摘要:
Heterofunctional crosslinking groups are provided having the formula: wherein W is a covalent core component; L1, L2 and L3 are independently linking groups; X is a non-covalent or reversibly covalent protein tag binder; Y is a activatable covalent linking group; and Z is a protected or unprotected covalent crosslinking group.
摘要翻译:提供具有下式的异官能交联基团:其中W是共价核心组分; L 1,L 2和L 3独立地是连接基团; X是非共价或可逆共价蛋白质标签结合物; Y是可活化的共价连接基团; Z是保护或未保护的共价交联基团。
摘要:
Engineered binding proteins are provided. In some cases, the parent protein corresponding to the engineered protein has a three-layer swiveling β/β/α domain. In other cases, the parent protein corresponding to the engineered protein has a rubredoxin-like fold. At least one portion of the primary sequence of the engineered protein is determined by an engineering scheme. In some case, the engineered protein is characterized by an ability to bind to a compound that the parent protein does not bind. In some cases, the parent protein is derived from a domain of a chaperonin or a rubredoxin. One form of engineering scheme used is a randomization scheme. A method for making libraries of engineered proteins, all based on a single parent protein is provided. Methods to identify proteins that bind to compounds of interest in libraries of engineered libraries are provided. An array of engineered proteins immobilized on a support is provided. Each engineered protein in the array is a chaperonin domain or a rubredoxin that has been subjected to an engineering scheme.
摘要:
Disclosed herein are humanized antibodies that bind to an epitope on the F protein of respiratory syncytial virus. The humanized antibodies were designed by comparing the canonical CDR structure types of the CDRs from a non-human antibody (HNK20) to the canonical CDR structure types found in the human antibody germline sequences as the basis for selecting human variable region frameworks in a method denoted “super-humanization.” Human antibody variable regions having the same or similar canonical CDR structure types as the non-human CDR provided a subset of candidate sequences from which to select the human frameworks. Chimeric variable regions were made comprising the non-human CDRs grafted in corresponding locations into the human frameworks from the candidate human variable regions. Several humanized antibodies that bind the same antigen as HNK20 and that have low immunogenicity were thereby designed, including examples where the framework sequences have less than 65% amino acid identity to the non-human frameworks.
摘要:
Expression vectors for expressing multimeric polypeptides that are anchored on surfaces of genetically replicable packages are disclosed. The expression vectors include a vector segment encoding a polypeptide sequence having three polypeptide segments. One of the segments contains a cleavable peptide sequence cleavable by a proteolytic agent, and another segment has an anchoring peptide sequence for anchoring the multimeric polypeptide to the surface of the genetically replicable package. The cleavable peptide sequence is cleaved by the proteolytic agent and the first segment associates with the third segment to form the multimeric polypeptide. Also disclosed are methods, host cells, and kits employing the expression vectors.
摘要:
A medical instrument including a first joint including a first member and a second member, the first member configured to be repositionable with respect to the second member in a first degree of freedom, a second joint operatively coupled to the first joint, the second joint including a third member and a fourth member, the third member configured to be repositionable with respect to the fourth member in a second degree of freedom, a pair of repositionable jaws operatively coupled to the first joint and the second joint, an open-ended occlusion clip detachably mounted to the pair of repositionable jaws, and a controller operatively coupled to the first joint, the second joint, and the pair of repositionable jaws, the controller including a control with a line operatively coupled to the first and second jaws in at least a gun tackle pulley configuration.
摘要:
The invention relates to a method of 13C-MR detection using an imaging medium comprising hyperpolarised 13C-fructose and to an imaging medium containing hyperpolarised 13C-fructose for use in said method.
摘要:
An automated laboratory system and method allow high-throughput and fully automated processing of materials, such as liquids including genetic materials. The invention includes a variety of aspects that may be combined into a single system. For example, processing may be performed by a plurality of robotic-equipped modular stations, where each modular station has its own unique environment in which processes are performed. Transport devices, such as conveyor belts, may move objects between modular stations, saving movement for robots in the modular stations. Gels used for gel electrophoresis may be extruded, thus decreasing the time needed to form such gels. Robotically-operated well forming tools allow wells to be formed in gels in a registered and accurate way.
摘要:
The present invention, in some embodiments, generally relates to methods of determining a treatment protocol for and/or a prognosis of a patient's recovery from a brain injury. In some embodiments, the brain injury results from a hypoxic event. In some embodiments, methods are provided for determining a measure of the concentration of tau protein in a patient sample containing or suspected of containing tau protein.
摘要:
The present invention generally relates, in some embodiments, to methods of determining a patient's prognosis for recurrence of prostate cancer and/or determining a course of treatment for prostate cancer following a radical prostatectomy.