公开(公告)号：US20060029986A1

公开(公告)日：2006-02-09

申请号：US11231256

申请日：2005-09-20

申请人： David Grainger ,  Paul Kemp

发明人： David Grainger ,  Paul Kemp

IPC分类号： G01N33/542 ,  G01N33/537

CPC分类号： A61K9/0024 ,  A61K31/00 ,  A61K31/135 ,  A61K31/138 ,  A61K31/337 ,  A61K31/38 ,  A61K31/40 ,  A61K31/4025 ,  A61K31/4035 ,  A61K31/407 ,  A61K31/715 ,  A61K47/51 ,  A61K47/6803 ,  A61K47/6809 ,  A61K47/6817 ,  A61K47/6831 ,  A61K47/6843 ,  A61K47/6927 ,  A61K47/6937 ,  A61K47/6957 ,  C07K14/71 ,  C07K16/22 ,  C07K2317/73 ,  G01N33/6893 ,  G01N33/74 ,  G01N2333/495 ,  G01N2800/32

摘要： A method for treating or preventing cardiovascular pathologies by administering a compound of the formula (I): wherein Z is C═O or a covalent bond; Y is H or O(C1-C4)alkyl, R1 and R2 are individually (C1-C4)alkyl or together with N are a saturated heterocyclic group, R3 is ethyl or chloroethyl, R4 is H or together with R3 is —CH2—CH2— or —S—, R5 is I, O(C1-C4)alkyl or H and R6 is 1, O(C1-C4)alkyl or H with the proviso that when R4, R5, and R6 are H, R3 is not ethyl; or a pharmaceutically acceptable salt thereof, effective to activate or stimulate production of TGF-beta to treat and/or prevent conditions such as atherosclerosis, thrombosis, myocardial infarction, and stroke is provided. Useful compounds include idoxifene and salts thereof. Further provided is a method for identifying a compound that is a TGF-beta activator or production stimulator is provided. Another embodiment of the invention is an assay or kit to determine TGF-beta in vitro. Also provided is a therapeutic method comprising inhibiting smooth muscle cell proliferation associated with procedural vascular trauma employing the administration of tamoxifen or structural analogs thereof, including compounds of formula (I).

摘要翻译： 一种治疗或预​​防心血管病变的方法，其通过给予式（I）化合物：其中Z为C-O或共价键; Y是H或O（C 1 -C 4）烷基，R 1和R 2各自独立地是（ C 1 -C 4烷基或与N一起为饱和杂环基，R 3是乙基或氯乙基，R 4 H或与R 3一起为-CH 2 - ， - CH 2 - 或-S-，R 5 是（O）（C 1 -C 4 - ）烷基或H，且R 6是1，O（C _{1个C 1 -C 4烷基或H，条件是当R 4，R 5，R 5， 6是H，R 3不是乙基; 提供有效激活或刺激TGF-β的产生以治疗和/或预防诸如动脉粥样硬化，血栓形成，心肌梗死和中风的病症的药学上可接受的盐或其药学上可接受的盐。 有用的化合物包括异昔芬及其盐。 还提供了鉴定作为TGF-β激活剂或生产刺激剂的化合物的方法。 本发明的另一个实施方案是用于在体外测定TGF-β的测定或试剂盒。 还提供了一种治疗方法，其包括使用给予他莫昔芬或其结构类似物（包括式（I）的化合物）来抑制与程序性血管创伤相关的平滑肌细胞增殖。}

公开(公告)号：US20160257930A1

公开(公告)日：2016-09-08

申请号：US15029164

申请日：2014-10-13

申请人： Paul KEMP ,  Nicholas ALLEN

发明人： Paul Kemp ,  Nicholas Allen

IPC分类号： C12N5/0793

CPC分类号： C12N5/0619 ,  C12N2500/14 ,  C12N2500/38 ,  C12N2501/01 ,  C12N2501/13 ,  C12N2501/40 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/845 ,  C12N2501/999 ,  C12N2506/08

摘要： The invention relates to at least one novel neural cell differentiation medium for the production of neural cells from at least one precursor cell capable of lineage specific differentiation; a method for neural cell differentiation using said medium; the use of said medium and said method for lineage specific differentiation of at least one precursor cell towards a neural cell fate; and a kit of parts comprising said at least one said neural cell differentiation medium.

