be used to select consistent and appropriate meta-information to be associated with the element.
摘要翻译： 一种方法和装置通过将用于明确地分配元素的分级主题分类与适合于每个类别的属性集合相耦合来辅助与插入到数据库中的项目相关联的元信息的一致的，高质量的输入。 每个属性本身又与一系列从适当类型值的宇宙中绘制的合法值相关联。 该方法和装置可以用于使数据库管理系统的用户能够输入或增加与数据库管理系统中的内容相关联的一组语义相关和一致的元信息。 数据库系统中的内容被放置在一组分层分类学类别中的一个或多个中。 零个或多个语义相关的属性与每个分类学类别相关联。 从适当值的一个宇宙绘制的每个属性的相关值集与分类层次结构中的每个级别的每个属性相关联。 将元素放置到类别中将与该类别相关联的属性的分层集合添加到与元素相关的属性。 然后可以使用与这些属性相关联的值集合来选择与元素相关联的一致和适当的元信息。
-
公开(公告)号：US08455246B2
公开(公告)日：2013-06-04
申请号：US12463351
申请日：2009-05-08
申请人： Stephen Gorfien , Richard Fike , Glenn Godwin , Joyce Dzimian , David A. Epstein , Dale Gruber , Paul Price
发明人： Stephen Gorfien , Richard Fike , Glenn Godwin , Joyce Dzimian , David A. Epstein , Dale Gruber , Paul Price
IPC分类号： A61K33/32 , A61K33/34 , A01N59/16 , C12N5/00 , C12N5/02 , C12N5/07 , C12N5/10 , C12N11/00 , C12N11/16
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
摘要翻译： 本发明提供了支持哺乳动物细胞，特别是上皮细胞和成纤维细胞的体外培养，特别是悬浮液的细胞培养基制剂，以及使用这些培养基在体外悬浮培养哺乳动物细胞的方法。 培养基包含基础培养基和聚阴离子或聚阴离子化合物，优选多磺化或多硫酸化合物，更优选硫酸葡聚糖。 本发明还提供化学上定义的，无蛋白质的真核细胞培养基，其包含铁螯合物和锌，其能够支持悬浮培养物中的生长（特别是哺乳动物细胞的高密度生长），从而增加表达水平 的培养细胞中的重组蛋白，和/或增加培养细胞中的病毒产生。
-
3.发明申请METAL BINDING COMPOUNDS AND THEIR USE IN CELL CULTURE MEDIUM COMPOSITIONS 审中-公开金属结合化合物及其在细胞培养基组合物中的应用公开(公告)号：US20110183375A1
公开(公告)日：2011-07-28
申请号：US13013629
申请日：2011-01-25
CPC分类号： C12N5/0037 , A61K38/00 , A61K39/00 , C12N2500/24 , C12N2500/34
摘要： The present invention is directed generally to metal binding compounds which may be added to cell culture media to replace factors required for cultivation of the cells (e.g. transferrin) which are of animal or human origin. More specifically, the invention is directed to metal binding compounds or complexes thereof comprising one or more transition element cations (such as ferrous or ferric ions), which are added to cell and tissue culture medium compositions. The metal binding compounds may be added to the media alone or may be first complexed with a transition metal ion. The invention is also directed to methods of use of such compositions, including, for example, methods for the cultivation of eukaryotic cells, particularly animal cells, in vitro. The invention also relates to compositions comprising such culture media and one or more cells, and to kits comprising one or more of the above-described compositions. The compositions of the present invention obviate the need for naturally derived metal-binding proteins, such as transferrin and ceruloplasmin, which may contain blood-borne pathogens.
