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    Purification of proteins
    1.
    发明授权
    Purification of proteins 有权

    公开(公告)号：US10233211B2

    公开(公告)日：2019-03-19

    申请号：US13955024

    申请日：2013-07-31

    Applicant: EMD Millipore Corporation

    Inventor： Wilson Moya ,  Jad Jaber

    IPC: C07K1/32 ,  C07K1/30

    CPC classification number: C07K1/32 ,  C07K1/30

    Abstract: The present invention relates to a selectively soluble polymer capable of binding to a desired molecules in an unclarified mixture containing various biological materials and the methods of using such a polymer to purify a molecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired molecule (protein, polypeptide, etc) and remains capable of binding to that molecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.

    Removal of microorganisms from fluid samples using nanofiber filtration media
    2.
    发明授权
    Removal of microorganisms from fluid samples using nanofiber filtration media 有权

    公开(公告)号：US10064965B2

    公开(公告)日：2018-09-04

    申请号：US15691286

    申请日：2017-08-30

    Applicant: EMD Millipore Corporation

    Inventor： Mikhail Kozlov ,  Wilson Moya ,  Gabriel Tkacik

    IPC: B01D39/16 ,  A61L2/00 ,  B01D67/00 ,  B01D69/00 ,  C02F1/44

    Abstract: A method for removing microorganisms from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention. Microorganisms such as bacteria, particularly B. Diminuta, are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having a B. Diminuta LRV greater than about 9, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. Another method for removing microorganisms such as bacteria and Mycloplasma, includes passing the liquid through a porous nanofiber containing filtration medium having a microorganism LRV greater than about 8, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. The filtration medium can be in the form of a fibrous electro spun polymeric nanofiber liquid filtration medium mat.

    Purification of Proteins
    4.
    发明申请
    Purification of Proteins 有权
    蛋白质的纯化

    公开(公告)号：US20130317204A1

    公开(公告)日：2013-11-28

    申请号：US13955024

    申请日：2013-07-31

    Applicant: EMD Millipore Corporation

    Inventor： Wilson Moya ,  Jad Jaber

    IPC: C07K1/32

    CPC classification number: C07K1/32 ,  C07K1/30

    Abstract: The present invention relates to a selectively soluble polymer capable of binding to a desired molecules in an unclarified mixture containing various biological materials and the methods of using such a polymer to purify a molecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired molecule (protein, polypeptide, etc) and remains capable of binding to that molecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.

    Abstract translation: 本发明涉及能够在含有各种生物材料的未澄清混合物中结合所需分子的选择性可溶性聚合物以及使用这种聚合物从这种混合物中纯化分子的方法。 聚合物在一定的工艺条件例如pH或温度和/或盐浓度下可溶于混合物中，并且在工艺条件改变时变得不溶解并沉淀出溶液。 聚合物能够结合到所需的分子（蛋白质，多肽等），并且即使在聚合物从溶液中沉淀出来之后仍然能够结合该分子。 然后可以从流的其余部分过滤出沉淀物，并回收所需的生物分子，例如通过洗脱和进一步处理。

    Purification of proteins
    5.
    发明授权
    Purification of proteins 有权

    公开(公告)号：US10793593B2

    公开(公告)日：2020-10-06

    申请号：US13732613

    申请日：2013-01-02

    Applicant: EMD Millipore Corporation

    Inventor： Wilson Moya

    IPC: C07K1/36 ,  C07K1/32 ,  C07K1/30 ,  C07K16/00

    Abstract: The present invention relates to a selectively soluble polymer capable of binding to one or more constituents in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and is rendered insoluble and precipitates out of solution upon a change in the process conditions. While in its solubilized state, the polymer is capable of binding to a selected entity within the stream such as impurities (DNA, RNA, host cell protein, endotoxins, etc.) in a cell broth and remains capable of binding to that entity even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered and further processed.

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