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    ENGINEERED BINDING PROTEINS
    1.
    发明申请
    ENGINEERED BINDING PROTEINS 审中-公开
    工程结合蛋白

    公开(公告)号：US20080076673A1

    公开(公告)日：2008-03-27

    申请号：US11781188

    申请日：2007-07-20

    申请人： David Wilson ,  Steffen Nock

    发明人： David Wilson ,  Steffen Nock

    IPC分类号： C40B30/04 ,  C07K1/00 ,  C07K14/00 ,  C12N15/00 ,  C40B50/06 ,  C40B40/10 ,  C12N15/11 ,  C12N5/06

    CPC分类号： C12N15/1093 ,  B82Y30/00 ,  C07K14/195 ,  C12N9/0095

    摘要： Engineered binding proteins are provided. In some cases, the parent protein corresponding to the engineered protein has a three-layer swiveling β/β/α domain. In other cases, the parent protein corresponding to the engineered protein has a rubredoxin-like fold. At least one portion of the primary sequence of the engineered protein is determined by an engineering scheme. In some case, the engineered protein is characterized by an ability to bind to a compound that the parent protein does not bind. In some cases, the parent protein is derived from a domain of a chaperonin or a rubredoxin. One form of engineering scheme used is a randomization scheme. A method for making libraries of engineered proteins, all based on a single parent protein is provided. Methods to identify proteins that bind to compounds of interest in libraries of engineered libraries are provided. An array of engineered proteins immobilized on a support is provided. Each engineered protein in the array is a chaperonin domain or a rubredoxin that has been subjected to an engineering scheme.

    摘要翻译： 提供工程结合蛋白。 在某些情况下，对应于工程化蛋白质的亲本蛋白质具有三层旋转β/β/α结构域。 在其他情况下，对应于工程改造的蛋白质的亲本蛋白具有类似于rubredoxin的折叠。 工程化蛋白质的初级序列的至少一部分由工程方案确定。 在某些情况下，工程改造的蛋白质的特征在于与母体蛋白不结合的化合物结合的能力。 在一些情况下，母体蛋白质衍生自伴侣蛋白或红蛋白的结构域。 使用的一种工程方案是一种随机化方案。 提供了基于单亲蛋白的制备工程蛋白的文库的方法。 提供了在工程化文库库中鉴定与目的化合物结合的蛋白质的方法。 提供固定在载体上的工程蛋白阵列。 阵列中的每种工程化蛋白质都是经过工程方案的伴侣蛋白结构域或红霉素。

    Super-humanized antibodies against respiratory syncytial virus
    3.
    发明申请
    Super-humanized antibodies against respiratory syncytial virus 审中-公开
    针对呼吸道合胞病毒的超人源化抗体

    公开(公告)号：US20050288491A1

    公开(公告)日：2005-12-29

    申请号：US11061848

    申请日：2005-02-17

    申请人： David Wilson ,  Steffen Nock ,  James Larrick

    发明人： David Wilson ,  Steffen Nock ,  James Larrick

    IPC分类号： A61K39/42 ,  C07K16/10 ,  C07K16/44

    CPC分类号： C07K16/1027 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565

    摘要： Disclosed herein are humanized antibodies that bind to an epitope on the F protein of respiratory syncytial virus. The humanized antibodies were designed by comparing the canonical CDR structure types of the CDRs from a non-human antibody (HNK20) to the canonical CDR structure types found in the human antibody germline sequences as the basis for selecting human variable region frameworks in a method denoted “super-humanization.” Human antibody variable regions having the same or similar canonical CDR structure types as the non-human CDR provided a subset of candidate sequences from which to select the human frameworks. Chimeric variable regions were made comprising the non-human CDRs grafted in corresponding locations into the human frameworks from the candidate human variable regions. Several humanized antibodies that bind the same antigen as HNK20 and that have low immunogenicity were thereby designed, including examples where the framework sequences have less than 65% amino acid identity to the non-human frameworks.

    摘要翻译： 本文公开了与呼吸道合胞病毒的F蛋白上的表位结合的人源化抗体。 通过将来自非人抗体（HNK20）的CDR的规范CDR结构类型与在人抗体种系序列中发现的规范CDR结构类型进行比较来设计人源化抗体，作为在所述方法中选择人可变区框架的基础 “超人性化”。 具有与非人CDR相同或相似的典型CDR结构类型的人抗体可变区提供了选择人骨架的候选序列的子集。 制备嵌合可变区，其包含从候选人可变区移植到人框架中的相应位置的非人CDR。 由此设计了结合与HNK20相同的抗原并且具有低免疫原性的几种人源化抗体，包括其中框架序列与非人框架具有小于65％氨基酸同一性的实例。

    Display of dimeric proteins on phage
    4.
    发明申请
    Display of dimeric proteins on phage 审中-公开
    在噬菌体上显示二聚体蛋白质

    公开(公告)号：US20050147962A1

    公开(公告)日：2005-07-07

    申请号：US10717735

    申请日：2003-11-19

    申请人： Christopher Wagstrom ,  David Wilson ,  Steffen Nock

    发明人： Christopher Wagstrom ,  David Wilson ,  Steffen Nock

    IPC分类号： C07K16/00 ,  C12Q1/70 ,  C07H21/04 ,  C07K16/18 ,  C12N1/21 ,  C12N15/74

    CPC分类号： C07K16/005

    摘要： Expression vectors for expressing multimeric polypeptides that are anchored on surfaces of genetically replicable packages are disclosed. The expression vectors include a vector segment encoding a polypeptide sequence having three polypeptide segments. One of the segments contains a cleavable peptide sequence cleavable by a proteolytic agent, and another segment has an anchoring peptide sequence for anchoring the multimeric polypeptide to the surface of the genetically replicable package. The cleavable peptide sequence is cleaved by the proteolytic agent and the first segment associates with the third segment to form the multimeric polypeptide. Also disclosed are methods, host cells, and kits employing the expression vectors.

