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公开(公告)号：US20120238737A1
公开(公告)日：2012-09-20
申请号：US13485592
申请日：2012-05-31
申请人： Douglas J. Dellinger , Agnieszka Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
发明人： Douglas J. Dellinger , Agnieszka Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
CPC分类号： C07H19/073 , C07H13/12 , C07H19/16 , C07H21/02 , Y02P20/55
摘要： Aspects of the invention include 2′ protected nucleoside monomers that are protected at the 2′ site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
摘要翻译： 本发明的方面包括2'受保护的核苷单体，其在2'位置被硫代保护基团保护。 感兴趣的硫代碳保护基包括硫代碳酸酯，硫代碳酸酯，二硫代碳酸酯基，以及硫羰氨基保护基。 本发明的方面还包括包含本发明的保护基团的核酸以及使用本发明的保护基合成核酸的方法。
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2.发明申请公开(公告)号：US20080227964A1
公开(公告)日：2008-09-18
申请号：US11985598
申请日：2007-11-15
IPC分类号： C07H19/00
摘要： Nucleoside monomers, nucleic acids, e.g., oligonucleotides and polynucleotides, methods of making each, methods of deprotecting each, and the like are disclosed herein. Aspects of the invention include 2′ silyl containing thiocarbonate protecting groups. Corresponding compositions and methods are provided.
摘要翻译： 本文公开了核苷单体，核酸，例如寡核苷酸和多核苷酸，制备各种方法，各自的脱保护方法等。 本发明的方面包括2'含甲硅烷基的硫代碳酸酯保护基。 提供了相应的组合物和方法。
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公开(公告)号：US08202983B2
公开(公告)日：2012-06-19
申请号：US12118655
申请日：2008-05-09
申请人： Douglas J. Dellinger , Agnieszka Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
发明人： Douglas J. Dellinger , Agnieszka Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
CPC分类号： C07H19/073 , C07H13/12 , C07H19/16 , C07H21/02 , Y02P20/55
摘要： Aspects of the invention include 2′ protected nucleoside monomers that are protected at the 2′ site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
摘要翻译： 本发明的方面包括2'受保护的核苷单体，其在2'位置被硫代保护基团保护。 感兴趣的硫代碳保护基包括硫代碳酸酯，硫代碳酸酯，二硫代碳酸酯基，以及硫羰氨基保护基。 本发明的方面还包括包含本发明的保护基团的核酸以及使用本发明的保护基合成核酸的方法。
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公开(公告)号：US09067961B2
公开(公告)日：2015-06-30
申请号：US13485592
申请日：2012-05-31
申请人： Douglas J. Dellinger , Agnieszka B. Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
发明人： Douglas J. Dellinger , Agnieszka B. Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
IPC分类号： C07H13/12 , C07H21/02 , C07H19/073 , C07H19/16
CPC分类号： C07H19/073 , C07H13/12 , C07H19/16 , C07H21/02 , Y02P20/55
摘要： Aspects of the invention include 2′ protected nucleoside monomers that are protected at the 2′ site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
摘要翻译： 本发明的方面包括2'受保护的核苷单体，其在2'位置被硫代保护基团保护。 感兴趣的硫代碳保护基包括硫代碳酸酯，硫代碳酸酯，二硫代碳酸酯基，以及硫羰氨基保护基。 本发明的方面还包括包含本发明的保护基团的核酸以及使用本发明的保护基合成核酸的方法。
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公开(公告)号：US20080206850A1
公开(公告)日：2008-08-28
申请号：US11713221
申请日：2007-02-28
摘要： In some embodiments, the present disclosure relates to new phosphoramidite compositions, that have Silyl-containing carbonate or thiocarbonate or ether as 5′-hydroxyl protecting groups useful of the synthesis of DNA, and in particular for the synthesis of long sequences of DNA (e.g., >50 mer). In some embodiments, there are provided methods for simultaneous oxidation of the internucleoside phosphate triester linkages and removal of the 5′-hydroxyl-protecting group, making this process a new 2-step DNA synthesis, that involves the use of peroxyanions in combination with fluoride anions.
摘要翻译： 在一些实施方案中，本公开涉及新的亚磷酰胺组合物，其具有含有甲硅烷基的碳酸酯或硫代碳酸酯或醚作为可用于合成DNA的5'-羟基保护基团，特别是用于合成长序列DNA（例如 ，> 50 mer）。 在一些实施方案中，提供了同时氧化核苷磷酸三酯键并除去5'-羟基保护基团的方法，使该方法成为新的2-步DNA合成，其涉及使用过氧阴离子与氟化物 阴离子
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公开(公告)号：US20080206851A1
公开(公告)日：2008-08-28
申请号：US11713424
申请日：2007-02-28
摘要： In some embodiments, the present disclosure relates to new phosphoramidite compositions, that have Silyl-containing carbonate or thiocarbonate or ether as 5′-hydroxyl protecting groups useful fo the syntheis of RNA, and in particular for the synthesis of long sequences of RNA (e.g., >50 mer). In some embodiments, there are provided methods for simultaneous oxidation of the internucleoside phosphate triester linkages and removal of the 5′-hydroxyl-protecting group, making this process a new 2-step RNA synthesis, that involves the use of peroxyanions in combination with fluoride anions.
