公开(公告)号：US07390461B2

公开(公告)日：2008-06-24

申请号：US10428785

申请日：2003-05-05

申请人： David G. Grier ,  Lewis Gruber

发明人： David G. Grier ,  Lewis Gruber

IPC分类号： G01N21/00 ,  G01N33/545 ,  B32B5/16 ,  A61B17/20 ,  B01J19/08 ,  G02B5/02

CPC分类号： G01N33/54313 ,  G01N21/4788 ,  G01N21/7703 ,  G01N21/7743 ,  G01N33/5436 ,  G03H2001/0044 ,  G03H2001/0077 ,  Y10T428/2991

摘要： The present invention relates to a sensor for detecting chemical, biological, biochemical or other environmental stimuli, and includes a plurality of colloidal particles having chemical receptors bound to each particle's surface, wherein when a target stimulus is directed to the bound chemical receptor, the gelled matrix contracts or expands, which changes the lattice constant, thereby changing the pattern of diffracted light in a measurable manner so that the target stimulus' presence can be detected by a spectrometer. The plurality of colloidal particles are composed of a material having a first dielectric constant, and the gel and surrounding medium have a second dielectric constant. The colloidal particles can be assembled using holographic optical tweezers and polymerized to create an integrated sensor array, which diffracts light in a detectable characteristic manner.

摘要翻译： 本发明涉及一种用于检测化学，生物，生物化学或其他环境刺激的传感器，并且包括具有结合到每个颗粒表面的化学受体的多个胶体颗粒，其中当靶刺激指向结合的化学受体时，胶凝 矩阵收缩或扩展，其改变晶格常数，从而以可测量的方式改变衍射光的图案，使得目标刺激的存在可以由光谱仪检测。 多个胶体颗粒由具有第一介电常数的材料构成，凝胶和周围介质具有第二介电常数。 可以使用全息光学镊子组装胶体颗粒并进行聚合以产生集成的传感器阵列，其以可检测的特征方式衍射光。

公开(公告)号：US07241988B2

公开(公告)日：2007-07-10

申请号：US10630904

申请日：2003-07-31

申请人： Lewis Gruber ,  Kenneth Bradley ,  Ward Lopes ,  Robert W. Lancelot ,  Joseph S. Plewa ,  David G. Grier

发明人： Lewis Gruber ,  Kenneth Bradley ,  Ward Lopes ,  Robert W. Lancelot ,  Joseph S. Plewa ,  David G. Grier

IPC分类号： G01N21/63

CPC分类号： G01N15/1459 ,  B01L3/5027 ,  G01N2015/149 ,  G02B21/32 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2001/085 ,  G03H2225/32 ,  H05H3/04

摘要： The present invention employs a beam steering apparatus to isolate valuable cells from other cells, tissues, and contaminants. In one embodiment, the system balances optical trapping against biasing flow to parallelize cell sorting under the flexible control of computer program-directed traps which differentially manipulate cells based on their composition or labels to direct separation.

摘要翻译： 本发明采用光束转向装置来将有价值的细胞与其它细胞，组织和污染物分离。 在一个实施例中，系统平衡光学捕获与偏置流平衡，以在计算机程序导向的陷阱的灵活控制下平行化细胞分选，其基于它们的组成或标签差异地操纵细胞以引导分离。

公开(公告)号：US09649376B2

公开(公告)日：2017-05-16

申请号：US13876157

申请日：2011-09-27

申请人： Lewis Gruber

发明人： Lewis Gruber

IPC分类号： C07K16/44 ,  A61K39/395 ,  A61K41/00 ,  A61B17/32

CPC分类号： A61K41/0023 ,  A61B17/320068 ,  A61K39/39533 ,  A61K39/3955 ,  C07K16/44

摘要： A method of treating atherosclerosis comprises removing AGE-modified cells from a patient. The AGE-modified cells include erythrocytes, intima cells, endothelial cells, smooth muscle cells, macrophages, and foam cells. A variety of techniques, such as ultrasound and binding with an anti-AGE antibody, may be used to identify and remove the AGE-modified cells.

公开(公告)号：US09161810B2

公开(公告)日：2015-10-20

申请号：US13332976

申请日：2011-12-21

申请人： Lewis Gruber

发明人： Lewis Gruber

IPC分类号： A61K39/395 ,  A61B18/18 ,  A61B18/20 ,  A61B18/00 ,  A61N5/10 ,  A61N7/02

CPC分类号： C07K16/18 ,  A61B18/18 ,  A61B18/1815 ,  A61B18/20 ,  A61B2018/0047 ,  A61K39/395 ,  A61K47/6803 ,  A61K47/6843 ,  A61N5/10 ,  A61N7/00 ,  A61N7/02 ,  C07K2317/21 ,  C07K2317/73 ,  G01N33/5091

摘要： Apparatuses, compositions and methods for removing cells which interfere with regenerative processes. The apparatuses, compositions and methods selectively kill partially functional and/or non-functional cells versus functional cells while protecting functional proliferative cells to the extent that, upon removal of the killed cells by disintegration or scavenging, functional cells replace the partially- or non-functional cells.

