Method and device for sample preparation
    4.
    发明申请
    Method and device for sample preparation 审中-公开
    样品制备方法和装置

    公开(公告)号:US20060198765A1

    公开(公告)日:2006-09-07

    申请号:US11367082

    申请日:2006-03-02

    IPC分类号: B01L3/00

    摘要: The invention provides a sample preparation device for processing a plurality of fluid samples comprising: (a) a plurality of sample processing chambers connected in parallel, each chamber having an internal surface and inlet and outlet ports; (b) media chambers disposed within each sample processing chamber, each media chamber comprising: (i) a bottom frit attached to and extending across the sample processing chamber; and (ii) a top barrier attached to and extending across the sample processing chamber between the bottom frit and the inlet port, wherein the top barrier, bottom frit and internal surface define a media chamber having a first average cross-sectional area; and (c) a bed of separation medium positioned inside the media chamber. In certain embodiments, the top barrier is a frit and/or the separation medium comprises gel resin beads.

    摘要翻译: 本发明提供一种用于处理多个流体样品的样品制备装置,包括:(a)并联连接的多个样品处理室,每个室具有内表面和入口和出口; (b)设置在每个样品处理室内的介质室,每个介质室包括:(i)连接到并延伸穿过样品处理室的底部玻璃料; 和(ii)在底部玻璃料和入口之间附着并延伸穿过样品处理室的顶部屏障,其中顶部屏障,底部玻璃料和内部表面限定具有第一平均横截面积的介质室; 和(c)位于介质室内部的分离介质床。 在某些实施方案中,顶部阻挡层为玻璃料,和/或分离介质包含凝胶树脂珠粒。

    Method and device for sample preparation
    5.
    发明申请
    Method and device for sample preparation 有权
    样品制备方法和装置

    公开(公告)号:US20060124551A1

    公开(公告)日:2006-06-15

    申请号:US11285531

    申请日:2005-11-21

    IPC分类号: B01D15/08

    摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the use of hydrophobic and/or hydrophilic membranes as frits, and/or by the inclusion of a wad of fibrous material to act as a wicking agent.

    摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,本发明提供了以疏水和/或亲水膜为玻璃料,和/或通过包含纤维材料的纤维作为芯吸剂的特征的方法。

    Method and device for sample preparation
    6.
    发明授权
    Method and device for sample preparation 有权
    样品制备方法和装置

    公开(公告)号:US08148168B2

    公开(公告)日:2012-04-03

    申请号:US12837141

    申请日:2010-07-15

    IPC分类号: G01N1/18

    摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.

    摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,萃取柱由具有低孔的玻璃料组成。 在一些实施方案中,提取塔的玻璃料的孔体积小于提取介质床的间隙体积的小于1微升或小于10%。

    Method and Device for Extracting an Analyte
    7.
    发明申请
    Method and Device for Extracting an Analyte 有权
    提取分析物的方法和装置

    公开(公告)号:US20110192797A1

    公开(公告)日:2011-08-11

    申请号:US13091634

    申请日:2011-04-21

    IPC分类号: B01D15/08

    摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the method involves adjusting the head pressure of the column during the process, or otherwise controlling or regulating the head pressure.

    摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施例中,该方法包括在该过程期间调节柱的头部压力,或以其它方式控制或调节头部压力。

    Method and Device for Sample Preparation
    9.
    发明申请
    Method and Device for Sample Preparation 有权
    样品制备方法和装置

    公开(公告)号:US20110038769A1

    公开(公告)日:2011-02-17

    申请号:US12837141

    申请日:2010-07-15

    IPC分类号: B01L3/02

    摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.

    摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,萃取柱由具有低孔的玻璃料组成。 在一些实施方案中,提取塔的玻璃料的孔体积小于提取介质床的间隙体积的小于1微升或小于10%。

    Method and device for sample preparation
    10.
    发明授权
    Method and device for sample preparation 有权
    样品制备方法和装置

    公开(公告)号:US07875462B2

    公开(公告)日:2011-01-25

    申请号:US12730160

    申请日:2010-03-23

    IPC分类号: C07K1/22

    摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.

    摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,萃取柱由具有低孔的玻璃料组成。 在一些实施方案中,提取塔的玻璃料的孔体积小于提取介质床的间隙体积的小于1微升或小于10%。