摘要翻译： 本发明涉及至少一种用于从至少一种能够谱系特异性分化的前体细胞产生神经细胞的新型神经细胞分化培养基; 使用所述培养基的神经细胞分化方法; 所述培养基的使用和所述方法用于至少一种前体细胞对神经细胞命运的谱系特异性分化; 以及包含所述至少一种所述神经细胞分化培养基的试剂盒。

公开(公告)号：US20070255254A1

公开(公告)日：2007-11-01

申请号：US11787340

申请日：2007-04-16

申请人： Jane Atkins ,  Michael Leek ,  Paul Kemp ,  Richard Wolowacz ,  Jeffrey Teumer

发明人： Jane Atkins ,  Michael Leek ,  Paul Kemp ,  Richard Wolowacz ,  Jeffrey Teumer

IPC分类号： A61K35/36

CPC分类号： C12N5/0627 ,  A61K35/12 ,  A61K35/36

摘要： Baldness or hair loss has been treated using pharmaceutical drugs and/or hair transplant surgery. The present invention relates to an improved method for treating baldness. In one aspect, there is provided a method for inducing hair follicle formation in a dermal layer lying beneath an outer skin surface, comprising delivering inductive dermal sheath cells and/or inductive dermal papilla cells directly into the dermal layer using a controlled delivery device. Delivery of the inductive cells need not be into pre-existing pores. Use of a controlled delivery device for the treatment of baldness is a further aspect of the invention.

公开(公告)号：US20100203135A1

公开(公告)日：2010-08-12

申请号：US11886113

申请日：2006-03-14

申请人： Paul Kemp ,  David Shering ,  Andrew Shering ,  Penny Johnson ,  Damian Marshall

发明人： Paul Kemp ,  David Shering ,  Andrew Shering ,  Penny Johnson ,  Damian Marshall

IPC分类号： A61K9/14 ,  C07K14/78 ,  A61K38/39 ,  C07D233/74 ,  C07D307/62 ,  C07C53/128 ,  C07K7/64 ,  C07D213/80 ,  C07D281/10 ,  C07C255/43 ,  A61P17/02 ,  C12P21/02

CPC分类号： C12N5/0698 ,  A61L27/20 ,  A61L27/225 ,  A61L27/24 ,  A61L27/3687 ,  A61L27/3691 ,  A61L27/3804 ,  A61L27/3839 ,  A61L27/3886 ,  A61L27/3895 ,  A61L27/54 ,  A61L27/60 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2501/11 ,  C12N2501/15 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2533/56

摘要： Methods of forming soft connective tissue compositions such as skin equivalents, compositions made by the methods and their uses. In particular, a method of forming a connective tissue equivalent, comprising the steps of: (i) incubating collagen-producing cells in or on a support matrix; (ii) inducing and/or enhancing collagen production by the collagen-producing cells to form a collagenous construct and degradation and replacement of the support matrix; (iii) freeze-drying the construct; and (iv) re-populating the freeze-dried construct with collagen-producing cells and/or epithelial cells and/or endothelial cells and/or mesenchymal cells, thereby forming a connective tissue equivalent, wherein: (a) the collagen-producing cells are substantially fibroblasts; for example human neonatal dermal fibroblasts; (b) the support matrix is a provisional support matrix in which the support matrix is a fibrin matrix, for example formed by thrombin-mediated polymerisation of fibrinogen; and (c) as a result of the collagen production by the collagen-producing cells the provisional fibrin support matrix is digested by the cells and is replaced by collagen, thereby essentially replacing the provisional fibrin matrix with a collagen matrix synthesised in situ by the cells.

摘要翻译： 形成软结缔组织组合物如皮肤当量的方法，通过方法制备的组合物及其用途。 特别地，形成结缔组织当量的方法，包括以下步骤：（i）在载体基质中或载体上孵育胶原产生细胞; （ii）诱导和/或增强胶原产生细胞的胶原产生以形成胶原构建物并降解和置换支持基质; （iii）冷冻干燥该构造体; 和（iv）用胶原产生细胞和/或上皮细胞和/或内皮细胞和/或间充质细胞重新填充冷冻干燥的构建体，从而形成结缔组织当量，其中：（a）胶原产生细胞 基本上是成纤维细胞; 例如人类新生儿真皮成纤维细胞; （b）载体基质是临时支持基质，其中载体基质是纤维蛋白基质，例如通过凝血酶介导的纤维蛋白原聚合形成; 和（c）作为胶原蛋白产生细胞产生胶原的结果，临床纤维蛋白载体基质被细胞消化并被胶原替代，从而基本上用由细胞原位合成的胶原基质代替临时纤维蛋白基质 。