摘要翻译： 用于去除干扰再生过程的细胞的装置，组合物和方法。 所述装置，组合物和方法选择性地杀死部分功能性细胞和/或非功能性细胞相对于功能性细胞，同时保护功能性增殖细胞达到通过分解或清除去除所述杀死的细胞的功能性细胞取代部分或非 - 功能细胞。

公开(公告)号：US07699767B2

公开(公告)日：2010-04-20

申请号：US12213109

申请日：2008-06-13

申请人： Daniel Mueth ,  Joseph Plewa ,  Jessica Shireman ,  Amy Anderson ,  Lewis Gruber ,  Neil Harris Rosenbaum

发明人： Daniel Mueth ,  Joseph Plewa ,  Jessica Shireman ,  Amy Anderson ,  Lewis Gruber ,  Neil Harris Rosenbaum

IPC分类号： B04B7/08

CPC分类号： G03H1/0005 ,  A61L2/08 ,  B01D21/0009 ,  B01D21/01 ,  B01D21/262 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0454 ,  B03C1/00 ,  B03C1/002 ,  B03C1/288 ,  B03C5/005 ,  B04B13/00 ,  G01N15/0205 ,  G01N21/6486 ,  G01N27/447 ,  G01N27/44756 ,  G01N33/487 ,  G01N33/5091 ,  G01N2015/0053 ,  G01N2201/061 ,  G01N2458/00 ,  G02B21/32 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2001/085 ,  G03H2225/32 ,  G21K5/08 ,  H05H3/04

摘要： The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

公开(公告)号：US20050122550A1

公开(公告)日：2005-06-09

申请号：US10974976

申请日：2004-10-28

申请人： Joseph Plewa ,  Evan Tanner ,  Dan Mueth ,  Lewis Gruber ,  Kenneth Bradley

发明人： Joseph Plewa ,  Evan Tanner ,  Dan Mueth ,  Lewis Gruber ,  Kenneth Bradley

IPC分类号： G02B5/32 ,  G03H1/08 ,  G03H1/22 ,  G03H1/10

CPC分类号： G03H1/22 ,  G02B5/32 ,  G03H1/0005 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2001/2284

摘要： A method an apparatus for manipulating particles (micro, nano, and pico) having one or more characteristics with an optical trap formed by modulating a laser beam with a Diffractive Optical Element (DOE). At least one characteristic of the material is selected; and a laser beam having a selected wavelength corresponding to the at least one selected characteristic of the material is generated. Values of the DOE are calculated corresponding to the at least one selected characteristic of the material. The beam and the DOE are modulated to produce a holographic optical trap having properties corresponding to the at least one selected characteristic; the trap is focused to a beam focus or selected spot size; and the beam focus is located near a particle location for trapping the particle therein.

公开(公告)号：US20130243785A1

公开(公告)日：2013-09-19

申请号：US13876157

申请日：2011-09-27

申请人： Lewis Gruber

发明人： Lewis Gruber

IPC分类号： A61K39/395 ,  A61B17/32

CPC分类号： A61K41/0023 ,  A61B17/320068 ,  A61K39/39533 ,  A61K39/3955 ,  C07K16/44

摘要： A method of treating atherosclerosis comprises removing AGE-modified cells from a patient. The AGE-modified cells include erythrocytes, intima cells, endothelial cells, smooth muscle cells, macrophages, and foam cells. A variety of techniques, such as ultrasound and binding with an anti-AGE antibody, may be used to identify and remove the AGE-modified cells.

摘要翻译： 治疗动脉粥样硬化的方法包括从患者中除去AGE修饰的细胞。 AGE修饰的细胞包括红细胞，内膜细胞，内皮细胞，平滑肌细胞，巨噬细胞和泡沫细胞。 可以使用多种技术，例如超声和与抗AGE抗体的结合来鉴定和去除AGE修饰的细胞。

公开(公告)号：US20100216208A1

公开(公告)日：2010-08-26

申请号：US12659277

申请日：2010-03-02

申请人： Daniel Mueth ,  Joseph Plewa ,  Jessica Shireman ,  Amy Anderson ,  Lewis Gruber ,  Neil Harris Rosenbaum

发明人： Daniel Mueth ,  Joseph Plewa ,  Jessica Shireman ,  Amy Anderson ,  Lewis Gruber ,  Neil Harris Rosenbaum