    摘要翻译： 公开了用于表达锚定在遗传可复制包装表面上的多聚体多肽的表达载体。 表达载体包括编码具有三个多肽片段的多肽序列的载体片段。 其中一个片段含有可由蛋白水解酶切割的可切割肽序列，另一个片段具有用于将多聚体多肽锚定于基因可复制包装表面的锚定肽序列。 可切割的肽序列被蛋白水解酶切割，第一个片段与第三个片段缔合以形成多聚体多肽。 还公开了使用表达载体的方法，宿主细胞和试剂盒。

    SURGICAL TOOL
    6.
    发明申请
    SURGICAL TOOL 有权
    手术工具

    公开(公告)号：US20170014131A1

    公开(公告)日：2017-01-19

    申请号：US14934878

    申请日：2015-11-06

    申请人： Keith Edward Martin ,  Frank M. Fago ,  David Wilson

    发明人： Keith Edward Martin ,  Frank M. Fago ,  David Wilson

    IPC分类号： A61B17/10

    CPC分类号： A61B17/1285 ,  A61B17/00234 ,  A61B17/10 ,  A61B17/1227 ,  A61B34/71 ,  A61B2017/00243 ,  A61B2017/00323 ,  A61B2017/00584 ,  A61B2017/0069 ,  A61B2017/2902 ,  A61B2017/2927 ,  A61B2017/2937 ,  A61B2017/2939

    摘要： A medical instrument including a first joint including a first member and a second member, the first member configured to be repositionable with respect to the second member in a first degree of freedom, a second joint operatively coupled to the first joint, the second joint including a third member and a fourth member, the third member configured to be repositionable with respect to the fourth member in a second degree of freedom, a pair of repositionable jaws operatively coupled to the first joint and the second joint, an open-ended occlusion clip detachably mounted to the pair of repositionable jaws, and a controller operatively coupled to the first joint, the second joint, and the pair of repositionable jaws, the controller including a control with a line operatively coupled to the first and second jaws in at least a gun tackle pulley configuration.

    摘要翻译： 一种医疗器械，包括包括第一构件和第二构件的第一接头，所述第一构件被构造成在第一自由度中可相对于所述第二构件重新定位，第二接头可操作地联接到所述第一接头，所述第二接头包括 第三构件和第四构件，所述第三构件被构造成能够以第二自由度相对于所述第四构件重新定位，可操作地联接到所述第一接头和所述第二接头的一对可重定位爪，开口闭塞夹 可拆卸地安装到一对可重定位爪上，以及控制器，其可操作地联接到第一接头，第二接头和一对可重定位爪，控制器包括控制器，该控制器具有可操作地连接到第一和第二钳口的至少一个 枪滑轮配置。

    Automated laboratory system
    8.
    发明授权
    Automated laboratory system 有权
    自动化实验室系统

    公开(公告)号：US08865474B2

    公开(公告)日：2014-10-21

    申请号：US11429888

    申请日：2006-05-08

    申请人： Michael Paschetto ,  Peter Massaro ,  Jeff Boot ,  David Wilson

    发明人： Michael Paschetto ,  Peter Massaro ,  Jeff Boot ,  David Wilson

    IPC分类号： G01N33/559 ,  G01N35/00 ,  G01N27/447 ,  B01L3/02 ,  G01N35/04

    CPC分类号： G01N35/0099 ,  B01L3/0244 ,  B01L3/0275 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44747 ,  G01N27/44782 ,  G01N35/04 ,  Y10T436/25375

    摘要： An automated laboratory system and method allow high-throughput and fully automated processing of materials, such as liquids including genetic materials. The invention includes a variety of aspects that may be combined into a single system. For example, processing may be performed by a plurality of robotic-equipped modular stations, where each modular station has its own unique environment in which processes are performed. Transport devices, such as conveyor belts, may move objects between modular stations, saving movement for robots in the modular stations. Gels used for gel electrophoresis may be extruded, thus decreasing the time needed to form such gels. Robotically-operated well forming tools allow wells to be formed in gels in a registered and accurate way.

    摘要翻译： 自动化实验室系统和方法允许对诸如包括遗传物质在内的液体进行高通量和全自动化处理。 本发明包括可以组合成单个系统的各种方面。 例如，处理可以由多个装有机器人的模块化站来执行，其中每个模块站具有其自己的执行过程的独特环境。 诸如传送带之类的运输设备可以在模块化站之间移动物体，从而节省模块化站中机器人的运动。 可以挤出用于凝胶电泳的凝胶，从而减少形成这种凝胶所需的时间。 机器人操作的成型工具允许以注册和准确的方式在凝胶中形成孔。