公开(公告)号：US4892243A

公开(公告)日：1990-01-09

申请号：US247224

申请日：1988-09-21

申请人： Michael Long ,  Thomas W. Palone ,  Paul Kemp

发明人： Michael Long ,  Thomas W. Palone ,  Paul Kemp

IPC分类号： B65H20/10 ,  B65H23/02 ,  B65H23/032 ,  B65H23/24

CPC分类号： B65H23/02 ,  B65H23/032 ,  B65H23/24 ,  B65H2406/111

摘要： An air-bearing center-guiding apparatus 10 and a method are disclosed for supporting and laterally center-guiding thin flexible webs of paper or plastic under 15 mils in thickness. The apparatus comprises a web support and guide member 22 having an inner surface and an outer web-facing surface. Parallel rows of elongated spaced-apart guide apertures 34 extend longitudinally along edge regions of the web. Each guide aperture 34 is formed from a cylindrical opening in the web support and guide member 22 and extends substantially perpendicular to the web. Within each aperture a flat-sided dowel 60 is mounted to define a flow passage shaped in cross-section like a segment of a circle 62. Each circular-segment guide aperture 34 has a straight side 54 and a curved side 52 for directing a jet of air against the web edge 56. A plurality of the circular-segment guide apertures 34 develop a guiding force for holding lateral movement of the web to plus or minus 0.001 inch (0.0254 mm). A row of web-support apertures 32, separate from the guide apertures 34, is provided between the rows of guide apertures 34 to form an air bearing for supporting the web.

摘要翻译： 公开了一种空气轴承中心引导装置10和方法，用于支撑和横向中心地引导厚度小于15密耳的纸或塑料的薄柔性幅材。 该装置包括具有内表面和面向外网的表面的腹板支撑件和引导构件22。 细长间隔开的引导孔34的平行排沿腹板的边缘区域纵向延伸。 每个引导孔34由幅材支撑件和引导构件22中的圆柱形开口形成并基本上垂直于幅材延伸。 在每个孔内，平面定位销60被安装以限定如同圆形部分62的横截面形状的流动通道。每个圆形段导向孔34具有直的侧面54和用于引导射流的弯曲侧面52 多个圆形段导向孔34形成用于将幅材的横向运动保持在正或负0.001英寸（0.0254mm）的引导力。 与引导孔34分开的一排腹板支撑孔32设置在引导孔34的行之间，以形成用于支撑腹板的空气轴承。

公开(公告)号：US4889269A

公开(公告)日：1989-12-26

申请号：US254341

申请日：1988-09-21

申请人： Michael Long ,  Thomas W. Palone ,  Paul Kemp

发明人： Michael Long ,  Thomas W. Palone ,  Paul Kemp

IPC分类号： B65H20/10 ,  B65H23/02 ,  B65H23/032 ,  B65H23/24

CPC分类号： B65H23/24 ,  B65H23/02 ,  B65H23/032 ,  B65H2406/111

摘要： An air-bearing center-guiding apparatus is disclosed for supporting and laterally center-guiding thin, flexible webs of paper or plastic under 15 mils in thickness. The apparatus comprises a web support and guide member 10 having an inner surface 58 and an outer web-facing surface 60. The web support and guide member 10 comprises a base member 14 for supporting an inner ring 22, an end cap 38, and a center ring 28 interposed therebetween. Parallel, circumferentially extending rows of circumferentially elongated and spaced-apart guide apertures 62 are provided in the web support and guide member 10 along edge regions of the web. The guide apertures 62 are formed between end surfaces 46 of the center ring 28 and interengaging end surfaces of the inner ring 22 and end cap rings 38. Each guide aperture 62 extends substantially perpendicular to the surface of the web and defines in cross-section an opening having a straight side and a curved side for directing jets of air against the web edges for developing a guiding force for holding lateral movement of the web to plus or minus 0.001 inch (0.0254 mm). A row of support apertures 56, separate from the guide apertures 62, is provided in the center ring 28 between the rows of guide apertures 62 to form an air-bearing for the web.