IPC分类号： C12N13/00 ,  C12M1/00

CPC分类号： G03H1/0005 ,  A61L2/08 ,  B01D21/0009 ,  B01D21/01 ,  B01D21/262 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0454 ,  B03C1/00 ,  B03C1/002 ,  B03C1/288 ,  B03C5/005 ,  B04B13/00 ,  G01N15/0205 ,  G01N21/6486 ,  G01N27/447 ,  G01N27/44756 ,  G01N33/487 ,  G01N33/5091 ,  G01N2015/0053 ,  G01N2201/061 ,  G01N2458/00 ,  G02B21/32 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2001/085 ,  G03H2225/32 ,  G21K5/08 ,  H05H3/04

摘要： The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

摘要翻译： 本发明提供了一种用于分离血液和其他类型的细胞组分的方法，装置和系统，并且可以与全息光学捕获操作或其他形式的光学镊子组合。 示例性方法之一包括提供具有多个血液成分的第一流; 提供第二流; 使第一流与第二流接触以提供第一分离区; 以及将所述多个血液成分的第一血细胞成分差异沉积到所述第二流中，同时在所述第一流中同时维持所述多个血液成分的第二血细胞成分。 然后，具有第一血细胞成分的第二流与具有第二血细胞成分的第一流有差别地移除。 全息光学捕集器也可以与各种流动结合使用，以将选定的组分从一个流移动到另一个流，作为分离阶段的一部分或除了分离阶段之外。

公开(公告)号：US20090032449A1

公开(公告)日：2009-02-05

申请号：US12213109

申请日：2008-06-13

申请人： Daniel Mueth ,  Joseph Plewa ,  Jessica Shireman ,  Amy Anderson ,  Lewis Gruber ,  Neil Harris Rosenbaum

发明人： Daniel Mueth ,  Joseph Plewa ,  Jessica Shireman ,  Amy Anderson ,  Lewis Gruber ,  Neil Harris Rosenbaum

IPC分类号： B01D35/30

CPC分类号： G03H1/0005 ,  A61L2/08 ,  B01D21/0009 ,  B01D21/01 ,  B01D21/262 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0454 ,  B03C1/00 ,  B03C1/002 ,  B03C1/288 ,  B03C5/005 ,  B04B13/00 ,  G01N15/0205 ,  G01N21/6486 ,  G01N27/447 ,  G01N27/44756 ,  G01N33/487 ,  G01N33/5091 ,  G01N2015/0053 ,  G01N2201/061 ,  G01N2458/00 ,  G02B21/32 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2001/085 ,  G03H2225/32 ,  G21K5/08 ,  H05H3/04

摘要： The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

摘要翻译： 本发明提供了一种用于分离血液和其他类型的细胞组分的方法，装置和系统，并且可以与全息光学捕获操作或其他形式的光学镊子组合。 示例性方法之一包括提供具有多个血液成分的第一流; 提供第二流; 使第一流与第二流接触以提供第一分离区; 以及将所述多个血液成分的第一血细胞成分差异沉积到所述第二流中，同时在所述第一流中同时维持所述多个血液成分的第二血细胞成分。 然后，具有第一血细胞成分的第二流与具有第二血细胞成分的第一流有差别地移除。 全息光学捕集器也可以与各种流动结合使用，以将选定的组分从一个流移动到另一个流，作为分离阶段的一部分或除了分离阶段之外。

公开(公告)号：US07402131B2

公开(公告)日：2008-07-22

申请号：US11543773

申请日：2006-10-06

申请人： Daniel Mueth ,  Joseph Plewa ,  Jessica Shireman ,  Amy Anderson ,  Lewis Gruber ,  Neil Harris Rosenbaum

发明人： Daniel Mueth ,  Joseph Plewa ,  Jessica Shireman ,  Amy Anderson ,  Lewis Gruber ,  Neil Harris Rosenbaum

IPC分类号： B04B7/08

CPC分类号： A61M1/3681 ,  A61M1/36 ,  A61M1/3693 ,  A61M1/38 ,  A61M2206/11 ,  B01L3/502761 ,  B03C1/28 ,  B03C5/005 ,  B03C2201/02 ,  B03C2201/18 ,  G01N30/0005 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2001/085 ,  Y10T436/25375

摘要： The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

摘要翻译： 本发明提供了一种用于分离血液和其他类型的细胞组分的方法，装置和系统，并且可以与全息光学捕获操作或其他形式的光学镊子组合。 示例性方法之一包括提供具有多个血液成分的第一流; 提供第二流; 使第一流与第二流接触以提供第一分离区; 以及将所述多个血液成分的第一血细胞成分差异沉积到所述第二流中，同时在所述第一流中同时维持所述多个血液成分的第二血细胞成分。 然后，具有第一血细胞成分的第二流与具有第二血细胞成分的第一流有差别地移除。 全息光学捕集器也可以与各种流动结合使用，以将选定的组分从一个流移动到另一个流，作为分离阶段的一部分或除了分离阶段之外。