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公开(公告)号:US20050045543A1
公开(公告)日:2005-03-03
申请号:US10920922
申请日:2004-08-17
申请人: Douglas Gjerde , Ronald Jones , Allen Burge , Christopher Hanna , Leon Yengoyan
发明人: Douglas Gjerde , Ronald Jones , Allen Burge , Christopher Hanna , Leon Yengoyan
CPC分类号: B01J20/285 , B01D15/22 , B01J20/286 , B01J2220/54 , B01J2220/58 , B01J2220/64 , B01L3/0275 , B01L2200/0631 , B82Y30/00 , G01N1/405 , G01N30/02 , G01N30/56 , G01N2035/1053 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods of storing columns in a wet state.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,本发明提供了在湿状态下储存塔的方法。
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公开(公告)号:US20060124551A1
公开(公告)日:2006-06-15
申请号:US11285531
申请日:2005-11-21
申请人: Douglas Gjerde , Allen Burge , Ronald Jones , Mark Abel
发明人: Douglas Gjerde , Allen Burge , Ronald Jones , Mark Abel
IPC分类号: B01D15/08
CPC分类号: G01N1/40 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the use of hydrophobic and/or hydrophilic membranes as frits, and/or by the inclusion of a wad of fibrous material to act as a wicking agent.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,本发明提供了以疏水和/或亲水膜为玻璃料,和/或通过包含纤维材料的纤维作为芯吸剂的特征的方法。
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公开(公告)号:US20060118491A1
公开(公告)日:2006-06-08
申请号:US11292707
申请日:2005-12-01
申请人: Douglas Gjerde , Liem Nguyen , Ronald Jones , Christopher Holman
发明人: Douglas Gjerde , Liem Nguyen , Ronald Jones , Christopher Holman
IPC分类号: B01D15/08
CPC分类号: C07K1/16 , B01J20/286 , B01J20/287 , B01J20/3244 , B01J2220/54 , B01J2220/58 , B01J2220/64 , B82Y30/00 , C12N15/1006 , G01N1/405 , G01N30/02 , G01N30/466 , G01N30/603 , G01N30/6091 , G01N2030/062 , G01N2035/1053 , B01D15/34 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods and devices for desalting and/or buffer exchange of a sample.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,本发明提供了用于样品的脱盐和/或缓冲液交换的方法和装置。
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公开(公告)号:US08148168B2
公开(公告)日:2012-04-03
申请号:US12837141
申请日:2010-07-15
申请人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
发明人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
IPC分类号: G01N1/18
CPC分类号: G01N1/40 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,萃取柱由具有低孔的玻璃料组成。 在一些实施方案中,提取塔的玻璃料的孔体积小于提取介质床的间隙体积的小于1微升或小于10%。
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公开(公告)号:US20110192797A1
公开(公告)日:2011-08-11
申请号:US13091634
申请日:2011-04-21
申请人: Douglas T. Gjerde , Ronald Jones , Allen Burge
发明人: Douglas T. Gjerde , Ronald Jones , Allen Burge
IPC分类号: B01D15/08
CPC分类号: B82Y30/00 , B01D15/325 , B01D15/327 , B01D15/3804 , B01J20/285 , B01J20/291 , B01J2220/64 , B01L3/0275 , B01L2200/0631 , G01N2030/009 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the method involves adjusting the head pressure of the column during the process, or otherwise controlling or regulating the head pressure.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施例中,该方法包括在该过程期间调节柱的头部压力,或以其它方式控制或调节头部压力。
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公开(公告)号:US07722820B2
公开(公告)日:2010-05-25
申请号:US11285531
申请日:2005-11-21
申请人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
发明人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
IPC分类号: B01D15/08
CPC分类号: G01N1/40 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the use of hydrophobic and/or hydrophilic membranes as frits, and/or by the inclusion of a wad of fibrous material to act as a wicking agent.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,本发明提供了以疏水和/或亲水膜为玻璃料,和/或通过包含纤维材料的纤维作为芯吸剂的特征的方法。
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公开(公告)号:US20050019950A1
公开(公告)日:2005-01-27
申请号:US10896382
申请日:2004-07-20
申请人: Douglas Gierde , Ronald Jones , Allen Burge
发明人: Douglas Gierde , Ronald Jones , Allen Burge
CPC分类号: B01L3/0275 , B01D15/325 , B01D15/327 , B01D15/3804 , B01J20/285 , B01J20/291 , B01J2220/64 , B01L2200/0631 , B82Y30/00 , G01N30/6004 , G01N35/10 , G01N2030/009 , G01N2035/103 , G01N2035/1039 , G01N2035/1053 , Y10T436/153333 , Y10T436/25375 , Y10T436/255 , Y10T436/2575 , G01N2030/525
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the method involves adjusting the head pressure of the column during the process, or otherwise controlling or regulating the head pressure.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施例中,该方法包括在该过程期间调节柱的头部压力,或以其它方式控制或调节头部压力。
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公开(公告)号:US09114383B2
公开(公告)日:2015-08-25
申请号:US10920922
申请日:2004-08-17
IPC分类号: G01N30/02 , G01N30/56 , B01J20/285 , B01J20/286 , B01L3/02 , B82Y30/00 , B01D15/22 , G01N1/40 , G01N35/10
CPC分类号: B01J20/285 , B01D15/22 , B01J20/286 , B01J2220/54 , B01J2220/58 , B01J2220/64 , B01L3/0275 , B01L2200/0631 , B82Y30/00 , G01N1/405 , G01N30/02 , G01N30/56 , G01N2035/1053 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods of storing columns in a wet state.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,本发明提供了在湿状态下储存塔的方法。
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公开(公告)号:US20110038769A1
公开(公告)日:2011-02-17
申请号:US12837141
申请日:2010-07-15
申请人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
发明人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
IPC分类号: B01L3/02
CPC分类号: G01N1/40 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,萃取柱由具有低孔的玻璃料组成。 在一些实施方案中,提取塔的玻璃料的孔体积小于提取介质床的间隙体积的小于1微升或小于10%。
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公开(公告)号:US07875462B2
公开(公告)日:2011-01-25
申请号:US12730160
申请日:2010-03-23
申请人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
发明人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
IPC分类号: C07K1/22
CPC分类号: G01N1/40 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,萃取柱由具有低孔的玻璃料组成。 在一些实施方案中,提取塔的玻璃料的孔体积小于提取介质床的间隙体积的小于1微升或小于10%。
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