摘要翻译： 公开了一种空气轴承中心引导装置，用于支撑和横向中心地引导厚度小于15密耳的薄而柔软的纸或塑料幅材。 该装置包括腹板支撑件和引导构件10，该构件具有内表面58和外腹板相对表面60.腹板支撑件和导向构件10包括用于支撑内圈22，端盖38和 中间环28插入其间。 平行的沿周向细长且间隔开的导向孔62的周向延伸的排沿腹板的边缘区域设置在腹板支撑件和引导构件10中。 引导孔62形成在中心环28的端面46和内环22和端盖环38的相互接合的端面之间。每个引导孔62基本上垂直于网的表面延伸并且在横截面中限定 具有直边和弯曲侧的开口，用于将空气的喷射引导到幅材边缘上，用于形成用于将幅材的横向移动保持在正或负0.001英寸（0.0254mm）的引导力。 在引导孔62的行之间的中心环28中设置有与引导孔62分离的一排支撑孔56，以形成腹板的空气轴承。

公开(公告)号：US08765169B2

公开(公告)日：2014-07-01

申请号：US10589150

申请日：2005-02-14

申请人： Paul Kemp ,  Györgyi Talas ,  Jennifer Sutherland ,  Margaret Batten ,  Penelope Ann Johnson ,  Andrew Shering ,  Michael McWhan

发明人： Paul Kemp ,  Györgyi Talas ,  Jennifer Sutherland ,  Margaret Batten ,  Penelope Ann Johnson ,  Andrew Shering ,  Michael McWhan

IPC分类号： A61F13/00 ,  A61K9/70 ,  C12N5/071 ,  C12N5/00 ,  C12N5/02

CPC分类号： A61K35/33 ,  A61K35/34 ,  A61K35/36 ,  A61K45/06 ,  A61L27/3804 ,  A61L27/3895 ,  A61L27/60 ,  G01N33/56966 ,  A61K2300/00

摘要： The present invention relates to compositions and methods for tissue regeneration, particularly for treating skin lesions such as wounds. In one aspect, the invention provides wound healing composition characterized by the higher expression levels of phenotypic marker genes such as apolipoprotein D, matrix metalloprotease (2), collagen 3a1 and smooth muscle actin than the housekeeping gene ribosomal protein L32. The compositions and methods of the invention are useful especially for assisting the process of wound healing, particularly chronic open lesions that are slow to heal or resistant to healing.

摘要翻译： 本发明涉及用于组织再生的组合物和方法，特别是用于治疗皮肤损伤如伤口。 一方面，本发明提供伤口愈合组合物，其特征在于表型标记基因如载脂蛋白D，基质金属蛋白酶（2），胶原蛋白3a1和平滑肌肌动蛋白的表达水平高于持家基因核糖体蛋白L32。 本发明的组合物和方法特别适用于辅助伤口愈合过程，特别是慢愈合或抗愈合的慢性开放病变。

公开(公告)号：US08114670B2

公开(公告)日：2012-02-14

申请号：US11886113

申请日：2006-03-14

申请人： Paul Kemp ,  David Shering ,  Andrew Shering, legal representative ,  Penny Johnson ,  Damian Marshall

发明人： Paul Kemp ,  David Shering ,  Penny Johnson ,  Damian Marshall

IPC分类号： C12N5/00 ,  G01N33/53 ,  A01N63/00 ,  A61K9/14

CPC分类号： C12N5/0698 ,  A61L27/20 ,  A61L27/225 ,  A61L27/24 ,  A61L27/3687 ,  A61L27/3691 ,  A61L27/3804 ,  A61L27/3839 ,  A61L27/3886 ,  A61L27/3895 ,  A61L27/54 ,  A61L27/60 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2501/11 ,  C12N2501/15 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2533/56

摘要： Methods of forming soft connective tissue compositions such as skin equivalents, compositions made by the methods and their uses. In particular, a method of forming a connective tissue equivalent, comprising the steps of: (i) incubating collagen-producing cells in or on a support matrix; (ii) inducing and/or enhancing collagen production by the collagen-producing cells to form a collagenous construct and degradation and replacement of the support matrix; (iii) freeze-drying the construct; and (iv) re-populating the freeze-dried construct with collagen-producing cells and/or epithelial cells and/or endothelial cells and/or mesenchymal cells, thereby forming a connective tissue equivalent, wherein: (a) the collagen-producing cells are substantially fibroblasts; for example human neonatal dermal fibroblasts; (b) the support matrix is a provisional support matrix in which the support matrix is a fibrin matrix, for example formed by thrombin-mediated polymerisation of fibrinogen; and (c) as a result of the collagen production by the collagen-producing cells the provisional fibrin support matrix is digested by the cells and is replaced by collagen, thereby essentially replacing the provisional fibrin matrix with a collagen matrix synthesised in situ by the cells.

