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公开(公告)号:US20050045543A1
公开(公告)日:2005-03-03
申请号:US10920922
申请日:2004-08-17
申请人: Douglas Gjerde , Ronald Jones , Allen Burge , Christopher Hanna , Leon Yengoyan
发明人: Douglas Gjerde , Ronald Jones , Allen Burge , Christopher Hanna , Leon Yengoyan
CPC分类号: B01J20/285 , B01D15/22 , B01J20/286 , B01J2220/54 , B01J2220/58 , B01J2220/64 , B01L3/0275 , B01L2200/0631 , B82Y30/00 , G01N1/405 , G01N30/02 , G01N30/56 , G01N2035/1053 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods of storing columns in a wet state.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,本发明提供了在湿状态下储存塔的方法。
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公开(公告)号:US20050019951A1
公开(公告)日:2005-01-27
申请号:US10921010
申请日:2004-08-17
申请人: Douglas Gjerde , Allen Burge , Christopher Hanna
发明人: Douglas Gjerde , Allen Burge , Christopher Hanna
IPC分类号: B01D15/32 , B01D15/38 , B01J20/285 , B01J20/291 , B01L3/02 , G01N30/00 , G01N1/10
CPC分类号: B82Y30/00 , B01D15/325 , B01D15/327 , B01D15/3804 , B01J20/285 , B01J20/291 , B01J2220/64 , B01L3/0275 , B01L2200/0631 , G01N2030/009 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the use of verly slow flow rates.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,本发明提供了以使用极慢的流速为特征的方法。
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公开(公告)号:US20050255604A1
公开(公告)日:2005-11-17
申请号:US10920901
申请日:2004-08-17
申请人: Douglas Gjerde , Christopher Hanna , Allen Burge
发明人: Douglas Gjerde , Christopher Hanna , Allen Burge
CPC分类号: B01L3/0275 , B01D15/325 , B01D15/327 , B01D15/3804 , B01J20/285 , B01J20/291 , B01J2220/64 , B01L2200/0631 , B82Y30/00 , G01N30/6004 , G01N35/10 , G01N2030/009 , G01N2035/103 , G01N2035/1039 , G01N2035/1053 , Y10T436/153333 , Y10T436/25375 , Y10T436/255 , Y10T436/2575 , G01N2030/525
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。
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公开(公告)号:US20060198765A1
公开(公告)日:2006-09-07
申请号:US11367082
申请日:2006-03-02
申请人: Douglas Gjerde , Christopher Hanna , Allen Burge
发明人: Douglas Gjerde , Christopher Hanna , Allen Burge
IPC分类号: B01L3/00
CPC分类号: B01J20/26 , B01D15/424 , B01J20/265 , B01J20/28047 , B01J20/291 , B01J20/3242 , B01J2220/54 , B01L3/50255 , B01L2300/0829 , B01L2400/0487 , G01N2030/009
摘要: The invention provides a sample preparation device for processing a plurality of fluid samples comprising: (a) a plurality of sample processing chambers connected in parallel, each chamber having an internal surface and inlet and outlet ports; (b) media chambers disposed within each sample processing chamber, each media chamber comprising: (i) a bottom frit attached to and extending across the sample processing chamber; and (ii) a top barrier attached to and extending across the sample processing chamber between the bottom frit and the inlet port, wherein the top barrier, bottom frit and internal surface define a media chamber having a first average cross-sectional area; and (c) a bed of separation medium positioned inside the media chamber. In certain embodiments, the top barrier is a frit and/or the separation medium comprises gel resin beads.