摘要翻译： 在一些实施方案中，本公开涉及新的亚磷酰胺组合物，其具有含有甲硅烷基的碳酸酯或硫代碳酸酯或醚作为用于RNA合成的5'-羟基保护基，特别是用于合成长序列的RNA（例如 ，> 50 mer）。 在一些实施方案中，提供了同时氧化核苷磷酸三酯键并除去5'-羟基保护基团的方法，使该方法成为新的2步RNA合成，其涉及使用过氧阴离子与氟化物 阴离子
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7.发明授权Monomer compositions for the synthesis of RNA, methods of synthesis, and methods of deprotection 有权用于合成RNA的单体组合物，合成方法和脱保护方法公开(公告)号：US07759471B2
公开(公告)日：2010-07-20
申请号：US11388112
申请日：2006-03-23
申请人： Douglas J. Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldine Dellinger , Marvin H. Caruthers
发明人： Douglas J. Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldine Dellinger , Marvin H. Caruthers
IPC分类号： C07H19/00 , C07H19/067 , C07H19/167 , C07H21/02
摘要： Nucleotide monomers, polynucleotides, methods of making each, methods of deprotecting each, and the like are disclosed herein.
摘要翻译： 本文公开了核苷酸单体，多核苷酸，制备方法，各自的脱保护方法等。
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公开(公告)号：US08552174B2
公开(公告)日：2013-10-08
申请号：US11387269
申请日：2006-03-23
申请人： Douglas J. Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldine Dellinger , Marvin H. Caruthers , Joel Myerson
发明人： Douglas J. Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldine Dellinger , Marvin H. Caruthers , Joel Myerson
IPC分类号： C07H21/00
摘要： Methods of deprotecting polynucleotides are disclosed. One aspect of the method of deprotecting polynucleotides, among others, includes: providing a polynucleotide, wherein the polynucleotide includes at least one nucleotide monomer that has at least one protecting group selected from the following: a base having a protecting group, a 2′-hydroxyl protecting group, and a combination thereof, and deprotecting at least one of the protecting groups of the polynucleotide by introducing the polynucleotide to a solution including an α-effect nucleophile.
摘要翻译： 公开了脱保护多核苷酸的方法。 多核苷酸脱保护方法的一个方面包括：提供多核苷酸，其中所述多核苷酸包括至少一种具有至少一个选自以下的保护基的核苷酸单体：具有保护基的碱，2'- 羟基保护基团及其组合，并通过将多核苷酸引入到包含α-效应亲核试剂的溶液中来去保护多核苷酸的至少一个保护基团。
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9.发明授权Monomer compositions for the synthesis of polynucleotides, methods of synthesis, and methods of deprotection 有权用于合成多核苷酸的单体组合物，合成方法和脱保护方法公开(公告)号：US08202985B2
公开(公告)日：2012-06-19
申请号：US11387388
申请日：2006-03-23
申请人： Douglas J. Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldino Dellinger , Marvin H. Caruthers
发明人： Douglas J. Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldino Dellinger , Marvin H. Caruthers
IPC分类号： C07H19/067 , C07H19/073 , C07H19/167 , C07H19/173 , C07H21/02 , C07H21/04
摘要： Nucleotide monomers, polynucleotides, methods of making each, and methods of deprotecting each, are disclosed. An embodiment of the nucleotide monomer, among others, includes a nucleotide monomer having a heterobase protecting group selected from structures I through III as described herein. An embodiment of the polynucleotide, among others, includes a plurality of nucleotide moieties having a heterobase protecting group selected from one of structures I through III as described herein.
摘要翻译： 公开了核苷酸单体，多核苷酸，制备方法，以及每种脱保护方法。 核苷酸单体的一个实施方案包括具有选自如本文所述的结构I至III的杂基保护基团的核苷酸单体。 多核苷酸的一个实施方案包括多个具有选自如本文所述的结构I至III之一的异源保护基团的核苷酸部分。
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公开(公告)号：US20090209479A1
公开(公告)日：2009-08-20
申请号：US12118655
申请日：2008-05-09
申请人： Douglas J. Dellinger , Agnieszka Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers
发明人： Douglas J. Dellinger , Agnieszka Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers
CPC分类号： C07H19/073 , C07H13/12 , C07H19/16 , C07H21/02 , Y02P20/55
摘要： Aspects of the invention include 2′ protected nucleoside monomers that are protected at the 2′ site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
摘要翻译： 本发明的方面包括2'受保护的核苷单体，其在2'位置被硫代保护基团保护。 感兴趣的硫代碳保护基包括硫代碳酸酯，硫代碳酸酯，二硫代碳酸酯基，以及硫羰氨基保护基。 本发明的方面还包括包含本发明的保护基团的核酸以及使用本发明的保护基合成核酸的方法。
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