摘要翻译： 形成软结缔组织组合物如皮肤当量的方法，通过方法制备的组合物及其用途。 特别地，形成结缔组织当量的方法，包括以下步骤：（i）在载体基质中或载体上孵育胶原产生细胞; （ii）诱导和/或增强胶原产生细胞的胶原产生以形成胶原构建物并降解和置换支持基质; （iii）冷冻干燥该构造体; 和（iv）用胶原产生细胞和/或上皮细胞和/或内皮细胞和/或间充质细胞重新填充冷冻干燥的构建体，从而形成结缔组织当量，其中：（a）胶原产生细胞 基本上是成纤维细胞; 例如人类新生儿真皮成纤维细胞; （b）载体基质是临时支持基质，其中载体基质是纤维蛋白基质，例如通过凝血酶介导的纤维蛋白原聚合形成; 和（c）作为胶原蛋白产生细胞产生胶原的结果，临床纤维蛋白载体基质被细胞消化并被胶原替代，从而基本上用由细胞原位合成的胶原基质代替临时纤维蛋白基质 。

公开(公告)号：US20080226720A1

公开(公告)日：2008-09-18

申请号：US10589227

申请日：2005-02-14

申请人： Paul Kemp ,  Gyorgyi Talas ,  Jennifer Sutherland ,  Margaret Batten ,  Penelope Ann Johnson ,  Andrew Shering ,  Michael McWhan

发明人： Paul Kemp ,  Gyorgyi Talas ,  Jennifer Sutherland ,  Margaret Batten ,  Penelope Ann Johnson ,  Andrew Shering ,  Michael McWhan

IPC分类号： A61K9/00 ,  A61K35/12 ,  A61P17/02

CPC分类号： A61K35/33 ,  A61K35/34 ,  A61K35/36 ,  A61K45/06 ,  A61L27/3804 ,  A61L27/3895 ,  A61L27/60 ,  G01N33/56966 ,  A61K2300/00

摘要： The present invention relates to compositions and methods for tissue regeneration, particularly for treating skin lesions such as wounds. In one aspect, the invention provides a wound healing composition comprising living cells such as fibroblasts within a support matrix such as fibrin, in which the cells have a wound healing phenotype, and in which the composition is single layered and has been incubated for up to about 8 days to allow development of the wound healing phenotype. The compositions and methods of the invention are useful especially for assisting the process of wound healing, particularly chronic open lesions that are slow to heal or resistant to healing.

摘要翻译： 本发明涉及用于组织再生的组合物和方法，特别是用于治疗皮肤损伤如伤口。 在一个方面，本发明提供一种伤口愈合组合物，其包含活细胞，例如载体基质如纤维蛋白中的成纤维细胞，其中细胞具有伤口愈合表型，并且其中组合物是单层的并且已经孵育达 约8天允许发展伤口愈合表型。 本发明的组合物和方法特别适用于辅助伤口愈合过程，特别是慢愈合或抗愈合的慢性开放病变。

公开(公告)号：US20050147652A1

公开(公告)日：2005-07-07

申请号：US10504789

申请日：2003-02-10

申请人： Jane Atkins ,  Michael Leek ,  Paul Kemp ,  Richard Wolowacz ,  Jeffrey Teumer

发明人： Jane Atkins ,  Michael Leek ,  Paul Kemp ,  Richard Wolowacz ,  Jeffrey Teumer

IPC分类号： A61K35/12 ,  A61K35/36 ,  A61P17/14 ,  C12N5/071 ,  A61K7/06 ,  A61K9/70

CPC分类号： C12N5/0627 ,  A61K35/12 ,  A61K35/36

摘要： Baldness or hair loss has been treated using pharmaceutical drugs and/or hair transplant surgery. The present invention relates to an improved method for treating baldness. In one aspect, there is provided a method for inducing hair follicle formation in a dermal layer lying beneath an outer skin surface, comprising delivering inductive dermal sheath cells and/or inductive dermal papilla cells directly into the dermal layer using a controlled delivery device. Delivery of the inductive cells need not be into pre-existing pores. Use of a controlled delivery device for the treatment of baldness is a further aspect of the invention.

摘要翻译： 使用药物和/或头发移植手术治疗秃发或脱发。 本发明涉及一种治疗秃发的改进方法。 在一个方面，提供了一种用于在位于外皮表面下面的真皮层中诱导毛囊形成的方法，其包括使用受控输送装置将诱导性皮肤鞘细胞和/或诱导性真皮乳头细胞直接递送到真皮层中。 感应细胞的传送不需要进入预先存在的毛孔。 用于治疗秃发的受控递送装置的使用是本发明的另一方面。