摘要翻译： 本发明一般涉及金属结合化合物，其可加入到细胞培养基中以替代用于培养动物或人来源的细胞（例如转铁蛋白）所需的因子。 更具体地，本发明涉及包含一种或多种过渡元素阳离子（例如亚铁或三价铁离子）的金属结合化合物或其复合物，其被添加到细胞和组织培养基组合物中。 金属结合化合物可以单独加入到介质中，或者可以首先与过渡金属离子络合。 本发明还涉及这种组合物的使用方法，包括例如在体外培养真核细胞，特别是动物细胞的方法。 本发明还涉及包含这种培养基和一种或多种细胞的组合物，以及包含一种或多种上述组合物的试剂盒。 本发明的组合物不需要可能含有血源性病原体的天然衍生的金属结合蛋白，例如转铁蛋白和血浆铜蓝蛋白。
-
公开(公告)号：US06536033B1
公开(公告)日：2003-03-18
申请号：US09238430
申请日：1999-01-28
IPC分类号： G06F945
CPC分类号： G06F9/4488
摘要： A uniform mechanism for building containment hierarchies separates the code/method which actually perform the containment insertion operations on the components from the specification of the containment and then provides an automatic way of obtaining and executing that code when containment hierarchies need to be created. The mechanism consists of an abstract syntax for describing the containment relationships, processing mechanism for creating the containment mechanism, and a registry containing the mechanisms for implementing specific containment policies. Having this abstraction allows an application programmer to be able to construct hierarchies of objects without having to be concerned about the specific details of how that containment is performed. In fact, the details may be deferred until run-time (and even downloaded from elsewhere). Also, having this abstraction significantly simplifies automatic generation of object hierarchy codes, both from the point of view its implementation as well as that of the code that is generated.
摘要翻译： 用于构建包容层次的统一机制将实际对包含的规范的组件进行封闭插入操作的代码/方法分离，然后提供在需要创建包含层次结构时获取和执行该代码的自动方式。 该机制包括用于描述包容关系的抽象语法，用于创建遏制机制的处理机制，以及包含用于实施特定遏制策略的机制的注册表。 具有这种抽象允许应用程序员能够构建对象的层次结构，而不必关心如何执行该控制的具体细节。 事实上，细节可能会推迟到运行时（甚至从其他地方下载）。 此外，具有这种抽象大大简化了对象层次结构代码的自动生成，从其实现的角度以及生成的代码的角度来看都是如此。
-
公开(公告)号：US06408299B1
公开(公告)日：2002-06-18
申请号：US09238431
申请日：1999-01-28
IPC分类号： G06F700
CPC分类号： G06F17/30952 , G06F9/465 , Y10S707/99942 , Y10S707/99945
摘要： A generalized mechanism manages ad-hoc type conversions in computer software. A management framework improves type conversion tasks by enabling the modularization of this task by partitioning the work of defining an individual type convertor from the task of identifying and locating type convertors on demand. The details of how type convertors are applied on demand are also a function of the management framework.
摘要翻译： 广义机制管理计算机软件中的特殊类型转换。 管理框架通过使从任务识别和定位类型转换器的任务划分工作来实现对任务的模块化，从而改进了类型转换任务。 类型转换器如何应用的细节也是管理框架的一个功能。
-
公开(公告)号：US5423040A
公开(公告)日：1995-06-06
申请号：US181457
申请日：1994-01-14
CPC分类号： G06F8/311
摘要： A system and method for efficiently evaluating and executing unresolved data variables used as input for functional processes in a previously defined acyclic dataflow graph (or sequence of instructions which may be so represented). Embodiments of the present invention also contemplate maintaining previously computed values of data elements so that re-evaluation of the entire data flow graph or portions thereof may not be necessary.
摘要翻译： 用于有效地评估和执行未被解析的数据变量的系统和方法，所述数据变量用作先前定义的非循环数据流图（或可能如此表示的指令序列）中的功能过程的输入。 本发明的实施例还考虑维护先前计算的数据元素值，使得整个数据流图或其部分的重新评估可能不是必需的。
-
公开(公告)号：US20090280533A1
公开(公告)日：2009-11-12
申请号：US12463371
申请日：2009-05-08
申请人： Stephen F. Gorfien , Richard M. Fike , Glenn P. Godwin , Joyce L. Dzimian , David A. Epstein , Dale Gruber , Don McClure , Paul J. Price
发明人： Stephen F. Gorfien , Richard M. Fike , Glenn P. Godwin , Joyce L. Dzimian , David A. Epstein , Dale Gruber , Don McClure , Paul J. Price
IPC分类号： C12P21/00
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
摘要翻译： 本发明提供了支持哺乳动物细胞，特别是上皮细胞和成纤维细胞的体外培养，特别是悬浮液的细胞培养基制剂，以及使用这些培养基在体外悬浮培养哺乳动物细胞的方法。 培养基包含基础培养基和聚阴离子或聚阴离子化合物，优选多磺化或多硫酸化合物，更优选硫酸葡聚糖。 本发明还提供化学上定义的，无蛋白质的真核细胞培养基，其包含铁螯合物和锌，其能够支持悬浮培养物中的生长（特别是哺乳动物细胞的高密度生长），从而增加表达水平 的培养细胞中的重组蛋白，和/或增加培养细胞中的病毒产生。
-
8.发明授权Systems and apparatus for extracting and delivering nutrients from biomass for plant growth 有权从生物量提取和提供营养物质用于植物生长的系统和设备公开(公告)号：US09426949B1
公开(公告)日：2016-08-30
申请号：US13680073
申请日：2012-11-18
CPC分类号： A01G31/06 , A01G9/023 , A01K63/003 , A47G7/02 , Y02P60/216
摘要： Certain embodiments of the disclosed technology may include systems and apparatus for extracting and delivering nutrients from biomass for plant growth. In an example implementation, an apparatus is provided for supporting a multi-level system for extracting and distributing nutrients from biomass for plant growth, the apparatus includes two or more support structures. Each support structure includes two or more vertical strength members disposed substantially vertically on opposing sides of a corrugated base cylinder and are stabilized by at least one corrugations associated with the base cylinder. The apparatus includes two or more horizontal retaining members in communication with the respective two or more vertical strength members and operable to secure the two or more vertical strength members against the base cylinder. The apparatus also includes one or more upper troughs supported by the two or more support structures and disposed in a substantially horizontal position.