摘要翻译: 本发明提供一种用于处理多个流体样品的样品制备装置,包括:(a)并联连接的多个样品处理室,每个室具有内表面和入口和出口; (b)设置在每个样品处理室内的介质室,每个介质室包括:(i)连接到并延伸穿过样品处理室的底部玻璃料; 和(ii)在底部玻璃料和入口之间附着并延伸穿过样品处理室的顶部屏障,其中顶部屏障,底部玻璃料和内部表面限定具有第一平均横截面积的介质室; 和(c)位于介质室内部的分离介质床。 在某些实施方案中,顶部阻挡层为玻璃料,和/或分离介质包含凝胶树脂珠粒。
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公开(公告)号:US20060124551A1
公开(公告)日:2006-06-15
申请号:US11285531
申请日:2005-11-21
申请人: Douglas Gjerde , Allen Burge , Ronald Jones , Mark Abel
发明人: Douglas Gjerde , Allen Burge , Ronald Jones , Mark Abel
IPC分类号: B01D15/08
CPC分类号: G01N1/40 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the use of hydrophobic and/or hydrophilic membranes as frits, and/or by the inclusion of a wad of fibrous material to act as a wicking agent.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,本发明提供了以疏水和/或亲水膜为玻璃料,和/或通过包含纤维材料的纤维作为芯吸剂的特征的方法。
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公开(公告)号:US08148168B2
公开(公告)日:2012-04-03
申请号:US12837141
申请日:2010-07-15
申请人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
发明人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
IPC分类号: G01N1/18
CPC分类号: G01N1/40 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,萃取柱由具有低孔的玻璃料组成。 在一些实施方案中,提取塔的玻璃料的孔体积小于提取介质床的间隙体积的小于1微升或小于10%。
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公开(公告)号:US20110192797A1
公开(公告)日:2011-08-11
申请号:US13091634
申请日:2011-04-21
申请人: Douglas T. Gjerde , Ronald Jones , Allen Burge
发明人: Douglas T. Gjerde , Ronald Jones , Allen Burge
IPC分类号: B01D15/08
CPC分类号: B82Y30/00 , B01D15/325 , B01D15/327 , B01D15/3804 , B01J20/285 , B01J20/291 , B01J2220/64 , B01L3/0275 , B01L2200/0631 , G01N2030/009 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the method involves adjusting the head pressure of the column during the process, or otherwise controlling or regulating the head pressure.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施例中,该方法包括在该过程期间调节柱的头部压力,或以其它方式控制或调节头部压力。
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公开(公告)号:US09114383B2
公开(公告)日:2015-08-25
申请号:US10920922
申请日:2004-08-17
IPC分类号: G01N30/02 , G01N30/56 , B01J20/285 , B01J20/286 , B01L3/02 , B82Y30/00 , B01D15/22 , G01N1/40 , G01N35/10
CPC分类号: B01J20/285 , B01D15/22 , B01J20/286 , B01J2220/54 , B01J2220/58 , B01J2220/64 , B01L3/0275 , B01L2200/0631 , B82Y30/00 , G01N1/405 , G01N30/02 , G01N30/56 , G01N2035/1053 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods of storing columns in a wet state.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,本发明提供了在湿状态下储存塔的方法。
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公开(公告)号:US20110038769A1
公开(公告)日:2011-02-17
申请号:US12837141
申请日:2010-07-15
申请人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
发明人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
IPC分类号: B01L3/02
CPC分类号: G01N1/40 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,萃取柱由具有低孔的玻璃料组成。 在一些实施方案中,提取塔的玻璃料的孔体积小于提取介质床的间隙体积的小于1微升或小于10%。
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公开(公告)号:US07875462B2
公开(公告)日:2011-01-25
申请号:US12730160
申请日:2010-03-23
申请人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
发明人: Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
IPC分类号: C07K1/22
CPC分类号: G01N1/40 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , B01D15/3804
摘要: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.
摘要翻译: 本发明提供了用于从样品溶液中纯化分析物(例如生物大分子,如肽,蛋白质或核酸)的提取柱,以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床,通常在两个玻璃料之间。 在一些实施方案中,提取柱使用改进的移液管尖作为柱体。 在一些实施方案中,萃取柱由具有低孔的玻璃料组成。 在一些实施方案中,提取塔的玻璃料的孔体积小于提取介质床的间隙体积的小于1微升或小于10%。
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