摘要翻译： 所公开技术的某些实施方案可以包括用于从用于植物生长的生物质中提取和递送营养物的系统和装置。 在一个示例性实施方式中，提供了一种用于支持用于从用于植物生长的生物质中提取和分配营养物的多级系统的装置，该装置包括两个或更多个支撑结构。 每个支撑结构包括两个或更多个垂直强度构件，其基本垂直地布置在波纹基座圆筒的相对侧上，并且通过与基座圆筒相关联的至少一个波纹来稳定。 该装置包括与相应的两个或更多个垂直强度构件连通的两个或更多个水平保持构件，并且可操作以将两个或更多个垂直强度构件固定抵靠基座筒。 该装置还包括由两个或更多个支撑结构支撑并且设置在基本上水平的位置的一个或多个上槽。
-
公开(公告)号：US08785194B2
公开(公告)日：2014-07-22
申请号：US12463371
申请日：2009-05-08
申请人： Stephen Gorfien , Richard Fike , Glenn Godwin , Joyce Dzimian , David A. Epstein , Dale Gruber , Don McClure , Paul Price
发明人： Stephen Gorfien , Richard Fike , Glenn Godwin , Joyce Dzimian , David A. Epstein , Dale Gruber , Don McClure , Paul Price
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
摘要翻译： 本发明提供了支持哺乳动物细胞，特别是上皮细胞和成纤维细胞的体外培养，特别是悬浮液的细胞培养基制剂，以及使用这些培养基在体外悬浮培养哺乳动物细胞的方法。 培养基包含基础培养基和聚阴离子或聚阴离子化合物，优选多磺化或多硫酸化合物，更优选硫酸葡聚糖。 本发明还提供化学上定义的，无蛋白质的真核细胞培养基，其包含铁螯合物和锌，其能够支持悬浮培养物中的生长（特别是哺乳动物细胞的高密度生长），从而增加表达水平 的培养细胞中的重组蛋白，和/或增加培养细胞中的病毒产生。
-
公开(公告)号：US06578191B1
公开(公告)日：2003-06-10
申请号：US09312888
申请日：1999-05-17
IPC分类号： G06F944
摘要： This invention provides for the implementation of dynamic “event to method” adapter class generation. Event to method adapter classes and objects are automatically and dynamically generated and wired to source and target objects as required using a runtime environments language such as the Beans Markup Language (BML). Adapter classes and objects are automatically and dynamically generated as required while the application program loads and runs. Dynamically generated classes and objects need to exist only at the time that a running application calls for the adapters use, and can be dynamically modified or exchanged in order to optimize the running application or modify application functionality.
摘要翻译： 本发明提供了动态“事件到方法”适配器类生成的实现。 方法适配器类和对象的事件将根据需要使用运行时环境语言（如豆标记语言（BML））自动和动态生成并连接到源对象和目标对象。 适配器类和对象在应用程序加载和运行时根据需要自动和动态生成。 动态生成的类和对象仅在运行的应用程序要求适配器使用时才存在，并且可以动态修改或交换，以便优化正在运行的应用程序或修改应用程序功能。
-
-
-
-
-
-
-
-
-
搜索结果
20 条记录 (耗时 0.027 秒)
